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Many industrialized areas of the world demand for the nutraceuticals due to rapidly growing health risks linked with higher consumption of processed foods. Tateishi Kazu vegetable soup or miracle soup is widely consumed around the world because of its nutraceutical properties. In the current research, the Tateishi Kazu vegetable soup was made from both organic and nonorganic sources, such as carrot, burdock root, shiitake mushroom, daikon radish, and radish leaves. We analyzed colour, antioxidant properties, cell viability, and mineral and free amino acid contents of both soups. The L ∗ a ∗ b and pH values revealed no drastic changes in the colour of the organic soup stored for 96 hours. The essential amino acids were present in higher amounts in an organic soup compared to the nonorganic soup. Similarly, the total mineral contents of the organic soup were higher than the nonorganic soup; however, potassium was the major mineral in both soups. Higher phenolic contents with elevated 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and superoxide dismutase (SOD) activity were noticed in organic soup. Moreover, both soups showed considerable reduction in cell viability of HepG2 cells tested through the MTT assay. From the present study, we concluded that the organic Tateishi Kazu vegetable soup can be of great importance to food industry due to the presence of viable nutrients and pharmacological properties.
Kalsoom Kalsoom; Muhammad Hamayun; Muhammad Aaqil Khan; Yong-Sung Park; Il-Doo Kim; Dong-Hyun Shin; Amjad Iqbal. Physicochemical Properties and Antioxidant Potential of Tateishi Kazu Vegetable Soup. Journal of Food Quality 2021, 2021, 1 -10.
AMA StyleKalsoom Kalsoom, Muhammad Hamayun, Muhammad Aaqil Khan, Yong-Sung Park, Il-Doo Kim, Dong-Hyun Shin, Amjad Iqbal. Physicochemical Properties and Antioxidant Potential of Tateishi Kazu Vegetable Soup. Journal of Food Quality. 2021; 2021 ():1-10.
Chicago/Turabian StyleKalsoom Kalsoom; Muhammad Hamayun; Muhammad Aaqil Khan; Yong-Sung Park; Il-Doo Kim; Dong-Hyun Shin; Amjad Iqbal. 2021. "Physicochemical Properties and Antioxidant Potential of Tateishi Kazu Vegetable Soup." Journal of Food Quality 2021, no. : 1-10.
Plants are susceptible to various environmental constrains, including heat stress due to their sessile nature. Endophytic fungi can be used as a novel technique to protect crop plants against the injurious effects of thermal stress. Endophytic fungi were isolated from Adiantum capillus-veneris L. and tested against heat stress in Glycine max L. and Helianthus annuus L. The results exhibited increased levels of the plant’s chlorophyll, height and biomass in Aspergillus foetidus (AdR-13) inoculated host crop species. Conversely, a significant decrease in lipid peroxidation and reactive oxygen species (ROS) production was noted in A. foetidus-associated host crop species. Likewise, the amounts of ROS-degrading antioxidants (glutathione reductase (GR), peroxidase (POD), ascorbic acid oxidase (AAO), superoxide dismutase (SOD), catalase (CAT)) as well as phenolics were increased, while the amounts of proline and abscisic acid (ABA) were decreased in fungal-associated test crops. Total lipids, proteins and sugars were noted to be high in A. foetidus-associated test crops. From the results, we concluded that A. foetidus have a role in heat stress mitigation that might help to sustain the production of important crops in the future.
Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Khan; Ayaz Ahmad; Sarah Gul; Ho-Youn Kim; In-Jung Lee. Aspergillus foetidus Regulated the Biochemical Characteristics of Soybean and Sunflower under Heat Stress Condition: Role in Sustainability. Sustainability 2021, 13, 7159 .
AMA StyleIsmail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Khan, Ayaz Ahmad, Sarah Gul, Ho-Youn Kim, In-Jung Lee. Aspergillus foetidus Regulated the Biochemical Characteristics of Soybean and Sunflower under Heat Stress Condition: Role in Sustainability. Sustainability. 2021; 13 (13):7159.
Chicago/Turabian StyleIsmail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Khan; Ayaz Ahmad; Sarah Gul; Ho-Youn Kim; In-Jung Lee. 2021. "Aspergillus foetidus Regulated the Biochemical Characteristics of Soybean and Sunflower under Heat Stress Condition: Role in Sustainability." Sustainability 13, no. 13: 7159.
High temperature is one of the compelling ecological stresses faced by the plants owing to anthropogenic activities of mankind. Recently, endophytic fungi are considered to be a novel tool to counteract the consequences of thermal stress in crops. Owing to the problem, we isolated fungal endophytes from Dryopteris blanfordii and their growth favoring potential in rice seedlings. Endophytic fungi have the proficiency to secrete plant growth stimulating secondary metabolites that help the host plants to restore growth under harsh environments. The isolated Penicillium glabrum (DryR- 30) helped the host plant species, i.e. Glycine max L. and Helianthus annuus L. under thermal stress. P. glabrum associated with G. max and H. annuus exposed to 40ºC not only boosted their host growth attributes (total biomass, plant height and chlorophyll contents), but also significantly curtailed the synthesis of reactive oxygen species and lipids peroxidation. Moreover, the abscisic acid (ABA) and proline concentrations were significantly reduced, while the accumulation of ROS-degrading enzymes, such as catalase (CAT), peroxidase (POD), ascorbic acid oxidase (AAO), glutathione reductase (GR) and superoxide dismutase (SOD) were increased in P. glabrum-associated crops. Additionally, the protein, lipid and sugar contents in tested plants were also improved. These stirring findings suggest that P. glabrum can be applied to food crops as a thermal stress alleviation tool.
