The Iberian Peninsula is a key region for unraveling human settlement histories of Eurasia during the period spanning the decline of Neandertals and the emergence of anatomically modern humans (AMH). There is no evidence of human occupation in central Iberia after the disappearance of Neandertals ~42,000 years ago until approximately 26,000 years ago, rendering the region “nobody’s land” during the Aurignacian period. The Abrigo de la Malia provides irrefutable evidence of human settlements dating back to 36,200 to 31,760 calibrated years before the present (cal B.P.) This site also records additional levels of occupation around 32,420 to 26,260 cal B.P., suggesting repeated settlement of this territory. Our multiproxy examination identifies a change in climate trending toward colder and more arid conditions. However, this climatic deterioration does not appear to have affected AMH subsistence strategies or their capacity to inhabit this region. These findings reveal the ability of AMH groups to colonize regions hitherto considered uninhabitable, reopening the debate on early Upper Paleolithic population dynamics of southwestern Europe.

Hydrogen stable isotopes (δ ² H) preserved in bone collagen have been proposed as a promising proxy for palaeoclimatic reconstruction, though empirical applications remain limited. To evaluate the reliability and scope of this approach, we performed a multiisotopic analysis (δ ¹³ C, δ ¹⁵ N, δ ³⁴ S, and δ ² H) on 50 herbivore bone samples collected from three archaeostratigraphic layers spanning the Aurignacian sequence of Isturitz Cave (France). This archaeological well-preserved, dated and studied site provides a robust framework for testing isotopic proxies across temporal and taxonomic gradients. Our results reveal a progressive δ ² H depletion over ∼3000-year interval, consistent with predicted increasing aridity. We also identified species-specific δ ² H differences, underscoring the need for taxonomic resolution in future applications and in general palaeoclimatic interpretations. While these samples reflect faunal remains accumulated through human activity—introducing potential ecological and sampling biases—they nonetheless provide valuable records of past local conditions. Overall, our findings support the potential of bone collagen δ ² H as a sensitive indicator of past hydrological conditions and a complementary tool for reconstructing both climatic trends and humanenvironment dynamics.

During the Upper Paleolithic of southwestern France, the working of mammoth ivory is widely documented, but the use of mammoth bone has not been identified except for one possible Gravettian case. Here we report six worked objects made of mammoth bone from five sites in southwestern France, which have been identified taxonomically by proteomics using ZooMS. They were radiocarbon dated and their chronological distribution spans the Upper Paleolithic: Aurignacian (Sous-le-Roc, 35.5–34.6 ka cal BP), Gravettian (Brassempouy, 28.6–27.6 ka cal BP), Solutrean (Laugerie-Haute and Harpons, 26.3–23.4 and 24.2–23.7 ka cal BP), and Magdalenian (La Madeleine, 15.9–15.5 ka cal BP). Additionally, the technical use of unworked flakes from mammoth bones was identified at another site (Isturitz) and dated to the Gravettian (33.8–31.9 ka cal BP). We argue that in the Upper Paleolithic of southwestern Europe, the use of mammoth bone, while infrequent, is more common than previously documented, and identification biases account for its under-recognition. More systematic research is necessary to assess the extent of this phenomenon. This contribution highlights the research potential in collections from ancient excavations, provided that they are approached with a combination of complementary analytical methods.