Zoonotic diseases, which are transmissible between animals and humans, account for approximately 75% of emerging human infectious diseases. Management of these diseases is crucial for reducing risks to human and animal populations. The Tripartite Zoonoses Guide (TZG), developed by the Tripartite organisations—Food and Agriculture Organization of the United Nations (FAO), World Health Organization (WHO), and World Organisation for Animal Health (WOAH)—identified workforce capacity as vital in delivering a One Health approach to zoonotic disease prevention, preparedness, and response. Most workforce development efforts are sector-specific. The Workforce development for effective management of zoonotic diseases: Operational tool of the Tripartite Zoonoses Guide (WFD OT) was developed collaboratively by the Tripartite to strengthen cross-sectoral and multidisciplinary workforce capacity. A landscape analysis was conducted to inform the development of the WFD OT. WHO coordinated a Technical Working Group (TWG) comprising international experts, which convened monthly to guide the development process. The TWG was responsible for designing and developing the core components of the WFD OT, including the workforce functions, occupations, competencies, training programmes, and supporting tools and resources. Following development, the tool was piloted in three countries and officially launched in December 2024. The WFD OT provides a detailed framework for managing zoonotic diseases, including 36 functions across five phases of disease management, 57 occupations, 133 competencies, and 383 training programmes. It also provides a database of 196 tools and resources to address various aspects of workforce development. The tool is designed to be flexible, offering delivery options that include in-person, online, or a combination of both formats. The tool can be used independently or with support from FAO, WHO, and WOAH and is intended to be integrated into existing national and sub-national broader workforce strategies. Zoonotic diseases present complex One Health challenges that require a competent workforce capable of effective multisectoral collaboration. The WFD OT guides countries to create a comprehensive work plan for strengthening multisectoral workforce capacity.

Risk assessment is critical for managing health threats at the human–animal–environment interface, yet sector-specific approaches can result in fragmented actions. To address this gap, the Joint Risk Assessment Operational Tool (JRA OT), an operational tool of the Tripartite Zoonoses Guide, was developed by the Food and Agriculture Organization of the United Nations, the World Health Organization, and the World Organization for Animal Health. The JRA OT provides a structured framework for joint qualitative risk assessments that integrate multisectoral expertise to identify risk pathways, assess likelihood and impact, and develop consensus-based risk management and communication options. Surveillance systems play a critical role in this process by providing the multisectoral data needed to inform risk assessments, while the JRA process helps identify information gaps and guide the strengthening of integrated One Health surveillance. Implemented in at least 52 countries, the JRA OT has informed national mandates in Indonesia, Viet Nam, and Tanzania, regional strategies in West Africa, and adaptations in Canada. Cascade training has been used to build subnational capacity to facilitate roll out. Sustained leadership commitment, multisectoral coordination, routine applications, local adaptation, and capacity building remain essential for global implementation.