This page has only limited features, please log in for full access.
Although viruses infect various organs and are associated with diseases, there may be many unidentified pathogenic viruses. The recent development of next-generation sequencing technologies has facilitated the establishment of an environmental viral metagenomic approach targeting the intracellular viral genome. However, an efficient method for the detection of a viral genome derived from an RNA virus in animal or human samples has not been established. Here, we established a method for the efficient detection of RNA viruses in human clinical samples. We then tested the efficiency of the method compared to other conventional methods by using tissue samples collected from 57 recipients of living donor liver transplantations performed between June 2017 and February 2019 at Kyushu University Hospital. The viral read ratio in human clinical samples was higher by the new method than by the other conventional methods. In addition, the new method correctly identified viral RNA from liver tissues infected with hepatitis C virus. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases.
Takuma Izumi; Yuhei Morioka; Syun-Ichi Urayama; Daisuke Motooka; Tomokazu Tamura; Takahiro Kawagishi; Yuta Kanai; Takeshi Kobayashi; Chikako Ono; Akinari Morinaga; Takahiro Tomiyama; Norifumi Iseda; Yukiko Kosai; Shoichi Inokuchi; Shota Nakamura; Tomohisa Tanaka; Kohji Moriishi; Hiroaki Kariwa; Tomoharu Yoshizumi; Masaki Mori; Yoshiharu Matsuura; Takasuke Fukuhara. DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples. Viruses 2021, 13, 1310 .
AMA StyleTakuma Izumi, Yuhei Morioka, Syun-Ichi Urayama, Daisuke Motooka, Tomokazu Tamura, Takahiro Kawagishi, Yuta Kanai, Takeshi Kobayashi, Chikako Ono, Akinari Morinaga, Takahiro Tomiyama, Norifumi Iseda, Yukiko Kosai, Shoichi Inokuchi, Shota Nakamura, Tomohisa Tanaka, Kohji Moriishi, Hiroaki Kariwa, Tomoharu Yoshizumi, Masaki Mori, Yoshiharu Matsuura, Takasuke Fukuhara. DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples. Viruses. 2021; 13 (7):1310.
Chicago/Turabian StyleTakuma Izumi; Yuhei Morioka; Syun-Ichi Urayama; Daisuke Motooka; Tomokazu Tamura; Takahiro Kawagishi; Yuta Kanai; Takeshi Kobayashi; Chikako Ono; Akinari Morinaga; Takahiro Tomiyama; Norifumi Iseda; Yukiko Kosai; Shoichi Inokuchi; Shota Nakamura; Tomohisa Tanaka; Kohji Moriishi; Hiroaki Kariwa; Tomoharu Yoshizumi; Masaki Mori; Yoshiharu Matsuura; Takasuke Fukuhara. 2021. "DsRNA Sequencing for RNA Virus Surveillance Using Human Clinical Samples." Viruses 13, no. 7: 1310.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development.
Mai Kishimoto; Kentaro Uemura; Takao Sanaki; Akihiko Sato; William Hall; Hiroaki Kariwa; Yasuko Orba; Hirofumi Sawa; Michihito Sasaki. TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses 2021, 13, 384 .
AMA StyleMai Kishimoto, Kentaro Uemura, Takao Sanaki, Akihiko Sato, William Hall, Hiroaki Kariwa, Yasuko Orba, Hirofumi Sawa, Michihito Sasaki. TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses. 2021; 13 (3):384.
Chicago/Turabian StyleMai Kishimoto; Kentaro Uemura; Takao Sanaki; Akihiko Sato; William Hall; Hiroaki Kariwa; Yasuko Orba; Hirofumi Sawa; Michihito Sasaki. 2021. "TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein." Viruses 13, no. 3: 384.
Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. IgM antibody detection is useful for the serological diagnosis of TBEV infection, because IgM has high specificity for each flavivirus and indicates a recent infection. Commercial IgM-ELISA kits are somewhat expensive and difficulties in their sensitivity have been suggested due to their format and formalin-inactivated antigens. Therefore, the development of an inexpensive IgM-ELISA with high specificity and sensitivity is needed. In this study, a μ-capture ELISA was developed to detect TBEV-specific IgM antibodies using subviral particles (SPs) with strep-tag (strep-SP-IgM-ELISA). The results of our strep-SP-IgM-ELISA were highly correlated with diagnoses made by the neutralization test (sensitivity: 94.1%), and our strep-SP-IgM-ELISA could detect anti-TBEV IgM antibodies in patients who could not be diagnosed with the neutralization test. Besides, 51 of 52 positive samples by a commercial IgM-ELISA were also diagnosed as positive by our strep-SP-IgM-ELISA (98.1%), and our strep-SP-IgM-ELISA could detect anti-TBEV IgM antibodies in all samples that were inconclusive based on the commercial IgM-ELISA. Our strep-SP-IgM-ELISA will be useful for diagnoses in TBE-endemic areas.
