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Rice is a staple food for humans and a key source of soil arsenic (As) transfer to the human food chain. As a result, it is critical to develop ways for reducing As accumulation in rice. A pot experiment with seven different water management practices was done to examine the impacts of water management on rice (cv. BRRI dhan28) growth, yield, and As accumulation in rice grain. Any health risk due to As accumulation in rice grain was also determined. The soil used in the experiment was artificially contaminated with As and the source of As was sodium arsenate (Na2HAsO4 7H2O). Water management practices affect different plant growth and yield parameters including filled grains per panicle, unfilled grains per panicle, 1000-grain weight, grain yield and straw yield of rice. The number of filled grains per panicle and 1000-grain weight were found to be at their highest in the T7 (alternate wetting and drying) condition, whereas the number of unfilled grains per panicle was at its lowest in the same treatment. The T7 also demonstrated the highest grain yield (21.08 g/pot) and straw yield (22.02 g/pot), whereas the lowest values were noted in T1 (flooding throughout the growth period). The highest As concentration in rice grain (0.52 mg kg−1) was found in T1 and the lowest As concentration in grain (0.27 mg kg−1) was found in T7. Estimation of the human health risk revealed that the non-carcinogenic risks (HQ > 1) and carcinogenic risks (CR > 1.0 × 10−4) were greatly affected by different water regimes. The rice plant grown under alternate wetting and drying condition (T7) showed the lowest health risks compared to other water management practices. Thus, alternate wetting and drying conditions are a good water management strategy for increasing rice output while reducing arsenic buildup in rice grain.
Israt Jahan Harine; Mohammad Rafiqul Islam; Mahmud Hossain; Hasina Afroz; Rounok Jahan; Abu Bakkar Siddique; Shihab Uddin; Mohammad Anwar Hossain; Saud Alamri; Manzer H. Siddiqui; Robert J. Henry. Arsenic Accumulation in Rice Grain as Influenced by Water Management: Human Health Risk Assessment. Agronomy 2021, 11, 1741 .
AMA StyleIsrat Jahan Harine, Mohammad Rafiqul Islam, Mahmud Hossain, Hasina Afroz, Rounok Jahan, Abu Bakkar Siddique, Shihab Uddin, Mohammad Anwar Hossain, Saud Alamri, Manzer H. Siddiqui, Robert J. Henry. Arsenic Accumulation in Rice Grain as Influenced by Water Management: Human Health Risk Assessment. Agronomy. 2021; 11 (9):1741.
Chicago/Turabian StyleIsrat Jahan Harine; Mohammad Rafiqul Islam; Mahmud Hossain; Hasina Afroz; Rounok Jahan; Abu Bakkar Siddique; Shihab Uddin; Mohammad Anwar Hossain; Saud Alamri; Manzer H. Siddiqui; Robert J. Henry. 2021. "Arsenic Accumulation in Rice Grain as Influenced by Water Management: Human Health Risk Assessment." Agronomy 11, no. 9: 1741.
Melatonin (Mel) and hydrogen sulphide (H2S) have emerged as potential regulators of plant metabolism during abiotic stress. Presence of excess NaCl in the soil is one of the main causes of reduced crop productivity worldwide. The present investigation examines the role of exogenous Mel and endogenous H2S in tomato seedlings grown under NaCl stress. Effect of 30 µm Mel on endogenous synthesis of H2S was examined in roots of NaCl-stressed (200 mm) tomato seedlings. Also, the impact of treatments on the oxidative stress markers, transport of K+ and Na+, and activity of H+-ATPase and antioxidant enzymes was assessed. Results show that NaCl-stressed seedlings supplemented with 30 µm Mel had increased levels of endogenous H2S through enhanced L-cysteine desulfhydrase activity. Mel in association with H2S overcame the deleterious effect of NaCl and induced retention of K+ that maintained a higher K+/Na+ ratio. Use of plasma membrane inhibitors and an H2S scavenger revealed that Mel-induced regulation of K+/Na+ homeostasis in NaCl-stressed seedling roots operates through endogenous H2S signalling. Synergistic effects of Mel and H2S also reduced the generation of ROS and oxidative destruction through the enhanced activity of antioxidant enzymes. Thus, it is suggested that the protective function of Mel against NaCl stress operates through an endogenous H2S-dependent pathway, wherein H+-ATPase-energized secondary active transport regulates K+/Na+ homeostasis.
M. H. Siddiqui; M. N. Khan; S. Mukherjee; R. A. Basahi; S. Alamri; A. A. Al‑Amri; Q. D. Alsubaie; H. M. Ali; B. M. A. Al‐Munqedhi; I. A. A. Almohisen. Exogenous melatonin‐mediated regulation of K + /Na + transport, H + ‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots. Plant Biology 2021, 1 .
AMA StyleM. H. Siddiqui, M. N. Khan, S. Mukherjee, R. A. Basahi, S. Alamri, A. A. Al‑Amri, Q. D. Alsubaie, H. M. Ali, B. M. A. Al‐Munqedhi, I. A. A. Almohisen. Exogenous melatonin‐mediated regulation of K + /Na + transport, H + ‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots. Plant Biology. 2021; ():1.
Chicago/Turabian StyleM. H. Siddiqui; M. N. Khan; S. Mukherjee; R. A. Basahi; S. Alamri; A. A. Al‑Amri; Q. D. Alsubaie; H. M. Ali; B. M. A. Al‐Munqedhi; I. A. A. Almohisen. 2021. "Exogenous melatonin‐mediated regulation of K + /Na + transport, H + ‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots." Plant Biology , no. : 1.
Exogenous potassium (K + ) and endogenous hydrogen sulfide (H 2 S) synergistically alleviate drought stress through regulating H + -ATPase activity, sugar metabolism and redox homoeostasis in tomato seedlings . Present work evaluates the role of K+ in the regulation of endogenous H2S signaling in modulating the tolerance of tomato (Solanum lycopersicum L. Mill.) seedlings to drought stress. The findings reveal that exposure of seedlings to 15% (w/v) polyethylene glycol 8000 (PEG) led to a substantial decrease in leaf K+ content which was associated with reduced H+-ATPase activity. Treatment with sodium orthovanadate (SOV, PM H+-ATPase inhibitor) and tetraethylammonium chloride (TEA, K+ channel blocker) suggests that exogenous K+ stimulated H+-ATPase activity that further regulated endogenous K+ content in tomato seedlings subjected to drought stress. Moreover, reduction in H+-ATPase activity by hypotaurine (HT; H2S scavenger) substantiates the role of endogenous H2S in the regulation of H+-ATPase activity. Elevation in endogenous K+ content enhanced the biosynthesis of H2S through enhancing the synthesis of cysteine, the H2S precursor. Synergistic action of H2S and K+ effectively neutralized drought stress by regulating sugar metabolism and redox homoeostasis that resulted in osmotic adjustment, as witnessed by reduced water loss, and improved hydration level of the stressed seedlings. The integrative role of endogenous H2S in K+ homeostasis was validated using HT and TEA which weakened the protection against drought stress induced impairments. In conclusion, exogenous K+ and endogenous H2S regulate H+-ATPase activity which plays a decisive role in the maintenance of endogenous K+ homeostasis. Thus, present work reveals that K+ and H2S crosstalk is essential for modulation of drought stress tolerance in tomato seedlings.