Ismail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Sarah Gul; Hamayoon Khan; Khushnood Ur Rehman; Hamida Bibi; In-Jung Lee. Penicillium Glabrum Acted as a Heat Stress Relieving Endophyte in Soybean and Sunflower. Polish Journal of Environmental Studies 2021, 30, 3099 -3110.
AMA StyleIsmail Ismail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, Sarah Gul, Hamayoon Khan, Khushnood Ur Rehman, Hamida Bibi, In-Jung Lee. Penicillium Glabrum Acted as a Heat Stress Relieving Endophyte in Soybean and Sunflower. Polish Journal of Environmental Studies. 2021; 30 (4):3099-3110.
Chicago/Turabian StyleIsmail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Sarah Gul; Hamayoon Khan; Khushnood Ur Rehman; Hamida Bibi; In-Jung Lee. 2021. "Penicillium Glabrum Acted as a Heat Stress Relieving Endophyte in Soybean and Sunflower." Polish Journal of Environmental Studies 30, no. 4: 3099-3110.
This study aimed to evaluate the effect of endophytic fungi isolated from Cucurbita roots on growth, physiological, biochemical and molecular attributes of Solanum lycopersicum L. under Cd and Cr stress. The initial screening experiments on Oryza sativa L. seedlings revealed a plant growth promoting endophyte (Cu-17) out of the 17 isolated strains. The strain was identified as Aspergillus niger by amplifying ITS region of 18 S rDNA. A pot experiment was set to evaluate the effect of A. niger on S. lycopersicum exposed to two different concentrations (100 μg/g and 500 μg/g) of Cd and Cr. A. niger alleviated Cd and Cr stress in S. lycopersicum by reducing its translocation to the aerial parts of the host plant. A. niger also enabled the S. lycopersicum to produce significant amounts of IAA, proline, flavonoids, phenols, catalase (CAT) and ascorbic acid oxidase (AAO), in addition to sugar and proteins. Moreover, A. niger facilitated the S. lycopersicum to induce stress responsive genes (SlGSH1 and SlPCS1) in order to develop tolerance against the Cd and Cr stress. Both SlGSH1 and SlPCS1 genes helped the S. lycopersicum to chelate Cd and Cr and thus mitigate their toxicity. The results, therefore, conclude that A. niger might be used as biofertilizer for healthy and safe crop production in Cd and Cr contaminated soils.
Laila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Muhammad Arif; Anwar Husssin; Sumera Afzal Khan. Endophytic Aspergillus niger reprograms the physicochemical traits of tomato under cadmium and chromium stress. Environmental and Experimental Botany 2021, 186, 104456 .
AMA StyleLaila Aziz, Muhammad Hamayun, Mamoona Rauf, Amjad Iqbal, Muhammad Arif, Anwar Husssin, Sumera Afzal Khan. Endophytic Aspergillus niger reprograms the physicochemical traits of tomato under cadmium and chromium stress. Environmental and Experimental Botany. 2021; 186 ():104456.
Chicago/Turabian StyleLaila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Muhammad Arif; Anwar Husssin; Sumera Afzal Khan. 2021. "Endophytic Aspergillus niger reprograms the physicochemical traits of tomato under cadmium and chromium stress." Environmental and Experimental Botany 186, no. : 104456.
Background In the list of abiotic stresses, salt stress is a main growth retarding factor which affects 7% of rain-fed while 30% worldwide irrigated agriculture. However, various strategies are assumed to manage this problem, but the use of endophytes is cheap and eco-friendly. The goal of this study was to evaluate the behavior of endophytic Aspergillus awamori (EWF) in creating salt tolerance in mung bean in terms of its seedling growth, biochemical indices, antioxidant enzymes, endogenous IAA, and ionic status of the plant. Results The results revealed that the 150 mM of NaCl reduced seedling growth (seedlings’ weight and length; leaves number), chlorophyll contents, and IAA. On the other hand, proline, polyphenols, flavonoids, tannin, lipid peroxidation, catalase, and ascorbate peroxidase were increased. Inoculation of EWF had promoted the mung bean growth under all tested conditions. EWF enhanced the biomass and IAA contents of the mung bean plants under salt stress. Moreover, EWF-associated mung bean seedlings exhibited low accumulation of stress markers, and Cl, Na, Na/K, and Ca/K ratio, whereas higher concentrations of Ca, Mg, K, N, and P in mung bean seedlings. Conclusion The results provided a sustainable approach in using endophytic EWF under salt stress, thus concluded that this fungus can be very handy in mung bean as well as other important crop production in saline areas.
Raid Ali; Humaira Gul; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Mohib Shah; Anwar Hussain; Hamida Bibi; In-Jung Lee. Aspergillus awamori ameliorates the physicochemical characteristics and mineral profile of mung bean under salt stress. Chemical and Biological Technologies in Agriculture 2021, 8, 1 -13.
AMA StyleRaid Ali, Humaira Gul, Muhammad Hamayun, Mamoona Rauf, Amjad Iqbal, Mohib Shah, Anwar Hussain, Hamida Bibi, In-Jung Lee. Aspergillus awamori ameliorates the physicochemical characteristics and mineral profile of mung bean under salt stress. Chemical and Biological Technologies in Agriculture. 2021; 8 (1):1-13.
