OBJECTIVES: The objective of this study was to investigate the potential protective effect of Nigella sativa oil (NSO) against cis-diamminedichloroplatinum or cisplatin (CDDP)-induced ototoxicity. MATERIALS and METHODS: Twenty-four Wistar albino rats were randomly and equally divided into four groups. Groups 1 and 2 were given a total of 15 mg/kg CDDP intraperitoneally, which was divided equally into three doses on days 1, 3, and 5. Group 2 was treated via gavage feeding with 15 ml NSO that was divided into five doses on days 1, 3, 5, 7, and 9. Groups 3 and 4 received only 15 ml of NSO and 15 ml of 0.9% saline solution, respectively, which were orally administered and divided into five doses on days 1, 3, 5, 7, and 9. Baseline high-frequency (8, 12, 16, and 32 kHz) auditory brainstem response (ABR) measurements were collected in all the groups before the medical administrations and were repeated on the 14th day before sacrifice. Afterward, a histopathological evaluation of the cochlea was performed. RESULTS: There was a significant difference in the histopathological changes between group 1 and the other groups (p<0.01). Changes in the spiral ganglion cells, the stria vascularis, and the external ciliated cells were significantly different between groups 1 and 2 (p=0.019, 0.039, and 0.045, respectively). The ABR results revealed significant differences in the 16 and 32 kHz measurements between groups 1 and 2 (p=0.013 and p<0.01, respectively). CONCLUSION: According to the results, NSO may have a protective effect on cochlear function against the disruptive effects of CDDP in rats. Cite this article as: Kökten N, Eğilmez OK, Erinç M, Doğan Ekici AI, Şerifler S, Yeşilada E, et al. The Protective Effect of Nigella sativa Oil against Experimentally Induced Cisplatin Ototoxicity: An Animal Study. J Int Adv Otol 2020; 16(3): 346-52.

The quality and reliability of health-related content on YouTube remain a growing concern. This study aimed to evaluate tonsillectomy-related YouTube videos using a multi-method framework that combines human expert review, large language model (ChatGPT-4) analysis, and transcript readability assessment. A total of 76 English-language YouTube videos were assessed. Two otolaryngologists independently rated video quality using the DISCERN instrument and JAMA benchmarks. Corrected transcripts were evaluated by ChatGPT-4 (May 2024 version) for accuracy and completeness. Spearman correlations and regression analyses were used to explore associations between human and AI evaluations. Videos were also categorized as transcript-heavy or visually rich to examine the effect of visual presentation. Professional videos consistently outperformed patient-generated content in quality metrics. ChatGPT-4 accuracy scores showed a strong correlation with JAMA ratings (ρ = 0.56), while completeness was strongly associated with DISCERN scores (ρ = 0.72). Visually rich videos demonstrated significantly higher AI accuracy than transcript-heavy videos (Cohen’s d = 0.600, p = 0.030), suggesting that visual context may enhance transcript-based interpretation. However, the average transcript readability (FKGL = 8.38) exceeded the recommended level for patient education. Tonsillectomy-related YouTube content varies widely in quality. Human–AI alignment supports the use of large language models for preliminary content screening. Visually enriched content may improve AI interpretability, while readability concerns highlight the need for more accessible educational resources. Multimodal evaluation and design should be prioritized in future digital health content.

The aim of this experimental study was to investigate the histopathologic effect of Nigella Sativa oil (NSO) on cisplatin (Cis) induced oral mucositis (OM) in rats. Twenty-four rats were divided into four equal groups. The animals in Group 1 and Group 2 were given 5 mg/kg intraperitoneal (ip) Cis systemically on the 1st, 3rd and 5th days of the study. Additionally, 15 mL NSO were given to the rats in Group 2, with gavage feeding on days 1, 3, 5, 7, and 9. The animals in Group 3 were given per oral 15 ml NSO on days 1, 3, 5, 7 and 9. As the control group, Group 4 received a total of 15 mL 0.9% saline solution divided into 5 doses on days 1, 3, 5, 7 and 9 by oral gavage. On the 14th day, animals were euthanized and buccal mucosa from both sides, including submucosal tissues, were excised and taken to histopathological examination. The mean mucosal thicknesses of the groups were 224.58 μm, 276.1 μm, 323.33 μm, and 331.33 μm, respectively for Groups 1, 2, 3, and 4 (p0.05). According to the results of this study, NSO, for which anti-inflammatory and antioxidant properties have been shown in previous studies, may also be effective in preventing Cis-induced OM.

Objective: The pathophysiology of sudden hearing loss remains poorly understood. Research aimed at elucidating its etiology is crucial for the early diagnosis and treatment of patients. This study aimed to clarify the role of inflammation in the etiology of sudden hearing loss, which remains poorly understood, by analyzing the C-reactive protein-to-albumin ratio (CAR), systemic immune-inflammation index (SII), prognostic nutritional index (PNI), and neutrophil-to-lymphocyte ratio (NLR) values associated with inflammatory processes. Materials and Methods: A chart review of patients with idiopathic sudden hearing loss admitted to the hospital between January 2019 and December 2020 was conducted. The study included 35 patients with this condition and 30 healthy individuals for comparison. The significance level was set at p<0.05. Results: The mean age of the patient group was 48.26, while the mean age of the healthy group was 44.04. In the patient group, 15 were female, and 20 were male. Significant differences were observed in NLR, CAR, and SII values between the recovery and non-recovery idiopathic sudden sensorineural hearing loss (ISSHL) groups. The non-recovery group exhibited higher NLR (3.40±0.20), CAR (0.70±0.09), and SII (687.9±76.8) values (with p-values of 0.021, 0.026, and 0.038, respectively). In the ISSHL group, NLR exhibited approximately 87% sensitivity when above 3.41, while SII demonstrated approximately 69.6% sensitivity when exceeding 650.39. (p=0.038 and p=0.037, respectively). Conclusion: The levels of NLR and SII index might help predict early outcomes in sudden hearing loss of unknown cause.

Objective: This study aims to investigate the contribution of monocyte/high-density lipoprotein (HDL) ratio (MHR) and monocyte/lymphocyte ratio (MLR) to the inflammatory process and the severity and prognosis of the disease in patients with Bell’s palsy. Materials and methods: The study was designed retrospectively by analyzing our electronic database. A study group consisted of 48 patients who were referred to our clinic with Bell’s palsy between January 2018 and June 2020. The control group consisted of 45 healthy individuals. Monocyte, HDL, neutrophil, lymphocyte, and platelet values were recorded. The hematological parameters obtained from the blood tests of the patients in the study group at the time of admission were statistically compared with the values in the control group. Radiologic images were also collected. Results: The MHR value of the study group was 12.85±1.02, while the MHR value of the control group was 12.29±1.33, and it showed a statistically significant difference (p=0.027). However, no statistically significant difference between the groups was found in other parameters, including MLR, neutrophil/lymphocyte ratio (NLR), and platelet/lymphocyte ratio (PLR). A positive correlation was found between the MHR value and the House-Brackmann stage. The NLR value of the patients who showed contrast enhancement in facial nerves on MRI was found to be statistically significant compared to those without contrast enhancement. Conclusion: High MHR values in patients with Bell’s palsy support the role of inflammatory and ischemic processes in etiopathogenesis. Further studies are needed to confirm our results in a multi-center manner with larger patient populations.