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Dr. Olaf Werner
Universidad de Murcia (Spain)

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0 Bioinformatics
0 Genome Analysis
0 molecular systematics
0 Molecular ecology and Evolution
0 plant genetic resources

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Preprint content
Published: 12 April 2021
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Cistus heterophyllus subsp. carthaginensis is a critically endangered plant taxon from E Spain. Only two populations, one in La Pobla de Vallbona, Valencian Community (consisting of only one plant) and the other in Llano de Beal, Cartagena municipality, Autonomous Community of the Region of Murcia, are presently known. The low number of individuals and introgression by the closely related C. albidus are the major threats to the conservation of Cistus heterophyllus subsp. carthaginensis. In the years 2007, 2008, 2011, and 2013 seeds were collected from the Murcia population within the efforts to protect this taxon. In this work, we compare the natural population of Murcia with the ex situ plants originated from the recollected seeds, the only known sample from Valencia, and a specimen of North Africa of C. heterophyllus using genotyping by sequencing. The natural population of Murcia shows clear signals of introgression by C. albidus while the ex situ plants are much less affected, suggesting that the major part of the introgression of the natural populations took place after the specimens for the ex situ collection were taken. The Murcian samples seem not to be very close to the Valencian plant, but the systematic relationships among the studied populations remain unclear. The ex situ conservation efforts are a key to the conservation of this taxon.

ACS Style

Olaf Werner; María José Vicente; Mayra Aguado; Jesús Robles; Rosa María Ros. Ex situ conservation of Cistus heterophyllus subsp. carthaginensis (Cistaceae) saves the taxon from introgression by Cistus albidus. 2021, 1 .

AMA Style

Olaf Werner, María José Vicente, Mayra Aguado, Jesús Robles, Rosa María Ros. Ex situ conservation of Cistus heterophyllus subsp. carthaginensis (Cistaceae) saves the taxon from introgression by Cistus albidus. . 2021; ():1.

Chicago/Turabian Style

Olaf Werner; María José Vicente; Mayra Aguado; Jesús Robles; Rosa María Ros. 2021. "Ex situ conservation of Cistus heterophyllus subsp. carthaginensis (Cistaceae) saves the taxon from introgression by Cistus albidus." , no. : 1.

Journal article
Published: 02 February 2021 in Journal of Fungi
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Most of the rock-inhabiting fungi are meristematic and melanized microorganisms often associated with monument biodeterioration. In previous microbial profiling of the Egyptian Djoser pyramid, a Pseudotaeniolina globosa isolate was found. The current study aimed to characterize the P. globosa isolated from the Djoser pyramid compared with an Italian isolate at morphological, physiological, and molecular levels. Experiments were carried out to test temperature, salinity, and pH preferences, as well as stress tolerance to UV radiation and high temperature, in addition to a multi-locus genotyping using ITS, nrSSU or 18S, nrLSU or 28S, BT2, and RPB2 markers. Morphological and molecular data confirmed the con-specificity of the two isolates. However, the Egyptian isolate showed a wider range of growth at different environmental conditions being much more tolerant to a wider range of temperature (4–37 °C) and pH values (3.0–9.0 pH) than the Italian (10–30 °C, 4.0–6.0 pH), and more tolerant to extreme salinity levels (5 M NaCl), compared to the lowest in the Italian isolate (0.2 M NaCl). Besides, the Egyptian isolate was more tolerant to high temperature than the Italian isolate since it was able to survive after exposure to up to 85 °C for 5 min, and was not affected for up to 9 h of UV exposure, while the Italian one could not regrow after the same treatments. The Pseudotaeniolina globosa species was attributed to the family Teratosphaeriaceae of the order Capnodiales, class Dothideomycetes. Our results demonstrated that the Egyptian isolate could be considered an ecotype well adapted to harsh and extreme environments. Its potential bio-deteriorating effect on such an important cultural heritage requires special attention to design and conservation plans and solutions to limit its presence and extension in the studied pyramid and surrounding archaeological sites.

ACS Style

Samah Mohamed Rizk; Mahmoud Magdy; Filomena De Leo; Olaf Werner; Mohamed Abdel-Salam Rashed; Rosa Maria Ros; Clara Urzì. A New Extremotolerant Ecotype of the Fungus Pseudotaeniolina globosa Isolated from Djoser Pyramid, Memphis Necropolis, Egypt. Journal of Fungi 2021, 7, 104 .

AMA Style

Samah Mohamed Rizk, Mahmoud Magdy, Filomena De Leo, Olaf Werner, Mohamed Abdel-Salam Rashed, Rosa Maria Ros, Clara Urzì. A New Extremotolerant Ecotype of the Fungus Pseudotaeniolina globosa Isolated from Djoser Pyramid, Memphis Necropolis, Egypt. Journal of Fungi. 2021; 7 (2):104.