Chicago/Turabian StyleRaid Ali; Humaira Gul; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Mohib Shah; Anwar Hussain; Hamida Bibi; In-Jung Lee. 2021. "Aspergillus awamori ameliorates the physicochemical characteristics and mineral profile of mung bean under salt stress." Chemical and Biological Technologies in Agriculture 8, no. 1: 1-13.
In the present study, we have isolated endophytic fungi Gliocladium cibotii from Verbena officinalis L. and tested against Oryza sativa L. seedlings for growth promotion. The strain was used to help Glycine max L. and Helianthus annuus L. under thermal stress. Association of G. cibotii with G. max and H. annuus enhanced chlorophyll contents, total biomass and plant height. Also, G. cibotii significantly curtailed lipids peroxidation and ROS (reactive oxygen species) in host species exposed to heat stress (40ºC). Furthermore, the concentration of ROS-degrading enzymes, like ascorbic acid oxidase (AAO), catalase (CAT), glutathione reductase (GR), peroxidase (POD) and superoxide dismutase (SOD) were boosted in test crops, while concentration of proline and ABA were inhibited. The phenolics and nutritional value (total lipids, proteins and sugars) of experimental plants were also enhanced. The results conclude that G. cibotii can be used as a heat stress mitigating weapon for food crops in the future.
Ismail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Muhammad Aaqil Khan; In-Jung Lee. An Endophytic Fungus Gliocladium cibotii Regulates Metabolic and Antioxidant System of Glycine max and Helianthus annuus under Heat Stress. Polish Journal of Environmental Studies 2021, 30, 1631 -1640.
AMA StyleIsmail Ismail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, Muhammad Aaqil Khan, In-Jung Lee. An Endophytic Fungus Gliocladium cibotii Regulates Metabolic and Antioxidant System of Glycine max and Helianthus annuus under Heat Stress. Polish Journal of Environmental Studies. 2021; 30 (2):1631-1640.
Chicago/Turabian StyleIsmail Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; Muhammad Aaqil Khan; In-Jung Lee. 2021. "An Endophytic Fungus Gliocladium cibotii Regulates Metabolic and Antioxidant System of Glycine max and Helianthus annuus under Heat Stress." Polish Journal of Environmental Studies 30, no. 2: 1631-1640.
Coconut (Cocos nucifera) is the emblematic palm of tropical coastal areas all around the globe. It provides vital resources to millions of farmers. In an effort to better understand its evolutionary history and to develop genomic tools for its improvement, a sequence draft was recently released. Here, we present a dense linkage map (8402 SNPs) aiming to assemble the large genome of coconut (2.42 Gbp, 2n = 32) into 16 pseudomolecules. As a result, 47% of the sequences (representing 77% of the genes) were assigned to 16 linkage groups and ordered. We observed segregation distortion in chromosome Cn15, which is a signature of strong selection among pollen grains, favouring the maternal allele. Comparing our results with the genome of the oil palmElaeis guineensisallowed us to identify major events in the evolutionary history of palms. We find that coconut underwent a massive transposable element invasion in the last million years, which could be related to the fluctuations of sea level during the glaciations at Pleistocene that would have triggered a population bottleneck. Finally, to better understand the facultative halophyte trait of coconut, we conducted an RNA-seq experiment on leaves to identify key players of signaling pathways involved in salt stress response. Altogether, our findings represent a valuable resource for the coconut breeding community.
Yaodong Yang; Stéphanie Bocs; Haikuo Fan; Alix Armero; Luc Baudouin; Pengwei Xu; Junyang Xu; Dominique This; Chantal Hamelin; Amjad Iqbal; Rashad Qadri; Lixia Zhou; Jing Li; Yi Wu; Zilong Ma; Auguste Emmanuel Issali; Ronan Rivallan; Na Liu; Wei Xia; Ming Peng; Yong Xiao. Coconut genome assembly enables evolutionary analysis of palms and highlights signaling pathways involved in salt tolerance. Communications Biology 2021, 4, 1 -14.
AMA StyleYaodong Yang, Stéphanie Bocs, Haikuo Fan, Alix Armero, Luc Baudouin, Pengwei Xu, Junyang Xu, Dominique This, Chantal Hamelin, Amjad Iqbal, Rashad Qadri, Lixia Zhou, Jing Li, Yi Wu, Zilong Ma, Auguste Emmanuel Issali, Ronan Rivallan, Na Liu, Wei Xia, Ming Peng, Yong Xiao. Coconut genome assembly enables evolutionary analysis of palms and highlights signaling pathways involved in salt tolerance. Communications Biology. 2021; 4 (1):1-14.
Chicago/Turabian StyleYaodong Yang; Stéphanie Bocs; Haikuo Fan; Alix Armero; Luc Baudouin; Pengwei Xu; Junyang Xu; Dominique This; Chantal Hamelin; Amjad Iqbal; Rashad Qadri; Lixia Zhou; Jing Li; Yi Wu; Zilong Ma; Auguste Emmanuel Issali; Ronan Rivallan; Na Liu; Wei Xia; Ming Peng; Yong Xiao. 2021. "Coconut genome assembly enables evolutionary analysis of palms and highlights signaling pathways involved in salt tolerance." Communications Biology 4, no. 1: 1-14.