Miki Nakayasu; Minato Hirano; Memi Muto; Shintaro Kobayashi; Hiroaki Kariwa; Kentaro Yoshii. Development of a serodiagnostic IgM-ELISA for tick-borne encephalitis virus using subviral particles with strep-tag. Ticks and Tick-borne Diseases 2018, 9, 1391 -1394.
AMA StyleMiki Nakayasu, Minato Hirano, Memi Muto, Shintaro Kobayashi, Hiroaki Kariwa, Kentaro Yoshii. Development of a serodiagnostic IgM-ELISA for tick-borne encephalitis virus using subviral particles with strep-tag. Ticks and Tick-borne Diseases. 2018; 9 (6):1391-1394.
Chicago/Turabian StyleMiki Nakayasu; Minato Hirano; Memi Muto; Shintaro Kobayashi; Hiroaki Kariwa; Kentaro Yoshii. 2018. "Development of a serodiagnostic IgM-ELISA for tick-borne encephalitis virus using subviral particles with strep-tag." Ticks and Tick-borne Diseases 9, no. 6: 1391-1394.
Tick-borne encephalitis virus (TBEV) is maintained between ticks and mammals in nature and causes severe neurological disease in human. However, the mechanism of viral pathogenicity is unknown. Previously, we showed that the deletion in the variable region of the 3'-untranslated region (UTR) is involved in the pathogenicity of the strains from the Far-Eastern subtype of TBEV. To investigate the detailed function of the variable region, we constructed recombinant TBEV with partial deletions in the region. In a mouse model, the partial deletions drastically increased the virulence of the virus, with no effect on virus multiplication in mouse brain. Furthermore, the mutations did not affect the production of subgenomic flavivirus RNA from the 3'-UTR, and the induction of interferon (IFN) and IFN-stimulated genes. These data suggested that the conformational structure of the variable region is associated with the pathogenicity of the Far-Eastern subtype of TBEV. These findings provide a foundation for further research to identify the pathogenic mechanisms of TBEV.
Mizuki Sakai; Memi Muto; Minato Hirano; Hiroaki Kariwa; Kentaro Yoshii. Virulence of tick-borne encephalitis virus is associated with intact conformational viral RNA structures in the variable region of the 3′-UTR. Virus Research 2015, 203, 36 -40.
AMA StyleMizuki Sakai, Memi Muto, Minato Hirano, Hiroaki Kariwa, Kentaro Yoshii. Virulence of tick-borne encephalitis virus is associated with intact conformational viral RNA structures in the variable region of the 3′-UTR. Virus Research. 2015; 203 ():36-40.
Chicago/Turabian StyleMizuki Sakai; Memi Muto; Minato Hirano; Hiroaki Kariwa; Kentaro Yoshii. 2015. "Virulence of tick-borne encephalitis virus is associated with intact conformational viral RNA structures in the variable region of the 3′-UTR." Virus Research 203, no. : 36-40.
Nozyechi N. Chidumayo; Kentaro Yoshii; Ngonda Saasa; Misuki Sakai; Hiroaki Kariwa. Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins. Diagnostic Microbiology and Infectious Disease 2014, 78, 373 -378.
AMA StyleNozyechi N. Chidumayo, Kentaro Yoshii, Ngonda Saasa, Misuki Sakai, Hiroaki Kariwa. Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins. Diagnostic Microbiology and Infectious Disease. 2014; 78 (4):373-378.
Chicago/Turabian StyleNozyechi N. Chidumayo; Kentaro Yoshii; Ngonda Saasa; Misuki Sakai; Hiroaki Kariwa. 2014. "Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins." Diagnostic Microbiology and Infectious Disease 78, no. 4: 373-378.
In this review, we discuss the possibility that the glycosylation of West Nile (WN) virus E-protein may be associated with enhanced pathogenicity and higher replication of WN virus. The results indicate that E-protein glycosylation allows the virus to multiply in a heat-stable manner and therefore, has a critical role in enhanced viremic levels and virulence of WN virus in young-chick infection model. The effect of the glycosylation of the E protein on the pathogenicity of WN virus in young chicks was further investigated. The results indicate that glycosylation of the WN virus E protein is important for viral multiplication in peripheral organs and that it is associated with the strong pathogenicity of WN virus in birds. The micro-focus reduction neutralization test (FRNT) in which a large number of serum samples can be handled at once with a small volume (15 μL) of serum was useful for differential diagnosis between Japanese encephalitis and WN virus infections in infected chicks. Serological investigation was performed among wild birds in the Far Eastern region of Russia using the FRNT. Antibodies specific to WN virus were detected in 21 samples of resident and migratory birds out of 145 wild bird samples in the region.
