Natural products remain an important source of structurally diverse compounds for the discovery of new antimicrobial agents. Gliricidia sepium (Jacq.) Kunth ex Walp. is a medicinal species widely distributed in tropical regions and is recognized as a source of flavonoids, phenolic acids, coumarins, glycosides, and other specialized metabolites with diverse biological activities. Previous phytochemical investigations of this species have reported compounds such as oxytroside, o-coumaric acid, and melilotic acid, highlighting its potential as a source of bioactive natural products. In the present study, the influence of sequential solvent extraction on the recovery of antibacterial metabolites from G. sepium leaves was evaluated. Dried leaves were sequentially extracted by maceration with n-hexane, ethyl acetate, and methanol, and the resulting crude extracts were tested against Staphylococcus aureus using the agar disk diffusion assay at concentrations of 12.5, 25, 50, 100, and 200 μg/mL. Antibacterial activity varied according to solvent polarity and extract concentration. The methanolic extract exhibited the highest activity, producing an inhibition zone of 1.1 cm at 200 μg/mL, followed by the ethyl acetate extract (0.8 cm), whereas the hexane extract showed only slight activity (0.7 cm). The positive control produced inhibition zones ranging from 2.8 to 3.7 cm. These findings indicate that sequential solvent extraction influences the recovery of antibacterial constituents from G. sepium and support further bioactivity-guided fractionation and phytochemical characterization of the most active extracts.