Comparative study of phenolic compound composition was performed by HPLC-UV and HPLC-MS techniques using raw materials of Hedysarum alpinum L. plants introduced into the dendrological ecosystem of the Siberian Botanical Garden of Tomsk State University as well as the callus culture of the same species. The callus culture of Hedysarum alpinum was obtained on Murashige and Skoog nutrient medium with the addition of 1.0 mg/L 2,4-D and 0.1 mg/L 6-benzylaminopurine (6-BAP) and was subsequently cultivated on the medium containing α-NAA (2.0 mg/L) and 6-BAP (0.5 mg/L). The culture was characterized by activated phenylpropanoid pathway, which gave rise to the formation of flavonoids, isoflavones, xanthones, and other substances. The total content of flavonoids (calculated on a per quercetin basis) and formononetin in the extracts of callus culture was lower than in the extracts from introduced plants by 35 and 11%, respectively, whereas the content of mangiferin in callus extracts was more than 50% higher than in the extracts of aerial plant parts.

The aim is to evaluate the state of hemostasis and liver system, as well as nitrogen and protein metabolism on the model of direct lung damage in the experiment on 50 mongrel female SD rats weighing 200-250 g. In the experiment, acute respiratory distress syndrome was modeled by a single intratracheal injection of acidine-pepsin with pH-1.2 in the amount of 0.1 ml per animal in the control group (n = 25) and the modeling group (n = 25). Researchers sampled the material after 1, 3, 5, 7, and 14 days (terminal points). Lung damage was assessed by light microscopy on histological preparations. Researchers analyzed serum for biochemical studies, collecting samples from the heart in tubes containing 3.8% sodium citrate at a ratio of 1:9 for coagulation tests in tubes with activator. During the experiment, haemostasis system disturbances accompanied acute respiratory distress syndrome formation, resulting in reduced prothrombin and activated partial thromboplastin time, elevated fibrinogen concentration, and decreased antithrombin III levels. Moreover, it led to the development of multi-organ failure, as evidenced by the recorded deviations of biochemical and coagulation parameters. The combination of multiple pathological processes within a syndrome is the greatest challenge in the treatment process. The development of experimental models to identify the interrelationship of the largest number of significant systems will allow the creation of an optimal model reproducing the entire symptom complex for the development of new protocols to treat acute respiratory distress syndrome.

Проведено сравнительное изучение состава фенольных соединений в растительном сырье копеечника альпийского (Hedysarum alpinum L.), интродуцированного на экосистемной дендрологической территории Сибирского ботанического сада ТГУ, и его каллусной культуры с использованием методов ВЭЖХ-УФ и ВЭЖХ-МС. Установлено, что в полученной на питательной МС-среде с добавлением 1.0 мг/л 2,4-Д и 0.1 мг/л 6-БАП и последующим культивированием на среде с α-НУК (2.0 мг/л) + 6-БАП (0.5 мг/л) каллусной культуре копеечника происходит активация фенилпропаноидного пути с образованием флавоноидов, изофлавонов, ксантонов и др. В результате сравнительного анализа установлено, что в извлечениях каллусной культуры содержание суммы флавоноидов (в пересчете на кверцетин) и формононетина ниже на 35 и 11% соответственно, а содержание мангиферина превышает более чем на 50% в сравнении с извлечением из интродуцированного растения.

Изучена генотоксичность стандартизированного по количеству и соотношению фуро-кумаринов экстракта из клеточной культуры болиголова пятнистого in vivo в метафазных пластинках костного мозга мышей линии СВА и соматических клетках D. melanogaster. Определено, что применение экстракта клеточной культуры болиголова пятнистого не индуцирует генетических повреждений у мышей линии СВА и D. melanogaster, что является одним из объективных критериев безопасности его применения. Полученные результаты определяют возможные перспективы продолжения исследований в плане разработки нового лекарственного препарата растительного...

Xanthotoxin (8-methoxypsoralen) belongs to the group of naturally occurring furanocoumarin (furocoumarin) compounds and is a product of plant secondary metabolism. Analysis of the available literature indicates that xanthotoxin exhibits a broad spectrum of pharmacological activities, including anti-inflammatory, antioxidant, immunomodulatory, and antibacterial effects. Xanthotoxin has been shown to stimulate autophagy via inhibition of the AKT/mTOR pathway, as well as to block cell migration by modulating RIG-1 and NF-κB signaling. Moreover, its effects on JNK/MAPK, PI3K/AKT, Calcium–CaMYK/PYK2, and other signaling cascades have been confirmed. Among its most promising properties is the ability to inhibit ABC transporters, thereby preventing the reduction of chemotherapeutic agent concentrations within tumor cells and enhancing their intracellular accumulation. Thus, the aim of this study was to evaluate xanthotoxin as a potential anticancer agent. The literature review was based on publications indexed in Google Scholar, Scopus, Web of Science, and PubMed and published between 2010 and 2026. Studies describing the biological properties of xanthotoxin, its toxicity, anticancer mechanisms of action, and modulation of ABC transporters were included. This literature review summarizes the pharmacological profile of xanthotoxin, and its biological activities and therapeutic potential, as well as its antitumor effects in various cancer cell lines. The available evidence may provide a foundation for the future development of xanthotoxin as a lead compound for anticancer drug discovery.