Today, the use of microbial origin biological products in poultry farming is widespread. However, it was found that the use of living bacteria in such preparations is not always effective due to their inactivation under the action of enzymes located in the gastrointestinal tract. In this connection, it is promising to use their destroyed forms (lysates), which also have high biological properties. The aim of the scientific work was to assess the quality of the developed hydrolyzate of lactic acid bacteria and its compliance with regulatory documents. It was established that the studied lactobacillus hydrolyzate meets the requirements of the regulatory documentation for this biological product, does not have toxic and irritating effects on the body of laboratory animals, and therefore can be offered in industrial poultry farming to increase the safety and productivity of farm poultry.

When developing veterinary or feed preparations, a mandatory procedure is the study of their toxicological properties in living bio-objects. The purpose of this research work was to study the toxicological effect of probiotic on the basis of autochthonous microflora of birds by identifying the parameters of acute, subacute and chronic toxicity, as well as irritating characteristics in experimental laboratory animals. In studies of subacute and chronic toxicity, the pharmacodynamics of a probiotic was studied by its effect on individual metabolic parameters, morphobiochemical composition of blood, and also the growth rate of experimental laboratory animals. A complex of toxicological studies of the developed biological product showed that the probiotic does not exhibit toxic and irritating properties, stimulates hematopoiesis, erythropoiesis, there is a slight increase in the mass of laboratory animals in connection with which it can be used for its intended purpose, regardless of the dose.

Context: Malaria remains a severe global health concern, exacerbated by increasing resistance to antimalarial agents in Plasmodium falciparum. Indonesian medicinal plants have demonstrated pharmacological potential, although their application against the calcium-dependent protein kinase 2 (CDPK2) of P. falciparum has not been previously explored. Aims: To assess the potential of Indonesian herbal compounds as inhibitors of P. falciparum CDPK2 using a range of in silico computational techniques. Methods: The structure of CDPK2 was retrieved from the Protein Data Bank (PDB ID: 4MVF) and prepared using the YASARA software. Structural validation was performed using Ramachandran plot analysis, ERRAT, and Verify 3D. The ligands were derived from documented Indonesian herbal compounds. Molecular docking was performed using AutoDock Vina integrated within YASARA, and the best-scoring ligands were subjected to DFT calculations using ORCA 5.0. ADME and biological activity predictions were performed using SwissADME and PASS, respectively. The stability of the most promising complex was assessed via normal mode analysis (NMA) using iMODS. Results: Among the screened compounds, epicatechin 3-O-(3-O-methylgallate) and remerin exhibited the highest binding affinities. While DFT analysis confirmed favorable electronic properties for both, remerin alone met the drug-likeness criteria based on ADME evaluation. Normal mode analysis demonstrated that the CDPK2-remerin complex maintained its structural stability throughout the simulation. Conclusions: Remerin was identified as a promising inhibitor of CDPK2 based on its binding affinity, pharmacokinetic properties, and molecular dynamics stability. Further in vitro and in vivo investigations are required to validate these findings.

Авторами работы проведены исследования по изучению влияния клеточного и напольного способов содержания перепелов Техасской породы, а также условий кормления с применением в их рационе кормовой добавки на основе биомассы грибной культуры Cephaliophora tropica, насыщенной полезными микроэлементами и аминокислотами, на показатели сохранности, прироста, поедаемости комбикорма, мясной продуктивности, морфобиохимические данные крови, а также качество продукции перепеловодства. Результаты научно-хозяйственных экспериментов продемонстрировали, что, независимо от способа выращивания птицы, наилучшие данные были получены в группах, в рационе которых была исследуемая кормовая добавка. При этом оптимальным условием содержания перепелов для получения максимально положительных результатов является напольный способ. Так установлено, что в данной группе птиц сохранность перепелов составила 94,0 %, живая масса на 56-й день исследований составила 405,81 г, что на 12,9 % выше, чем в контрольной группе и на 4,4 % по отношению к одноименной опытной группе, конверсия ниже на 3,7-9,3 %. Выявлено, что грибной автолизат в опытных группах оказал положительное влияние на морфологические и биохимические показатели крови анализируемых перепелов, так как установлена стимуляция гемопоэза, а также достоверно усиливается обмен белков в организме птиц. Самые высокие показатели убойного выхода потрошенной тушки зафиксированы во 2-й опытной группе - 75,6 %, где перепелов содержали напольным способом с применением кормовой добавки Альбит-Био. При анализе качества мяса птицы установлено, что у перепелов опытных групп оно было более полноценным, диетическим и по результатам дегустационной оценки получило наивысший...

The article presents data on the use of modern methods of analysis, isolation and identification of representatives of the microbiocenosis of the ceca of the intestines of industrial pigs raised using intensive technology. Bacterial metagenomic analysis was used to study the composition of microflora of various taxonomic groups in the chyme of industrial pigs. Classical microbiological research methods were used to isolate the dominant representatives of the genus Lactobacillus. Identification of the probiotically relevant microbial cultures was performed by mass spectrometric analysis using MALDI-TOF MS in a BactoSCREEN spectrometer, as well as by nucleotide sequencing of the 16S rRNA gene. Full-genome sequencing of isolated dominant pure cultures of the genus Lactobacillus was carried out. As a result of the research, it was found that in the cecum of the intestines of suckling piglets, growing and fattening pigs there is a diversity of microbial communities, which changes in quantitative proportions with age. The results of mass spectrometric analysis revealed the presence of protein spectra of important representatives of bacteria of the genus Lactobacillus, among which two species, Lactobacillus amylovorus and Limosilactobacillus mucosae, were dominant, which were additionally confirmed by analyzing their nucleotide sequence of the 16S rRNA gene and by performing full-genome sequencing.