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Dr. Luisa Gouveia
Bioenergy Unit, LNEG - National Laboratory of Energy and Geology, I.P., 1649-038 Lisbon, Portugal

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0 Food
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microalgae
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biofertilizers
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Journal article
Published: 05 May 2021 in Environmental Advances
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Pig farming generates highly polluting wastewaters which entail serious environmental issues when not adequately managed. Microalgae systems can be promising for cost, energy and environment-efficient treatment of piggery wastewater (PWW). Aside from clean water, the produced biomass can be used as biostimulants and biopesticides contributing to a more sustainable agriculture. Three microalgae (Tetradesmus obliquus, Chlorella protothecoides, Chlorella vulgaris) and one cyanobacterium (Synechocystis sp.) were selected after a preliminary screening in diluted wastewater (1:20) to treat PWW. The nutrient removals were 62-79% for COD (chemical oxygen demand), 84-92% for TKN (total Kjeldahl nitrogen), 79-92% for NH4+ and over 96% for PO43−. T. obliquus and C. protothecoides were the most efficient ones. After treating PWW, the produced biomass, at 0.5 g L−1, was assessed as a biostimulant for seed germination, root/shoot growth, and pigment content for tomato, watercress, cucumber, soybean, wheat, and barley seeds. We observed an overall increase on germination index (GI) of microalgae-treated seeds, owing to the development of longer roots, especially in T. obliquus and C. vulgaris treatments. The microalgae treatments were especially effective in cucumber seeds (75-138% GI increase). The biopesticide activity against Fusarium oxysporum was also evaluated at 1, 2.5 and 5 g L−1 of microalgae culture. Except for Synechocystis sp., all the microalgae tested inhibited the fungus growth, with T. obliquus and C. vulgaris achieving inhibitions above 40% for all concentrations.

ACS Style

Alice Ferreira; Lusine Melkonyan; Sofia Carapinha; Belina Ribeiro; Daniel Figueiredo; Gayane Avetisova; Luisa Gouveia. Biostimulant and biopesticide potential of microalgae growing in piggery wastewater. Environmental Advances 2021, 4, 100062 .

AMA Style

Alice Ferreira, Lusine Melkonyan, Sofia Carapinha, Belina Ribeiro, Daniel Figueiredo, Gayane Avetisova, Luisa Gouveia. Biostimulant and biopesticide potential of microalgae growing in piggery wastewater. Environmental Advances. 2021; 4 ():100062.

Chicago/Turabian Style

Alice Ferreira; Lusine Melkonyan; Sofia Carapinha; Belina Ribeiro; Daniel Figueiredo; Gayane Avetisova; Luisa Gouveia. 2021. "Biostimulant and biopesticide potential of microalgae growing in piggery wastewater." Environmental Advances 4, no. : 100062.

Journal article
Published: 15 July 2020 in Algal Research
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Microalgae have been widely used as a source of functional ingredients such as pigments, antioxidants, vitamins, and omega-3 polyunsaturated fatty acids. They also represent a promising alternative source of protein. The objective of this study was to evaluate the impact of the addition of two green microalgae species (Nannochloropsis gaditana L2 and Chlamydomonas sp. EL5) on the techno-functional and nutritional properties of gluten-free bread. Microalgae biomass was added in the amounts of 1.0 and 3.0 g/100 g of flour. The behavior of the dough during the mixing as well as the physicochemical properties of the prepared breads were investigated. Gluten-free bread with N. gaditana L2 and Chlamydomonas sp. EL5 presented significantly higher protein and higher levels of lipids and ash, compared with the control bread. The incorporation of 3% microalgae biomass revealed a 100% increase in iron and calcium contents. The fatty acid profile of supplemented bread changed in a species-specific manner with a particular increase in linolenic acid (18:3 ω3) and a decrease in ω3/ω6 ratio. Besides, due to its original biochemical composition, mainly the highly protein content, microalgae incorporation was found to bring an overall structuring effect on the gluten-free bread texture. However, the dough mixing properties were not affected significantly by microalgae addition. A significant change in color was recorded in doughs, breads, crusts and crumbs. This was caused by the presence of pigment in microalgae biomass, which turned into more intense green-yellow tonalities. A sensory analysis revealed that the supplemented breads scored highest for nearly all the sensory parameters with the 3% N. gaditana L2 bread as the preferred one in terms of global appreciation. This innovative approach gives new insights of the possibility of improving gluten-free products, structurally and nutritionally, using only microalgae as a natural and a sustainable food ingredient.

ACS Style

Sheyma Khemiri; Nadia Khelifi; Maria Cristiana Nunes; Alice Ferreira; Luisa Gouveia; Issam Smaali; Anabela Raymundo. Microalgae biomass as an additional ingredient of gluten-free bread: Dough rheology, texture quality and nutritional properties. Algal Research 2020, 50, 101998 .

AMA Style

Sheyma Khemiri, Nadia Khelifi, Maria Cristiana Nunes, Alice Ferreira, Luisa Gouveia, Issam Smaali, Anabela Raymundo. Microalgae biomass as an additional ingredient of gluten-free bread: Dough rheology, texture quality and nutritional properties. Algal Research. 2020; 50 ():101998.

Chicago/Turabian Style

Sheyma Khemiri; Nadia Khelifi; Maria Cristiana Nunes; Alice Ferreira; Luisa Gouveia; Issam Smaali; Anabela Raymundo. 2020. "Microalgae biomass as an additional ingredient of gluten-free bread: Dough rheology, texture quality and nutritional properties." Algal Research 50, no. : 101998.