Manzer H. Siddiqui; M. Nasir Khan; Soumya Mukherjee; Saud Alamri; Riyadh A. Basahi; Abdullah A. Al-Amri; Qasi D. Alsubaie; Bander M. A. Al-Munqedhi; Hayssam M. Ali; Ibrahim A. A. Almohisen. Hydrogen sulfide (H2S) and potassium (K+) synergistically induce drought stress tolerance through regulation of H+-ATPase activity, sugar metabolism, and antioxidative defense in tomato seedlings. Plant Cell Reports 2021, 40, 1543 -1564.
AMA StyleManzer H. Siddiqui, M. Nasir Khan, Soumya Mukherjee, Saud Alamri, Riyadh A. Basahi, Abdullah A. Al-Amri, Qasi D. Alsubaie, Bander M. A. Al-Munqedhi, Hayssam M. Ali, Ibrahim A. A. Almohisen. Hydrogen sulfide (H2S) and potassium (K+) synergistically induce drought stress tolerance through regulation of H+-ATPase activity, sugar metabolism, and antioxidative defense in tomato seedlings. Plant Cell Reports. 2021; 40 (8):1543-1564.
Chicago/Turabian StyleManzer H. Siddiqui; M. Nasir Khan; Soumya Mukherjee; Saud Alamri; Riyadh A. Basahi; Abdullah A. Al-Amri; Qasi D. Alsubaie; Bander M. A. Al-Munqedhi; Hayssam M. Ali; Ibrahim A. A. Almohisen. 2021. "Hydrogen sulfide (H2S) and potassium (K+) synergistically induce drought stress tolerance through regulation of H+-ATPase activity, sugar metabolism, and antioxidative defense in tomato seedlings." Plant Cell Reports 40, no. 8: 1543-1564.
Salt accumulation in soils poses severe challenges for crop production in arid and semi-arid regions. Scarcity of rainfall and a high evaporation rate in these regions are considered major reasons for salt accumulation. It drastically reduces the leaching of excessive salts below the root zone of crops. The toxic effects of salts on plants can be greatly reduced with the use of biological and inorganic amendments. The present study was conducted to investigate the positive influence of gypsum (GP), composted cow dung (CCD) and the combined use of gypsum and composted cow dung (GP+CCD) on the growth, seed yield, and physiological and chemical attributes of sunflowers (Helianthus annuus) in salty soil conditions. Saline-sodic soil was prepared using salts that include NaCl, Na2SO4, MgSO4, and CaCl2. It contained three levels of electrical conductivity (EC), i.e., 1.8, 6, and 12 dS m−1, and had a sodium adsorption ratio (SAR) of 15. We noted significant deleterious effects of excessive salt stress on multiple attributes of the growth, produce, physiology, and chemical factors of sunflowers. However, treatment with GP+CCD improved all these attributes in all these conditions over the control treatment. Treatment with GP+CCD also significantly increased N, P and K contents over the control in the absence of salt stress, i.e., normal conditions. Conversely, treatment with GP+CCD caused an extreme decline in antioxidant enzyme activity (APX, GPX, CAT and SOD) and Na+/K+ ratio in seeds of up to 90, 75, and 71% over control at an EC level of 1.8, 6, and 12 dS m−1, respectively. This study suggests the combined application of gypsum and composted cow dung for better production of sunflowers in salt-affected soils, and augmented growth, yield, physiology, biochemistry and nutritional value in the sunflower seeds.
Muhammad Naveed; Muhammad Aslam; Zulfiqar Ahmad; Tasawar Abbas; Asma Al-Huqail; Manzer Siddiqui; Hayssam Ali; Irfan Ashraf; Adnan Mustafa. Growth Responses, Physiological Alterations and Alleviation of Salinity Stress in Sunflower (Helianthus annuus L.) Amended with Gypsum and Composted Cow Dung. Sustainability 2021, 13, 6792 .
AMA StyleMuhammad Naveed, Muhammad Aslam, Zulfiqar Ahmad, Tasawar Abbas, Asma Al-Huqail, Manzer Siddiqui, Hayssam Ali, Irfan Ashraf, Adnan Mustafa. Growth Responses, Physiological Alterations and Alleviation of Salinity Stress in Sunflower (Helianthus annuus L.) Amended with Gypsum and Composted Cow Dung. Sustainability. 2021; 13 (12):6792.
Chicago/Turabian StyleMuhammad Naveed; Muhammad Aslam; Zulfiqar Ahmad; Tasawar Abbas; Asma Al-Huqail; Manzer Siddiqui; Hayssam Ali; Irfan Ashraf; Adnan Mustafa. 2021. "Growth Responses, Physiological Alterations and Alleviation of Salinity Stress in Sunflower (Helianthus annuus L.) Amended with Gypsum and Composted Cow Dung." Sustainability 13, no. 12: 6792.
Potassium (K+) is one of the vital macronutrients required by plants for proper growth and blossoming harvest. In addition, K+ also plays a decisive role in promoting tolerance to various stresses. Under stressful conditions, plants deploy their defense system through various signaling molecules, including hydrogen sulfide (H2S). The present investigation was carried out to unravel the role of K+ and H2S in plants under NaCl stress. The results of the study show that NaCl stress caused a reduction in K+ and an increase in Na+ content in the tomato seedling roots which coincided with a lower H+-ATPase activity and K+/Na+ ratio. However, application of 5 mM K+, in association with endogenous H2S, positively regulated the Na+/H+ antiport system that accelerated K+ influx and Na+ efflux, resulting in the maintenance of a higher K+/Na+ ratio. The role of K+ and H2S in the regulation of the Na+/H+ antiport system was validated by applying sodium orthovanadate (plasma membrane H+-ATPase inhibitor), tetraethylammonium chloride (K+ channel blocker), amiloride (Na+/H+ antiporter inhibitor), and hypotaurine (HT, H2S scavenger). Application of 5 mM K+ positively regulated the ascorbate–glutathione cycle and activity of antioxidant enzymes that resulted in a reduction in reactive oxygen species generation and associated damage. Under NaCl stress, K+ also activated carbohydrate metabolism and proline accumulation that caused improvement in osmotic tolerance and enhanced the hydration level of the stressed seedlings. However, inclusion of the H2S scavenger HT reversed the effect of K+, suggesting H2S-dependent functioning of K+ under NaCl stress. Therefore, the present findings report that K+, in association with H2S, alleviates NaCl-induced impairments by regulating the Na+/H+ antiport system, carbohydrate metabolism, and antioxidative defense system.
M. Khan; Soumya Mukherjee; Asma Al-Huqail; Riyadh Basahi; Hayssam Ali; Bander Al-Munqedhi; Manzer Siddiqui; Hazem Kalaji. Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots. Plants 2021, 10, 948 .
AMA StyleM. Khan, Soumya Mukherjee, Asma Al-Huqail, Riyadh Basahi, Hayssam Ali, Bander Al-Munqedhi, Manzer Siddiqui, Hazem Kalaji. Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots. Plants. 2021; 10 (5):948.
Chicago/Turabian StyleM. Khan; Soumya Mukherjee; Asma Al-Huqail; Riyadh Basahi; Hayssam Ali; Bander Al-Munqedhi; Manzer Siddiqui; Hazem Kalaji. 2021. "Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots." Plants 10, no. 5: 948.