Cadmium (Cd) and chromium (Cr) in agricultural soils is becoming a serious threat in tomato. The complete removal of these heavy metals (HMs) from contaminated soil is challenging. However, the use of plant endophytes might be beneficial. The pragmatic role of endophytic fungi Aspergillus flavus (Ch-01) in tomato under Cd and Cr stress was, therefore, investigated. A. flavus enhanced growth attributes in tomato seedlings under Cd and Cr stress. Besides, it remediated Cd and Cr toxicity by reducing their translocation from the roots to the upper part of the tomato plants. In fact, A. flavus associated plants developed tolerance against Cd and Cr toxicity via the expression of SlGSH1 and SlPCS1 genes. Both genes helped in metal chelation and mitigation of Cd and Cr toxicity. From the results, it is concluded that A. flavus is a plant growth promoting and HMs alleviating endophyte, hence it might be used as a biofertilizer.
Laila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Anwar Husssin; Sumera Afzal Khan; Muhammad Irshad; In-Jung Lee. Aspergillus Flavus reprogrammed morphological and chemical attributes of Solanum lycopersicum through SlGSH1 and SlPCS1 genes modulation under heavy metal stress. Journal of Plant Interactions 2021, 16, 104 -115.
AMA StyleLaila Aziz, Muhammad Hamayun, Mamoona Rauf, Amjad Iqbal, Anwar Husssin, Sumera Afzal Khan, Muhammad Irshad, In-Jung Lee. Aspergillus Flavus reprogrammed morphological and chemical attributes of Solanum lycopersicum through SlGSH1 and SlPCS1 genes modulation under heavy metal stress. Journal of Plant Interactions. 2021; 16 (1):104-115.
Chicago/Turabian StyleLaila Aziz; Muhammad Hamayun; Mamoona Rauf; Amjad Iqbal; Anwar Husssin; Sumera Afzal Khan; Muhammad Irshad; In-Jung Lee. 2021. "Aspergillus Flavus reprogrammed morphological and chemical attributes of Solanum lycopersicum through SlGSH1 and SlPCS1 genes modulation under heavy metal stress." Journal of Plant Interactions 16, no. 1: 104-115.
Plants are able to produce a number of compounds that can act as antimicrobial agent, signalling agent, phytotoxin, phytoalexin, etc. The exudates from various plant organs may contain low molecular weight compounds (including sugars, inorganic ions, vitamins, nucleotides, amino acids and phenolics), high molecular weight substances (polysaccharides and enzymes and other proteins) and root border cells (RBCs). The potent bioactive compounds that leach from the plant organs, such as seeds, stems, leaves, flowers and roots can affect the environment in a positive or negative way. For example, root exudate was observed to contribute to the complex set of chemical, physical and biological interactions in the rhizosphere, including root–root, root–nematode, and root–microbe interactions. Root–root, root–nematode and root–microbe interactions are categorized as either positive or negative. In fact, when root exudate provides nutrition to the beneficial microorganisms then the interactions are regarded as positive, while root exudate containing nematocides or antimicrobial agents that can kill nematodes or microbes the interactions are said to be negative. In short, plant exudates may consist of potent bioactive molecules that might act as a growth regulator, antimicrobial, nematicidal, and/or natural herbicide. The isolation, identification and purification of such compounds from plant sources will help in the promotion of green yet sustainable agriculture and control of pollution caused by synthetic chemicals.
Amjad Iqbal; Muhammad Hamayun; Farooq Shah; Anwar Hussain. Role of Plant Bioactives in Sustainable Agriculture. Environment, Climate, Plant and Vegetation Growth 2020, 591 -605.
AMA StyleAmjad Iqbal, Muhammad Hamayun, Farooq Shah, Anwar Hussain. Role of Plant Bioactives in Sustainable Agriculture. Environment, Climate, Plant and Vegetation Growth. 2020; ():591-605.
Chicago/Turabian StyleAmjad Iqbal; Muhammad Hamayun; Farooq Shah; Anwar Hussain. 2020. "Role of Plant Bioactives in Sustainable Agriculture." Environment, Climate, Plant and Vegetation Growth , no. : 591-605.
Today's world needs to control the industrial pollution through smarter ways. Presently, we observed the capacity of soil borne fungi to digest Synozol Red HF–6BN and Synozol Black B. Initially, 86 fungal strains were isolated from soil samples randomly collected from industrial sites. Among these, 31 isolates were capable of dye decolorization on solid media, with SN12f and SN13a isolates showed the highest decolorization capacity. The dye decolorization by both strains was higher (80–95%), when incubated for 120 h under optimized conditions of pH, concentration, nutrient source and temperature. The dye (Synozol red HF–6BN and Synozol black B) decolorization by SN12f isolate was maximum (˃90%) at pH7, whereas the SN13a decolorized 90% of Synozol red HF–6BN and 89% of Synozol black B at pH3. The SN13a and SN12f isolates at 40 mg/L showed de-colorization of 94.71%, 81.4% (for Synozol red HF–6BN) and 90.5%, 84.4% (Synozol black B), respectively. Our isolates also mitigated the toxic effect of azo dyes on the growth of phosphate solubilizing soil bacteria. In fact, the untreated effluent showed toxic effects on the growth of beneficial bacterial by developing zone of inhibition (16.5 mm around Aeromonas spp., 14.5 mm around Sallmonella while 14.25 mm around Citrobacter spp). However, the fungal treated dye was unable to develop zone of inhibition. Laccase activity was positive for both of fungal isolates after incubation on Bassnell Hass Medium (0.0733 U/mL for SN12f and 0.0439 U/mL SN13a). Using molecular approaches (ITS region), SN12f was identified as Aspergillus nidulans, while SN13a as Aspergillus fumigatus. The current study showed that local fungal flora can reclaim the contaminated soils and support the agro-friendly micro-flora.