Journal article
Published: 04 February 2020 in Molecules
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Microalgae are microorganisms with the capacity to contribute to the sustainable and healthy food production, in addition to wastewater treatment. The subject of this work was to determine the potential of Scenedesmus obliquus microalga grown in brewery wastewater to act as a plant biostimulant. The germination index of watercress seeds, as well as the auxin-like activity in mung bean and cucumber, and in the cytokinin-like activity in cucumber bioassays were used to evaluate the biostimulant potential. Several biomass processes were studied, such as centrifugation, ultrasonication and enzymatic hydrolysis, as well as the final concentration of microalgal extracts to determine their influence in the biostimulant activity of the Scenedesmus biomass. The results showed an increase of 40% on the germination index when using the biomass at 0.1 g/L, without any pre-treatment. For auxin-like activity, the best results (up to 60% with respect to control) were obtained at 0.5 g/L of biomass extract, after a combination of cell disruption, enzymatic hydrolysis and centrifugation. For cytokinin-like activity, the best results (up to 187.5% with respect to control) were achieved without cell disruption, after enzymatic hydrolysis and centrifugation at a biomass extract concentration of 2 g/L.

ACS Style

Elvira Navarro-López; Angela Ruíz-Nieto; Alice Ferreira; F. Gabriel Acién; Luisa Gouveia. Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater. Molecules 2020, 25, 664 .

AMA Style

Elvira Navarro-López, Angela Ruíz-Nieto, Alice Ferreira, F. Gabriel Acién, Luisa Gouveia. Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater. Molecules. 2020; 25 (3):664.

Chicago/Turabian Style

Elvira Navarro-López; Angela Ruíz-Nieto; Alice Ferreira; F. Gabriel Acién; Luisa Gouveia. 2020. "Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater." Molecules 25, no. 3: 664.

Journal article
Published: 31 January 2020 in Coatings
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Six microalgae strains were screened according to their biomass productivity and polymer synthesis, showing biomass productivity between 0.14 and 0.68 g/(L·d) for a 21-day growth period. Extracellular biopolymers from the spent culture media of Nostoc sp. (No), Synechocystis sp. (Sy), and Porphyridium purpureum (Pp) was obtained, and the yields of the clean biopolymer were 323, 204, and 83 mg/L, respectively. The crude biopolymer was cleaned up using a solid-phase extraction technique. The emulsification index E24 values for the clean biopolymer were 77.5%, 68.8%, and 73.3% at 0.323, 0.083, and 0.204 mg/mL, respectively. The clean biopolymer of the No strain showed the highest fungal growth inhibition against Fusarium verticillioides (70.2%) and Fusarium sp. (61.4%) at 2.24 mg/mL. In general, transparent and flexible biofilms were prepared using biopolymers of No and Pp. The microstructural analysis revealed the presence of pores and cracks in the biofilms, and the average roughness Ra values are 68.6 and 86.4 nm for No and Pp, respectively, and the root mean square roughness Rq values are 86.2 and 107.2 nm for No and Pp, respectively.

ACS Style

Mónica Morales-Jiménez; Luisa Gouveia; Jorge Yáñez-Fernández; Roberto Castro-Muñoz; Blanca Estela Barragán-Huerta. Production, Preparation and Characterization of Microalgae-Based Biopolymer as a Potential Bioactive Film. Coatings 2020, 10, 120 .

AMA Style

Mónica Morales-Jiménez, Luisa Gouveia, Jorge Yáñez-Fernández, Roberto Castro-Muñoz, Blanca Estela Barragán-Huerta. Production, Preparation and Characterization of Microalgae-Based Biopolymer as a Potential Bioactive Film. Coatings. 2020; 10 (2):120.

Chicago/Turabian Style

Mónica Morales-Jiménez; Luisa Gouveia; Jorge Yáñez-Fernández; Roberto Castro-Muñoz; Blanca Estela Barragán-Huerta. 2020. "Production, Preparation and Characterization of Microalgae-Based Biopolymer as a Potential Bioactive Film." Coatings 10, no. 2: 120.

Journal article
Published: 03 September 2019 in Molecules
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Commercial production of microalgal biomass for food and feed is a recent worldwide trend. Although it is common to publish nutritional data for microalgae grown at the lab-scale, data about industrial strains cultivated in an industrial setting are scarce in the literature. Thus, here we present the nutritional composition and a microbiological and toxicological evaluation of Tetraselmis sp. CTP4 biomass, cultivated in 100-m3 photobioreactors at an industrial production facility (AlgaFarm). This microalga contained high amounts of protein (31.2 g/100 g), dietary fibres (24.6 g/100 g), digestible carbohydrates (18.1 g/100 g) and ashes (15.2 g/100 g), but low lipid content (7.04 g/100 g). The biomass displayed a balanced amount of essential amino acids, n-3 polyunsaturated fatty acids, and starch-like polysaccharides. Significant levels of chlorophyll (3.5 g/100 g), carotenoids (0.61 g/100 g), and vitamins (e.g., 79.2 mg ascorbic acid /100 g) were also found in the biomass. Conversely, pathogenic bacteria, heavy metals, cyanotoxins, mycotoxins, polycyclic aromatic hydrocarbons, and pesticides were absent. The biomass showed moderate antioxidant activity in several in vitro assays. Taken together, as the biomass produced has a balanced biochemical composition of macronutrients and (pro-)vitamins, lacking any toxic contaminants, these results suggest that this strain can be used for nutritional applications.