In this study, we have explored the possible role of ascorbic acid (ASC) and glutathione (GSH) in alleviating arsenate (AsV) toxicity in brinjal roots. Moreover, we have also focused our attention on the possible involvement of endogenous nitric oxide (NO) in accomplishing this task. Arsenate treatment negatively impacts the length and fresh weight of roots and shoots as well as the dry weight and fitness of roots, and this was accompanied by greater As accumulation in roots and shoots of brinjal. Arsenate treatment also declined the endogenous NO level by inhibiting NITRIC OXIDE SYNTHASE‐LIKE (NOS‐like) activity. Furthermore, AsV stimulated oxidative stress markers, caused protein damage by their carbonylation due to down‐regulation in antioxidants (particularly ascorbate‐glutathione cycle), leading to disturbed cellular redox status. This, collectively, led to root cell death in brinjal. However, the addition of either ASC or GSH rescued brinjal roots from the toxic effects of AsV in. Interestingly, lycorine (an inhibitor of ASC biosynthesis) further increased AsV toxicity, while ASC rescued its effects. Moreover, buthionine sulphoximine (BSO, an inhibitor of glutathione biosynthesis) interestingly increased further AsV toxicity, while GSH rescued the plant from the As toxic effects. An interesting notion of this study was that GSH rescued the toxic effect of lycorine, while ASC rescued the toxic effect of BSO, though the AsV toxicity mediated by either ASC or GSH was always accompanied by high endogenous NO level and NOS‐like activity. All together, these results suggest that ASC and GSH independently mitigate AsV toxicity in brinjal roots, but both might be dependent on endogenous NO for accomplishing the AsV toxicity alleviatory tasks.
Saud Alamri; Bishwajit Kumar Kushwaha; Vijay Pratap Singh; Manzer H. Siddiqui; Abdullah A. Al‐Amri; Qasi D. Alsubaie; Hayssam M. Ali. Ascorbate and glutathione independently alleviate arsenate toxicity in brinjal but both require endogenous nitric oxide. Physiologia Plantarum 2021, 1 .
AMA StyleSaud Alamri, Bishwajit Kumar Kushwaha, Vijay Pratap Singh, Manzer H. Siddiqui, Abdullah A. Al‐Amri, Qasi D. Alsubaie, Hayssam M. Ali. Ascorbate and glutathione independently alleviate arsenate toxicity in brinjal but both require endogenous nitric oxide. Physiologia Plantarum. 2021; ():1.
Chicago/Turabian StyleSaud Alamri; Bishwajit Kumar Kushwaha; Vijay Pratap Singh; Manzer H. Siddiqui; Abdullah A. Al‐Amri; Qasi D. Alsubaie; Hayssam M. Ali. 2021. "Ascorbate and glutathione independently alleviate arsenate toxicity in brinjal but both require endogenous nitric oxide." Physiologia Plantarum , no. : 1.
Wheat is one of the leading staple crops in many countries. Phosphorus (P) plays an important role for wheat growth and yield as it takes part in many metabolic pathways. Even for soluble phosphatic fertilizers, most of the Pakistani soils, being alkaline and calcareous in nature, show phosphorus use efficiency (PUE) not more than 10–25%. The major issue is the unavailability of P due to fixation and precipitation reactions with soil particles. Composting of rock-phosphate with animal and poultry manures supplied with bio-stimulated phosphate solubilizing bacteria (PSB) not only enhances the RP solubilization but also serves as a potent source of P for plants. Composted/bio-activated rock-phosphate (B-RP), prepared by group of three bacterial strains i.e., Pseudomonas sp. (E11), Bacillus sp. (MN54) and Enterobacter sp. (MN17) aided with molasses (5%) and urea (10%), was tested alone and in various combinations with di-ammonium phosphate (DAP). In this pot trial, the combined application of B-RP and DAP was found superior to the sole application of B-RP. Even the combination of B-RP and DAP sharing equal amount of recommended P showed better results as compared to the sole application of DAP, giving improved shoot biomass (25%), total P-uptake (67%), recovery efficiency of P (75%), dry matter (29%), crude protein (29%), and other yield, physiological and nutritional quality parameters of wheat. So, it could be concluded that integrated use of B-RP and DAP with equal proportion of recommended P could serve as a better management practice for not only improving quantity but also the quality of the wheat grain.
Muhammad Arfan-Ul-Haq; Muhammad Yaseen; Muhammad Naveed; Adnan Mustafa; Sulman Siddique; Saud Alamri; Manzer Siddiqui; Abdullah Al-Amri; Qasi D. Alsubaie; Hayssam Ali. Deciphering the Potential of Bioactivated Rock Phosphate and Di-Ammonium Phosphate on Agronomic Performance, Nutritional Quality and Productivity of Wheat (Triticum aestivum L.). Agronomy 2021, 11, 684 .
AMA StyleMuhammad Arfan-Ul-Haq, Muhammad Yaseen, Muhammad Naveed, Adnan Mustafa, Sulman Siddique, Saud Alamri, Manzer Siddiqui, Abdullah Al-Amri, Qasi D. Alsubaie, Hayssam Ali. Deciphering the Potential of Bioactivated Rock Phosphate and Di-Ammonium Phosphate on Agronomic Performance, Nutritional Quality and Productivity of Wheat (Triticum aestivum L.). Agronomy. 2021; 11 (4):684.
Chicago/Turabian StyleMuhammad Arfan-Ul-Haq; Muhammad Yaseen; Muhammad Naveed; Adnan Mustafa; Sulman Siddique; Saud Alamri; Manzer Siddiqui; Abdullah Al-Amri; Qasi D. Alsubaie; Hayssam Ali. 2021. "Deciphering the Potential of Bioactivated Rock Phosphate and Di-Ammonium Phosphate on Agronomic Performance, Nutritional Quality and Productivity of Wheat (Triticum aestivum L.)." Agronomy 11, no. 4: 684.
Potassium (K) is an essential macronutrient which is known to regulate key metabolic processes, modulate enzyme activity and plays a decisive role in osmotic adjustment in plants. Present work evaluates the role of K in the regulation of endogenous hydrogen sulfide (H2S) signaling in modulating the tolerance of tomato (Solanum lycopersicum L. Mill.) seedlings to osmotic stress. The findings reveal that exposure of seedlings to 15% (w/v) polyethylene glycol 8000 (PEG) led to a substantial decrease in leaf K content which was associated with reduced H+-ATPase activity. Exogenous application of K to the stressed seedlings significantly improved endogenous K content. Treatment with sodium orthovanadate (SOV, PM H+-ATPase inhibitor) and tetraethylammonium chloride (TEA, K channel blocker) suggests that exogenous K stimulated H+-ATPase activity that further regulated endogenous K content in tomato seedlings subjected to osmotic stress. Moreover, reduction in H+-ATPase activity by hypotaurine (H2S scavenger) substantiates the role of endogenous H2S in the regulation of H+-ATPase activity. Elevation in endogenous K content enhanced the biosynthesis of hydrogen sulfide (H2S) through enhancing the synthesis of cysteine, the H2S precursor. Synergistic action of H2S and K effectively neutralized osmotic stress by regulating sugar metabolism that resulted in osmotic adjustment, as witnessed by reduced water loss and improved hydration level of the stressed seedlings. Cross talk of H2S and K also assisted the seedlings in the activation of antioxidant enzymes that controlled the generation of reactive oxygen species and led to the protection against oxidative stress. The integrative role of H2S and K signaling was validated using hypotaurine (H2S scavenger) and TEA (K channel blocker) which weakened the protection against osmotic stress induced impairments. In conclusion, exogenous K and endogenous H2S regulate H+-ATPase activity which plays a decisive role in the maintenance of endogenous K homeostasis. Thus, present work reveals that K and H2S crosstalk is essential for modulation of osmotic stress tolerance in tomato seedlings.