Chicago/Turabian Style

Samah Mohamed Rizk; Mahmoud Magdy; Filomena De Leo; Olaf Werner; Mohamed Abdel-Salam Rashed; Rosa Maria Ros; Clara Urzì. 2021. "A New Extremotolerant Ecotype of the Fungus Pseudotaeniolina globosa Isolated from Djoser Pyramid, Memphis Necropolis, Egypt." Journal of Fungi 7, no. 2: 104.

Journal article
Published: 29 January 2021 in Sustainability
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The study evaluates pollution by Pb, Zn, and Cr, and a possible sustainable solution through phytoremediation technologies, in the surroundings of Haina, a very polluted area of the Dominican Republic. Soils and plants were analyzed at 11 sampling points. After sample processing, the elemental composition was analyzed by ICP-OES. Soil metal concentrations, contaminating factors, pollution load indexes, and the Nemerow pollution index were assessed. Soil metal concentrations showed Pb > Zn > Cr, resulting in very strong Pb pollution and medium-impact Zn pollution, with an anthropogenic origin in some sites. This means that some agricultural and residential restrictions must be applied. Accumulation levels in plant tissues, bioaccumulation factors in roots and shoots, and translocation factors were determined for Acalypha alopecuroidea, Achyranthes aspera, Amaranthus dubius, Bidens pilosa, Heliotropium angiospermum, Parthenium hysterophorus, and Sida rhombifolia. The vast majority of the plants showed very low levels of the potentially toxic elements studied, although it may be advisable to take precautions before consumption as they are all considered edible, fodder, and/or medicinal plants. Despite their low rate of bioaccumulation, most of the plants studied could be suitable for the application of phytoremediation of Zn in the field, although further studies are needed to assess their potential for this.

ACS Style

Agripina Ramírez; Gregorio García; Olaf Werner; José Navarro-Pedreño; Rosa Ros. Implications of the Phytoremediation of Heavy Metal Contamination of Soils and Wild Plants in the Industrial Area of Haina, Dominican Republic. Sustainability 2021, 13, 1403 .

AMA Style

Agripina Ramírez, Gregorio García, Olaf Werner, José Navarro-Pedreño, Rosa Ros. Implications of the Phytoremediation of Heavy Metal Contamination of Soils and Wild Plants in the Industrial Area of Haina, Dominican Republic. Sustainability. 2021; 13 (3):1403.

Chicago/Turabian Style

Agripina Ramírez; Gregorio García; Olaf Werner; José Navarro-Pedreño; Rosa Ros. 2021. "Implications of the Phytoremediation of Heavy Metal Contamination of Soils and Wild Plants in the Industrial Area of Haina, Dominican Republic." Sustainability 13, no. 3: 1403.

Bryological notes
Published: 14 September 2020 in Journal of Bryology
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ACS Style

Rosa M. Ros; Olaf Werner; Mohamed Farag; Elena De La Cruz-Martínez. Revision of Bryum glauculum Müll.Hal. nom. nud. reveals the second published record of B. valparaisense Thér. from Egypt. Journal of Bryology 2020, 42, 390 -392.

AMA Style

Rosa M. Ros, Olaf Werner, Mohamed Farag, Elena De La Cruz-Martínez. Revision of Bryum glauculum Müll.Hal. nom. nud. reveals the second published record of B. valparaisense Thér. from Egypt. Journal of Bryology. 2020; 42 (4):390-392.

Chicago/Turabian Style

Rosa M. Ros; Olaf Werner; Mohamed Farag; Elena De La Cruz-Martínez. 2020. "Revision of Bryum glauculum Müll.Hal. nom. nud. reveals the second published record of B. valparaisense Thér. from Egypt." Journal of Bryology 42, no. 4: 390-392.

Methods article
Published: 28 May 2020 in Frontiers in Plant Science
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Reference-free reduced representation bisulfite sequencing uses enzymatic digestion for reducing genome complexity and allows detection of markers to study DNA methylation of a high number of individuals in natural populations of non-model organisms. Current methods like epiGBS enquire the use of a higher number of methylated DNA oligos with a significant cost (especially for small labs and first pilot studies). In this paper, we present a modification of this epiGBS protocol that requires the use of only one hemimethylated P2 (common) adapter, which is combined with unmethylated barcoded adapters. The unmethylated cytosines of one chain of the barcoded adapter are replaced by methylated cytosines using nick translation with methylated cytosines in dNTP solution. The basic version of our technique uses only one restriction enzyme, and as a result, genomic fragments are integrated into two orientations with respect to the adapter sequences. Comparing the sequences of two chain orientations makes it possible to reconstruct the original sequence before bisulfite treatment with the help of standard software and newly developed software written in C and described here. We provide a proof of concept via data obtained from almond (Prunus dulcis). Example data and a detailed description of the complete software pipeline starting from the raw reads up until the final differentially methylated cytosines are given in Supplementary Material making this technique accessible to non-expert computer users. The adapter design showed in this paper should allow the use of a two restriction enzyme approach with minor changes in software parameters.