ACS Style

Hugo Pereira; Joana Silva; Tamára Santos; Katkam N. Gangadhar; Ana Raposo; Cláudia Nunes; Manuel A. Coimbra; Luísa Gouveia; Luísa Barreira; João Varela. Nutritional Potential and Toxicological Evaluation of Tetraselmis sp. CTP4 Microalgal Biomass Produced in Industrial Photobioreactors. Molecules 2019, 24, 3192 .

AMA Style

Hugo Pereira, Joana Silva, Tamára Santos, Katkam N. Gangadhar, Ana Raposo, Cláudia Nunes, Manuel A. Coimbra, Luísa Gouveia, Luísa Barreira, João Varela. Nutritional Potential and Toxicological Evaluation of Tetraselmis sp. CTP4 Microalgal Biomass Produced in Industrial Photobioreactors. Molecules. 2019; 24 (17):3192.

Chicago/Turabian Style

Hugo Pereira; Joana Silva; Tamára Santos; Katkam N. Gangadhar; Ana Raposo; Cláudia Nunes; Manuel A. Coimbra; Luísa Gouveia; Luísa Barreira; João Varela. 2019. "Nutritional Potential and Toxicological Evaluation of Tetraselmis sp. CTP4 Microalgal Biomass Produced in Industrial Photobioreactors." Molecules 24, no. 17: 3192.

In the field
Published: 02 July 2019 in Biofuels, Bioproducts and Biorefining
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The circular bioeconomy concept relies on the exploitation of wastes as a feedstock of different biotechnological processes to obtain, as much as possible, a huge spectrum of biochemical components through a biorefinery platform. This work deals with the treatment of brewery effluent through the cultivation of Scenedesmus obliquus microalga and the use of the biomass in a complex biorefinery. The treatment proved efficient in the removal of nutrients (N, P and COD removals of 88, 30 and 71% respectively). Several compounds and products were obtained from the biomass, such as: (a) phenols (0.249–1.016 mg GAE mL−1) and flavonoids (0.05–0.167 mg CE mL−1) by subcritical water extraction (SWE) at 120 °C, the extraction efficiency being five times higher at 200 °C; (b) biohydrogen by dark fermentation (67.1 mL H2 gVS−1); (c) bio‐oil (64%), biochar (30%) and biogas (6%) by pyrolysis; and (d) enhanced capacity of germination/growth of wheat and barley seeds by S. obliquus culture and biomass (pellet, after centrifugation); better results were obtained with the biomass cultivated in brewery effluent (when compared with synthetic medium), and the biomass pellet was better than the whole culture; barley seeds treated with the pellet from the brewery effluent had the highest germination index (GI) of 85 compared with the control (tap water) GI of 35. The innovative study emphasis was on reducing microalgae production costs, providing environmental benefits in a biorefinery‐based S. obliquus platform. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

ACS Style

Alice Ferreira; Belina Ribeiro; Ana Filipa Ferreira; Marileide L. A. Tavares; Jelena Vladic; Senka Vidović; Marko Cvetkovic; Lusine Melkonyan; Gayane Avetisova; Vigen Goginyan; Luísa Gouveia. Scenedesmus obliquusmicroalga‐based biorefinery – from brewery effluent to bioactive compounds, biofuels and biofertilizers – aiming at a circular bioeconomy. Biofuels, Bioproducts and Biorefining 2019, 13, 1169 -1186.

AMA Style

Alice Ferreira, Belina Ribeiro, Ana Filipa Ferreira, Marileide L. A. Tavares, Jelena Vladic, Senka Vidović, Marko Cvetkovic, Lusine Melkonyan, Gayane Avetisova, Vigen Goginyan, Luísa Gouveia. Scenedesmus obliquusmicroalga‐based biorefinery – from brewery effluent to bioactive compounds, biofuels and biofertilizers – aiming at a circular bioeconomy. Biofuels, Bioproducts and Biorefining. 2019; 13 (5):1169-1186.

Chicago/Turabian Style

Alice Ferreira; Belina Ribeiro; Ana Filipa Ferreira; Marileide L. A. Tavares; Jelena Vladic; Senka Vidović; Marko Cvetkovic; Lusine Melkonyan; Gayane Avetisova; Vigen Goginyan; Luísa Gouveia. 2019. "Scenedesmus obliquusmicroalga‐based biorefinery – from brewery effluent to bioactive compounds, biofuels and biofertilizers – aiming at a circular bioeconomy." Biofuels, Bioproducts and Biorefining 13, no. 5: 1169-1186.