Hiroaki Kariwa; Ryo Murata; Masashi Totani; Kentaro Yoshii; Ikuo Takashima. Increased Pathogenicity of West Nile Virus (WNV) by Glycosylation of Envelope Protein and Seroprevalence of WNV in Wild Birds in Far Eastern Russia. International Journal of Environmental Research and Public Health 2013, 10, 7144 -7164.
AMA StyleHiroaki Kariwa, Ryo Murata, Masashi Totani, Kentaro Yoshii, Ikuo Takashima. Increased Pathogenicity of West Nile Virus (WNV) by Glycosylation of Envelope Protein and Seroprevalence of WNV in Wild Birds in Far Eastern Russia. International Journal of Environmental Research and Public Health. 2013; 10 (12):7144-7164.
Chicago/Turabian StyleHiroaki Kariwa; Ryo Murata; Masashi Totani; Kentaro Yoshii; Ikuo Takashima. 2013. "Increased Pathogenicity of West Nile Virus (WNV) by Glycosylation of Envelope Protein and Seroprevalence of WNV in Wild Birds in Far Eastern Russia." International Journal of Environmental Research and Public Health 10, no. 12: 7144-7164.
New World hantaviruses were divided into five groups based on the amino acid sequence variability of the internal variable region (around 230–302 amino acids) of hantavirus nucleocapsid protein (NP). Sin Nombre virus (SNV), Andes virus, Black Creek Canal virus (BCCV), Carrizal virus (CARV) and Cano Delgadito virus belong to groups 1, 2, 3, 4 and 5, respectively. Patient and rodent sera were serotyped successfully by an enzyme-linked immunosorbent assay (ELISA) with recombinant truncated NP lacking 99 N-terminal amino acids (trNP100) of SNV, CARV and BCCV. The trNP100 of BCCV showed lower reactivity to heterologous sera. In contrast, whole recombinant NP antigens detected both homologous and heterologous antibodies equally. The results together with results of a previous study suggest that trNP100 can distinguish infections among viruses in groups 1, 2, 3 and 4 of New World hantaviruses. The serotyping ELISA with trNP100 is useful for epidemiological surveillance in humans and rodents.
Takaaki Koma; Kumiko Yoshimatsu; Midori Taruishi; Daisuke Miyashita; Rika Endo; Kenta Shimizu; Shumpei P. Yasuda; Takako Amada; Takahiro Seto; Ryo Murata; Haruka Yoshida; Hiroaki Kariwa; Ikuo Takashima; Jiro Arikawa. Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection. Journal of Virological Methods 2012, 185, 74 -81.
AMA StyleTakaaki Koma, Kumiko Yoshimatsu, Midori Taruishi, Daisuke Miyashita, Rika Endo, Kenta Shimizu, Shumpei P. Yasuda, Takako Amada, Takahiro Seto, Ryo Murata, Haruka Yoshida, Hiroaki Kariwa, Ikuo Takashima, Jiro Arikawa. Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection. Journal of Virological Methods. 2012; 185 (1):74-81.
Chicago/Turabian StyleTakaaki Koma; Kumiko Yoshimatsu; Midori Taruishi; Daisuke Miyashita; Rika Endo; Kenta Shimizu; Shumpei P. Yasuda; Takako Amada; Takahiro Seto; Ryo Murata; Haruka Yoshida; Hiroaki Kariwa; Ikuo Takashima; Jiro Arikawa. 2012. "Development of a serotyping enzyme-linked immunosorbent assay system based on recombinant truncated hantavirus nucleocapsid proteins for New World hantavirus infection." Journal of Virological Methods 185, no. 1: 74-81.
The hantavirus nucleocapsid (N) protein is an important immunogen that stimulates a strong and cross-reactive immune response in humans and rodents. A large proportion of the response to N protein has been found to target its N-terminus. However, the exact nature of this bias towards the N-terminus is not yet fully understood. We characterized six monoclonal antibodies (mAbs) against the N protein of Montano virus (MTNV), a Mexican hantavirus. Five of these mAbs recognized eight American hantaviruses and six European and Asian hantaviruses, but not the Soricomorpha-borne Thottapalayam hantavirus. The N protein-reactive binding regions of the five mAbs were mapped to discontinuous epitopes within the N-terminal 13-51 amino acid residues, while a single serotype-specific mAb was mapped to residues 1-25 and 49-75. Our findings suggest that discontinuous epitopes at the N-terminus are conserved, at least in rodent-borne hantaviruses, and that they contribute considerably to N protein cross-reactivity.
Ngonda Saasa; Haruka Yoshida; Kenta Shimizu; Cornelio Sánchez-Hernández; María De Lourdes Romero-Almaraz; Takaaki Koma; Takahiro Sanada; Takahiro Seto; Kentaro Yoshii; Celso Ramos; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima; Hiroaki Kariwa. The N-terminus of the Montano virus nucleocapsid protein possesses broadly cross-reactive conformation-dependent epitopes conserved in rodent-borne hantaviruses. Virology 2012, 428, 48 -57.