ACS Style

Olaf Werner; Ángela S. Prudencio; Elena De La Cruz-Martínez; Marta Nieto-Lugilde; Pedro Martínez-Gómez; Rosa M. Ros. A Cost Reduced Variant of Epi-Genotyping by Sequencing for Studying DNA Methylation in Non-model Organisms. Frontiers in Plant Science 2020, 11, 694 .

AMA Style

Olaf Werner, Ángela S. Prudencio, Elena De La Cruz-Martínez, Marta Nieto-Lugilde, Pedro Martínez-Gómez, Rosa M. Ros. A Cost Reduced Variant of Epi-Genotyping by Sequencing for Studying DNA Methylation in Non-model Organisms. Frontiers in Plant Science. 2020; 11 ():694.

Chicago/Turabian Style

Olaf Werner; Ángela S. Prudencio; Elena De La Cruz-Martínez; Marta Nieto-Lugilde; Pedro Martínez-Gómez; Rosa M. Ros. 2020. "A Cost Reduced Variant of Epi-Genotyping by Sequencing for Studying DNA Methylation in Non-model Organisms." Frontiers in Plant Science 11, no. : 694.

Articles
Published: 28 February 2020 in International Journal of Phytoremediation
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The use of ornamental plants for the phytoremediation of potentially toxic elements in polluted soils is an interesting task. It makes possible to combine environmental restoration, re-use of land, and the production of goods and services of economic interest. In this work, in vitro experiments using three cultivars of Chrysanthemum (Asteraceae) were carried out with 0, 300, 600, 900, and 1500 mg/kg of lead concentrations for a period of 12 weeks. The objective was to obtain data about their lead tolerance and bioaccumulation capacity in order to know their potential as phytoremediators in a densely populated Caribbean area of the Dominican Republic with a high concentration of lead in soils. The variations in biomass, root growth as well as accumulation of this element in the plants were measured. The results suggest that the three cultivars have a good potential for phytoextraction at moderate pollution levels, as they showed a good bioaccumulation of lead, which had mild effects on their biomass production and root elongation. Additional studies should be carried out to assess their effectiveness as phytoextractors under field conditions, as well as other alternative uses that could generate esthetic, environmental, and/or economic benefits for tropical areas contaminated by Pb.

ACS Style

Agripina Ramírez; Gregorio García; Olaf Werner; Rosa M. Ros. In vitro lead tolerance and accumulation in three Chrysanthemum cultivars for phytoremediation purposes with ornamental plants. International Journal of Phytoremediation 2020, 22, 1110 -1121.

AMA Style

Agripina Ramírez, Gregorio García, Olaf Werner, Rosa M. Ros. In vitro lead tolerance and accumulation in three Chrysanthemum cultivars for phytoremediation purposes with ornamental plants. International Journal of Phytoremediation. 2020; 22 (11):1110-1121.

Chicago/Turabian Style

Agripina Ramírez; Gregorio García; Olaf Werner; Rosa M. Ros. 2020. "In vitro lead tolerance and accumulation in three Chrysanthemum cultivars for phytoremediation purposes with ornamental plants." International Journal of Phytoremediation 22, no. 11: 1110-1121.

Journal article
Published: 06 December 2018 in The Bryologist
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Tortella mediterranea is described as a new species from the Mediterranean Basin of southern Europe. It is mainly characterized by (1) absence of a stem central strand, (2) lanceolate leaf shape, (3) rather gradual limb-sheath transition, (4) partial presence of elongate marginal cells in the upper half of leaf and (5) ventral costal stereid band usually stronger than the dorsal. Chloroplast rps4 and nuclear ITS sequences were invariable within the studied specimens of the species, suggesting a low genetic variability, and the phylogenetic position is rather isolated with only obscure affinities to the group of closely related taxa around T. tortuosa and to T. fasciculata. The new species, seemingly a rather rare plant, was collected from dry and sunny to shaded limestone rocks. In addition, new molecular data for T. nitida s.str. and putative var. irrigata are provided and taxonomic problems within this variable species are discussed.

ACS Style

Heribert Köckinger; Michael Lüth; Olaf Werner; Rosa M. Ros. Tortella mediterranea (Pottiaceae), a new species from southern Europe, its molecular affinities, and taxonomic notes on T. nitida. The Bryologist 2018, 121, 1 .

AMA Style

Heribert Köckinger, Michael Lüth, Olaf Werner, Rosa M. Ros. Tortella mediterranea (Pottiaceae), a new species from southern Europe, its molecular affinities, and taxonomic notes on T. nitida. The Bryologist. 2018; 121 (4):1.

Chicago/Turabian Style

Heribert Köckinger; Michael Lüth; Olaf Werner; Rosa M. Ros. 2018. "Tortella mediterranea (Pottiaceae), a new species from southern Europe, its molecular affinities, and taxonomic notes on T. nitida." The Bryologist 121, no. 4: 1.