Chapter
Published: 01 January 2019 in Grand Challenges in Algae Biotechnology
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Conventional wastewater treatment (WWT) is currently based on the action of heterotrophic and nitrifying bacteria supported by mechanical aeration in a sequence of interconnected aerobic, anoxic, and anaerobic processes. It provided satisfactory levels of pollutant removal, but at the expenses of high-energy consumption and environmental impacts (high CO2 footprint, nutrient losses, and secondary pollution due to the use of chemicals). Algal-bacterial systems constitute a sustainable and cost-effective alternative WWT based in solar-driven oxygenation (mediated by photosynthesis), enhanced nutrient assimilation into the biomass (resulting from combined auto-, mixo-, and heterotrophic growth), and efficient pathogen removal (due to high pH and O2 concentrations). Moreover, microalgae-based WWT can be combined with flue gas treatment since microalgae are able to bioconvert CO2 into biomass that can be transformed efficiently into biofuels, biofertilizers, and bioplastics or allow the recovery of high-value products using subcritical water extraction. Many industries generate huge amounts of wastewater in their processes (e.g., urban, aquaculture, poultry, cattle, swine, dairy, brewery, tobacco, etc.) with high loads of organics and nutrients that need to be removed before they can be discharged safely into the environment. The whole process can be envisaged as a microalgae-based biorefinery, which is crucial for the full use and commercialization of microalgae in various areas, allowing the incorporation of a large range of products and associated industries, which brings enormous benefits for bioeconomy and emerging societies. This chapter provides the view and results from the authors concerning the treatment of several WWs, the characterization of the produced microalgae biomass, the biochemical and thermochemical conversion of the biomass, as well as the extraction of compounds toward different biofuels and bio-based products. The importance of these coupling processes into a biorefinery and bioeconomy frames will be validated by a literature review of life cycle assessment to highlight the added value from an environmental perspective.

ACS Style

Alice Ferreira; Alberto Reis; Senka Vidovic; Jelena Vladic; Spyros Gkelis; Lusine Melkonyan; Gayane Avetisova; Roberta Congestri; Gabriel Acién; Raul Muñoz; Pierre Collet; Luisa Gouveia. Combining Microalgae-Based Wastewater Treatment with Biofuel and Bio-Based Production in the Frame of a Biorefinery. Grand Challenges in Algae Biotechnology 2019, 319 -369.

AMA Style

Alice Ferreira, Alberto Reis, Senka Vidovic, Jelena Vladic, Spyros Gkelis, Lusine Melkonyan, Gayane Avetisova, Roberta Congestri, Gabriel Acién, Raul Muñoz, Pierre Collet, Luisa Gouveia. Combining Microalgae-Based Wastewater Treatment with Biofuel and Bio-Based Production in the Frame of a Biorefinery. Grand Challenges in Algae Biotechnology. 2019; ():319-369.

Chicago/Turabian Style

Alice Ferreira; Alberto Reis; Senka Vidovic; Jelena Vladic; Spyros Gkelis; Lusine Melkonyan; Gayane Avetisova; Roberta Congestri; Gabriel Acién; Raul Muñoz; Pierre Collet; Luisa Gouveia. 2019. "Combining Microalgae-Based Wastewater Treatment with Biofuel and Bio-Based Production in the Frame of a Biorefinery." Grand Challenges in Algae Biotechnology , no. : 319-369.

Evaluation study
Published: 01 August 2018 in Journal of Bioscience and Bioengineering
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In this work, hydrogen (H2) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90.3% (w/w). The H2 production from Spirogyra hydrolysate was 3.9 L H2 L−1, equivalent to 146.3 mL H2 g−1 microalga dry weight. The presence of protein (23.2 ± 0.3% w/w) and valuable pigments, such as astaxanthin (38.8% of the total pigment content), makes this microalga suitable to be used simultaneously in both food and feed applications. In a Spirogyra based biorefinery, the potential energy production and food-grade protein and pigments revenue per cubic meter of microalga culture per year was estimated on 7.4 MJ, US $412 and US $15, respectively, thereby contributing to the cost efficiency and sustainability of the whole bioconversion process.

ACS Style

Tiago Pinto; Luísa Gouveia; Joana Ortigueira; Ganesh Dattatraya Saratale; Patrícia Moura. Enhancement of fermentative hydrogen production from Spirogyra sp. by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model. Journal of Bioscience and Bioengineering 2018, 126, 226 -234.

AMA Style

Tiago Pinto, Luísa Gouveia, Joana Ortigueira, Ganesh Dattatraya Saratale, Patrícia Moura. Enhancement of fermentative hydrogen production from Spirogyra sp. by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model. Journal of Bioscience and Bioengineering. 2018; 126 (2):226-234.

Chicago/Turabian Style

Tiago Pinto; Luísa Gouveia; Joana Ortigueira; Ganesh Dattatraya Saratale; Patrícia Moura. 2018. "Enhancement of fermentative hydrogen production from Spirogyra sp. by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model." Journal of Bioscience and Bioengineering 126, no. 2: 226-234.

Journal article
Published: 01 July 2018 in Environmental Research
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The ability of microalgae to grow in nutrient-rich environments and to accumulate nutrients from wastewaters (WW) makes them attractive for the sustainable and low-cost treatment of WW. The valuable biomass produced can be further used for the generation of bioenergy, animal feed, fertilizers, and biopolymers, among others. In this study, Scenedesmus obliquus was able to remove nutrients from different wastewaters (poultry, swine and cattle breeding, brewery and dairy industries, and urban) with removal ranges of 95-100% for nitrogen, 63-99% for phosphorus and 48-70% for chemical oxygen demand. The biomass productivity using wastewaters was higher (except for poultry) than in synthetic medium (Bristol), the highest value being obtained in brewery wastewater (1025 mg/(L.day) of freeze-dried biomass). The produced biomass contained 31-53% of proteins, 12-36% of sugars and 8-23% of lipids, regardless of the type of wastewater. The potential of the produced Scenedesmus obliquus biomass for the generation of BioH through batch dark fermentation processes with Enterobacter aerogenes was evaluated. The obtained yields ranged, in mL H/g Volatile Solids (VS), from 50.1 for biomass from anaerobically digested cattle WW to 390 for swine WW, whereas the yield with biomass cultivated in Bristol medium was 57.6 mL H/g.