Manzer H. Siddiqui; Soumya Mukherjee; Saud Alamri; Yanbo Hu; Abdullah Alamri; Qasi Alsubaie; Bander Al-Munqedhi; Hayssam Ali. Hydrogen sulfide and potassium synergistically induce osmotic stress tolerance through regulation of H+-ATPase activity and sugar metabolism in tomato seedlings. 2021, 1 .
AMA StyleManzer H. Siddiqui, Soumya Mukherjee, Saud Alamri, Yanbo Hu, Abdullah Alamri, Qasi Alsubaie, Bander Al-Munqedhi, Hayssam Ali. Hydrogen sulfide and potassium synergistically induce osmotic stress tolerance through regulation of H+-ATPase activity and sugar metabolism in tomato seedlings. . 2021; ():1.
Chicago/Turabian StyleManzer H. Siddiqui; Soumya Mukherjee; Saud Alamri; Yanbo Hu; Abdullah Alamri; Qasi Alsubaie; Bander Al-Munqedhi; Hayssam Ali. 2021. "Hydrogen sulfide and potassium synergistically induce osmotic stress tolerance through regulation of H+-ATPase activity and sugar metabolism in tomato seedlings." , no. : 1.
Essential micronutrients for plants (some of which are metals) are necessary to regulate many routine processes in plants, their excess, however, can have detrimental consequences and can hamper the plant growth, physiology, and metabolism. In this investigation, the physio‐biochemical mechanisms involved in exogenous hydrogen sulfide‐mediated (H2S) alleviation of copper (Cu) stress in Artemisia annua were assessed. The two different levels of Cu (20, 40 mg kg‐1), one H2S treatment (200 µM) and their combinations were introduced into the soil, while one set of plants was kept as control. Results suggested that presence of Cu in the soil reduced growth and biomass, photosynthetic parameters, chlorophyll content and fluorescence, gas exchange parameters and induced antioxidant enzyme activities. Copper stress enhanced the production of TBARS content and increased Cu content in root and shoot tissues of affected plants. Exogenous application of H2S enhanced the physio‐biochemical characteristics of Cu‐treated A. annua plants. Hydrogen sulfide reduced the lipid peroxidation by enhancing the activity of antioxidant enzymes in Cu‐stressed plants as compared with their respective controls. Hydrogen sulfide also reduced the Cu content in different parts of the plant, whereas increased the photosynthetic efficiency, trichome density, average area of trichomes and artemisinin content. Therefore, our result provides a comprehension into the defensive role of H2S in Cu‐stressed A. annua plants.
Laibah Nomani; Andleeb Zehra; Sadaf Choudhary; Kaiser Iqbal Wani; M. Naeem; Manzer H. Siddiqui; M. Masroor A. Khan; Tariq Aftab. Exogenous hydrogen sulphide alleviates copper stress impacts in Artemisia annua L.: Growth, antioxidant metabolism, glandular trichome development and artemisinin biosynthesis. Plant Biology 2021, 1 .
AMA StyleLaibah Nomani, Andleeb Zehra, Sadaf Choudhary, Kaiser Iqbal Wani, M. Naeem, Manzer H. Siddiqui, M. Masroor A. Khan, Tariq Aftab. Exogenous hydrogen sulphide alleviates copper stress impacts in Artemisia annua L.: Growth, antioxidant metabolism, glandular trichome development and artemisinin biosynthesis. Plant Biology. 2021; ():1.
Chicago/Turabian StyleLaibah Nomani; Andleeb Zehra; Sadaf Choudhary; Kaiser Iqbal Wani; M. Naeem; Manzer H. Siddiqui; M. Masroor A. Khan; Tariq Aftab. 2021. "Exogenous hydrogen sulphide alleviates copper stress impacts in Artemisia annua L.: Growth, antioxidant metabolism, glandular trichome development and artemisinin biosynthesis." Plant Biology , no. : 1.
Plant growth regulators and Rhizobium are actively involved in the regulation of flowering, pod formation, nodulation, and ultimately the growth and yield of legumes. However, very limited information is available on the combined effect of gibberellic acid (GA3) and Rhizobium on growth attributes and yield of legume crops. This experiment was designed to fill this gap by studying the performance of chickpea under exogenous application of GA3 (10−4 and 10−5 M) alone and in combination with Rhizobium. Exogenous application of GA3 (10−5 M) combined with rhizobium inoculation gave the highest values for number of nodules per plant (16) and their dry biomass (0.22 g). Moreover, GA3 application and seed inoculation with Rhizobium, when applied singly, significantly enhanced chickpea growth. However, the most promising results were obtained by the inoculation of Rhizobium accompanied with GA3 (10−5 M). Plant height, grain and stover yield, and chlorophyll contents were enhanced up to 35%, 39%, 21%, and 51%, respectively. Likewise, the bioaccumulation of macronutrients (N, P and K) was maximum in plants receiving both Rhizobium inoculation and GA3 application. It is concluded that the combined application of Rhizobium and GA3 has synergistic effects on the growth, yield, and nutrient contents of chickpea.
Munazza Rafique; Muhammad Naveed; Adnan Mustafa; Saleem Akhtar; Muneeb Munawar; Sadia Kaukab; Hayssam M. Ali; Manzer H. Siddiqui; Mohamed Z. M. Salem. The Combined Effects of Gibberellic Acid and Rhizobium on Growth, Yield and Nutritional Status in Chickpea (Cicer arietinum L.). Agronomy 2021, 11, 105 .
AMA StyleMunazza Rafique, Muhammad Naveed, Adnan Mustafa, Saleem Akhtar, Muneeb Munawar, Sadia Kaukab, Hayssam M. Ali, Manzer H. Siddiqui, Mohamed Z. M. Salem. The Combined Effects of Gibberellic Acid and Rhizobium on Growth, Yield and Nutritional Status in Chickpea (Cicer arietinum L.). Agronomy. 2021; 11 (1):105.
Chicago/Turabian StyleMunazza Rafique; Muhammad Naveed; Adnan Mustafa; Saleem Akhtar; Muneeb Munawar; Sadia Kaukab; Hayssam M. Ali; Manzer H. Siddiqui; Mohamed Z. M. Salem. 2021. "The Combined Effects of Gibberellic Acid and Rhizobium on Growth, Yield and Nutritional Status in Chickpea (Cicer arietinum L.)." Agronomy 11, no. 1: 105.
