A voltammetric method was used to estimate the complexing capacity of water extracts from both desert soils sampled at the root zone of creosote and salt cedar plants, and in soils from interspace or background regions where no vegetative influence was apparent. The copper complexing capacity of water extracts of these desert soils was influenced by contact time and pH. In soils from the root zones of creosote and salt cedar plant, copper complexation capacities at pH 8 were from 5 µM to 60 µM after five min contact periods, while 18 h contact periods yielded copper complexation capacities of 40 µM–80 µM. Soils with no vegetative influence had copper complexing capacities of less the 2 µM. The copper complexing capacities of these soils are well correlated with the concentration of organic carbon in the water extract (r² = 0.86). The abundance of soluble organic matter in the root zone of desert shrubs has the potential to control the solution speciation of Cu²⁺. The formation of soluble complexes should also have an important influence on the plant uptake and transport of copper, as well as other heavy metals in the root zones of desert shrubs and beyond.

In soil and water, metal complexation by humic substances has been demonstrated to have great importance in determining the bioavailability of many trace metals including lead. The binding of lead by humic acids has important implications for lead toxicity, and remediation. In this study we demonstrate the use of 4-(2-pyridylazo)-resorcinol (PAR) as a competitive ligand for the purposed of determining sorption isotherms for Pb²⁺ on humic acids and humic acid analogs. Equilibration rates at pH 8 were very rapid and the sorption isotherms measured were fit to the Langmuir isotherm equation and values for the stability constants (K_HA) and complexing site concentrations (L_t) are reported. At a PAR concentration of 0.24 mM and humic acid concentrations of 25-200 mg/L, the mass normalized log K_HA values for the humic substances ranged from 7.2 to 7.9, while the log L_t values ranged from -2.8 to -3.8. At lower PAR concentrations both K_HA and L_t tended to increase in magnitude indicating the role of the PAR concentration in establishing the measurement window for the interaction of Pb²⁺ with humic materials.