Journal article
Published: 10 November 2018 in International Journal of Molecular Sciences
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DNA methylation and histone post-translational modifications have been described as epigenetic regulation mechanisms involved in developmental transitions in plants, including seasonal changes in fruit trees. In species like almond (Prunus dulcis (Mill.) D.A: Webb), prolonged exposure to cold temperatures is required for dormancy release and flowering. Aiming to identify genomic regions with differential methylation states in response to chill accumulation, we carried out Illumina reduced-representation genome sequencing on bisulfite-treated DNA from floral buds. To do this, we analyzed almond genotypes with different chilling requirements and flowering times both before and after dormancy release for two consecutive years. The study was performed using epi-Genotyping by Sequencing (epi-GBS). A total of 7317 fragments were sequenced and the samples compared. Out of these fragments, 677 were identified as differentially methylated between the almond genotypes. Mapping these fragments using the Prunus persica (L.) Batsch v.2 genome as reference provided information about coding regions linked to early and late flowering methylation markers. Additionally, the methylation state of ten gene-coding sequences was found to be linked to the dormancy release process.

ACS Style

Ángela S. Prudencio; Olaf Werner; Pedro J. Martínez-García; Federico Dicenta; Rosa M. Ros; Pedro Martínez-Gómez. DNA Methylation Analysis of Dormancy Release in Almond (Prunus dulcis) Flower Buds Using Epi-Genotyping by Sequencing. International Journal of Molecular Sciences 2018, 19, 3542 .

AMA Style

Ángela S. Prudencio, Olaf Werner, Pedro J. Martínez-García, Federico Dicenta, Rosa M. Ros, Pedro Martínez-Gómez. DNA Methylation Analysis of Dormancy Release in Almond (Prunus dulcis) Flower Buds Using Epi-Genotyping by Sequencing. International Journal of Molecular Sciences. 2018; 19 (11):3542.

Chicago/Turabian Style

Ángela S. Prudencio; Olaf Werner; Pedro J. Martínez-García; Federico Dicenta; Rosa M. Ros; Pedro Martínez-Gómez. 2018. "DNA Methylation Analysis of Dormancy Release in Almond (Prunus dulcis) Flower Buds Using Epi-Genotyping by Sequencing." International Journal of Molecular Sciences 19, no. 11: 3542.

Journal article
Published: 01 August 2018 in TAXON
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A major problem in taxonomy is to determine if morphological variation in field-collected specimens is caused by genetic differentiation, and therefore corresponds to evolutionary distinct units, or is caused by environmental variation acting on a single interbreeding population. To evaluate the effect of environmental variation on the taxonomy of the moss genus Ceratodon, we compared biometric analyses based on 22 morphological characters on both field-collected plants and cultivated plants to a clustering based on DNA sequence and genome size data published previously. We sampled Ceratodon species from mountainous areas of the Mediterranean region, and other mountain regions and lowlands, mostly from southern Europe. We found that the expression of several gametophytic traits changed between field and laboratory conditions, confirming that environmental variability complicates taxonomic inferences, and suggesting that some characters should be used with caution in distinguishing among species. However, consistent with the genetic and flow cytometry data, we found a clear biometric discontinuity between some plants collected from southern Spain, and those from other parts of the world. Samples considered of hybrid origin, based on genetic data, were morphologically indistinguishable from plants from the southern Spanish mountains. Integrative taxonomy based on genetic, genome size and morphological data unambiguously support the recognition of a new species, Ceratodon amazonum. These data also suggest that the previously recognized C. conicus is a recombinant between C. amazonum and C. purpureus and is considered here to be a nothospecies, for which an epitype is designated because the lectotype is demonstrably ambiguous.

ACS Style

Marta Nieto-Lugilde; Olaf Werner; Stuart F. McDaniel; Rosa M. Ros. Environmental variation obscures species diversity in southern European populations of the moss genus Ceratodon. TAXON 2018, 67, 673 -692.

AMA Style

Marta Nieto-Lugilde, Olaf Werner, Stuart F. McDaniel, Rosa M. Ros. Environmental variation obscures species diversity in southern European populations of the moss genus Ceratodon. TAXON. 2018; 67 (4):673-692.

Chicago/Turabian Style

Marta Nieto-Lugilde; Olaf Werner; Stuart F. McDaniel; Rosa M. Ros. 2018. "Environmental variation obscures species diversity in southern European populations of the moss genus Ceratodon." TAXON 67, no. 4: 673-692.

Research article
Published: 29 June 2018 in American Journal of Botany
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Premise of the Study A period of allopatry is widely believed to be essential for the evolution of reproductive isolation. However, strict allopatry may be difficult to achieve in some cosmopolitan, spore‐dispersed groups, like mosses. We examined the genetic and genome size diversity in Mediterranean populations of the moss Ceratodon purpureus s.l. to evaluate the role of allopatry and ploidy change in population divergence. Methods We sampled populations of the genus Ceratodon from mountainous areas and lowlands of the Mediterranean region, and from Western and Central Europe. We performed phylogenetic and coalescent analyses on sequences from five nuclear introns and a chloroplast locus to reconstruct their evolutionary history. We also estimated genome size using flow cytometry (employing propidium iodide) and determined the sex of samples using a sex‐linked PCR marker. Key Results Two well‐differentiated clades were resolved, discriminating two homogeneous groups: the widespread C. purpureus and a local group mostly restricted to the mountains in Southern Spain. The latter also possessed a genome size 25% larger than the widespread C. purpureus, and the samples of this group consist entirely of females. We also found hybrids, and some of them had a genome size equivalent to the sum of the C. purpureus and Spanish genome, suggesting that they arose by allopolyploidy. Conclusions These data suggest that a new species of Ceratodon arose via peripatric speciation, potentially involving a genome size change and a strong female‐biased sex ratio. The new species has hybridized in the past with C. purpureus.