Present investigation reports the role of calcium (Ca2+) and hydrogen sulfide (H2S) crosstalk associated with Vigna radiata seedlings subjected to K+ deficient conditions under short-term (24 h) and long-term (72 h) NaCl stress. Perusal of the data reveals that under short-term NaCl stress an initial decline in K+ level led to the elevation in Ca2+ and H2S levels along with improvement in antioxidant system and reduction in reactive oxygen species (ROS) production. Under long-term NaCl stress a further decline in K+ content was deleterious that led to a lower K+/Na+ ratio. This was followed by reduction in antioxidant system along with excessive accumulation of ROS and methylglyoxal content, and increased membrane damage. However, supplementation of the seedling roots with Ca2+ enhanced biosynthesis of H2S through enhancing cysteine pool. The present findings suggest that synergistic action of Ca2+ and H2S induced the activity of H+-ATPase that created H+ gradient which in turn induced Na+/H+ antiport system that accelerated K+ influx and Na+ efflux. All of these together contributed to a higher K+/Na+ ratio, activation of antioxidant defense system, and maintenance of redox homeostasis and membrane integrity in Ca2+-supplemented stressed seedlings. Role of Ca2+ and H2S in the regulation of Na+/H+ antiport system was validated by the use of sodium orthovanadate (plasma membrane H+-ATPase inhibitor), tetraethylammonium chloride (K+ channel blocker), and amiloride (Na+/H+ antiporter inhibitor). Application of Ca2+-chelator EGTA (ethylene glycol-bis(b-aminoethylether)-N,N,Nʹ,Nʹ-tetraacetic acid) and H2S scavenger hypotaurine abolished the effect of Ca2+, suggesting the involvement of Ca2+ and H2S in the alleviation of NaCl stress. Moreover, use of EGTA and HT also substantiates the downstream functioning of H2S during Ca2+-mediated regulation of plant adaptive responses to NaCl stress. To sum up, present findings reveal the association of Ca2+ and H2S signaling in the regulation of ion homeostasis and antioxidant defense during K+-deficient NaCl stress.
M. Nasir Khan; Manzer H. Siddiqui; Soumya Mukherjee; Saud Alamri; Abdullah A. Al-Amri; Qasi D. Alsubaie; Bander M.A. Al-Munqedhi; Hayssam M. Ali. Calcium-hydrogen sulfide crosstalk during K+-deficient NaCl stress operates through regulation of Na+/H+ antiport and antioxidative defense system in mung bean roots. Plant Physiology and Biochemistry 2020, 159, 211 -225.
AMA StyleM. Nasir Khan, Manzer H. Siddiqui, Soumya Mukherjee, Saud Alamri, Abdullah A. Al-Amri, Qasi D. Alsubaie, Bander M.A. Al-Munqedhi, Hayssam M. Ali. Calcium-hydrogen sulfide crosstalk during K+-deficient NaCl stress operates through regulation of Na+/H+ antiport and antioxidative defense system in mung bean roots. Plant Physiology and Biochemistry. 2020; 159 ():211-225.
Chicago/Turabian StyleM. Nasir Khan; Manzer H. Siddiqui; Soumya Mukherjee; Saud Alamri; Abdullah A. Al-Amri; Qasi D. Alsubaie; Bander M.A. Al-Munqedhi; Hayssam M. Ali. 2020. "Calcium-hydrogen sulfide crosstalk during K+-deficient NaCl stress operates through regulation of Na+/H+ antiport and antioxidative defense system in mung bean roots." Plant Physiology and Biochemistry 159, no. : 211-225.
Petroleum hydrocarbon (PHC) contamination of soil is a widespread global environmental concern due to the persistence and recalcitrant nature of PHCs. The PHCs are highly toxic and their removal from the terrestrial ecosystem is necessary to maintain soil as well as human health. Here, a pot experiment was performed to examine the impact of Enterobacter sp. MN17 and biochar addition on the growth of mungbean plants and PHCs removal from diesel-polluted soil. For this purpose, soil was contaminated artificially with diesel to achieve a final concentration of 5000 mg kg−1. Untreated and Enterobacter sp. MN17 treated mungbean seeds were sown in pots. Sugarcane bagasse biochar was applied as an amendment in respective pots along with the recommended levels of essential nutrients. Results showed that PHCs significantly suppressed the seedling emergence as well as agronomic and physiological attributes of mungbean as compared to un-contaminated controls. However, the co-application of Enterobacter sp. MN17 and biochar significantly reduced the phytotoxicity of PHCs to mungbean plants and effectively increased the seedling emergence, shoot and root length, shoot fresh and dry biomass, root fresh and dry biomass of plants up to 24%, 54%, 52%, 52%, 54%, 55% and 60%, respectively as compared to controls. Similarly, 30%, 57%, 64%, 36% and 57% increase in chlorophylls contents, transpiration rate, stomatal conductance, sub-stomatal conductance, and photosynthetic rate, respectively were observed in their combined application as compared to respective controls. Furthermore, the co-addition of biochar and Enterobacter sp. MN17 could remove 69% and 85% higher PHCs from unplanted and planted pots, respectively, than that of their respective controls. Our results suggest that the co-application of biochar and Enterobacter sp. MN17 may be useful in enhancing plant growth and eliminating PHCs from contaminated soil.
Muhammad Hayder Ali; Muhammad Tayyab Sattar; Muhammad Imran Khan; Muhammad Naveed; Munazza Rafique; Saud Alamri; Manzer H. Siddiqui. Enhanced Growth of Mungbean and Remediation of Petroleum Hydrocarbons by Enterobacter sp. MN17 and Biochar Addition in Diesel Contaminated Soil. Applied Sciences 2020, 10, 8548 .
AMA StyleMuhammad Hayder Ali, Muhammad Tayyab Sattar, Muhammad Imran Khan, Muhammad Naveed, Munazza Rafique, Saud Alamri, Manzer H. Siddiqui. Enhanced Growth of Mungbean and Remediation of Petroleum Hydrocarbons by Enterobacter sp. MN17 and Biochar Addition in Diesel Contaminated Soil. Applied Sciences. 2020; 10 (23):8548.
Chicago/Turabian StyleMuhammad Hayder Ali; Muhammad Tayyab Sattar; Muhammad Imran Khan; Muhammad Naveed; Munazza Rafique; Saud Alamri; Manzer H. Siddiqui. 2020. "Enhanced Growth of Mungbean and Remediation of Petroleum Hydrocarbons by Enterobacter sp. MN17 and Biochar Addition in Diesel Contaminated Soil." Applied Sciences 10, no. 23: 8548.
Nickel (Ni) bioavailable fraction in the soil is of utmost importance because of its involvement in plant growth and environmental feedbacks. High concentrations of Ni in the soil environment, especially in the root zone, may retard plant growth that ultimately results in reduced plant biomass and yield. However, endophytic microorganisms have great potential to reduce the toxicity of Ni, especially when applied together with zeolite. The present research work was conducted to evaluate the potential effects of an endophytic bacterium Caulobacter sp. MN13 in combination with zeolite on the physiology, growth, quality, and yield of sesame plant under normal and Ni stressed soil conditions through possible reduction of Ni uptake. Surface sterilized sesame seeds were sown in pots filled with artificially Ni contaminated soil amended with zeolite. Results revealed that plant agronomic attributes such as shoot root dry weight, total number of pods, and 1000-grains weight were increased by 41, 45, 54, and 65%, respectively, over control treatment, with combined application of bacteria and zeolite in Ni contaminated soil. In comparison to control, the gaseous exchange parameters (CO2 assimilation rate, transpiration rate, stomatal- sub-stomatal conductance, chlorophyll content, and vapor pressure) were significantly enhanced by co-application of bacteria and zeolite ranging from 20 to 49% under Ni stress. Moreover, the combined utilization of bacteria and zeolite considerably improved water relations of sesame plant, in terms of relative water content (RWC) and relative membrane permeability (RMP) along with improvement in biochemical components (protein, ash, crude fiber, fat), and micronutrients in normal as well as in Ni contaminated soil. Moreover, the same treatment modulated the Ni-stress in plants through improvement in antioxidant enzymes (AEs) activities along with improved Ni concentration in the soil and different plant tissues. Correlation and principal component analysis (PCA) further revealed that combined application of metal-tolerant bacterium Caulobacter sp. MN13 and zeolite is the most influential strategy in alleviating Ni-induced stress and subsequent improvement in growth, yield, and physio-biochemical attributes of sesame plant.