ACS Style

Alice Ferreira; Paula Marques; Belina Ribeiro; Paula Assemany; Henrique Vieira de Mendonça; Ana Barata; Ana Cristina Oliveira; Alberto Reis; Helena Pinheiro; Luisa Gouveia. Combining biotechnology with circular bioeconomy: From poultry, swine, cattle, brewery, dairy and urban wastewaters to biohydrogen. Environmental Research 2018, 164, 32 -38.

AMA Style

Alice Ferreira, Paula Marques, Belina Ribeiro, Paula Assemany, Henrique Vieira de Mendonça, Ana Barata, Ana Cristina Oliveira, Alberto Reis, Helena Pinheiro, Luisa Gouveia. Combining biotechnology with circular bioeconomy: From poultry, swine, cattle, brewery, dairy and urban wastewaters to biohydrogen. Environmental Research. 2018; 164 ():32-38.

Chicago/Turabian Style

Alice Ferreira; Paula Marques; Belina Ribeiro; Paula Assemany; Henrique Vieira de Mendonça; Ana Barata; Ana Cristina Oliveira; Alberto Reis; Helena Pinheiro; Luisa Gouveia. 2018. "Combining biotechnology with circular bioeconomy: From poultry, swine, cattle, brewery, dairy and urban wastewaters to biohydrogen." Environmental Research 164, no. : 32-38.

Journal article
Published: 18 June 2018 in Environmental Technology
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Wastewater biological treatment with microalgae can be an effective technology, removing nutrients and other contaminants while reducing chemical oxygen demand. This can be particularly interesting for the meat producing industry which produces large volumes of wastewater from the slaughtering of animals and cleaning of their facilities. The main purpose of this research was the treatment of poultry wastewater using Scenedesmus obliquus in an economical and environmentally sustainable way. Two wastewaters were collected from a Portuguese poultry slaughterhouse (poultry raw - PR and poultry flocculated - PF) and the bioremediation was evaluated. The performance of microalga biomass growth and biochemical composition were assessed for two illumination sources (fluorescent vs LEDs). S. obliquus achieved positive results when grown in highly contaminated agro-industrial wastewater from the poultry industry, independently of the light source. The wastewater bioremediation revealed results higher than 97% for both ammonium and phosphate removal efficiency, for a cultivation time of 13 days. The saponifiable matter obtained from the biomass of the microalga cultures was, on average, 11 and 27% (m/m) with PR and PF wastewater, respectively. In opposition, higher sugar content was obtained from microalgae biomass grown in PR wastewater (average 34% m/m) in comparison to PF wastewater (average 23% m/m), independently of the illumination source. Therefore, biomass obtained with PR wastewater will be more appropriate as a raw material for bioethanol/biohydrogen production (higher sugar content) while biomass produced in PF wastewater will have a similar potential as feedstock for both biodiesel and bioethanol/biohydrogen production (similar lipid and sugar content).

ACS Style

Ana Cristina Oliveira; Ana Barata; Ana P. Batista; Luísa Gouveia. Scenedesmus obliquus in poultry wastewater bioremediation. Environmental Technology 2018, 40, 3735 -3744.

AMA Style

Ana Cristina Oliveira, Ana Barata, Ana P. Batista, Luísa Gouveia. Scenedesmus obliquus in poultry wastewater bioremediation. Environmental Technology. 2018; 40 (28):3735-3744.

Chicago/Turabian Style

Ana Cristina Oliveira; Ana Barata; Ana P. Batista; Luísa Gouveia. 2018. "Scenedesmus obliquus in poultry wastewater bioremediation." Environmental Technology 40, no. 28: 3735-3744.

Journal article
Published: 01 April 2018 in Renewable Energy
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ACS Style

Ana Paula Batista; Luísa Gouveia; Paula A.S.S. Marques. Fermentative hydrogen production from microalgal biomass by a single strain of bacterium Enterobacter aerogenes – Effect of operational conditions and fermentation kinetics. Renewable Energy 2018, 119, 203 -209.

AMA Style

Ana Paula Batista, Luísa Gouveia, Paula A.S.S. Marques. Fermentative hydrogen production from microalgal biomass by a single strain of bacterium Enterobacter aerogenes – Effect of operational conditions and fermentation kinetics. Renewable Energy. 2018; 119 ():203-209.

Chicago/Turabian Style

Ana Paula Batista; Luísa Gouveia; Paula A.S.S. Marques. 2018. "Fermentative hydrogen production from microalgal biomass by a single strain of bacterium Enterobacter aerogenes – Effect of operational conditions and fermentation kinetics." Renewable Energy 119, no. : 203-209.

