Antimicrobial resistance (AMR) is a recurring global problem, which constantly demands new antimicrobial compounds to challenge the resistance. It is well known that essential oils (EOs) have been known for biological activities including antimicrobial properties. In this study, EOs from seven aromatic plants of Asir region of southwestern Saudi Arabia were tested for their antimicrobial efficacy against four drug resistant pathogenic bacterial isolates (Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli,andStreptococcus typhimurium)and one fungal isolate (Candida albicans). Chemical compositions of EOs were determined by gas chromatography-mass spectrometry (GC-MS). The results revealed that EOs fromMentha cervina,Ocimum basilicum,andOriganum vulgareproved most active against all isolates with inhibitory zone range between 17 and 45 mm. The lowest minimum inhibitory concentration (MIC) of 0.025mg/ml was observed forStaph. aureusandStreptococcus pyogeneswith EO ofOriganum vulgare. All the three EOs showed significant anticandida activity. The results related to EOs fromMentha cervina,Ocimum basilicum,andOriganum vulgaredemonstrated significant antimicrobial efficacy against drug resistant microorganisms.