Introduction: The aim of this study was to evaluate potential protective effects of propolis on furan-induced hepatic damage by assessing the levels of malondialdehyde (MDA) and reduced glutathione (GSH), antioxidant enzyme activities, and histopathological changes in the liver. Material and Methods: Albino Wistar rats were divided into six groups: a control, propolis-treated (100 mg/kg b.w./day), low-dose furan-treated (furan-L group; 2 mg/kg b.w./day), high-dose furan-treated (furan-H group; 16 mg/kg b.w./day), furan-L+propolis treated, and furan-H+propolis treated group. Propolis and furan were applied by gavage; propolis for 8 days, and furan for 20 days in furan-L groups and 10 days in furan-H groups. Results: While MDA levels were elevated in furan-treated groups, levels of GSH and activities of antioxidant enzymes decreased (p < 0.001). The levels of MDA and GSH and activities of antioxidant enzymes were normal in the furan+propolis groups, especially in the furan-L+propolis group (p < 0.001). While the aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate pdehydrogenase activities were elevated in the furan-H treated group (p < 0.05 and p < 0.001), they were unchanged in the furan-L treated group. Histopathologically, several lesions were observed in the liver tissues of the furan-treated groups, especially in the higher-dose group. It was determined that these changes were milder in both of the furan+propolis groups. Conclusion: The results indicate that propolis exhibits good hepatoprotective and antioxidant potential against furan-induced hepatocellular damage in rats.

The positive effects of grape seed proanthocyanidin extract (GSPE) on bone health, which is a potent antioxidant, are known but its effects on fracture healing are not sufficiently covered in the literature. This study aims to investigate the effects of GSPE on fracture healing and biomechanics of healing bone. Sixty-four adult Wistar-Albino male rats were divided into 8 groups of 8 animals in each group. Osteotomy was performed to the right femurs of all groups except the negative control (G1) and positive control (G2) groups, and intramedullary Kirchner wire was used for fixation. GSPE was given to half of the rats (G2-G4-G6-G8) 100 mg/kg/day by oral gavage. The rats were sacrificed on the tenth (G3–G4), twentieth (G5–G6), and thirtieth (G1–G2–G7–G8) days, respectively, and histopathological, radiological, and biomechanical examinations were performed. Histopathological examination of the specimens from the callus tissues revealed that bone healing was more prominent in the groups supplemented with GSPE (G4, G6, G8). There was a statistically significant improvement in radiological recovery scores and callus volumes in groups with GSPE. When biomechanical strengths were evaluated, it was found that GSPE increased bone strength not only in fracture groups but also in the positive control group (G2). As a result, this study showed that GSPE, a potent anti-oxidant, had a positive effect on bone healing and improved mechanical strength of the healing bone.

Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1. Method: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group. Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group. Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

Bu çalışmanın amacı, doksorubisinin (DOX) neden olduğu kardiyotoksisite üzerine kımızın etkisini araştırmaktı. Yirmi sekiz Wistar-Albino erkek sıçan 4 gruba ayrıldı: Birinci gruba (kontrol) herhangi bir müdahale yapılmadı. İkinci gruba 7 gün gavaj yoluyla 2 ml/kg/gün, üçüncü gruba intraperitoneal DOX, dördüncü gruba kımız+DOX 20 mg/kg tek doz verildi. DOX uygulamasından 7 gün önce Kumiss uygulamasına başlandı ve 7 gün devam edildi. 7 On thkımız uygulamasının ilk gününde intraperitoneal olarak DOX uygulandı. Malondialdehit (MDA), indirgenmiş glutatyon (GSH) seviyeleri ve katalaz (CAT), glutatyon peroksidaz (GSH-Px), süperoksit dismutaz (SOD), glukoz-6-fosfat dehidrojenaz (G6PD) ve glutatyon-S- gibi antioksidan enzimler Kalp ve kan dokularında kardiyotoksisite patogenezindeki etkinliklerini belirlemek için transferaz (GST) aktiviteleri belirlendi. DOX grubu kontrol grubu ile karşılaştırıldığında MDA (p<0,001, p<0,001) ve GSH (p<0,001, p=0,002) düzeylerinde artış ve CAT'de azalma (p=0,001, p<0,001) , GSH-Px (p<0.001, p<0.001), G6PD (p<0.001, p=0.001) ve GST (p=0.003) aktiviteleri bulundu ve SOD aktivitesinde istatistiksel olarak anlamlı fark bulunmadı. Histopatolojik olarak dejenerasyon, nekroz, DOX uygulanan grupta kanama ve ödem gözlendi. DOX ile tedavi edilen grup ile karşılaştırıldığında, DOX ile kımız verilen grupta MDA, GSH düzeyleri ve antioksidan enzim aktivitelerinin kontrol grubu değerlerine ulaştığı görüldü. Sonuç olarak, lipid peroksidasyon ürünlerindeki artış ve antioksidan enzimlerdeki azalmanın, güçlü bir kemoterapötik ilaç olan DOX ile indüklenen kardiyotoksisitenin patogenezinde rol oynayabileceği belirlendi ve kırmızının DOX kaynaklı oksidatif hasara karşı koruduğu gösterildi.

Objective. Many applications and pharmaceutical active substances have been used since the beginning of human history to ensure healthy and rapid healing of wounds. This study aimed to determine the effectiveness of microcurrent electrical therapy on wound healing. Material and methods. A full-thickness sectional skin wound of 4 cm2 in size was created in the interscapular region of the 14 rabbits used in the study. Microcurrent electrotherapy was applied to rabbits with excisional skin wounds for 10 days. Results. At the end of the 10th day, when the wound closure rates were compared, it was determined that the rabbits in the microcurrent electrotherapy (ME) group were in better condition than the rabbits in the control group (CG). In the histopathological scoring, it was determined that there was no statistical difference between the two groups (p ˃0.05). When evaluated biochemically, MDA levels of rabbits in the ME group were determined to be statistically significantly higher. GSH, CAT, GSH-Px levels were found to be statistically significantly lower in rabbits in the ME group (P˂0.001). Conclusions. As a result, according to the findings from this study, it was determined that microcurrent electrotherapy application clinically accelerated wound healing. However, due to the short study period, no histopathological difference could be detected between the groups in wound healing.