Article
Published: 22 August 2017 in Engineering in Life Sciences
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The atmospheric CO2 increase is considered the main cause of global warming. Microalgae are photosynthetic microorganisms that can help in CO2 mitigation and at the same time produce value-added compounds. In this study, Scenedesmus obliquus, Chlorella vulgaris, and Chlorella protothecoides were cultivated under 0.035 (air), 5 and 10% (v/v) of CO2 concentrations in air to evaluate the performance of the microalgae in terms of kinetic growth parameters, theoretical CO2 biofixation rate, and biomass composition. Among the microalgae studied, S. obliquus presented the highest values of specific growth rate (μ = 1.28 d−1), maximum productivities (Pmax = 0.28 g L−1d−1), and theoretical CO2 biofixation rates (0.56 g L−1d−1) at 10% CO2. The highest oil content was found at 5% CO2, and the fatty acid profile was not influenced by the concentration of CO2 in the inflow gas mixture and was in compliance with EN 14214, being suitable for biodiesel purposes. The impact of the CO2 on S. obliquus cells’ viability/cell membrane integrity evaluated by the in-line flow cytometry is quite innovative and fast, and revealed that 86.4% of the cells were damaged/permeabilized in cultures without the addition of CO2.

ACS Style

Joana Assunção; Ana Paula Batista; João Manoel; Teresa Lopes Da Silva; Paula Marques; AlessanRSS Reis; Luísa Gouveia. CO2 utilization in the production of biomass and biocompounds by three different microalgae. Engineering in Life Sciences 2017, 17, 1126 -1135.

AMA Style

Joana Assunção, Ana Paula Batista, João Manoel, Teresa Lopes Da Silva, Paula Marques, AlessanRSS Reis, Luísa Gouveia. CO2 utilization in the production of biomass and biocompounds by three different microalgae. Engineering in Life Sciences. 2017; 17 (10):1126-1135.

Chicago/Turabian Style

Joana Assunção; Ana Paula Batista; João Manoel; Teresa Lopes Da Silva; Paula Marques; AlessanRSS Reis; Luísa Gouveia. 2017. "CO2 utilization in the production of biomass and biocompounds by three different microalgae." Engineering in Life Sciences 17, no. 10: 1126-1135.

Journal article
Published: 01 July 2017 in Molecules
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Diacronema vlkianum is a marine microalgae for which supposed health promoting effects have been claimed based on its phytochemical composition. The potential use of its biomass as health ingredient, including detox-shakes, and the lack of bioavailability studies were the main concerns. In order to evaluate the microalgae-biomass assimilation and its health-benefits, single-dose (CD1-mice) studies were followed by 66-days repeated-dose study in Wistar rats with the highest tested single-dose of microalgae equivalent to 101 mg/kg eicosapentaenoic acid + docosahexaenoic acid (EPA+DHA). Microalgae-supplementation modulated EPA and docosapentaenoic acid enrichment at arachidonic acid content expenditure in erythrocytes and liver, while increasing EPA content of heart and adipose tissues of rats. Those fatty acid (FA) changes confirmed the D. vlkianum-biomass FA assimilation. The principal component analyses discriminated brain from other tissues, which formed two other groups (erythrocytes, liver, and heart separated from kidney and adipose tissues), pointing to a distinct signature of FA deposition for the brain and for the other organs. The improved serum lipid profile, omega-3 index and erythrocyte plasticity support the cardiovascular benefits of D. vlkianum. These results bolster the potential of D. vlkianum-biomass to become a “heart-healthy” food supplement providing a safe and renewable source of bioavailable omega-3 FA.

ACS Style

Cristina De Mello-Sampayo; Angela Paterna; Ambra Polizzi; Diana Duarte; Irineu Batista; Rui Pinto; Patrícia Gonçalves; Anabela Raymundo; Ana P. Batista; Luísa Gouveia; Beatriz Silva-Lima; Narcisa M. Bandarra. Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats. Molecules 2017, 22, 1097 .

AMA Style

Cristina De Mello-Sampayo, Angela Paterna, Ambra Polizzi, Diana Duarte, Irineu Batista, Rui Pinto, Patrícia Gonçalves, Anabela Raymundo, Ana P. Batista, Luísa Gouveia, Beatriz Silva-Lima, Narcisa M. Bandarra. Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats. Molecules. 2017; 22 (7):1097.

Chicago/Turabian Style

Cristina De Mello-Sampayo; Angela Paterna; Ambra Polizzi; Diana Duarte; Irineu Batista; Rui Pinto; Patrícia Gonçalves; Anabela Raymundo; Ana P. Batista; Luísa Gouveia; Beatriz Silva-Lima; Narcisa M. Bandarra. 2017. "Evaluation of Marine Microalga Diacronema vlkianum Biomass Fatty Acid Assimilation in Wistar Rats." Molecules 22, no. 7: 1097.

Chapter
Published: 26 March 2017 in Lecture Notes in Energy
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The biorefinery concept harbors a wide range of conversion technologies in order to take advantage of all the components of a feedstock. Among those technologies, a wide range of biological/biochemical processes can be envisaged to generate products such as biofuels, value-addedproducts, and other chemical building blocks. These processes are generally defined as fermentative although each of them requires special operational conditions (e.g., anaerobic environment, illumination) and/or different microorganisms (bacteria, yeasts, cyanobacteria, algae). Furthermore, when processing a complex raw material such as a lignocellulosic material or microbial biomass, there is often a need for a previous step to break down the matrix providing suitable substrates for their subsequent conversion. This biomass hydrolysis may be achieved through the action of specialized enzymes which are chosen according to the task to be accomplished, usually cellulases or hemicellulases. This chapter gives short descriptions of all the biological/biochemical processes used today in biorefineries taking into consideration the final product of interest.