AMA StyleNgonda Saasa, Haruka Yoshida, Kenta Shimizu, Cornelio Sánchez-Hernández, María De Lourdes Romero-Almaraz, Takaaki Koma, Takahiro Sanada, Takahiro Seto, Kentaro Yoshii, Celso Ramos, Kumiko Yoshimatsu, Jiro Arikawa, Ikuo Takashima, Hiroaki Kariwa. The N-terminus of the Montano virus nucleocapsid protein possesses broadly cross-reactive conformation-dependent epitopes conserved in rodent-borne hantaviruses. Virology. 2012; 428 (1):48-57.
Chicago/Turabian StyleNgonda Saasa; Haruka Yoshida; Kenta Shimizu; Cornelio Sánchez-Hernández; María De Lourdes Romero-Almaraz; Takaaki Koma; Takahiro Sanada; Takahiro Seto; Kentaro Yoshii; Celso Ramos; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima; Hiroaki Kariwa. 2012. "The N-terminus of the Montano virus nucleocapsid protein possesses broadly cross-reactive conformation-dependent epitopes conserved in rodent-borne hantaviruses." Virology 428, no. 1: 48-57.
Hemorrhagic fever with renal syndrome (HFRS) is a serious public health issue in Far East Russia. Two different hantaviruses were isolated from rodents captured in the Khabarovsk region: Amur virus (AMRV; Khekhtsir/AP209/2005 strain from Apodemus peninsulae) and Hantaan virus (HTNV; Galkino/AA57/2002 strain from A. agrarius). Genetic analysis of the new isolates revealed that the M and L segments were apparently different between AMRV and HTNV, but S segments of the two viruses were closer. The antigenicities of AMRV, HTNV, and Seoul virus (SEOV) were differentiated by cross-neutralization. Serological differential diagnoses of 67 HFRS patients in the Prymorsky and Khabarovsk regions of Far East Russia were conducted using a neutralization test. The results revealed that the major cause of HFRS varied with location in Far East Russia: SEOV for Vladivostok city in the Prymorsky region, AMRV in rural areas of the Primorsky region, and probably HTNV for the Khabarovsk region.
Hiroaki Kariwa; Ngonda Saasa; Kumiko Yoshimatsu; Leonid Ivanov; Jiro Arikawa; Keisuke Yoshikawa; Yoichi Tanikawa; Raisa Slonova; Tatyana A. Zakharycheva; Takahiro Seto; Kentaro Yoshii; Ichiro Nakamura; Takahiro Sanada; Ikuo Takashima. Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region. The American Journal of Tropical Medicine and Hygiene 2012, 86, 545 -553.
AMA StyleHiroaki Kariwa, Ngonda Saasa, Kumiko Yoshimatsu, Leonid Ivanov, Jiro Arikawa, Keisuke Yoshikawa, Yoichi Tanikawa, Raisa Slonova, Tatyana A. Zakharycheva, Takahiro Seto, Kentaro Yoshii, Ichiro Nakamura, Takahiro Sanada, Ikuo Takashima. Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region. The American Journal of Tropical Medicine and Hygiene. 2012; 86 (3):545-553.
Chicago/Turabian StyleHiroaki Kariwa; Ngonda Saasa; Kumiko Yoshimatsu; Leonid Ivanov; Jiro Arikawa; Keisuke Yoshikawa; Yoichi Tanikawa; Raisa Slonova; Tatyana A. Zakharycheva; Takahiro Seto; Kentaro Yoshii; Ichiro Nakamura; Takahiro Sanada; Ikuo Takashima. 2012. "Isolation and Characterization of Hantaviruses in Far East Russia and Etiology of Hemorrhagic Fever with Renal Syndrome in the Region." The American Journal of Tropical Medicine and Hygiene 86, no. 3: 545-553.
Masashi Totani; Kentaro Yoshii; Hiroaki Kariwa; Ikuo Takashima. Glycosylation of the Envelope Protein of West Nile Virus Affects Its Replication in Chicks. Avian Diseases Digest 2011, 6, e5 -e6.
AMA StyleMasashi Totani, Kentaro Yoshii, Hiroaki Kariwa, Ikuo Takashima. Glycosylation of the Envelope Protein of West Nile Virus Affects Its Replication in Chicks. Avian Diseases Digest. 2011; 6 (4):e5-e6.
Chicago/Turabian StyleMasashi Totani; Kentaro Yoshii; Hiroaki Kariwa; Ikuo Takashima. 2011. "Glycosylation of the Envelope Protein of West Nile Virus Affects Its Replication in Chicks." Avian Diseases Digest 6, no. 4: e5-e6.
