Sahariya, a primitive tribal group, residing in Gwalior and Sheopur districts of Madhya Pradesh, India, show high incidence and prevalence of pulmonary tuberculosis (PTB). The study was designed to understand the genetic diversity and phenotype - genotype association of drug resistant M. tuberculosis strains, infecting Sahariya tribe. A total of 103 pulmonary tuberculosis patients from Sahariya tribe were recruited from Gwalior and Sheopur districts. Sputum samples were collected and cultured on LJ slants and drug sensitivity tests were carried out. Genomic DNA was extracted, followed by spoligotyping and genotyping of drug target genes, katG and rpoB, using MAS-PCR, PCR-RFLP and DNA sequencing. Seventeen different spoligotypes were identified, in which, EAI3_IND/ST11 M. tuberculosis strain appeared predominant, followed by CAS1_Delhi/ST26. Results of our phenotypic drug susceptibility test identified high incidence (12.6%) of isoniazid-resistant tuberculosis, while 4.85% isolates were multi drug resistant (MDR). Further genotyping of drug target genes identified 8.7% of isoniazid-R isolates to have a mutation at katG codon 463, while 3.8% isolates showed mutations at two sites, katG codons 315 and 463. In case of MDR-TB isolates, all from CAS lineage, 3.85% had mutations on katG and rpoB genes, at codon 463 and codon 526, respectively, while 0.97% isolates were harbouring mutations at codons 315, 463 and 531. Our findings have revealed that EAI3_IND strain is the predominant strain infecting Sahariya. The incidence of isoniazid-R M. tuberculosis strain infection is high, with an increased propensity to evolve into MDR-TB. Therefore, the TB centres should also consider isoniazid-R status of the isolates along with CBNAAT before deciding the drug regimen for the patients.

Objective: Glenohumeral (GH) osteoarthritis (OA) is the third most common large joint disease, after hip and knee OA. This study aimed to identify risk factors for GH OA. Methods: We used data from the Dallas Shoulder cohort, including individuals aged 40–85. Those with confirmed GH OA based on X-ray were cases, and those without were controls. Univariate, least absolute shrinkage and selection operator and multivariate analyses identified risk factors, including age, body mass index (BMI), sex, work-related shoulder problems, shoulder disability, dislocation, previous trauma, surgery, smoking, hypertension, diabetes, depression, heart disease, OA, night pain and overall sleep quality. Results: A total of 1827 cases and 1556 controls were identified for GH OA. In univariate analysis, significant associations with GH OA were found for increasing age (>40 to ≤50: OR 3.29, 95% CI 2.44 to 4.45; >50 to ≤60: OR 5.90, 95% CI 4.49 to 7.77; >60 to ≤70: OR 12.18, 95% CI 9.22 to 16.08 and >70: OR 16.54, 95% CI 12.47 to 21.94), higher BMI (≤19: OR 1.44, 95% CI 1.01 to 2.04; >25 to ≤30: OR 1.57, 95% CI 1.32 to 1.86; >30 to ≤35: OR 1.85, 95% CI 1.54 to 2.22 and >35: OR 1.77, 95% CI 1.28 to 2.45), prior shoulder injury (OR 1.30; 95% CI 1.12 to 1.50), shoulder surgery history (OR 0.71; 95% CI 0.57 to 0.87), shoulder pain at night (OR 1.35; 95% CI 1.07 to 1.70) and hypertension (OR 0.70; 95% CI 0.60 to 0.81). In multivariate analysis, significant associations remained for age (>40 to ≤50: OR 2.99, 95% CI 2.21 to 4.06; >50 to ≤60: OR 5.48, 95% CI 4.14 to 7.23; >60 to ≤70: OR 11.22, 95% CI 8.44 to 14.88 and >70: OR 16.65, 95% CI 12.45 to 22.17), BMI (≤19: OR 1.49, 95% CI 1.01 to 2.20; >25 to ≤30: OR 1.45, 95% CI 1.20 to 1.77; >30 to ≤35: OR 1.70, 95% CI 1.39 to 2.09 and >35: OR 1.78, 95% CI 1.25 to 2.55) and previous shoulder trauma (OR 0.80; 95% CI 0.68 to 0.94). Conclusion: We identified increasing age and higher BMI as factors associated with GH OA. Due to the large sample size, many risk factors were assessed. Since the shoulder is not a weight-bearing joint, the BMI-GH OA link is likely molecular and systemic, warranting further investigation. Level of evidence: Prognostic level III.