ACS Style

Luisa Gouveia; Paula C. Passarinho. Biomass Conversion Technologies: Biological/Biochemical Conversion of Biomass. Lecture Notes in Energy 2017, 99 -111.

AMA Style

Luisa Gouveia, Paula C. Passarinho. Biomass Conversion Technologies: Biological/Biochemical Conversion of Biomass. Lecture Notes in Energy. 2017; ():99-111.

Chicago/Turabian Style

Luisa Gouveia; Paula C. Passarinho. 2017. "Biomass Conversion Technologies: Biological/Biochemical Conversion of Biomass." Lecture Notes in Energy , no. : 99-111.

Review
Published: 07 November 2016 in Current Biotechnology
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Alberto Reis; Luisa Gouveia. Low Cost Microalgal Production for Biofuels: A Review. Current Biotechnology 2016, 5, 266 -276.

AMA Style

Alberto Reis, Luisa Gouveia. Low Cost Microalgal Production for Biofuels: A Review. Current Biotechnology. 2016; 5 (4):266-276.

Chicago/Turabian Style

Alberto Reis; Luisa Gouveia. 2016. "Low Cost Microalgal Production for Biofuels: A Review." Current Biotechnology 5, no. 4: 266-276.

Journal article
Published: 01 June 2016 in Algal Research
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This work is part of a LIFE project to treat urban wastewater from Águas da Figueira (AdF, Figueira da Foz, PT) using a vertical tubular photobioreactor (PBR) prototype (150 L), to be scaled up and integrated in a waste water treatment plant (WWTP). The PBR was inoculated with three different microalgae: Chlorella vulgaris (Cv), Scenedesmus obliquus (Sc) and Consortium C (ConsC), isolated from the effluent. The study intends to find the best microalga in terms of wastewater remediation, biomass productivity and quality, for further uses, such as biofuel, biofertilizer and bioplastic production. The experiments achieved volumetric productivities of 0.1 g/L·d (Cv), 0.4 g/L·d (Sc) and 0.9 g/L·d (ConsC). The maximum removals attained by Cv, Sc and ConsC were: 84, 95 and 98% for total nitrogen; 95, 92 and 100% for phosphorus; and 36, 63 and 64% for COD, respectively. The treated water had values that are in accordance with environmental legislation (Directive 98/15/CE). Electrocoagulation was tested and resulted in an energy saving of 89%, compared with centrifugation alone. For drying the biomass, a solar dryer was used. Costs of overall processes versus conventional technologies are discussed and compared with other facilities and target values.

ACS Style

Luísa Gouveia; Sofia Graça; Catarina Sousa; Lucas Ambrosano; Belina Ribeiro; Elberis P. Botrel; Pedro Castro Neto; Ana F. Ferreira; Carla Silva. Microalgae biomass production using wastewater: Treatment and costs. Algal Research 2016, 16, 167 -176.

AMA Style

Luísa Gouveia, Sofia Graça, Catarina Sousa, Lucas Ambrosano, Belina Ribeiro, Elberis P. Botrel, Pedro Castro Neto, Ana F. Ferreira, Carla Silva. Microalgae biomass production using wastewater: Treatment and costs. Algal Research. 2016; 16 ():167-176.

Chicago/Turabian Style

Luísa Gouveia; Sofia Graça; Catarina Sousa; Lucas Ambrosano; Belina Ribeiro; Elberis P. Botrel; Pedro Castro Neto; Ana F. Ferreira; Carla Silva. 2016. "Microalgae biomass production using wastewater: Treatment and costs." Algal Research 16, no. : 167-176.

Journal article
Published: 01 January 2016 in Journal of Marine Biology & Oceanography
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Janelas J Gouveia L. Microalga Nannochloropsis sp. Biomass for Biodiesel Production: Conventional (Cell Disruption) and in situ Transesterification. Journal of Marine Biology & Oceanography 2016, 5, 1 .

AMA Style

Janelas J Gouveia L. Microalga Nannochloropsis sp. Biomass for Biodiesel Production: Conventional (Cell Disruption) and in situ Transesterification. Journal of Marine Biology & Oceanography. 2016; 5 (1):1.

Chicago/Turabian Style

Janelas J Gouveia L. 2016. "Microalga Nannochloropsis sp. Biomass for Biodiesel Production: Conventional (Cell Disruption) and in situ Transesterification." Journal of Marine Biology & Oceanography 5, no. 1: 1.

Journal article
Published: 01 August 2015 in Energy
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The biological hydrogen production from Spirogyra sp. biomass was studied in a SBR (sequential batch reactor) equipped with a biogas collecting and storage system. Two acid hydrolysis pre-treatments (1N and 2N H2SO4) were applied to the Spirogyra biomass and the subsequent fermentation by Clostridium butyricum DSM 10702 was compared. The 1N and 2N hydrolyzates contained 37.2 and 40.8 g/L of total sugars, respectively, and small amounts of furfural and HMF (hydroxymethylfurfural). These compounds did not inhibit the hydrogen production from crude Spirogyra hydrolyzates. The fermentation was scaled up to a batch operated bioreactor coupled with a collecting system that enabled the subsequent characterization and storage of the biogas produced. The cumulative hydrogen production was similar for both 1N and 2N hydrolyzate, but the hydrogen production rates were 438 and 288 mL/L.h, respectively, suggesting that the 1N hydrolyzate was more suitable for sequential batch fermentation. The SBR with 1N hydrolyzate was operated continuously for 13.5 h in three consecutive batches and the overall hydrogen production rate and yield reached 324 mL/L.h and 2.59 mol/mol, respectively. This corresponds to a potential daily production of 10.4 L H2/L Spirogyra hydrolyzate, demonstrating the excellent capability of C. butyricum to produce hydrogen from microalgal biomass

ACS Style

Joana Ortigueira; Tiago Pinto; Luísa Gouveia; Patrícia Moura. Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum. Energy 2015, 88, 528 -536.

