New single crystal hybrid ionic material, ethylenediammonium tetraacetate diperchlorate tetrahydrate (EDTAH₂²⁺.2ClO₄⁻.4H₂O), was synthesized. Its structure was affined by XRD, illustrating P2/n monoclinic system with cell parameters a=11.705(2), b=5.592(11), c=14.250(3)Å, β=101.747(7)° and filling rate Z=2. The 3D-supramolecular network is maintained by weak interactions as confirmed by Hirshfeld surfaces analysis, showing a supramolecular layered-like structure. UV-Vis spectrum reveals that the material is transparent in the visible domain; FT-IR spectroscopy investigates the vibration modes that were correlated to the functional groups of the material. In addition, the hybrid material exhibits significant in vitro antioxidant activity, as demonstrated by the β-carotene bleaching assay, showing effective inhibition of lipid peroxidation compared to BHT. In addition, density functional theory (DFT) calculations provided insight into the electronic structure and showed good agreement with the experimental results, supporting the observed antioxidant behavior.