Experimental studies were conducted to evaluate 16 advanced breeding lines of cowpea (Vigna unguiculata (L) Walp) for genetic variability, heritability and correlation between maturity and yield related traits. The genotypes exhibited significant (P < 0.05) differences for the eight traits evaluated viz., number of days to 50 % flowering, number of days to 90 % pod maturity, plant height at maturity, number of pods per plant, number of seeds per pod, pod yield, grain yield and hundred seed weight. SARI-3-11-100, SARI-6-2-6, SARVX-09-004 and IT07K-299-6 had grain yields of 1.99 ± 0.30, 1.88 ± 0.20, 1.95 ± 0.30 and 1.91 ± 0.20 t/ha, respectively, which were significantly higher than the check (Songotra) (1.68 ± 0.01 t/ha). In addition, SARI-5-5-5 matured significantly earlier than the check but no significant difference was observed for grain yield. The higher value of phenotypic component compared to the corresponding genotypic component for all the traits suggest that there was an environmental influence on the performance of the genotypes. Hence, the need for multi-location evaluation of the promising lines for onward release if found stable. The information provided in this study, can be exploited in cowpea breeding program.
Cassava (Manihot esculentaCrantz) is an important industrial and staple crop due to its high starch content, low input requirement, and resilience which makes it an ideal crop for sustainable agricultural systems and marginal lands in the tropics. However, the lack of genomic information on local genetic resources has impeded efficient conservation and improvement of the crop and the exploration of its full agronomic and breeding potential. This work was carried out to obtain information on population structure and extent of genetic variability among some local landraces conserved at the Plant Genetic Resources Research Institute, Ghana and exotic cassava accessions with Diversity Array Technology based SilicoDArT and SNP markers to infer how the relatedness in the genetic materials can be used to enhance germplasm curation and future breeding efforts. A total of 10521 SilicoDArT and 10808 SNP markers were used with varying polymorphic information content (PIC) values. The average PIC was 0.36 and 0.28 for the SilicoDArT and SNPs respectively. Population structure and average linkage hierarchical clustering based on SNPs revealed two distinct subpopulations and a large number of admixtures. Both DArT platforms identified 22 landraces as potential duplicates based on Gower’s genetic dissimilarity. The expected heterozygosity which defines the genetic variation within each subpopulation was 0.008 for subpop1 which were mainly landraces and 0.391 for subpop2 indicating the homogeneous and admixture nature of the two subpopulations. Further analysis upon removal of the duplicates increased the expected heterozygosity of subpop1 from 0.008 to 0.357. A mantel test indicated strong interdependence (r = 0.970; P < 0.001) between SilicoDArT and DArTSeq SNP genotypic data suggesting both marker platforms as a robust system for genomic studies in cassava. These findings provide important information for efficientex-situconservation of cassava, future heterosis breeding, and marker-assisted selection (MAS) to enhance cassava improvement.
10 September 2016 in Genetic Resources and Crop Evolution