A variety of hantaviruses are harbored by rodents in North and South America, some of which can cause hantavirus pulmonary syndrome. To obtain greater evolutionary insight into hantaviruses in the Americas, a total of 211 rodents were captured in the Mexican states of Guerrero and Morelos in 2006. Anti-hantavirus antibodies were detected in 27 of 211 serum samples (12.8%) by ELISA. The distribution of seropositive rodents was: 17 Peromyscus beatae, 1 Megadontomys thomasi, 1 Neotoma picta, 6 Reithrodontomys sumichrasti, and 2 Reithrodontomys megalotis. The hantavirus small (S), medium (M), and large (L) genome segments from P. beatae, R. sumichrasti, and R. megalotis were amplified and the sequences covering the open reading frames were determined. The hantaviruses from P. beatae, R. sumichrasti, and R. megalotis were provisionally designated Montano (MTN), Carrizal (CAR), and Huitzilac (HUI), respectively. The M segment amino acid identities among the Mexican hantaviruses were 80.8–93.0%. When these M segments were compared to those of known hantaviruses, MTN virus was most closely related to Limestone Canyon (LSC) virus (88.9% amino acid identity), while the CAR and HUI viruses were most closely related to El Moro Canyon (ELMC) virus (90–91% identity). Phylogenetic analysis revealed that the MTN, CAR, and HUI viruses occupy a monophyletic clade with the LSC, ELMC, and Rio Segundo viruses, which are harbored by Peromyscus boylii, R. megalotis, and Reithrodontomys mexicanus, respectively. The data obtained in this study provide important information for understanding the evolution of hantaviruses in the Americas.
Hiroaki Kariwa; Haruka Yoshida; Cornelio Sánchez-Hernández; María De Lourdes Romero-Almaraz; José Alberto Almazán-Catalán; Celso Ramos; Daisuke Miyashita; Takahiro Seto; Ayako Takano; Masashi Totani; Ryo Murata; Ngonda Saasa; Mariko Ishizuka; Takahiro Sanada; Kentaro Yoshii; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima. Genetic diversity of hantaviruses in Mexico: Identification of three novel hantaviruses from Neotominae rodents. Virus Research 2011, 163, 486 -494.
AMA StyleHiroaki Kariwa, Haruka Yoshida, Cornelio Sánchez-Hernández, María De Lourdes Romero-Almaraz, José Alberto Almazán-Catalán, Celso Ramos, Daisuke Miyashita, Takahiro Seto, Ayako Takano, Masashi Totani, Ryo Murata, Ngonda Saasa, Mariko Ishizuka, Takahiro Sanada, Kentaro Yoshii, Kumiko Yoshimatsu, Jiro Arikawa, Ikuo Takashima. Genetic diversity of hantaviruses in Mexico: Identification of three novel hantaviruses from Neotominae rodents. Virus Research. 2011; 163 (2):486-494.
Chicago/Turabian StyleHiroaki Kariwa; Haruka Yoshida; Cornelio Sánchez-Hernández; María De Lourdes Romero-Almaraz; José Alberto Almazán-Catalán; Celso Ramos; Daisuke Miyashita; Takahiro Seto; Ayako Takano; Masashi Totani; Ryo Murata; Ngonda Saasa; Mariko Ishizuka; Takahiro Sanada; Kentaro Yoshii; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima. 2011. "Genetic diversity of hantaviruses in Mexico: Identification of three novel hantaviruses from Neotominae rodents." Virus Research 163, no. 2: 486-494.
The mechanism of hantavirus persistent infection in natural hosts is poorly understood due to a lack of laboratory animal models. Herein, we report that Syrian hamsters (Mesocricetus auratus) infected with Puumala virus (PUUV) at 4 weeks old show persistent infection without clinical symptoms for more than 2 months. IgG and IgM antibodies against the viral nucleocapsid protein and neutralizing antibody were first detectable at 14 days postinoculation (dpi) and maintained through 70 dpi. Viral RNA was first detected from 3 dpi in lungs and blood clots, and was detected in all tissues tested at 7 dpi. The viral RNA persisted for at least 70 days in the lungs, kidney, spleen, heart, and brain. The highest level of RNA copies was observed at 14 dpi in the lungs. Slight inflammatory reactions were observed in the lungs, adrenal glands, and brain. Immunohistochemical analysis revealed that PUUV antigen persisted until 56 dpi in the kidneys and adrenal glands. Infected hamsters showed no body weight loss or clinical signs. These results indicate that PUUV infection in hamsters is quite similar to the hantavirus infection of natural host rodents.
Takahiro Sanada; Hiroaki Kariwa; Noriyo Nagata; Yoichi Tanikawa; Takahiro Seto; Kumiko Yoshimatsu; Jiro Arikawa; Kentaro Yoshii; Ikuo Takashima. Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts. Virus Research 2011, 160, 108 -119.