Muhammad Naveed; Syeda Sosan Bukhari; Adnan Mustafa; Allah Ditta; Saud Alamri; Mohamed A. El-Esawi; Munazza Rafique; Sobia Ashraf; Manzer H. Siddiqui. Mitigation of Nickel Toxicity and Growth Promotion in Sesame through the Application of a Bacterial Endophyte and Zeolite in Nickel Contaminated Soil. International Journal of Environmental Research and Public Health 2020, 17, 8859 .
AMA StyleMuhammad Naveed, Syeda Sosan Bukhari, Adnan Mustafa, Allah Ditta, Saud Alamri, Mohamed A. El-Esawi, Munazza Rafique, Sobia Ashraf, Manzer H. Siddiqui. Mitigation of Nickel Toxicity and Growth Promotion in Sesame through the Application of a Bacterial Endophyte and Zeolite in Nickel Contaminated Soil. International Journal of Environmental Research and Public Health. 2020; 17 (23):8859.
Chicago/Turabian StyleMuhammad Naveed; Syeda Sosan Bukhari; Adnan Mustafa; Allah Ditta; Saud Alamri; Mohamed A. El-Esawi; Munazza Rafique; Sobia Ashraf; Manzer H. Siddiqui. 2020. "Mitigation of Nickel Toxicity and Growth Promotion in Sesame through the Application of a Bacterial Endophyte and Zeolite in Nickel Contaminated Soil." International Journal of Environmental Research and Public Health 17, no. 23: 8859.
At present plants continuously exposed to salinity stress due to the challenging environment that has reduced the crop growth and productivity worldwide. Application of phytohormones by using seed priming method emerges as one of the most reliable and cost effective to alleviate the toxic effect of salinity stress. In this study, we evaluate the effect of seed-primed salicylic acid (SA) to reduce the adverse effect of different salt concentrations (0, 100, 200, and 300 mM NaCl) in pea (Pisum sativum L.) seedlings. After seedling emergence, percent seed germination was calculated; however, after 60 days; plants were sampled for studying the growth and photosynthetic traits, lipid peroxidation level, antioxidant activities, ions accumulation, and its sequestration. The results depicted that salinity treatments hampered overall growth performance and induced oxidative stress in a dose-dependent manner. Salinity also has negatively influence on ion accumulation as Na+ ion increased while K+ ion decreased. On the other hand, seed priming with SA significantly reduced the salinity-induced effects on the overall performance of plants, including growth and photosynthetic attributes. SA alleviated the adverse effect of salinity even at higher salinity level by inducing enzymatic and non-enzymatic antioxidant systems, soluble sugars, and proline accumulation, and regulating ion homeostasis along with up-regulation of Na+/H+ antiporters (SOS1 and NHX1). Thus, seed priming with SA shows a comprehensive role in mitigation of salinity stress and can be used as a model for promising salinity tolerant cultivation.
Farhan Ahmad; Aisha Kamal; Ananya Singh; Farha Ashfaque; Saud Alamri; Manzer H. Siddiqui. Salicylic Acid Modulates Antioxidant System, Defense Metabolites, and Expression of Salt Transporter Genes in Pisum sativum Under Salinity Stress. Journal of Plant Growth Regulation 2020, 1 -14.
AMA StyleFarhan Ahmad, Aisha Kamal, Ananya Singh, Farha Ashfaque, Saud Alamri, Manzer H. Siddiqui. Salicylic Acid Modulates Antioxidant System, Defense Metabolites, and Expression of Salt Transporter Genes in Pisum sativum Under Salinity Stress. Journal of Plant Growth Regulation. 2020; ():1-14.
Chicago/Turabian StyleFarhan Ahmad; Aisha Kamal; Ananya Singh; Farha Ashfaque; Saud Alamri; Manzer H. Siddiqui. 2020. "Salicylic Acid Modulates Antioxidant System, Defense Metabolites, and Expression of Salt Transporter Genes in Pisum sativum Under Salinity Stress." Journal of Plant Growth Regulation , no. : 1-14.
Soil contamination with heavy metals caused by various industrial activities is a threatening global environmental issue of the current era. Chromium (Cr) is the most toxic heavy metal used in leather industry and disposal of untreated wastewater into natural water bodies leads to contamination of natural soil and water resources. We studied the combined effect of biochar and compost on improving the tolerance to Cr toxicity by enhancing the morpho‐physiological and biochemical attributes of two maize cultivars (P‐1543 and NK‐8441) grown in tannery waste polluted soils. The results of this study reveal that Cr toxicity reduced the plant growth by affecting physiological and biochemical attributes. Here, compost and biochar application significantly increased the plant biomass (fresh and dry), height, photosynthesis, chlorophyll content, water relation, starch, and protein content over treatment set as control. However, significant decline in electrolyte leakage (EL), proline, lipid peroxidation, soluble sugars, and antioxidant enzymes (APX, GPX, GR, GST, GSH, SOD, and CAT) was observed by combined application of compost and biochar. Hexavalent chromium concentration was maximum decreased to 4.1 μg g‐1 in soil after post harvesting of maize cultivar NK‐8441, while in roots and shoots to 22.6 and 19.2 μg g‐1 of maize cultivar P‐1543, respectively, by combined application of compost and biochar. Moreover, these both amendments in combination showed considerably better results than their sole application and cultivar P‐1543 comparatively performed better than NK 8441, in both K and S soils. Correlation and principal component analysis (PCA) revealed mostly highly positive associations among all the studied morpho, physio and biochemical attributes of maize plant with the few exceptions, particularly concentration of Cr(III) and Cr(VI) in soil. The present work concluded that combined use of biochar and compost has great potential to decrease Cr toxicity and improve plant growth in tannery polluted soils. This article is protected by copyright. All rights reserved.
Muhammad Asaad Bashir; Muhammad Naveed; Sobia Ashraf; Adnan Mustafa; Qasim Ali; Munazza Rafique; Saud Alamri; Manzer H. Siddiqui. Performance of Zea mays L. cultivars in tannery polluted soils: Management of chromium phytotoxicity through the application of biochar and compost. Physiologia Plantarum 2020, 1 .
AMA StyleMuhammad Asaad Bashir, Muhammad Naveed, Sobia Ashraf, Adnan Mustafa, Qasim Ali, Munazza Rafique, Saud Alamri, Manzer H. Siddiqui. Performance of Zea mays L. cultivars in tannery polluted soils: Management of chromium phytotoxicity through the application of biochar and compost. Physiologia Plantarum. 2020; ():1.