AMA Style

Joana Ortigueira, Tiago Pinto, Luísa Gouveia, Patrícia Moura. Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum. Energy. 2015; 88 ():528-536.

Chicago/Turabian Style

Joana Ortigueira; Tiago Pinto; Luísa Gouveia; Patrícia Moura. 2015. "Production and storage of biohydrogen during sequential batch fermentation of Spirogyra hydrolyzate by Clostridium butyricum." Energy 88, no. : 528-536.

Journal article
Published: 01 August 2015 in Fuel
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Scenedesmus obliquus biomass was used as a feedstock for comparing the biological production of hydrogen by two different types of anaerobic cultures: a heat-treated mixed culture from a wastewater treatment plant and Clostridium butyricum DSM 10702. The influence of the incubation temperature and the carbon source composition were evaluated in order to select the best production profile according to the characteristics of the microalgal biomass. C. butyricum showed a clear preference for monomeric sugars and starch, the latter being the major storage compound in microalgae. The highest H2 production reached by this strain from starch was 468 mL/g, whereas the mixed culture incubated at 37 C (LE37) produced 241 mL/g. When the mixed culture was incubated at 58 C (LE58), a significant increase in the H2 production occurred when xylose and xylan were used as carbon and energy source. The highest H2 yield reached by the LE37 culture or in co-culture with C. butyricum was 1.52 and 2.01 mol/mol of glucose equivalents, respectively. However, the ratio H2/CO2 (v/v) of the biogas produced in both cases was always lower than the one produced by the pure strain. In kinetic assays, C. butyricum attained 153.9 mL H2/L h from S. obliquus biomass within the first 24 h of incubation, with a H2 yield of 2.74 mol/mol of glucose equivalents. H2 production was accompanied mainly by acetate and butyrate as coproducts. In summary, C. butyricum demonstrated a clear supremacy for third generation bioH2 production from S. obliquus biomass

ACS Style

Joana Ortigueira; Luís Alves; Luisa Gouveia; Patrícia Moura. Third generation biohydrogen production by Clostridium butyricum and adapted mixed cultures from Scenedesmus obliquus microalga biomass. Fuel 2015, 153, 128 -134.

AMA Style

Joana Ortigueira, Luís Alves, Luisa Gouveia, Patrícia Moura. Third generation biohydrogen production by Clostridium butyricum and adapted mixed cultures from Scenedesmus obliquus microalga biomass. Fuel. 2015; 153 ():128-134.

Chicago/Turabian Style

Joana Ortigueira; Luís Alves; Luisa Gouveia; Patrícia Moura. 2015. "Third generation biohydrogen production by Clostridium butyricum and adapted mixed cultures from Scenedesmus obliquus microalga biomass." Fuel 153, no. : 128-134.

Journal article
Published: 01 May 2015 in Bioresource Technology
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The aim of the present work was the simultaneous treatment of urban wastewater using microalgae and the energetic valorization of the obtained biomass. Chlorella vulgaris (Cv), Scenedesmus obliquus (Sc) and a naturally occurring algal Consortium C (ConsC) were grown in an urban wastewater. The nutrient removals were quite high and the treated water fits the legislation (PT Dec-Lei 236/98) in what concerns the parameters analysed (N, P, COD). After nutrient depletion the microalgae remained two more weeks in the photobioreactor (PBR) under nutritional stress conditions, to induce sugar accumulation (22-43%). The stressed biomass was converted into biohydrogen (bioH2), a clean energy carrier, through dark fermentation by a strain of the bacteria Enterobacter aerogenes. The fermentation kinetics were monitored and fitted to a modified Gompertz model. The highest bioH2 production yield was obtained for S. obliquus (56.8 mL H2/gVS) which was very similar when using the same algae grown in synthetic media.

ACS Style

Ana Paula Batista; Lucas Ambrosano; Sofia Graça; Catarina Sousa; Paula A.S.S. Marques; Belina Ribeiro; Elberis P. Botrel; Pedro Castro Neto; Luisa Gouveia. Combining urban wastewater treatment with biohydrogen production – An integrated microalgae-based approach. Bioresource Technology 2015, 184, 230 -235.

AMA Style

Ana Paula Batista, Lucas Ambrosano, Sofia Graça, Catarina Sousa, Paula A.S.S. Marques, Belina Ribeiro, Elberis P. Botrel, Pedro Castro Neto, Luisa Gouveia. Combining urban wastewater treatment with biohydrogen production – An integrated microalgae-based approach. Bioresource Technology. 2015; 184 ():230-235.

Chicago/Turabian Style

Ana Paula Batista; Lucas Ambrosano; Sofia Graça; Catarina Sousa; Paula A.S.S. Marques; Belina Ribeiro; Elberis P. Botrel; Pedro Castro Neto; Luisa Gouveia. 2015. "Combining urban wastewater treatment with biohydrogen production – An integrated microalgae-based approach." Bioresource Technology 184, no. : 230-235.