AMA StyleTakahiro Sanada, Hiroaki Kariwa, Noriyo Nagata, Yoichi Tanikawa, Takahiro Seto, Kumiko Yoshimatsu, Jiro Arikawa, Kentaro Yoshii, Ikuo Takashima. Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts. Virus Research. 2011; 160 (1-2):108-119.
Chicago/Turabian StyleTakahiro Sanada; Hiroaki Kariwa; Noriyo Nagata; Yoichi Tanikawa; Takahiro Seto; Kumiko Yoshimatsu; Jiro Arikawa; Kentaro Yoshii; Ikuo Takashima. 2011. "Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts." Virus Research 160, no. 1-2: 108-119.
Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis serocomplex of flaviviruses, and causes hemorrhagic disease in humans. In this study, an infectious cDNA of OHFV was constructed to investigate the molecular mechanisms involved in OHFV pathogenesis for the first time. Our cDNA clone was capable of producing infectious virus which is genetically identical to the parental Guriev strain, and the recombinant virus showed similar biological properties to the parental virus including growth kinetics and virulence characteristics. While characterizing the cDNAs, fortuitous mutations at NS2A position 46 and NS5 position 836 were found to affect viral production. By using a viral replicon expressing luciferase, it was shown that both of the mutations produced a defect in RNA replication and that the NS5 mutation induced a temperature-sensitive phenotype, indicating the importance of these residues in RNA replication. This infectious cDNA will be a useful tool to study the replication and pathogenesis of OHFV
Kentaro Yoshii; Manabu Igarashi; Kimihito Ito; Hiroaki Kariwa; Michael R. Holbrook; Ikuo Takashima. Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5. Virus Research 2011, 155, 61 -68.
AMA StyleKentaro Yoshii, Manabu Igarashi, Kimihito Ito, Hiroaki Kariwa, Michael R. Holbrook, Ikuo Takashima. Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5. Virus Research. 2011; 155 (1):61-68.
Chicago/Turabian StyleKentaro Yoshii; Manabu Igarashi; Kimihito Ito; Hiroaki Kariwa; Michael R. Holbrook; Ikuo Takashima. 2011. "Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5." Virus Research 155, no. 1: 61-68.
Tick-borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. An epizootiological survey of wild rodents is effective to detect TBE virus-endemic areas; however, limited serological diagnostic methods are available to detect anti-TBE virus antibodies in wild rodents. In this study, ELISAs for the detection of rodent antibodies against the TBE virus were developed using two recombinant proteins, domain III of the E protein (EdIII) and subviral particles (SPs), as the antigens. As compared with the neutralization test, the ELISA using EdIII had 77.1% sensitivity and 80.0% specificity, and the ELISA using SPs had 91.4% sensitivity and 100% specificity. Furthermore, when the ELISAs were applied to the epizootiological survey in the TBE virus-endemic area, both of the ELISAs was able to detect wild rodents with TBE virus-specific antibodies. This is the first study to show that ELISAs using recombinant antigens can be safe and useful in the detection of TBE virus-infected wild rodents in epizootiological research.
Ayae Ikawa‐Yoshida; Kentaro Yoshii; Kazue Kuwahara; Mayumi Obara; Hiroaki Kariwa; Ikuo Takashima. Development of an ELISA system for tick-borne encephalitis virus infection in rodents. Microbiology and Immunology 2011, 55, 100 -107.
AMA StyleAyae Ikawa‐Yoshida, Kentaro Yoshii, Kazue Kuwahara, Mayumi Obara, Hiroaki Kariwa, Ikuo Takashima. Development of an ELISA system for tick-borne encephalitis virus infection in rodents. Microbiology and Immunology. 2011; 55 (2):100-107.
Chicago/Turabian StyleAyae Ikawa‐Yoshida; Kentaro Yoshii; Kazue Kuwahara; Mayumi Obara; Hiroaki Kariwa; Ikuo Takashima. 2011. "Development of an ELISA system for tick-borne encephalitis virus infection in rodents." Microbiology and Immunology 55, no. 2: 100-107.
Previously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing antibodies against TBEV as an alternative to performing neutralization tests with live virus. As markers of VLP infection, the genes for GFP and luciferase were inserted into the TBEV replicon, which was then packaged into VLPs. The reporter genes were expressed in cells that were infected with the VLPs, and this infection was inhibited by neutralizing antibodies to TBEV. Serum samples from wild rodents were used to evaluate the neutralization test using VLPs. All the sera that were positive in the conventional neutralization test were also found to be positive in the neutralization test using VLPs, and there were highly significant correlations between the neutralization titres obtained using the native virus and those using VLPs. These results indicate that VLPs that express reporter genes represent a useful and safe alternative to conventional neutralization testing using live virus.
