Due to the growing environmental and geopolitical challenges nowadays, which are causing supply chain complications, industry and society are facing significant new objections. As a complement and extension to the technology-driven premises of Industry 4.0, the value-driven Industry 5.0 focuses on society and the environment. Human centricity, sustainability, and resilience should become a more integral part of both industrial and societal revolutions. One of the enabler technologies for both is the Digital Twin (DT). In order to make DTs intelligent, they must become active, online, goal-seeking, and anticipatory. To meet these requirements, the characteristics of Multi-Agent Systems (MASs) can be employed. This paper contributes to the bilateral emergence of the two industrial paradigms and establishes an approach for the provision of Intelligent Digital Twins (IDTs) within the Internet of Digital Twins (IoDT). Initially, a DT reference model aligned with already established Industry 4.0 reference models enriched with the goals of Industry 5.0 is developed, followed by an outline of how IDTs can be realized with the characteristics of MAS. The work is substantiated by an architectural design for IDTs choreographing marketplace-oriented production processes with a subsequent prototypical implementation, followed by a proof of concept.

One of the main topics within research activities is the management of research data. Large amounts of data acquired by heterogeneous scientific devices, sensor systems, measuring equipment, and experimental setups have to be processed and ideally be managed by Findable, Accessible, Interoperable, and Reusable (FAIR) data management approaches in order to preserve their intrinsic value to researchers throughout the entire data lifecycle. The symbiosis of heterogeneous measuring devices, FAIR principles, and digital twin technologies is considered to be ideally suited to realize the foundation of reliable, sustainable, and open research data management. This paper contributes a novel architectural approach for gathering and managing research data aligned with the FAIR principles. A reference implementation as well as a subsequent proof of concept is given, leveraging the utilization of digital twins to overcome common data management issues at equipment-intense research institutes. To facilitate implementation, a top-level knowledge graph has been developed to convey metadata from research devices along with the produced data. In addition, a reactive digital twin implementation of a specific measurement device was devised to facilitate reconfigurability and minimized design effort.

The growing complexity and scale of Cyber–Physical Systems (CPSs) have led to an increasing need for the holistic orchestration of multiple Digital Twins (DTs). Therefore, an extension to the UniTwin framework is introduced within this paper. UniTwin is a containerized, cloud-native DT framework. This extension enables the hierarchical aggregation of DTs across various abstraction levels. Traditional DT frameworks often lack mechanisms for dynamic composition at the level of entire systems. This is essential for modeling distributed systems in heterogeneous environments. UniTwin addresses this gap by grouping DTs into composite entities with an aggregation mechanism. The aggregation mechanism is demonstrated in a smart manufacturing case study, which covers the orchestration of a production line for personalized shopping cart chips. It uses modular DTs provided for each device within the production line. A System-Aggregated Digital Twin (S-ADT) is used to orchestrate the individual DTs, mapping the devices in the production line. Therefore, the production line adapts and reconfigures according to user-defined parameters. This validates the flexibility and practicality of the aggregation mechanism. This work contributes an aggregation mechanism for the UniTwin framework, paving the way for adaptable DTs for complex CPSs in domains like smart manufacturing, logistics, and infrastructure.

Automated and comprehensive processing of hyperspectral image data is increasingly important in academic research and medical technology. This study presents an automated processing pipeline that integrates hyperspectral image acquisition, analysis, multimodal fusion, and centralized data management to improve the interpretability of spectral information for biological tissue analysis. The pipeline supports modular hyperspectral data processing, fusion of complementary wavelength ranges, and scalable data storage, and was implemented in Python 3.13.3. The pipeline was evaluated using hyperspectral imaging data acquired from a coronal mouse brain section. Clustering-based analysis and spectral correlation metrics were applied to assess the impact of multimodal data fusion on spectral representation. Clustering of individual modalities yielded silhouette coefficients of 0.5879 for near-infrared data, 0.6020 for mid-infrared data, and 0.6715 for RGB data. Multimodal fusion reduced the silhouette coefficient to 0.5420 and enabled the identification of anatomical structures that were not distinguishable in any single modality. High spectral correlation coefficients exceeding 0.98 confirmed that spectral fidelity was preserved during fusion. These results demonstrate that automated multimodal hyperspectral data fusion can enhance the interpretability of biological tissue despite reduced clustering compactness. The proposed pipeline provides a structured framework for preclinical hyperspectral imaging workflows and supports exploratory biological analysis in medical imaging contexts.

In data-intensive research, reliable management of research data is a major challenge. In the field of Mass Spectrometry Imaging, vast amounts of data are being acquired from mostly proprietary data sources. Consequently, hindering seamless data integration into Research Data Management systems. Without a data repository, the continuous generation of scientific knowledge and innovative research based on existing information is limited. Moreover, to maintain the value of data to researchers throughout and beyond its lifecycle, FAIR principles for reliable data management approaches must be applied. To enable the required data transmission, the Digital Twin paradigm can be considered a reliable solution. The conceptual implementation of a heterogeneous mass spectrometer generating hyperspectral images leverages the Digital Twin to overcome common data management problems in data-intensive research.