ACS Style

Marta Nieto-Lugilde; Olaf Werner; Stuart F. McDaniel; Petr Koutecký; Jan Kučera; Samah Mohamed Rizk; Rosa M. Ros. Peripatric speciation associated with genome expansion and female‐biased sex ratios in the moss genus Ceratodon. American Journal of Botany 2018, 105, 1009 -1020.

AMA Style

Marta Nieto-Lugilde, Olaf Werner, Stuart F. McDaniel, Petr Koutecký, Jan Kučera, Samah Mohamed Rizk, Rosa M. Ros. Peripatric speciation associated with genome expansion and female‐biased sex ratios in the moss genus Ceratodon. American Journal of Botany. 2018; 105 (6):1009-1020.

Chicago/Turabian Style

Marta Nieto-Lugilde; Olaf Werner; Stuart F. McDaniel; Petr Koutecký; Jan Kučera; Samah Mohamed Rizk; Rosa M. Ros. 2018. "Peripatric speciation associated with genome expansion and female‐biased sex ratios in the moss genus Ceratodon." American Journal of Botany 105, no. 6: 1009-1020.

Journal article
Published: 01 April 2018 in Cryptogamie, Bryologie
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ACS Style

Marta Nieto-Lugilde; Olaf Werner; Rosa M. Ros. Taxonomical and Nomenclatural Notes on the Moss Ceratodon conicus (Ditrichaceae, Bryophyta). Cryptogamie, Bryologie 2018, 39, 195 -200.

AMA Style

Marta Nieto-Lugilde, Olaf Werner, Rosa M. Ros. Taxonomical and Nomenclatural Notes on the Moss Ceratodon conicus (Ditrichaceae, Bryophyta). Cryptogamie, Bryologie. 2018; 39 (2):195-200.

Chicago/Turabian Style

Marta Nieto-Lugilde; Olaf Werner; Rosa M. Ros. 2018. "Taxonomical and Nomenclatural Notes on the Moss Ceratodon conicus (Ditrichaceae, Bryophyta)." Cryptogamie, Bryologie 39, no. 2: 195-200.

Journal article
Published: 01 April 2018 in Cryptogamie, Bryologie
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ACS Style

Jan Kučera; Tom L. Blockeel; Peter Erzberger; Beáta Papp; Zdenêk Soldán; Kai Vellak; Olaf Werner; Rosa M. Ros. TheDidymodon tophaceusComplex (Pottiaceae, Bryophyta) Revisited: New Data Support the Subspecific Rank of Currently Recognized Species. Cryptogamie, Bryologie 2018, 39, 241 -257.

AMA Style

Jan Kučera, Tom L. Blockeel, Peter Erzberger, Beáta Papp, Zdenêk Soldán, Kai Vellak, Olaf Werner, Rosa M. Ros. TheDidymodon tophaceusComplex (Pottiaceae, Bryophyta) Revisited: New Data Support the Subspecific Rank of Currently Recognized Species. Cryptogamie, Bryologie. 2018; 39 (2):241-257.

Chicago/Turabian Style

Jan Kučera; Tom L. Blockeel; Peter Erzberger; Beáta Papp; Zdenêk Soldán; Kai Vellak; Olaf Werner; Rosa M. Ros. 2018. "TheDidymodon tophaceusComplex (Pottiaceae, Bryophyta) Revisited: New Data Support the Subspecific Rank of Currently Recognized Species." Cryptogamie, Bryologie 39, no. 2: 241-257.

Journal article
Published: 01 December 2017 in Annals of Agricultural Sciences
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ACS Style

J.K. Ahiakpa; Mahmoud Magdy; Olaf Werner; H.M. Amoatey; M.A. Yeboah; A.S. Appiah; E.K Quartey; Rosa M. Ros. Intra-specific variation in West African and Asian germplasm of okra ( Abelmoschus spp L.). Annals of Agricultural Sciences 2017, 62, 131 -138.

AMA Style

J.K. Ahiakpa, Mahmoud Magdy, Olaf Werner, H.M. Amoatey, M.A. Yeboah, A.S. Appiah, E.K Quartey, Rosa M. Ros. Intra-specific variation in West African and Asian germplasm of okra ( Abelmoschus spp L.). Annals of Agricultural Sciences. 2017; 62 (2):131-138.