Kentaro Yoshii; Ayae Ikawa; Yumiko Chiba; Yuki Omori; Junko Maeda; Ryo Murata; Hiroaki Kariwa; Ikuo Takashima. Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus. Journal of Virological Methods 2009, 161, 173 -176.
AMA StyleKentaro Yoshii, Ayae Ikawa, Yumiko Chiba, Yuki Omori, Junko Maeda, Ryo Murata, Hiroaki Kariwa, Ikuo Takashima. Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus. Journal of Virological Methods. 2009; 161 (1):173-176.
Chicago/Turabian StyleKentaro Yoshii; Ayae Ikawa; Yumiko Chiba; Yuki Omori; Junko Maeda; Ryo Murata; Hiroaki Kariwa; Ikuo Takashima. 2009. "Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus." Journal of Virological Methods 161, no. 1: 173-176.
Kanako Moritoh; Hideto Yamauchi; Atsushi Asano; Kentaro Yoshii; Hiroaki Kariwa; Ikuo Takashima; Norikazu Isoda; Yoshihiro Sakoda; Hiroshi Kida; Nobuya Sasaki; Takashi Agui. Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J. The Japanese journal of veterinary research 2009, 57, 1 .
AMA StyleKanako Moritoh, Hideto Yamauchi, Atsushi Asano, Kentaro Yoshii, Hiroaki Kariwa, Ikuo Takashima, Norikazu Isoda, Yoshihiro Sakoda, Hiroshi Kida, Nobuya Sasaki, Takashi Agui. Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J. The Japanese journal of veterinary research. 2009; 57 (2):1.
Chicago/Turabian StyleKanako Moritoh; Hideto Yamauchi; Atsushi Asano; Kentaro Yoshii; Hiroaki Kariwa; Ikuo Takashima; Norikazu Isoda; Yoshihiro Sakoda; Hiroshi Kida; Nobuya Sasaki; Takashi Agui. 2009. "Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J." The Japanese journal of veterinary research 57, no. 2: 1.
SARS‐CoV has four major structural proteins: the N, S, M, and E proteins. To investigate the mechanism of SARS‐CoV assembly, we cloned the genes encoding these four proteins into the eukaryotic expression vector pCAGGS and transfected them into 293T cells. When all four expression vectors were co‐transfected VLP formed, as confirmed using electron microscopy. Using a rabbit polyclonal antibody specific to the N protein, N‐protein‐containing particles similar in size to the VLP were also observed by immunoelectron microscopy, indicating that the VLP contained the N protein. Co‐immunoprecipitation analyses demonstrated an interaction between the N and M proteins, suggesting that N protein binds directly to M protein to be incorporated into VLP.
Mina Nakauchi; Hiroaki Kariwa; Yasuhiro Kon; Kentaro Yoshii; Akihiko Maeda; Ikuo Takashima. Analysis of severe acute respiratory syndrome coronavirus structural proteins in virus-like particle assembly. Microbiology and Immunology 2008, 52, 625 -630.
AMA StyleMina Nakauchi, Hiroaki Kariwa, Yasuhiro Kon, Kentaro Yoshii, Akihiko Maeda, Ikuo Takashima. Analysis of severe acute respiratory syndrome coronavirus structural proteins in virus-like particle assembly. Microbiology and Immunology. 2008; 52 (12):625-630.
Chicago/Turabian StyleMina Nakauchi; Hiroaki Kariwa; Yasuhiro Kon; Kentaro Yoshii; Akihiko Maeda; Ikuo Takashima. 2008. "Analysis of severe acute respiratory syndrome coronavirus structural proteins in virus-like particle assembly." Microbiology and Immunology 52, no. 12: 625-630.
Nur Hardy ABU Daud; Hiroaki Kariwa; Evgeniy Tkachenko; Tamara Dzagurnova; Olga Medvedkina; Petr Tkachenko; Mariko Ishizuka; Takahiro Seto; Daisuke Miyashita; Takahiro Sanada; Mina Nakauchi; Kentaro Yoshii; Akihiko Maeda; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima. Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain. The Japanese journal of veterinary research 2008, 56, 1 .
AMA StyleNur Hardy ABU Daud, Hiroaki Kariwa, Evgeniy Tkachenko, Tamara Dzagurnova, Olga Medvedkina, Petr Tkachenko, Mariko Ishizuka, Takahiro Seto, Daisuke Miyashita, Takahiro Sanada, Mina Nakauchi, Kentaro Yoshii, Akihiko Maeda, Kumiko Yoshimatsu, Jiro Arikawa, Ikuo Takashima. Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain. The Japanese journal of veterinary research. 2008; 56 (3):1.
