The Euthyneura are considered to be the most successful and diverse group of Gastropoda. Phylogenetically, they are riven with controversy. Previous morphology-based phylogenetic studies have been greatly hampered by rampant parallelism in morphological characters or by incomplete taxon sampling. Based on sequences of nuclear 18S rRNA and 28S rRNA as well as mitochondrial 16S rRNA and COI DNA from 56 taxa, we reconstructed the phylogeny of Euthyneura utilising Maximum Likelihood and Bayesian inference methods. The evolution of colonization of freshwater and terrestrial habitats by pulmonate Euthyneura, considered crucial in the evolution of this group of Gastropoda, is reconstructed with Bayesian approaches.

Background: Snails such asGalba truncatulaare hosts for trematode flukes causing fascioliasis, a zoonosis that is a major public health problem.Galba truncatulahas recently been shown to be a cryptic species complex. African populations ofGalbaspp. are not yet studied using molecular assessments and is imperative to do so and reconstruct the centre of origin ofGalbaand to understand when and by what means it may have colonized the highlands of Africa and to what extent humans might have been involved in that process.Methods: Samples from all known sub-ranges throughout Africa and new samples from Europe and Asia were obtained. We used a combination of two mitochondrial (cox1 and16S) and one nuclear (ITS2) markers and phylogenetic, divergence time estimates and phylogeographical methods to determine the identity and biogeographical affinities. We also reconstructed the colonization history including the likely mode of dispersal and tested for the presence of crypticGalbaspecies in Africa.Results: Galba truncatulais restricted to the Palaearctic region of the continent, namely Morocco. All sub-Saharan populations proved to be a distinct species according to the phylogenetic analyses and genetic distance. We propose to use the existing nameGalba mweruensis(Connolly, 1929) for this species which is morphologically indistinguishable from the other two species hitherto known to occur in northern Africa, i.e.G. truncatulaandG. schirazensis. Sub-tropical Africa has been colonized only once in either the Pliocene and possibly Miocene. Diversification withinG. mweruensisis dated to the Plio-Pleistocene and thus human-mediated dispersal can be ruled out for the initial colonization of the isolated mountain ranges. There are potentially even more cryptic species in high altitude areas of Africa as outlined by the distinctness of the population found at the top of Mt. Elgon, Uganda.Conclusions: From a novel genetic inspection of available African material, a hitherto neglected distinct species,G. mweruensis, now appears a major host ofF. hepaticathroughout sub-Saharan Africa. A closer examination of trematode parasites hosted by this species is needed in order to understand transmission patterns in highlands throughout eastern and southern Africa. We encourage future studies to inspect other high altitudes areas in Africa in light of parasites of either veterinary or medical importance.

Immediate conservation measures for world-wide freshwater resources are of eminent importance. This is particularly true for so-called ancient lakes. While these lakes are famous for being evolutionary theatres, often displaying an extraordinarily high degree of biodiversity and endemism, in many cases these biota are also experiencing extreme anthropogenic impact. Lake Ohrid, a major European biodiversity hotspot situated in a trans-frontier setting on the Balkans, is a prime example for a lake with a magnitude of narrow range endemic taxa that are under increasing anthropogenic pressure. Unfortunately, evidence for a "creeping biodiversity crisis" has accumulated over the last decades, and major socio-political changes have gone along with human-mediated environmental changes. Based on field surveys, monitoring data, published records, and expert interviews, we aimed to (1) assess threats to Lake Ohrids' (endemic) biodiversity, (2) summarize existing conservation activities and strategies, and (3) outline future conservation needs for Lake Ohrid. We compiled threats to both specific taxa (and in cases to particular species) as well as to the lake ecosystems itself. Major conservation concerns identified for Lake Ohrid are: (1) watershed impacts, (2) agriculture and forestry, (3) tourism and population growth, (4) non-indigenous species, (5) habitat alteration or loss, (6) unsustainable exploitation of fisheries, and (7) global climate change. Among the major (well-known) threats with high impact are nutrient input (particularly of phosphorus), habitat conversion and silt load. Other threats are potentially of high impact but less well known. Such threats include pollution with hazardous substances (from sources such as mines, former industries, agriculture) or climate change. We review and discuss institutional responsibilities, environmental monitoring and ecosystem management, existing parks and reserves, biodiversity and species measures, international conservation activities, and ongoing research on conservation and raising of public awareness. Following this summary, we evaluate the status quo and future of Lake Ohrid and its biota. A comprehensive conservation strategy should include measures that result in an immediate reduction of nutrient input, particularly with phosphorus, in order to slow down the ongoing eutrophication process. The existing watershed management should become more effective. Implementation and particularly with a view to the enforcement of national laws should be enhanced. Increased research on the lakes' limnology, biodiversity, and conservation management practices are necessary. The latter research should identify conservation priorities. Public awareness should be enhanced. Facing these parallel needs to protect the unique biodiversity of Lake Ohrid, we suggest urging (a) implementation and enforcement of the General Management Plan that would ensure long-term integrated and sustainable use of the lake and its watershed, (b) scientific studies on ecology, biodiversity and effects of human impact, (c) the establishment of Core Conservation areas (CCA), including underwater reserves, and (d) Coastal Zone Management (CZM) areas that would constitute buffer zones for the CCAs around the lake. Given the number of identified threats, it is clear that only concerted international action can stop or at least slow down further degradation of Lake Ohrid and the creeping biodiversity crisis already evident. All conservation activities should, among others, ultimately lead to a trans-boundary major conservation area of the Ohrid-Prespa region that would allow long-term integration of both humans and nature.

The formation of the East African Rift System has decisively influenced the distribution and evolution of tropical Africa’s biota by altering climate conditions, by creating basins for large long-lived lakes, and by affecting the catchment and drainage directions of river systems. However, it remains unclear how rifting affected the biogeographical patterns of freshwater biota through time on a continental scale, which is further complicated by the scarcity of molecular data from the largest African river system, the Congo.