A high-performance liquid chromatographic method with diode array detection has been developed and optimized for the separation of five calcium channel blockers belonging to the 1,4-dihydropyridine subgroup (nifedipine and related drugs). The possibility of the simultaneous drug analysis allows a decrease of time during the assay as well as a saving of reagents and solvents. In this work, the effect of four experimental parameters (organic modifier percentage, pH value, concentration of the buffer in the mobile phase, and column temperature) on the chromatographic resolution are investigated by experimental design in order to optimize the chromatographic separation of five 1,4-dihydropyridines (amlodipine, nitrendipine, felodipine, lacidipine, and lercanidipine). Fractional factorial design, central composite design, and finally the Multisimplex program are used to establish the optimal conditions in terms of resolution and minimum analysis time. Optimal separation of the five compounds under study is achieved in less than 12 min using a Sulpecosil LCABZ+ Plus C18 column, a composition of mobile phase of acetonitrile—10mM acetic acid acetate buffer pH 5 (72:28, v/v) at a flow rate of 1 mL/min, a column temperature of 30°C ± 0.1°C, and a detection wavelength of 238 nm.

Background: Growing concerns about health and sustainability are accelerating the need to transform food systems to promote well-being and reduce environmental impacts. Despite increasing policy efforts, evidence remains limited on self-perceived knowledge, reported practices, intentions, and perceived barriers related to healthy and sustainable eating among European households with children. Establishing healthy eating habits during childhood is essential for long-term health, with parents playing a pivotal role in shaping these behaviors. Understanding the factors and barriers related to food choices is essential for informing interventions that support healthier and more sustainable dietary practices. Methods: This study, conducted in 2023 as part of the European TITAN project, explored parental perceptions and practices related to healthy and sustainable eating in Spain, Poland, and Finland. A structured questionnaire was completed by 240 parents of children aged 6–12 in each country using quota sampling based on national income tertiles. Results: Although households reported high self-perceived knowledge, patterns consistent with a possible discrepancy between self-perceived knowledge and reported food choices were observed. Finnish households, despite showing the highest recognition of predefined healthy and sustainable eating concepts, reported lower self-reported adherence to healthy and sustainable practices. Reducing meat consumption and choosing sustainably sourced seafood were among the least valued actions across all countries. Cost was the most frequently reported barrier, especially in Finland (60%), alongside greater difficulty in forming new habits and resisting less healthy options. Overall, cost and behavioral constraints were among the most frequently reported barriers to healthier and more sustainable dietary practices. Conclusions: These findings highlight the need for culturally tailored strategies to support households in transitioning toward healthier and more sustainable dietary behaviors. Addressing perceived barriers and food-choice constraints may be important for supporting healthier and more sustainable dietary practices at the household level.