Tribulus terrestris L. is a herb renowned for its abundance of saponins, flavonoids, and alkaloids, which are utilized in treating various health conditions. Protodioscin functions by enhancing the conversion of testosterone into potent dihydrotestosterone, stimulating an increase in libido, red blood cell production from the bone marrow, and muscle development. Contemporary ultrasound-assisted-extraction (UAE) process employing green extraction solvents was selected to design the required separation system. The experimental plan was developed based on the independent operating variables - extraction time, operating temperature and solvent system composition in order to determine the influence of defined parameters and their interactions on the extraction yield and the presence of protodioscin. The 3D-RSM approach was introduced to determine the optimal values of studied independent variables in the area of maximal extraction yield. UAE process performed at optimal operating conditions generated maximal extraction yield (31 %, w/w) and protodioscin content of 5.9 mg/g dry plant matrix. Experimental data was used to develop an ANN for the defined extraction system using the operating variables values as the input matrix and observed yield as target matrix. Successfully designed and trained ANN generated high correlation (r = 0.9992) between observed data and predictive model' outputs, and MSE value of 0.29107.

The goal of this work is isolation of tomato seed oil from the tomato waste (skin and seed) from tomato processing industry by utilization of non-conventional, green solvent technology. In the frames of experimental research, the influence of working parameters (temperature, pressure and extraction time) on the total yield of isolated tomato seed oil. Dynamic method for determination of solubility parameter in a supercritical CO2 has been used to determine the solubility of tomato seed oil in the supercritical CO2. Obtained results regarding the influence of operating parameters on total yield of extracted tomato seed oil are presented graphically. The influence of the supercritical fluid’ density on the tomato seed oil’ solubility was determined based on those results. The chemical composition of isolated tomato seed oil was determined by application of Gas Chromatography.