Sumera Afzal Khan; Saadat Mehmood; Nabeela; Amjad Iqbal; Muhammad Hamayun. Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B. Ecotoxicology and Environmental Safety 2020, 206, 111381 .
AMA StyleSumera Afzal Khan, Saadat Mehmood, Nabeela, Amjad Iqbal, Muhammad Hamayun. Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B. Ecotoxicology and Environmental Safety. 2020; 206 ():111381.
Chicago/Turabian StyleSumera Afzal Khan; Saadat Mehmood; Nabeela; Amjad Iqbal; Muhammad Hamayun. 2020. "Industrial polluted soil borne fungi decolorize the recalcitrant azo dyes Synozol red HF–6BN and Synozol black B." Ecotoxicology and Environmental Safety 206, no. : 111381.
Coconut tissues consist of a complex network of polysaccharides, proteins, polyphenols, lipids that can bind to the nucleic acids and impose difficulty in isolation. Certainly, a vigorous method is required to isolate high quality and quantity of RNA from such tissues for the purpose of downstream experiments. In this paper, we discuss a newly developed method for the Isolation of RNA from Complex Matrices (IRCM) method from coconut tissues. The method is robust, cheap and efficient to extract quality RNA in high quantities from the solid endosperm of stored and fresh coconut (150 μg/g FW with A260/280 = 1.89 and 247.5 μg/g FW with A260/280 = 1.91), coconut apple (263.8 μg/g FW with A260/280 = 1.97) and coconut bud (1052.5 μg/g FW with A260/280 = 2.00), respectively. Whereas the other well established methods, such as Method of RNA Isolation from Palm (MRIP), Cetyl Trimethyl Ammonium Bromide (CTAB), TRIZOL and RNA plant kit were failed to isolate quality RNA in appreciable quantities from the coconut tissues. Furthermore, the resulted RNA performed well in the downstream experiment, i.e. RT-PCR for the production and amplification of cDNA. From the study, we concluded that the present method will play a vital role in the extraction of high quality RNA from complex matrices in short time.
Amjad Iqbal; Yaodong Yang; Yi Wu; Jing Li; Muhammad Hamayun; Anwar Hussain; Farooq Shah. An easy and robust method for the isolation of high quality RNA from coconut tissues. Electronic Journal of Biotechnology 2020, 48, 78 -85.
AMA StyleAmjad Iqbal, Yaodong Yang, Yi Wu, Jing Li, Muhammad Hamayun, Anwar Hussain, Farooq Shah. An easy and robust method for the isolation of high quality RNA from coconut tissues. Electronic Journal of Biotechnology. 2020; 48 ():78-85.
Chicago/Turabian StyleAmjad Iqbal; Yaodong Yang; Yi Wu; Jing Li; Muhammad Hamayun; Anwar Hussain; Farooq Shah. 2020. "An easy and robust method for the isolation of high quality RNA from coconut tissues." Electronic Journal of Biotechnology 48, no. : 78-85.
Drought is a severe environmental constraint, which significantly affects plant growth, productivity, and quality. Plants have developed specific mechanisms that perceive the stress signals and respond to external environmental changes via different mitigation strategies. Abscisic acid (ABA), being one of the phytohormones, serves as an important signaling mediator for plants’ adaptive response to a variety of environmental stresses. ABA triggers many physiological processes, including bud dormancy, seed germination, stomatal closure, and transcriptional and post-transcriptional regulation of stress-responsive gene expression. The site of its biosynthesis and action must be clarified to understand the signaling network of ABA. Various studies have documented multiple sites for ABA biosynthesis, their transporter proteins in the plasma membrane, and several components of ABA-dependent signaling pathways, suggesting that the ABA response to external stresses is a complex networking mechanism. Knowing about stress signals and responses will increase our ability to enhance crop stress tolerance through the use of various advanced techniques. This review will elaborate on the ABA biosynthesis, transportation, and signaling pathways at the molecular level in response to drought stress, which will add a new insight for future studies.
Shahid Ali; Kashif Hayat; Amjad Iqbal; Linan Xie. Implications of Abscisic Acid in the Drought Stress Tolerance of Plants. Agronomy 2020, 10, 1323 .
AMA StyleShahid Ali, Kashif Hayat, Amjad Iqbal, Linan Xie. Implications of Abscisic Acid in the Drought Stress Tolerance of Plants. Agronomy. 2020; 10 (9):1323.
Chicago/Turabian StyleShahid Ali; Kashif Hayat; Amjad Iqbal; Linan Xie. 2020. "Implications of Abscisic Acid in the Drought Stress Tolerance of Plants." Agronomy 10, no. 9: 1323.
In this study, the effect of enzymatic cross-linking of shrimp tropomyosin (TM) with tyrosinase and caffeic acid (TM-Tyr/CA) on the allergic response were assessed using in vitro and in vivo models. The RBL-2H3 and KU812 cell lines were employed to evaluate the changes in the stimulation abilities of TM-Tyr/CA that showed significant inhibition of mediators and cytokines. The digestibility of cross-linked TM was improved and the recognitions of IgG/IgE were markedly reduced, as revealed by western blotting. TM-Tyr/CA decreased anaphylactic symptoms, and hindered the levels of IgG1, IgE, histamine, tryptase and mouse mast-cell protease-1 (mMCP-1) in mice sera. Cross-linked TM downregulated the production of interleukin (IL)-4, IL-5, and IL-13 by 51.36, 12.24 and 20.55%, respectively, whereas, IL-10 and IFN-γ were upregulated by 20.71 and 19.0%. TM-Tyr/CA showed reduced allergenicity and may have preventive effect in relieving TM induced allergic response via immunosuppression and positive modulation of T-helper (Th)1/Th2 immunobalance.
