Understanding how the soil environment impacts root water uptake location and magnitude is important for better management of plant irrigation. In this study, stable hydrogen and oxygen isotope composition were used to determine seasonal variations in the depth of water taken up by walnut trees during different phenological stages in an irrigated arid hilly area in the Taihang Mountains in China. The contributions of soil water at different depths to the water taken up were quantified by the MixSIAR Bayesian isotope mixing model. The results indicated that water taken up by the walnut trees was sourced mainly from soil water in the 0–20 cm soil layer at the sprouting and leaf expansion stages (62.95%), and the 20–40 cm soil layer at blossoming and fruit-bearing (43.45%), fruit expansion (41.8%), and fruit maturity (39.15%) stages. The mean soil depth of the water taken up by the walnut trees gradually decreased as the phenological stages advanced. The proportions of various soil layer water contributions to the walnut trees differed throughout the phenological stages, and the proportion of deeper soil water contributions gradually increased as the phenological stages of walnut trees advanced. The results of the present study indicated that water sources for walnut trees varied by depth during different phenological stages. In addition to soil moisture, soil temperature may also be an important factor affecting the depth of water taken up by walnut trees. The results also provided scientific implications for water-saving irrigation management.

The walnut is an important nut that has numerous uses worldwide. However, due to dwarf and close plantation methods as well as continuous cloudy or rainy days that occur during periods of walnut oil accumulation, the walnut fruit exhibits varying degrees of stress under low-light conditions. However, the effects of shade on metabolites and genes in walnut embryos remain unclear in the literature. The purpose of this study is to investigate the lipid biosynthesis process that occurs in walnut embryos under shade treatment via the use of metabolomics and transcriptomics analyses. The results indicate that the oil content decreases significantly under shaded conditions, while the protein content increases significantly. The expression levels of fatty acid desaturase 2 (FAD2) and stearoyl-ACP-desaturase (SAD) involved in the lipid biosynthesis mechanism were significantly reduced in the shaded group, which resulted in reductions in oleic (C18:1), linoleic (C18:2), and α-linolenic (C18:3) acids. The reduced oil content was consistent with the downregulation of genes associated with the lipid biosynthesis mechanism. In the amino acid biosynthesis process, the upregulated cysteine synthase (cscK) and anthranilate synthase beta subunit 2 (trpG) genes promoted the accumulation of L-aspartic acid and L-citrulline. The increase in protein content was consistent with the upregulation of genes related to amino acid biosynthesis. Thus, our study provides new insights into the regulatory mechanisms of shade underlying overall walnut fruit quality.