Chicago/Turabian StyleMuhammad Asaad Bashir; Muhammad Naveed; Sobia Ashraf; Adnan Mustafa; Qasim Ali; Munazza Rafique; Saud Alamri; Manzer H. Siddiqui. 2020. "Performance of Zea mays L. cultivars in tannery polluted soils: Management of chromium phytotoxicity through the application of biochar and compost." Physiologia Plantarum , no. : 1.
In the present study, we investigated the impact of different combinations of wheat straw biochar, compost and biogas slurry on maize growth, physiology, and nutritional status in less productive soils. The experiment was performed as a completely randomized block design in a greenhouse pot experiment. The compost and biogas slurry were applied with and without biochar. The results revealed that a combination of biochar, compost, and biogas slurry enhanced the cation exchange capacity (31%), carbon (83%), phosphorus (67%) and potassium (81%) contents in the soil. Likewise, a significant increase in soil microbial biomass carbon (15%) and nitrogen (37%) was noticed with the combined use of all organic amendments. Moreover, the combined application of biochar, compost and biogas slurry enhanced soil urease and β-glucosidase activity up to 96% and 67% over control respectively. In addition, plant height, chlorophyll content, water use efficiency and 1000-grain weight were also enhanced up to 54%, 90%, 53% and 21% respectively, with the combined use of all amendments. Here, biochar addition helped to reduce the nutrient losses of compost and biogas slurry as well. It is concluded that biochar application in combination with compost and biogas slurry could be a more sustainable, environment-friendly and cost-effective approach, particularly for less fertile soils.
Aown Abbas; Muhammad Naveed; Muhammad Azeem; Muhammad Yaseen; Rehmat Ullah; Saud Alamri; Qurrat Ul Ain Farooq; Manzer H. Siddiqui. Efficiency of Wheat Straw Biochar in Combination with Compost and Biogas Slurry for Enhancing Nutritional Status and Productivity of Soil and Plant. Plants 2020, 9, 1516 .
AMA StyleAown Abbas, Muhammad Naveed, Muhammad Azeem, Muhammad Yaseen, Rehmat Ullah, Saud Alamri, Qurrat Ul Ain Farooq, Manzer H. Siddiqui. Efficiency of Wheat Straw Biochar in Combination with Compost and Biogas Slurry for Enhancing Nutritional Status and Productivity of Soil and Plant. Plants. 2020; 9 (11):1516.
Chicago/Turabian StyleAown Abbas; Muhammad Naveed; Muhammad Azeem; Muhammad Yaseen; Rehmat Ullah; Saud Alamri; Qurrat Ul Ain Farooq; Manzer H. Siddiqui. 2020. "Efficiency of Wheat Straw Biochar in Combination with Compost and Biogas Slurry for Enhancing Nutritional Status and Productivity of Soil and Plant." Plants 9, no. 11: 1516.
Plant growth-promoting bacteria (PGPB) and putrescine (Put) have shown a promising role in the mitigation of abiotic stresses in plants. The present study was anticipated to elucidate the potential of Bacillus thuringiensis IAGS 199 and Put in mitigation of cadmium (Cd)-induced toxicity in Capsicum annum. Cadmium toxicity decreased growth, photosynthetic rate, gas exchange attributes and activity of antioxidant enzymes in C. annum seedlings. Moreover, higher levels of protein and non-protein bound thiols besides increased Cd contents were also observed in Cd-stressed plants. B. thuringiensis IAGS 199 and Put, alone or in combination, reduced electrolyte leakage (EL), hydrogen peroxide (H2O2) and malondialdehyde (MDA) level in treated plants. Synergistic effect of B. thuringiensis IAGS 199 and Put significantly enhanced the activity of stress-responsive enzymes including peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD). Furthermore, Put and microbial interaction enhanced the amount of proline, soluble sugars, and total soluble proteins in C. annum plants grown in Cd-contaminated soil. Data obtained during the current study advocates that application of B. thuringiensis IAGS 199 and Put establish a synergistic role in the mitigation of Cd-induced stress through modulating physiochemical features of C. annum plants.
Anis Shah; Fatima Bibi; Iqtidar Hussain; Nasim Yasin; Waheed Akram; Muhammad Tahir; Hayssam Ali; Mohamed Salem; Manzer Siddiqui; Subhan Danish; Shah Fahad; Rahul Datta. Synergistic Effect of Bacillus thuringiensis IAGS 199 and Putrescine on Alleviating Cadmium-Induced Phytotoxicity in Capsicum annum. Plants 2020, 9, 1512 .
AMA StyleAnis Shah, Fatima Bibi, Iqtidar Hussain, Nasim Yasin, Waheed Akram, Muhammad Tahir, Hayssam Ali, Mohamed Salem, Manzer Siddiqui, Subhan Danish, Shah Fahad, Rahul Datta. Synergistic Effect of Bacillus thuringiensis IAGS 199 and Putrescine on Alleviating Cadmium-Induced Phytotoxicity in Capsicum annum. Plants. 2020; 9 (11):1512.
Chicago/Turabian StyleAnis Shah; Fatima Bibi; Iqtidar Hussain; Nasim Yasin; Waheed Akram; Muhammad Tahir; Hayssam Ali; Mohamed Salem; Manzer Siddiqui; Subhan Danish; Shah Fahad; Rahul Datta. 2020. "Synergistic Effect of Bacillus thuringiensis IAGS 199 and Putrescine on Alleviating Cadmium-Induced Phytotoxicity in Capsicum annum." Plants 9, no. 11: 1512.
Salinity is major abiotic stress affecting crop yield, productivity and reduces the land-usage area for agricultural practices. The purpose of this study is to analyze the effect of green-synthesized silver nanoparticle (AgNP) on physiological traits of wheat (Triticum aestivum) under salinity stress. Using augmented and high-throughput characterization of synthesized AgNPs, this study investigated the proximity of AgNPs-induced coping effects under stressful cues by measuring the germination efficiency, oxidative-biomarkers, enzymatic and non-enzymatic antioxidants, proline and nitrogen metabolism, stomatal dynamics, and ABA content. Taken together, the study shows a promising approach in salt tolerance and suggests that mechanisms of inducing the salt tolerance depend on proline metabolism, ions accumulation, and defense mechanisms. This study ascertains the queries regarding the correlation between nanoparticles use and traditional agriculture methodology; also significantly facilitates to reach the goal of sustainable developments for increasing crop productivity via much safer and greener approachability.
Iram Wahid; Sarika Kumari; Rafiq Ahmad; Sofi Hussain; Saud Alamri; Manzer Siddiqui; M. Khan. Silver Nanoparticle Regulates Salt Tolerance in Wheat Through Changes in ABA Concentration, Ion Homeostasis, and DefenseSystems. Biomolecules 2020, 10, 1506 .
AMA StyleIram Wahid, Sarika Kumari, Rafiq Ahmad, Sofi Hussain, Saud Alamri, Manzer Siddiqui, M. Khan. Silver Nanoparticle Regulates Salt Tolerance in Wheat Through Changes in ABA Concentration, Ion Homeostasis, and DefenseSystems. Biomolecules. 2020; 10 (11):1506.
Chicago/Turabian StyleIram Wahid; Sarika Kumari; Rafiq Ahmad; Sofi Hussain; Saud Alamri; Manzer Siddiqui; M. Khan. 2020. "Silver Nanoparticle Regulates Salt Tolerance in Wheat Through Changes in ABA Concentration, Ion Homeostasis, and DefenseSystems." Biomolecules 10, no. 11: 1506.