Chicago/Turabian Style

J.K. Ahiakpa; Mahmoud Magdy; Olaf Werner; H.M. Amoatey; M.A. Yeboah; A.S. Appiah; E.K Quartey; Rosa M. Ros. 2017. "Intra-specific variation in West African and Asian germplasm of okra ( Abelmoschus spp L.)." Annals of Agricultural Sciences 62, no. 2: 131-138.

Preprint
Published: 30 November 2017
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PREMISE OF THE STUDYA period of allopatry is widely believed to be essential for the evolution of reproductive isolation. However, strict allopatry may be difficult to achieve in some cosmopolitan, spore-dispersed groups, like mosses. Here we examine the genetic and genome size diversity in Mediterranean populations of the moss Ceratodon purpureus s.l. to evaluate the role of allopatry and ploidy change in population divergence.METHODSWe sampled populations of the genus Ceratodon from mountainous areas and lowlands of the Mediterranean region, and from western and central Europe. We performed phylogenetic and coalescent analyses on sequences from five nuclear introns and a chloroplast locus to reconstruct their evolutionary history. We also estimated the genome size using flow cytometry, employing propidium iodide, and determined their sex using a sex-linked PCR marker.KEY RESULTSTwo well differentiated clades were resolved, discriminating two homogeneous groups: the widespread C. purpureus and a local group mostly restricted to the mountains in southern Spain. The latter also possessed a genome size 25% larger than the widespread C. purpureus, and the samples of this group consist entirely of females. We also found hybrids, and some of them had a genome size equivalent to the sum of the C. purpureus and Spanish genome, suggesting that they arose by allopolyploidy.CONCLUSIONSThese data suggest that a new species of Ceratodon arose via peripatric speciation, potentially involving a genome size change and a strong female-biased sex ratio. The new species has hybridized in the past with C. purpureus.

ACS Style

Marta Nieto-Lugilde; Olaf Werner; Stuart F. McDaniel; Petr Koutecký; Jan Kučera; Samah Mohamed Rizk; Rosa M. Ros. Peripatric speciation associated with genome expansion and female-biased sex ratios in the moss genus Ceratodon. 2017, 227280 .

AMA Style

Marta Nieto-Lugilde, Olaf Werner, Stuart F. McDaniel, Petr Koutecký, Jan Kučera, Samah Mohamed Rizk, Rosa M. Ros. Peripatric speciation associated with genome expansion and female-biased sex ratios in the moss genus Ceratodon. . 2017; ():227280.

Chicago/Turabian Style

Marta Nieto-Lugilde; Olaf Werner; Stuart F. McDaniel; Petr Koutecký; Jan Kučera; Samah Mohamed Rizk; Rosa M. Ros. 2017. "Peripatric speciation associated with genome expansion and female-biased sex ratios in the moss genus Ceratodon." , no. : 227280.

Articles
Published: 01 July 2016 in Journal of Bryology
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Grimmia curviseta Bouman is a moss species endemic to the Canary Islands in the North Atlantic Ocean, which has a very restricted distribution across the summit areas of Tenerife and La Palma islands. Using massive sequencing, we developed ten polymorphic microsatellite markers for this species. The pattern of microsatellite alleles per locus provides preliminary evidence that G. curviseta is allodiploid. The average number of alleles per locus ranged from 2 to 7, and observed heterozygosities varied from 0.022 to 1.000. A significant genetic differentiation was observed between the Tenerife and La Palma populations, suggesting that there are some limitations to dispersal. This set of microsatellites constitutes a valuable tool to investigate patterns of genetic diversity across the distribution range of G. curviseta, information that may eventually be used to establish conservation strategies for the species. Owing to the cross-amplification with the closely related species Grimmia montana Bruch & Schimp., our study also demonstrates the utility of these markers for population-level genetic analyses in the genus Grimmia.

ACS Style

Alexandra Rodríguez-Romero; Mariano Hernandez; Jairo Patiño; Olaf Werner; Juana María González-Mancebo. Characterization of ten polymorphic microsatellite loci for the threatened species Grimmia curviseta Bouman (Grimmiaceae, Musci). Journal of Bryology 2016, 39, 16 -22.

AMA Style

Alexandra Rodríguez-Romero, Mariano Hernandez, Jairo Patiño, Olaf Werner, Juana María González-Mancebo. Characterization of ten polymorphic microsatellite loci for the threatened species Grimmia curviseta Bouman (Grimmiaceae, Musci). Journal of Bryology. 2016; 39 (1):16-22.

Chicago/Turabian Style

Alexandra Rodríguez-Romero; Mariano Hernandez; Jairo Patiño; Olaf Werner; Juana María González-Mancebo. 2016. "Characterization of ten polymorphic microsatellite loci for the threatened species Grimmia curviseta Bouman (Grimmiaceae, Musci)." Journal of Bryology 39, no. 1: 16-22.

