Background/Objectives: Dioscorea batatas Decne (yam), which contains various bioactive compounds, has been utilized in the cosmetics industry, while most of the peel of D. batatas (DBP) is discarded without further use. Recent studies have shown that DBP contains higher levels of bioactive substances than the rhizome flesh. The aim of this study was to evaluate the skin biological activities of DBP extracts obtained using 70% ethanol (70% EtOH DBP), 95% ethanol (95% EtOH DBP), and ethyl acetate (EA DBP), with particular attention to their antioxidant-associated protective effects. Methods: Skin-related bioactivities of DBP extracts prepared using ultrasonic extraction were evaluated using in vitro tyrosinase and matrix metalloproteinase-1 (MMP-1) assays, alpha-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis in B16F10 cells, ultraviolet B (UVB)-irradiated HaCaT viability assays, and Western blot analysis of pro-collagen type I alpha 1(Pro-COL1A1) and MMP-1 in HDF cells. In addition, the ABTS and DPPH radical scavenging activities of DBP extracts and representative DBP derivatives were assessed. Results: DBP extracts inhibited tyrosinase activity in vitro and reduced melanogenesis in B16F10 cells. DBP extracts also protected skin cells from UVB by increasing the viability of UVB-irradiated HaCaT cells. In UVB-irradiated HDF cells, DBP extracts restored Pro-COL1A1 expression and suppressed MMP-1 levels. Additionally, DBP extracts inhibited MMP-1 activity in a concentration-dependent manner. The DBP extracts themselves exhibited ABTS and DPPH radical scavenging activities, with EA DBP showing the highest vitamin C equivalent antioxidant capacity among the tested extracts. Representative DBP-derived phenanthrene compounds also showed radical scavenging activities, supporting the antioxidant potential of peel-derived phytochemicals. Conclusions: These findings indicate that DBP extracts possess skin-whitening and anti-photoaging effects and suggest that these protective activities may be associated with the antioxidant potential of both DBP extracts and DBP derivatives.

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