Objectives: This study aimed to test whether the digital competence of dental hygienists can affect their intraoral scanning potential in terms of scan time and error. Methods: Dental hygienists and dental hygiene students who had never used an intraoral scanner were surveyed to determine their digital competence. Individual data collected using an intraoral scanner was compared with reference data collected using a model scanner to identify scanning errors, and participants’ scanning times were measured. Results: A significant decrease in scanning time was observed as the overall level of digital competence increased. The increase in digital skills and digital knowledge led to a decrease in scanning time by 3.73 and 3.98 minutes, respectively. Conclusions: This study found that digital competence was associated with reduced scan times, but less so with scan errors. This may be due to recent advances in scanning software, and future studies may need to develop a digital competence assessment tool that is more appropriate for the dental field.

Background/Objectives: Forest therapy has demonstrated stress-reducing and immune-enhancing effects, yet its simultaneous impact on cognitive function, stress biomarkers, and oral microbiota in older adults remains unexplored. This study aimed to evaluate the effects of an 8-week community-based village forest experience program on cognitive function, salivary cortisol, and oral pathogenic bacteria in community-dwelling older adults. Methods: A total of 125 older adults (mean age 82.2 ± 5.3 years; 87.2% female) from 17 senior centers participated in a single-arm, pre–post intervention study. Cognitive function was assessed using the Cognitive Impairment Screening Test (CIST), salivary cortisol was measured by ELISA, and seven oral bacterial species were quantified by qPCR. Results: CIST scores improved significantly (p = 0.003, d = 0.27), with the suspected cognitive impairment subgroup showing greater improvement (d = 0.66) and 48.8% transitioning to normal classification. Salivary cortisol decreased significantly (p = 0.002), and total bacterial load, Porphyromonas gingivalis, and Tannerella forsythia were significantly reduced. The 80–84-year age group showed the greatest cognitive gain, whereas participants aged 85 and older showed no significant change. Conclusions: An accessible village forest program may simultaneously benefit cognitive function, stress, and oral health in older adults with early-stage cognitive decline. Controlled studies are needed to confirm causality and elucidate the underlying mechanisms.

Objectives: This pilot trial evaluated the adjunctive effects of combined Lacticaseibacillus rhamnosus DM163 and Lactobacillus plantarum DSR920 following scaling and root planing (SRP) on periodontal parameters, oral microbiota, and safety in patients with mild gingivitis. Methods: Thirty participants with mild gingivitis were enrolled in this single-center, double-blind, randomized pilot trial. After SRP, the participants received either probiotics or a placebo for 12 weeks. Clinical parameters, oral bacteria detected by qPCR, serum inflammatory biomarkers, and safety outcomes were assessed. Results: Twenty-seven participants were included in the analysis (test group n=14, placebo group n=13). No statistically significant between-group or within-group changes were observed in any of the clinical periodontal parameters. Treponema denticola increased in the 6-week mouthwash samples in the placebo group (p=0.005), while Fusobacterium nucleatum increased in the 12-week plaque samples in the test group (p=0.009). Overall, no consistent reduction in major periodontal pathogens was observed. Most serum inflammatory biomarkers remained below the detection limits, and no serious adverse events occurred. Conclusions: In the present post-SRP mild gingivitis setting, the adjunctive use of probiotics was well tolerated but did not demonstrate statistically significant clinical or microbiological effects. Future trials should optimize baseline standardization and statistical power, and incorporate gingival crevicular fluid analysis. Trial registration: KCT0009762.

Background/Objectives: Alzheimer’s disease (AD) exhibits high heritability (60–80%), yet individual-level genetic risk prediction remains challenging. While APOE ε4 is the strongest genetic risk factor, incomplete penetrance complicates risk assessment. Methods: We analyzed seven blood-related members across three generations using the Korean Chip v2.0 genotyping (~1.2 M SNPs) and Illumina EPICv2 DNA methylation profiling. Genetic burden score (GBS) was calculated by summing risk alleles across 320 variants in six AD-associated genes (APOE, PICALM, CLU, CR1, BIN1, and ABCA7). Results: An unexpected pattern was observed in this family: the affected individual (J-003) had the lowest GBS (39 alleles), while individuals with higher genetic burden (51–61 alleles) remained cognitively healthy. J-003 also exhibited lower APOE methylation (β = 0.495) compared to the family mean (β = 0.523). CR1 contributed the most risk alleles across the family, followed by PICALM. Conclusions: This single-case observation cannot establish causality, generalizability, or biological significance. The affected individual’s lower APOE methylation may represent a causal factor, disease consequence, or coincidental variation—scenarios that cannot be distinguished from this dataset. Validation in larger cohorts with multiple affected individuals is required to determine whether integrated multi-omics approaches can inform personalized risk assessment in familial contexts.