Journal article
Published: 01 May 2016 in Journal for Nature Conservation
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Nostoc flagelliforme has been reported from deserts of all continents and more recently from semiarid environments in south-east Spain and Australia. Its cylindrical thalli are very conspicuous on soils after rains and can be easily differentiated from other taxa and it is considered endangered in some countries, e. g. China. It was firstly described as variety flagelliforme of Nostoc commune but later was considered a separate species. The morphology, fine structure and ecology of populations of both taxa from Australia and different regions of Spain were studied and 16S rRNA and trnLLeu (UAA) genes were sequenced to identify their intraspecific genetic variation. The morphological study revealed the presence of several intermediate morphs, from spheres and sheets to ribbons followed by the cylindrical thalli and the overlapping of cell dimensions, permitting the differentiation of cylindrical morphs from the others. The molecular data did not allow for a distinction between N. commune and N. flagelliforme which is clearly polyphyletic and both show a high genetic diversity. The presence of cylindrical thalli seems to be related to extreme conditions of aridity, usually on soils but occasionally on rock walls. The ecotype that N. flagelliforme represents deserves to be protected for itself and for the evolutionary process it represents opening up the possibility of implementation of recovery measures and even opportunities to explore its biotechnological production from a different point of view.

ACS Style

Marina Aboal; Olaf Werner; Maria Eugenia García-Fernández; José Antonio Palazón; José Carlos Cristóbal; Wendy Williams. Should ecomorphs be conserved? The case of Nostoc flagelliforme, an endangered extremophile cyanobacteria. Journal for Nature Conservation 2016, 30, 52 -64.

AMA Style

Marina Aboal, Olaf Werner, Maria Eugenia García-Fernández, José Antonio Palazón, José Carlos Cristóbal, Wendy Williams. Should ecomorphs be conserved? The case of Nostoc flagelliforme, an endangered extremophile cyanobacteria. Journal for Nature Conservation. 2016; 30 ():52-64.

Chicago/Turabian Style

Marina Aboal; Olaf Werner; Maria Eugenia García-Fernández; José Antonio Palazón; José Carlos Cristóbal; Wendy Williams. 2016. "Should ecomorphs be conserved? The case of Nostoc flagelliforme, an endangered extremophile cyanobacteria." Journal for Nature Conservation 30, no. : 52-64.

Journal article
Published: 08 September 2015 in Plant Biology
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The common cord moss Funaria hygrometrica has a worldwide distribution and thrives in a wide variety of environments. Here, we studied the genetic diversity in F. hygrometrica along an abiotic gradient in the Mediterranean high mountain of Sierra Nevada (Spain) using a genome scan method. Eighty-four samples from 17 locations from 24 to 2700 m were fingerprinted based on their amplified fragment length polymorphism (AFLP) banding pattern. Using PCA and Bayesian inference we found that the genetic diversity was structured in three or four clusters, respectively. Using a genome scan method we identified 13 outlier loci, which showed a signature of positive selection. Partial Mantel tests were performed between the Euclidean distance matrices of geographic and climatic variables, versus the pair-wise genetic distance of the AFLP dataset and AFLP-positive outliers dataset. AFLP-positive outlier data were significantly correlated with the gradient of the climatic variables, suggesting adaptive variation among populations of F. hygrometrica along the Sierra Nevada Mountains. We highlight the additional analyses necessary to identify the nature of these loci, and their biological role in the adaptation process.

ACS Style

Mahmoud Magdy; Olaf Werner; S. F. McDaniel; B. Goffinet; Rosa María Ros-Espin. Genomic scanning using AFLP to detect loci under selection in the mossFunaria hygrometricaalong a climate gradient in the Sierra Nevada Mountains, Spain. Plant Biology 2015, 18, 280 -288.

AMA Style

Mahmoud Magdy, Olaf Werner, S. F. McDaniel, B. Goffinet, Rosa María Ros-Espin. Genomic scanning using AFLP to detect loci under selection in the mossFunaria hygrometricaalong a climate gradient in the Sierra Nevada Mountains, Spain. Plant Biology. 2015; 18 (2):280-288.

Chicago/Turabian Style

Mahmoud Magdy; Olaf Werner; S. F. McDaniel; B. Goffinet; Rosa María Ros-Espin. 2015. "Genomic scanning using AFLP to detect loci under selection in the mossFunaria hygrometricaalong a climate gradient in the Sierra Nevada Mountains, Spain." Plant Biology 18, no. 2: 280-288.

Journal article
Published: 22 June 2015 in Plant Biology
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The distinction between native and introduced biotas presents unique challenges that culminate in organisms with high long-distance dispersal capacities in a rapidly changing world. Bryophytes, in particular, exhibit large distribution ranges, and some species can truly be qualified as cosmopolitan. Cosmopolitan species, however, typically occur in disturbed environments, raising the question of their nativeness throughout their range. Here, we employ genetic data to address the question of the origin of the cosmopolitan, weedy moss Bryum argenteum on the island of Tenerife. The genetic diversity of B. argenteum on Tenerife was comparable to that found in continental areas due to recurrent colonisation events, erasing any signature of a bottleneck that would be expected in the case of a recent colonisation event. The molecular dating analyses indicated that the first colonisation of the island took place more than 100,000 years ago, i.e. well before the first human settlements. Furthermore, the significant signal for isolation-by-distance found in B. argenteum within Tenerife points to the substantial role of genetic drift in establishing the observed patterns of genetic variation. Together, the results support the hypothesis that B. argenteum is native on Tenerife; although the existence of haplotypes shared between Tenerife and continental areas suggests that more recent, potentially man-mediated introduction also took place. While defining nativeness in organisms that are not deliberately introduced, and wherein the fossil record is extremely scarce, is an exceedingly challenging task, our results suggest that population genetic analyses can represent a useful tool to help distinguish native from alien populations.