Ishfaq Ahmed; Hong Lin; Zhenxing Li; Lili Xu; Ihsan Mabood Qazi; Chen Luo; Xiang Gao; Mati Ullah Khan; Amjad Iqbal; Yuman Guo; Tushar Ramesh Pavase; Lirui Sun. Tyrosinase/caffeic acid cross-linking alleviated shrimp (Metapenaeus ensis) tropomyosin-induced allergic responses by modulating the Th1/Th2 immunobalance. Food Chemistry 2020, 340, 127948 .
AMA StyleIshfaq Ahmed, Hong Lin, Zhenxing Li, Lili Xu, Ihsan Mabood Qazi, Chen Luo, Xiang Gao, Mati Ullah Khan, Amjad Iqbal, Yuman Guo, Tushar Ramesh Pavase, Lirui Sun. Tyrosinase/caffeic acid cross-linking alleviated shrimp (Metapenaeus ensis) tropomyosin-induced allergic responses by modulating the Th1/Th2 immunobalance. Food Chemistry. 2020; 340 ():127948.
Chicago/Turabian StyleIshfaq Ahmed; Hong Lin; Zhenxing Li; Lili Xu; Ihsan Mabood Qazi; Chen Luo; Xiang Gao; Mati Ullah Khan; Amjad Iqbal; Yuman Guo; Tushar Ramesh Pavase; Lirui Sun. 2020. "Tyrosinase/caffeic acid cross-linking alleviated shrimp (Metapenaeus ensis) tropomyosin-induced allergic responses by modulating the Th1/Th2 immunobalance." Food Chemistry 340, no. : 127948.
Adil Hussain; Amjad Iqbal; Zafar Hayat Khan; Farooq Shah. Introductory Chapter: Recent Advances in Grain Crops Research. Recent Advances in Grain Crops Research 2020, 1 .
AMA StyleAdil Hussain, Amjad Iqbal, Zafar Hayat Khan, Farooq Shah. Introductory Chapter: Recent Advances in Grain Crops Research. Recent Advances in Grain Crops Research. 2020; ():1.
Chicago/Turabian StyleAdil Hussain; Amjad Iqbal; Zafar Hayat Khan; Farooq Shah. 2020. "Introductory Chapter: Recent Advances in Grain Crops Research." Recent Advances in Grain Crops Research , no. : 1.
Plants can be severely affected by heat stress due to rapid environmental changes. Use of endophytic fungi is a new tool to protect crops from the environmental stresses. Here we claim a potent endophyte isolated from Sonchus asper L. The aim was to explore the stress adaptive mechanism of sunflower and soybean mediated by Aspergillus niger (SonchL-7) under high temperature. Inoculation with A. niger boosted plant height, biomass and chlorophyl contents, while significantly curtailed the concentration of lipid peroxidation and reactive oxygen species (ROS) under thermal stress at 40°C. Moreover, the ROS-scavenging activities, like ascorbic acid oxidase (AAO), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD) and peroxidase (POD) were augmented. Also, proline and phenolics were enhanced in the tested crops, while ABA concentration was significantly reduced. These positive results suggested that A. niger can be used as a heat-stress ameliorative tool for crops in the future.
Ismail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; In-Jung Lee. Aspergillus niger boosted heat stress tolerance in sunflower and soybean via regulating their metabolic and antioxidant system. Journal of Plant Interactions 2020, 15, 223 -232.
AMA StyleIsmail, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Sumera Afzal Khan, In-Jung Lee. Aspergillus niger boosted heat stress tolerance in sunflower and soybean via regulating their metabolic and antioxidant system. Journal of Plant Interactions. 2020; 15 (1):223-232.
Chicago/Turabian StyleIsmail; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Sumera Afzal Khan; In-Jung Lee. 2020. "Aspergillus niger boosted heat stress tolerance in sunflower and soybean via regulating their metabolic and antioxidant system." Journal of Plant Interactions 15, no. 1: 223-232.
Oil palm (Elaeis guineensis Jacq.) is a representative tropical oil crop that is sensitive to low temperature. Oil palm can experience cold damage when exposed to low temperatures for a long period. During these unfavorable conditions, a series of gene induction/repression and physico-chemical changes occur in oil palm. To better understand the link between these events, we investigated the expression levels of various genes (including COR410, COR413, CBF1, CBF2, CBF3, ICE1-1, ICE1-2, ICE1-4, SIZ1-1, SIZ1-2, ZAT10, ZAT12) and the accumulation of osmolytes (proline, malondialdehyde and sucrose). Likewise, the activity of superoxide dismutase (SOD) in oil palm under cold stress (4°C, 8°C and 12°C) was examined. The results showed a clear link among the expression of CBFs (especially CBF1 and CBF3) and the all genes examined under cold stress (12°C). The expression of CBF1 and CBF2 also exhibited a positive link with the accumulation of sucrose and proline under cold stress in oil palm. At 4°C, the proline content exhibited a very significant correlation with electrolyte leakage in oil palm. The results of this study provide necessary information regarding the mechanism of the response and adaption of oil palm to cold stress. Additionally, they offer clues for the selection or development of cold-tolerant cultivars from the available germplasms of oil palm.