The present study evaluated the performance of microalgae Chlorella vulgaris in an Enterobacter sp. MN17-assisted textile industry wastewater treatment system for decolorization, removal of heavy metals (Cu, Cr, Pb, and Cd), and chemical oxygen demand (COD). Different dilutions (5, 10, and 20%) of wastewater were prepared to decrease the pollutant toxicity for culturing microalgae and bacteria. Reduction of color, COD, and metal contents by microalgal treatment of wastewater varied greatly, while removal efficiency (RE) was significantly enhanced when endophytic bacterial strain MN17 inoculum was applied. Most notable, results were found at a 5% dilution level by Enterobacter sp. MN17-inoculated C. vulgaris medium, as chromium (Cr), cadmium (Cd), copper (Cu), and lead (Pb) concentrations were decreased from 1.32 to 0.27 mg L−1 (79% decrease), 0.79–0.14 mg L−1 (93% decrease), 1.33–0.36 mg L−1 (72% decrease), and 1.2–0.25 mg L−1 (79% decrease), respectively. The values of COD and color were also significantly decreased by 74% and 70%, respectively, by a C. vulgaris–Enterobacter sp. MN17 consortium. The present investigation revealed that bacterial inoculation of microalgae significantly enhanced the removal of coloring agents and heavy metals from textile wastewater by stimulating the growth of algal biomass. This study manifested the usefulness of microalgae–bacterial mutualism for the remediation of heavy metals, COD, and color in industrial effluents. Microalgae consortia with growth promoting bacteria could be a breakthrough for better bioremediation and bioprocess economy. Thus, further studies are needed for successful integration of microalgae–plant growth promoting bacterial (PGPB) consortium for wastewater treatments.
Muhammad Mubashar; Muhammad Naveed; Adnan Mustafa; Sobia Ashraf; Khurram Shehzad Baig; Saud Alamri; Manzer Siddiqui; Magdalena Zabochnicka-Świątek; Michał Szota; Hazem Kalaji. Experimental Investigation of Chlorella vulgaris and Enterobacter sp. MN17 for Decolorization and Removal of Heavy Metals from Textile Wastewater. Water 2020, 12, 3034 .
AMA StyleMuhammad Mubashar, Muhammad Naveed, Adnan Mustafa, Sobia Ashraf, Khurram Shehzad Baig, Saud Alamri, Manzer Siddiqui, Magdalena Zabochnicka-Świątek, Michał Szota, Hazem Kalaji. Experimental Investigation of Chlorella vulgaris and Enterobacter sp. MN17 for Decolorization and Removal of Heavy Metals from Textile Wastewater. Water. 2020; 12 (11):3034.
Chicago/Turabian StyleMuhammad Mubashar; Muhammad Naveed; Adnan Mustafa; Sobia Ashraf; Khurram Shehzad Baig; Saud Alamri; Manzer Siddiqui; Magdalena Zabochnicka-Świątek; Michał Szota; Hazem Kalaji. 2020. "Experimental Investigation of Chlorella vulgaris and Enterobacter sp. MN17 for Decolorization and Removal of Heavy Metals from Textile Wastewater." Water 12, no. 11: 3034.
A research study was established at the research farm of the University of Agriculture, Peshawar during winter 2018–2019. Commercial biochars were given to the experimental site from 2014 to summer 2018 and received 0.95, 130 and 60 tons ha−1 of biochar by various treatments viz., (Biochar1) BC1, (Biochar2) BC2, (Biochar3) BC3 and (Biochar4) BC4, respectively. This piece of work was conducted within the same study to find the long-term influence of bi0char on the fertility of the soil, fixation of N2, as well as the yie1d of chickpea under a mung–chickpea cropping system. A split pl0t arrangement was carried out by RCBD (Randomized Complete Block Design) to evaluate the study. Twenty-five kilograms of N ha−1 were given as a starter dosage to every plot. Phosphorous and potassium were applied at two levels (half (45:30 kg ha−1) and full (90:60 kg ha−1) recommended doses) to each of the four biochar treatments. The chickpea crop parameters measured were the numbers and masses of the nodules, N2 fixation and grain yield. Soil parameters recorded were Soil Organic Matter (SOM), total N and mineral N. The aforementioned soil parameters were recorded after harvesting. The results showed that nodulation in chickpea, grain yield and nutrient uptake were significantly enhanced by phosphorous and potassium mineral fertilizers. The application of biochar 95 tons ha−1 significantly enhanced number of nodules i-e (122), however statistically similar response in terms of nodules number was also noted with treatment of 130 tons ha−1. The results further revealed a significant difference in terms of organic matter (OM) (%) between the half and full mineral fertilizer treatments. With the application of 130 tons ha−1 of biochar, the OM enhanced from 1.67% in the control treatment, to 2.59%. However, total and mineral nitrogen were not statistically enhanced by the mineral fertilizer treatment. With regard to biochar treatments, total and mineral N enhanced when compared with the control treatment. The highest total N of 0.082% and mineral nitrogen of 73 mg kg−1 in the soil were recorded at 130 tons ha−1 of biochar, while the lowest total N (0.049%) and mineral nitrogen (54 mg kg−1) in the soil were recorded in the control treatment. The collaborative influence of mineral fertilizers and biochars was found to be generally non-significant for most of the soil and plant parameters. It could be concluded that the aforementioned parameters were greater for treatments receiving biochar at 95 tons or more per hectare over the last several years, and that the combination of lower doses of mineral fertilizers further improved the performance of biochar.
Shadman Khan; Zahir Shah; Ishaq Mian; Khadim Dawar; Muhammad Tariq; Bushra Khan; Maria Mussarat; Hazrat Amin; Muhammad Ismail; Shamsher Ali; Tasneem Shah; Saud Alamri; Manzer Siddiqui; Muhammad Adnan; Muhammad Romman; Shah Fahad; Aqib Noman; Abid Kamal. Soil Fertility, N2 Fixation and Yield of Chickpea as Influenced by Long-Term Biochar Application Under Mung–Chickpea Cropping System. Sustainability 2020, 12, 9008 .
AMA StyleShadman Khan, Zahir Shah, Ishaq Mian, Khadim Dawar, Muhammad Tariq, Bushra Khan, Maria Mussarat, Hazrat Amin, Muhammad Ismail, Shamsher Ali, Tasneem Shah, Saud Alamri, Manzer Siddiqui, Muhammad Adnan, Muhammad Romman, Shah Fahad, Aqib Noman, Abid Kamal. Soil Fertility, N2 Fixation and Yield of Chickpea as Influenced by Long-Term Biochar Application Under Mung–Chickpea Cropping System. Sustainability. 2020; 12 (21):9008.
Chicago/Turabian StyleShadman Khan; Zahir Shah; Ishaq Mian; Khadim Dawar; Muhammad Tariq; Bushra Khan; Maria Mussarat; Hazrat Amin; Muhammad Ismail; Shamsher Ali; Tasneem Shah; Saud Alamri; Manzer Siddiqui; Muhammad Adnan; Muhammad Romman; Shah Fahad; Aqib Noman; Abid Kamal. 2020. "Soil Fertility, N2 Fixation and Yield of Chickpea as Influenced by Long-Term Biochar Application Under Mung–Chickpea Cropping System." Sustainability 12, no. 21: 9008.