MicroRNA (miRNA) profile-based point-of-care (POC) diagnostic methods have attracted considerable attention. In our laboratory, singleplex miRNA detection on a power-free poly(dimethylsiloxane) (PDMS) microfluidic chip with laminar flow-assisted dendritic amplification (LFDA) has been developed. In this study, to obtain the miRNA profile and to improve the reliability of the diagnosis, multiplex miRNA detection on the same system is demonstrated without compromising any advantages of the singleplex miRNA detection. The limit of detection (LOD) was at the femto- to picomolar level and the assay time was 20 min. The sensitivity, rapidity, and portability of the microfluidic chip are adequate for POC diagnosis.

We investigated the effects of particle size, temperature, electrolyte concentration, and probe length on the non-crosslinked aggregation of DNA-modified GNPs.

Joule heating and negative dielectrophoresis, caused by AC electric fields in a microchannel, have been employed for cloud point extraction of phospholipid as a model of membrane-associated biomolecules.