Jing Li; Yaodong Yang; Amjad Iqbal; Rashad Qadri; Peng Shi; Yong Wang; Yi Wu; Haikuo Fan; Guojiang Wu. Correlation analysis of cold-related gene expression with physiological and biochemical indicators under cold stress in oil palm. PLOS ONE 2019, 14, e0225768 .
AMA StyleJing Li, Yaodong Yang, Amjad Iqbal, Rashad Qadri, Peng Shi, Yong Wang, Yi Wu, Haikuo Fan, Guojiang Wu. Correlation analysis of cold-related gene expression with physiological and biochemical indicators under cold stress in oil palm. PLOS ONE. 2019; 14 (11):e0225768.
Chicago/Turabian StyleJing Li; Yaodong Yang; Amjad Iqbal; Rashad Qadri; Peng Shi; Yong Wang; Yi Wu; Haikuo Fan; Guojiang Wu. 2019. "Correlation analysis of cold-related gene expression with physiological and biochemical indicators under cold stress in oil palm." PLOS ONE 14, no. 11: e0225768.
Besides acting as growth inducing molecule, Gibberellin (GA3) also confers the compatibility of microbial interactions with host. We inoculated 11 days oldZ. maysseedlings grown under hydroponic conditions and high GA3levels withBipolaris sorokiniana(BIPOL) at the spore density (SD) of OD0.6. The high level of GA3negatively affected the growth of the seedlings, accompanied by the high level of stress deducing secondary metabolites (proline, total flavanoids, phenylpropanoids, and glucosinolides). Moreover, high level of GA3produced a hypersensitive response (HR) in the seedlings. The HR developed cross talks with IAA and trans-zeatins and triggered higher production of hypersensitive inducing biomolecules. The other HR co-related biological processes were demonstrated by high phytoalexins level and high protease activities. Such activities ultimately inhibited the colonization of BIPOL on the roots of maize seedlings. The products of the genes expressed at high GA3also conferred the deterrence of BIPOL colonization at SD = OD0.6. Intriguingly, when we inhibited GA3biosynthesis in the seedlings with aerially sprayed uniconizole, prior to BIPOL treatment, the BIPOL colonized and subsequently promoted the seedling growth. This low level of GA3after BIPOL treatment checked the high level of secondary metabolites and hypersensitivity inducing molecules. The results, thus suggested that the aforementioned processes only happened in the BIPOL at SD (OD0.6), whereas the SD at lower levels (OD0.2or OD0.4) neither promoted the growth of uniconizole pre-treated seedlings nor produced HR in control seedlings of maize plant.
Muhammad Junaid Yousaf; Anwar Hussain; Muhammad Hamayun; Amjad Iqbal; Muhammad Irshad; Ayaz Ahmad; In-Jung Lee. Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays. 2019, 847061 .
AMA StyleMuhammad Junaid Yousaf, Anwar Hussain, Muhammad Hamayun, Amjad Iqbal, Muhammad Irshad, Ayaz Ahmad, In-Jung Lee. Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays. . 2019; ():847061.
Chicago/Turabian StyleMuhammad Junaid Yousaf; Anwar Hussain; Muhammad Hamayun; Amjad Iqbal; Muhammad Irshad; Ayaz Ahmad; In-Jung Lee. 2019. "Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays." , no. : 847061.
Fungal endophytes are known to secrete a large array of secondary metabolites (phenols, flavonoids, indole acetic acid (IAA) etc.) that facilitate crops under stress conditions. Considering this, a potent plant growth promoting endophyte (SXSp1) from the spines and leaves of Solanum xanthocarpum L. has been isolated. The isolated strain ably secreted high quantities of indole-3-acetic acid, phenols and flavonoids. Also, it exhibited phosphate solubilization, siderophore and had 2,2 diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The SXSp1 also resisted the salinity stress up to 150 mM. LC/MS analysis of SXSp1 culture filtrate (CF) revealed the presence of p-hydroxyl benzoic acid, diadzein, genistien, myricetin and caffeoyl-d-glucose. Moreover, the inoculation of maize plants with SXSp1 significantly (P=0.05) promoted the chlorophyll and carotenoid contents, root and shoot lengths, plant fresh and dry weights, catalase and peroxidase activities, proline, phenolics, flavonoids and relative water contents (RWCs) under salinity. More interestingly, SXSp1-associated plants showed lower endogenous abscisic acid (ABA) and higher endogenous IAA contents that helped the plants to resist salinity stress up to 100 mM. After sequencing, internal transcribed spacer (ITS) regions (ITS1 and ITS4) and phylogenetic analysis, the SXSp1 was identified as Meyerozyma caribbica.
Farzana Gul Jan; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Gul Jan; Sumera Afzal Khan; Hamayoon Khan; In-Jung Lee. A promising growth promoting Meyerozyma caribbica from Solanum xanthocarpum alleviated stress in maize plants. Bioscience Reports 2019, 39, 1 .
AMA StyleFarzana Gul Jan, Muhammad Hamayun, Anwar Hussain, Amjad Iqbal, Gul Jan, Sumera Afzal Khan, Hamayoon Khan, In-Jung Lee. A promising growth promoting Meyerozyma caribbica from Solanum xanthocarpum alleviated stress in maize plants. Bioscience Reports. 2019; 39 (10):1.