ACS Style

S. Pisa; A. Vanderpoorten; Jairo Patiño; Olaf Werner; Juana María González-Mancebo; R. M. Ros. How to define nativeness in vagile organisms: lessons from the cosmopolitan mossBryum argenteumon the island of Tenerife (Canary Islands). Plant Biology 2015, 17, 1057 -1065.

AMA Style

S. Pisa, A. Vanderpoorten, Jairo Patiño, Olaf Werner, Juana María González-Mancebo, R. M. Ros. How to define nativeness in vagile organisms: lessons from the cosmopolitan mossBryum argenteumon the island of Tenerife (Canary Islands). Plant Biology. 2015; 17 (5):1057-1065.

Chicago/Turabian Style

S. Pisa; A. Vanderpoorten; Jairo Patiño; Olaf Werner; Juana María González-Mancebo; R. M. Ros. 2015. "How to define nativeness in vagile organisms: lessons from the cosmopolitan mossBryum argenteumon the island of Tenerife (Canary Islands)." Plant Biology 17, no. 5: 1057-1065.

Journal article
Published: 09 May 2015 in Genetic Resources and Crop Evolution
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The genus Abelmoschus includes several crop plants which are especially important in SE Asia and several African countries. However, the systematic treatment of this genus is difficult, in part because hybridization between different forms seems to be frequent. In this study we present nuclear internal transcribed spacer ITS and chloroplast rpL16 sequences with the aim of reconstructing phylogenetic relationships within Abelmoschus, and its relationship with the genus Hibiscus and other related Malvaceae. Based on our analysis of nuclear ITS and chloroplast rpL16 sequence data, Abelmoschus is resolved as a monophyletic clade. Abelmoschus tetraphyllus is clearly separated from A. manihot but closely related to A. ficulneus and should not be treated as a subspecies of A. manihot. None of the wild species included in this study can be confirmed as an ancestor of A. esculentus or A. caillei. Neither A. esculentus nor A. caillei can be distinguished from each other by the markers used for this study, although the evidence does not exclude the possibility of a hybrid origin of A. caillei involving A. esculentus and an unknown species. The genetic diversity within A. esculentus and A. caillei is low if compared with A. manihot. The evidence presented here does not allow us to draw any conclusions about the geographic origin (Africa vs. Asia) of A. esculentus.

ACS Style

Olaf Werner; Mahmoud Magdy; Rosa M. Ros. Molecular systematics of Abelmoschus (Malvaceae) and genetic diversity within the cultivated species of this genus based on nuclear ITS and chloroplast rpL16 sequence data. Genetic Resources and Crop Evolution 2015, 63, 429 -445.

AMA Style

Olaf Werner, Mahmoud Magdy, Rosa M. Ros. Molecular systematics of Abelmoschus (Malvaceae) and genetic diversity within the cultivated species of this genus based on nuclear ITS and chloroplast rpL16 sequence data. Genetic Resources and Crop Evolution. 2015; 63 (3):429-445.

Chicago/Turabian Style

Olaf Werner; Mahmoud Magdy; Rosa M. Ros. 2015. "Molecular systematics of Abelmoschus (Malvaceae) and genetic diversity within the cultivated species of this genus based on nuclear ITS and chloroplast rpL16 sequence data." Genetic Resources and Crop Evolution 63, no. 3: 429-445.

Journal article
Published: 01 May 2015 in Nova Hedwigia
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ACS Style

Olaf Werner; Aurora Rodríguez-Atienza; Marta Nieto-Lugilde; Rosa María Ros-Espin. New data on the systematics of the European-Asian species of Leucodon (Leucodontaceae, Bryophyta). Nova Hedwigia 2015, 100, 333 -354.

AMA Style

Olaf Werner, Aurora Rodríguez-Atienza, Marta Nieto-Lugilde, Rosa María Ros-Espin. New data on the systematics of the European-Asian species of Leucodon (Leucodontaceae, Bryophyta). Nova Hedwigia. 2015; 100 (3-4):333-354.

Chicago/Turabian Style

Olaf Werner; Aurora Rodríguez-Atienza; Marta Nieto-Lugilde; Rosa María Ros-Espin. 2015. "New data on the systematics of the European-Asian species of Leucodon (Leucodontaceae, Bryophyta)." Nova Hedwigia 100, no. 3-4: 333-354.