Background: People living with the human immune deficiency virus (PLHIV) are an important group to address HIV prevention. Mostly, 90% of the HIV cases in children are usually through mother-to-child transmission. Dual contraception (barrier condoms i.e., male, and female condoms) are one of the most effective ways to avoid HIV transmission. Thus, the present study was carried out to establish the predictors associated with the use of dual contraceptives in sexually active HIV positive women in Hossana, Southern Ethiopia. Methods: An institution based unmatched case–control study among randomly selected 312 sexually active HIV positive women was conducted from February 2021 to May 2021. The data were collected through structured questionnaire and anti-retroviral treatment (ART) cards considering the case-to-control ratio of 1:3. The information was coded, entered into Epi-Info7.0 and exported to SPSS 20.0 for further analysis. A P-value < 0.25 in bi-variate analysis was further processed for multi-variate analysis and P-value < 0.05 was considered statistically significant. Results: A response rate of 97.2% was recorded. A significant difference was observed towards the use of dual contraceptives in sexually active HIV positive women living in urban vs rural areas (AOR = 0.28; 95% CI = 0.09–0.84), having sexual intercourse with a regular partner (AOR = 3.77; 95% CI = 1.48–9.55) and taking first initiation to use (AOR = 0.05; 95% CI = 0.02–0.11). Conclusion: The determinants associated with lower use of dual contraceptives were residing in rural areas, sexual intercourse with a regular partner and low initiation rate at first time for use of dual contraceptives. Therefore, we strongly recommend that open discussion about sexually transmitted infections like HIV and their prevention, providing adequate facilities in rural areas can help to prevent HIV transmission and reduce the disease burden. The health professionals are encouraged to organize awareness campaigns in rural areas for use of dual contraceptives among PLHIV.

Background Diabetes a chronic disease requires lifelong medical treatments and life-style adjustment. Hence, it requires dedication towards self-care behavior in multiple domains. We aimed to identify determinants of self-care behavior among patients with diabetes through Health Belief Model Perspective. Methods A cross-sectional study was conducted on 276 patients with simple random sampling technique. The level of self-care behavior on diabetic patients was measured using parameters of physical exercise, diet, medication and blood glucose. Bivariate and multivariable logistic regression analyses were conducted. Results Approximately, 119 (43.1%) of them practiced recommended self-care practices. Patients with more information performed 3 times more self-care (OR-3.07;95%CI 0.19–7.9) than less informed patients. Individuals with high income performed two times more self-care than less income (OR-2.42;95%CI 1.04–17.95). High perceived severity was 8 times more likely to performed for self-care than less perceived severity (OR-8.3,95%CI1.19-16.25). Conclusion Status of self-care practices on diabetic patients is lower than studies in other areas. Training should be focused on perceived severity of diabetes and how to overcome perceived barriers for self-care by increasing the frequency and reach out message on diabetes.

The glenohumeral (GH) joint displays diverse glenoid morphology or wear patterns that affect joint alignment, stability, and function. These morphological variations are influenced by sex, ethnicity, and height, potentially limiting the effectiveness of standardized prosthetics. While previous studies have linked glenoid shape to shoulder instability and bone loss, the demographic and anatomic predictors for glenoid morphology or specific glenoid wear patterns remain unclear. This review aims to identify key demographic and anatomical predictors of glenoid morphology to inform surgical planning and improve total shoulder arthroplasty outcomes. We performed a comprehensive literature search of MEDLINE/PubMed, EMBASE, and Web of Science with a search cutoff date of August 2024. We included the studies that addressed demographic and anatomic predictors, such as age, sex, height, hand dominance, laterality, and glenoid morphology. A methodological quality assessment was conducted independently as per standardized set of criteria known as Newcastle–Ottawa Quality Assessment Scale. We retrieved a total of nine studies that reported on demographic and anatomic predictors and glenoid morphology. Seven of these studies were of good quality, while two were of fair quality. Glenoid size was consistently larger in males and positively correlated with age, height, and weight. We also observed that subtype B2 glenoid is linked to increased humeral osteophyte length in primary GH osteoarthritis. Age, male sex, and height are associated with increased glenoid dimensions and version, while subtype B2 glenoid correlates with advanced GH osteoarthritis and bone loss. B2 glenoid present greater surgical challenges requiring individualized approaches. Larger studies are needed to confirm these predictors and explore genetic and molecular links to disease severity.