Chicago/Turabian StyleFarzana Gul Jan; Muhammad Hamayun; Anwar Hussain; Amjad Iqbal; Gul Jan; Sumera Afzal Khan; Hamayoon Khan; In-Jung Lee. 2019. "A promising growth promoting Meyerozyma caribbica from Solanum xanthocarpum alleviated stress in maize plants." Bioscience Reports 39, no. 10: 1.
The growth promoting activities of the isolated endophyte Aspergillus terreus from Aloe barbendsis was studied in the salt stressed Pennisetum glaucum (pearl millet). A significant (P = 0.05) increase in the root-shoot lengths, fresh and dry weights and chlorophyll content of pearl millet seedlings was noticed after colonization by A. terreus under normal conditions. At 100 mM NaCl stress and A. terreus inoculation, the growth rate of pearl millet seedlings were significantly (P = 0.05) inhibited. Furthermore, the IAA production, relative water content (RWC), chlorophyll, soluble sugar, phenol and flavonoid contents were significantly decreased, whereas proline content and lipid peroxidation were increased. On the contrary, pearl millet seedlings inoculated with A. terreus retained significantly (P = 0.05) higher amounts of RWC, chlorophyll, soluble sugar, phenol and flavonoid contents under 100 mM salt stress. The higher IAA production in A. terreus associated seedlings rescued the plant growth and development under salt stress. Moreover, the LC MS/MS analysis of A. terreus cultural filtrate revealed the presence of quinic acid, ellagic acid, calycosin, wogonin, feruloylquinic acid, caffeic acid phenylethyl ester, D-glucoside, myricetin, propoxyphene and aminoflunitrazepam. The results of the study conclude that innoculation of A. terreus improves the NaCl tolerance in pearl millet by ameliorating the physicochemical attributes of the host plants.
Faiza Khushdil; Farzana Gul Jan; Gul Jan; Muhammad Hamayun; Amjad Iqbal; Anwar Hussain; Nusrat Bibi. Salt stress alleviation in Pennisetum glaucum through secondary metabolites modulation by Aspergillus terreus. Plant Physiology and Biochemistry 2019, 144, 127 -134.
AMA StyleFaiza Khushdil, Farzana Gul Jan, Gul Jan, Muhammad Hamayun, Amjad Iqbal, Anwar Hussain, Nusrat Bibi. Salt stress alleviation in Pennisetum glaucum through secondary metabolites modulation by Aspergillus terreus. Plant Physiology and Biochemistry. 2019; 144 ():127-134.
Chicago/Turabian StyleFaiza Khushdil; Farzana Gul Jan; Gul Jan; Muhammad Hamayun; Amjad Iqbal; Anwar Hussain; Nusrat Bibi. 2019. "Salt stress alleviation in Pennisetum glaucum through secondary metabolites modulation by Aspergillus terreus." Plant Physiology and Biochemistry 144, no. : 127-134.
Crop protection against phyto-pathogens has become a global challenge that can be tackled efficiently through natural resources, including endophytic fungi. Endophytes serve as a reservoir for the vast array of potent bioactive metabolites. We investigated the antioxidant and antibacterial potency of endophytes from the roots of Solanum surattense. The non-polar fraction of the cultural filtrate from the isolated strains was tried for antibacterial potency through agar plate diffusion assay. Among the isolated strains, Penicillium roqueforti (CGF-1) and Trichoderma reesei (CGF-11) had broad-spectrum antibacterial activity against phyto-pathogenic bacteria (Xanthomonas oryzae, Pseudomonas syringae, Agrobacterium tumefaciens, and Ralstonia solanacearum). The extracts of CGF-1 and CGF-11 achieved the best result against A. tumefaciens. Similarly, qualitative analysis of the ethyl acetate extracts P. roqueforti and T. reesei exposed the occurrence of alkaloids, flavonoids, phenols, steroids, and tannins. HPLC analysis also confirmed the presence ferulic acid, cinnamic acid, quercetin, and rutin in the non-polar fraction of the cultural filtrate from the isolated strains. The results conclude that P. roqueforti and T. reesei can play an active role against the plant pathogens by secreting the bioactive compounds to protect host plant. Furthermore, the antibacterial and antioxidant potential of the P. roqueforti and T. reesei suggests its use in agriculture and pharmaceutical industry.
Muhammad Ikram; Niaz Ali; Gul Jan; Muhammad Hamayun; Farzana Gul Jan; Amjad Iqbal. Novel antimicrobial and antioxidative activity by endophytic Penicillium roqueforti and Trichoderma reesei isolated from Solanum surattense. Acta Physiologiae Plantarum 2019, 41, 164 .
AMA StyleMuhammad Ikram, Niaz Ali, Gul Jan, Muhammad Hamayun, Farzana Gul Jan, Amjad Iqbal. Novel antimicrobial and antioxidative activity by endophytic Penicillium roqueforti and Trichoderma reesei isolated from Solanum surattense. Acta Physiologiae Plantarum. 2019; 41 (9):164.
Chicago/Turabian StyleMuhammad Ikram; Niaz Ali; Gul Jan; Muhammad Hamayun; Farzana Gul Jan; Amjad Iqbal. 2019. "Novel antimicrobial and antioxidative activity by endophytic Penicillium roqueforti and Trichoderma reesei isolated from Solanum surattense." Acta Physiologiae Plantarum 41, no. 9: 164.