The peculiarities of the in vitro regeneration of the rare species L. from floral explants have been studied for the first time. Upon the establishment of in vitro culture and during the multiplication stage, the most effective is the B5 nutrient medium supplemented with 0.44 µM BAP, 3.22 µM NAA, and 2.28 µM IAA. During the multiplication stage, the regeneration rate reaches 80% and the number of bulblets per explant is 3.9 ± 0.3. It is established that the morphogenesis of de novo formed structures depends on the mineral composition of the medium: the use of mineral-based B leads to adventitious shoot formation (gemmogenesis), while the use of BDS causes morphogenic callus formation and the gemmorhizogenesis on its surface. The stimulating effect of low temperatures (+7°C) on bulblet development and adaptation to ex vitro conditions is noted.

Features of in vitro regeneration of Fritillaria sonnikovaefrom bulb scales were studied. The initi-ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM...

Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was assessed on a point scale. When assessing the primers, the number of reproducible amplified DNA fragments, the clarity and brightness of the obtained fragments, and only distinctive bands were taken into account. As a result, 10 ISSR markers were selected that are the most informative for assessing the population diversity of the species.

Сохранение эндемика Западного Саяна Fritillaria sonnikovae Schaulo et A. Erst (Liliaceae) в коллекции in vitro