Ultrasmall glucose carbon dots rapidly cross the blood-spinal cord barrier and target neurons at the spinal and supraspinal network. The graphical abstract was created in BioRender.

Background/Objectives: The level of the sciatic nerve bifurcation is clinically relevant for diagnostic ultrasonography, ultrasound-guided popliteal sciatic nerve block, posterior-thigh interventions, and surgical approaches to the hip, thigh, and popliteal region. Although the sciatic nerve is classically described as dividing near the popliteal fossa, its terminal branching level may vary considerably. This study aimed to evaluate the level of sciatic nerve bifurcation and its morphometric relationship to clinically relevant anatomical landmarks, with particular emphasis on the posterior intercondylar reference line. Methods: This study was conducted on 56 formalin-fixed adult human lower-extremity specimens. The bifurcation level was categorized as femoral-level, gluteal-level, or separate emergence of the tibial and common fibular components. The sciatic nerve–piriformis relationship was assessed according to the Beaton and Anson classification. Morphometric measurements were obtained from the bifurcation point to the greater trochanter, ischial tuberosity, and posterior intercondylar reference line. Results: Femoral-level bifurcation was observed in 51 of 56 specimens (91.1%), gluteal-level bifurcation in 1 specimen (1.8%), and separate emergence in 4 specimens (7.1%). Type A was the predominant Beaton and Anson pattern, observed in 50 specimens (89.3%). Type B and Type D were each observed in 2 specimens (3.6%), and 2 specimens (3.6%) demonstrated an atypical superior gemellus-related course. All measurable bifurcation points were located proximal to the posterior intercondylar reference line. Among femoral-level bifurcations, the mean distance from this line to the bifurcation point was 8.67 ± 4.89 cm. No statistically significant sex- or side-based differences were observed in the available morphometric parameters. Conclusions: Sciatic nerve bifurcation most commonly occurred at the femoral level, but clinically relevant variations were observed. The posterior intercondylar reference line may provide a useful distal femoral orientation landmark for posterior knee and distal thigh assessment. However, it should complement, rather than replace, direct ultrasonographic visualization of the sciatic nerve and its terminal branches.