Environmental stresses such as drought, cold and heat in Pannonia Basin significantly endanger the cell activity, plant growth and yields in wheat, which is one of the most strategic cereal grain crops in the world. As science and technology advance, new tools are developed while old ones are refined for use by breeders. Higher agronomical efficiency is possible by combining new and old tools to bridge the abiotic stress issues. Five cultivars of winter wheat (Simonida, Petrija, Ljubica, Zvezdana and NS Mila), were used in the study carried out at our experimental field (Novi Sad as a center of Pannonia Basin) across three consecutive growing seasons to assess genetic interaction and the level of tolerance and adaptability of different cultivars to abiotic stresses like drought conditions, cold and heat. Four quantitative yield components and grain yield were analiyed to assess expression of adapted genotypes in the region. Among the cultivars, Simonida, which has been in use for the longest period, exhibited the most consistent yield response. Additionally, it demonstrated some degree of partial tolerance to abiotic stress conditions, possibly due to the integration of stress memory into its genetic code, supported by statistical analysis findings.

Creation of new higher yield cultivars, adaptation of existing germplasm to a global climate change, increasing resistance to diseases in new genotypes are some of the tasks that breeding have in front of it. The objectives of this research were to assess GE interaction in two different environments across two vegetation seasons and to do association analysis based on the results of the phenotypic and molecular evaluation. Grain samples were obtained from 96 winter wheat cultivars grown in 2011/12 and 2012/13 at two locations in the South Pannonia Basin region and population was profiled with 28 microsatellites. The share of genotype is high and amounts 24.84%, while the share of environments was 21.06%, when yield was evaluated. The GE interaction was also statistically significant and amounts 51.58% of the total variance. Microsatellites that exhibited a relation with yield by GLM and MLM model were: gwm357, gwm339, cfa2114, gwm631, gwm495, gwm190, barc1121 and gwm437. Markers that have demonstrated the stability of the relationship with yield in different environments can be recommended as potentially useful in wheat breeding.

Generation time is the principal bottleneck constraining genetic gain in plant breeding. Speed breeding (SB) addresses this by simultaneously managing the photoperiod, light spectrum and intensity, temperature, CO₂ concentration, mineral nutrition, growing substrate volume, and post-harvest seed dormancy, enabling four to seven generations per year in long-day cereals and legumes, and four to five generations in optimised short-day systems. This review evaluates SB against the conventional pedigree framework; synthesises validated environmental parameters and crop-specific protocols; and examines principal SB applications in hybridisation, genomic selection, disease-resistance screening, and allele introgression. A structured comparison of major review and protocol papers identifies broad consensus on core parameters alongside genuine divergence on far-red light supplementation. Critical evaluation addresses genotype-by-environment interaction, incomplete trait coverage, infrastructure costs, and unresolved questions on the biological integrity of rapidly advanced generations. The review further discusses new genomic techniques (NGTs), particularly CRISPR/Cas9-based gene editing, in the context of the EU’s June 2026 NGT regulation, under which Category 1 plants carrying only targeted endogenous modifications are exempt from GMO authorisation. When combined with SB, this regulatory shift can compress the interval from gene-editing event to registered variety from decades to a few years. Speed breeding, NGTs, and conventional field-based selection are most productively treated as complementary elements of a unified pipeline.

Summary: Considering that agricultural production needs to be adjusted to global climate changes, it is of particular importance to develop bread wheat germplasm with improved tolerance to abiotic stress conditions. Therefore, the aim of this research was to identify stable wheat genotypes with increased salinity stress tolerance. The experiment was conducted with 27 wheat genotypes, at two localities: Kumane (solonetz soil type) and Rimski Šančevi (chernozem soil type) during two growing seasons. A significant influence of genotype and environment, as well as G × E interaction, on the phenotypic expression of yield components was found. The factor of genotype had the largest effect on the variation in plant height (38.7%) and the smallest effect on the variation in the number of grains per spike (8.24%). The greatest influence of the environment (64.7%) and G × E interaction (17.44%) was found in the variation in the number of grains per spike. Salinity conditions contributed the most to the decrease in the number of grains per spike (30%), which is considered the best indicator of the impact of stress on the plant. The smallest decrease in the number of grains per spike was recorded in the genotypes Šumadija (16.0%) and Harmonija (18.8%). However, AMMI and PCA analyses showed that the genotype Harmonija is characterized by a higher value of the number of grains per spike and greater stability. The genotypes Renesansa, Jugoslavija, Bankut 1205, and Harmonija were characterized by the smallest reduction in plant height under salinity conditions, among which Jugoslavija and Renesansa exhibited high stability and lower trait values. The smallest reduction in spike length was found in the genotypes Jugoslavija and Šumadija, which exhibited the specific adaptability to salinity stress. In terms of salinity tolerance, the genotypes Šumadija, Harmonija, and Renesansa are considered valuable genetic resources in breeding programs.

Quince (Cydonia oblonga Mill.) is a well-known pome fruit species, primarily used for the production of jams and marmalades as well as for pear rootstocks. It has long been cultivated in Serbia at various locations and it possesses vast genetic variability. The aim of this study was to evaluate genetic diversity of 36 genotypes collected from the Fruška Gora locality. Seven SSR markers were used for genetic diversity analyses, revealing a total of 85.7 % polymorphic loci. Six of them were polymorphic, while marker Hi02d04 was homozygous. A total of 20 alleles were recorded, with an average number of alleles per marker of Na = 1.58. The number of effective alleles was Ne = 1.4, observed heterozygosity was Ho = 0.34, while expected heterozygosity was He = 0.22. Principle coordinates analyses (PCoA) explained 74.60% of total diversity with its 3 coordinates and formed 4 different groups of genotypes. The dendrogram constructed with the UPGMA method placed genotypes in 3 different clusters, with variation observed within clusters. The use of SSR markers revealed significant differences between the tested genotypes, providing a closer insight into the state of the genetic resources of this pome species in our country.