Chicago/Turabian StyleNur Hardy ABU Daud; Hiroaki Kariwa; Evgeniy Tkachenko; Tamara Dzagurnova; Olga Medvedkina; Petr Tkachenko; Mariko Ishizuka; Takahiro Seto; Daisuke Miyashita; Takahiro Sanada; Mina Nakauchi; Kentaro Yoshii; Akihiko Maeda; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima. 2008. "Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain." The Japanese journal of veterinary research 56, no. 3: 1.
Hyun-Kyoung Lee; Byoung-Hee Lee; Noton Kumar Dutta; Seung Hyeok Seok; Min-Won Baek; Hui-Young Lee; Ng-Jae Kim; Yi-Rang Na; Kyoung-Jin Noh; Sung-Hoon Park; Hiroaki Kariwa; Mina Nakauchi; Le Quynh Mai; Suk-Jin Heo; Jae-Hak Park. Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA. Journal of Microbiology and Biotechnology 2008, 18, 1 .
AMA StyleHyun-Kyoung Lee, Byoung-Hee Lee, Noton Kumar Dutta, Seung Hyeok Seok, Min-Won Baek, Hui-Young Lee, Ng-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-Hoon Park, Hiroaki Kariwa, Mina Nakauchi, Le Quynh Mai, Suk-Jin Heo, Jae-Hak Park. Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA. Journal of Microbiology and Biotechnology. 2008; 18 (10):1.
Chicago/Turabian StyleHyun-Kyoung Lee; Byoung-Hee Lee; Noton Kumar Dutta; Seung Hyeok Seok; Min-Won Baek; Hui-Young Lee; Ng-Jae Kim; Yi-Rang Na; Kyoung-Jin Noh; Sung-Hoon Park; Hiroaki Kariwa; Mina Nakauchi; Le Quynh Mai; Suk-Jin Heo; Jae-Hak Park. 2008. "Detection of antibodies against SARS-Coronavirus using recombinant truncated nucleocapsid proteins by ELISA." Journal of Microbiology and Biotechnology 18, no. 10: 1.
Hokkaido virus (HOKV) is a member of the genus Hantavirus, in the family Bunyaviridae. To investigate HOKV infection in the host Myodes rufocanus, the grey red‐backed vole, 199 animals were captured at Tobetsu (October 2004 and July 2005) and Nakagawa (October 2004) in Hokkaido, Japan, for detection of antibody, antigen, and viral RNA. In the surveys in Tobetsu (2004) and Nakagawa (2004), seropositive animals were detected at a frequency of 6.0% (5/84) and 10.4% (5/48), respectively. No seropositive animals were detected in Tobetsu in 2005. Seroprevalence in males in Tobetsu and Nakagawa in 2004 was 25% (1/4) and 45.5% (5/11), respectively, which was higher than in females, at 5.0% (4/80) and 0% (0/37), respectively (P<0.01). These results suggest that male animals play an important role in the maintenance of HOKV in M. rufocanus. Two females were seronegative but viral RNA‐positive, indicating that these animals had acute infections before antibody was produced. Another five infected animals in Nakagawa were all male and had high levels of antibodies and viral RNA, suggesting that they had persistent infections. Viral RNA copies in organs of infected animals in Nakagawa were quantified by real‐time polymerase chain reaction. Two acutely infected animals had ≥10 times the number of RNA copies in their lungs compared to those of persistently infected animals. In most cases, lungs or spleen had the highest RNA copy number, regardless of infection status.
Nur Hardy ABU Daud; Hiroaki Kariwa; Yoich Tanikawa; Ichiro Nakamura; Takahiro Seto; Daisuke Miyashita; Kentaro Yoshii; Mina Nakauchi; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima. Mode of Infection of Hokkaido Virus (GenusHantavirus) among Grey Red-Backed Voles,Myodes rufocanus, in Hokkaido, Japan. Microbiology and Immunology 2007, 51, 1081 -1090.
AMA StyleNur Hardy ABU Daud, Hiroaki Kariwa, Yoich Tanikawa, Ichiro Nakamura, Takahiro Seto, Daisuke Miyashita, Kentaro Yoshii, Mina Nakauchi, Kumiko Yoshimatsu, Jiro Arikawa, Ikuo Takashima. Mode of Infection of Hokkaido Virus (GenusHantavirus) among Grey Red-Backed Voles,Myodes rufocanus, in Hokkaido, Japan. Microbiology and Immunology. 2007; 51 (11):1081-1090.
Chicago/Turabian StyleNur Hardy ABU Daud; Hiroaki Kariwa; Yoich Tanikawa; Ichiro Nakamura; Takahiro Seto; Daisuke Miyashita; Kentaro Yoshii; Mina Nakauchi; Kumiko Yoshimatsu; Jiro Arikawa; Ikuo Takashima. 2007. "Mode of Infection of Hokkaido Virus (GenusHantavirus) among Grey Red-Backed Voles,Myodes rufocanus, in Hokkaido, Japan." Microbiology and Immunology 51, no. 11: 1081-1090.