Systemic lupus erythematosus (SLE) and systemic sclerosis (SSc) are systemic autoimmune connective tissue diseases that share overlapping clinico-pathological features. It is highly probable that there is an overlap in epigenetic landscapes of both diseases. This study aimed to identify similarities in DNA methylation changes in genes involved in SLE and SSc. Global DNA methylation and twelve genes selected on the basis of their involvement in inflammation, autoimmunity and/or fibrosis were analyzed using PCR arrays in three groups, each of 30 Black South Africans with SLE and SSc, plus 40 healthy control subjects. Global methylation in both diseases was significantly lower (30 %, p = 0.0000001). In comparison to healthy controls, a similar gene-specific methylation pattern was observed in both SLE and SSc. Three genes, namely; PRF1, ITGAL and FOXP3 were consistently hypermethylated while CDKN2A and CD70 were hypomethylated in both diseases. The other genes (SOCS1, CTGF, THY1, CXCR4, MT1-G, FLI1, and DNMT1) were generally hypomethylated in SLE whereas they were neither hyper- nor hypo-methylated in SSc. SSc and SLE patients have a higher global hypomethylation than healthy subjects with specific genes being hypomethylated and others hypermethylated. The majority of genes studied were hypomethylated in SLE compared to SSc. In addition to the commonly known hypomethylated genes in SLE and SSc, there are other hypomethylated genes (such as MT-1G and THY-1) that have not previously been investigated in SLE and SSc though are known to be hypermethylated in cancer.

Since the outbreak of the Coronavirus Disease 2019 (CoViD-19), the World Health Organization has recommended that, in absence of soap and water, alcohol-based hand sanitizer can be used to prevent the transmission of coronaviruses. Unfortunately, many media and anecdotal reports indicate that many alcohol-based hand sanitizers sold in South Africa are substandard and some contain potentially toxic ingredients. The study aimed to identify hand sanitizers used in the Johannesburg area during the CoViD-19 pandemic that do not contain the recommended alcohol concentration of at least 70% propanol or 60% ethanol, and contain traces of toxic ingredients. Hand sanitizers randomly collected from various traders around Johannesburg were analyzed using Agilent auto sampler coupled to a gas chromatograph utilizing flame ionisation detection. Of the 94 hand sanitizer samples collected, three preparations contained no alcohol, whereas the rest contained either ethanol, 2-propanol or 1-propanol or a combination of two alcohols. Of the alcohol-containing hand sanitizers, 37 (41%) contained less than 60% alcohol. Ethyl acetate, isobutanol and other non-recommended alcohols (methanol and 3-methyl-butanol) were also identified. Consumers are therefore warned that among the many brands of hand sanitizers found around Johannesburg, there are some substandard preparations and some that contain traces of toxic ingredients.

Background: This study aimed at assessing breast cancer awareness among women presenting with newly diagnosed breast disease at Universitas Hospital in Bloemfontein, South Africa. The breast cancer awareness of the women, in turn, was related to their screening practices and the stage of breast cancer at presentation. Recommendations to address the clinical implications of the findings of this study were then suggested. Methods: Reports of data on the number of breast cancer awareness campaigns held and the number of people reached during the period April to June 2006 were acquired from the main campaign organiser, CANSA. Data were also obtained by means of interviewer-administered structured questionnaires, from consenting women (n = 56) presenting with newly diagnosed breast disease at Universitas Hospital during the period May 2006 to April 2007. The study was approved by the Ethics Committee of the Faculty of Health Sciences of the University of the Free State. Results: Despite aggressive nationwide public education on breast cancer and on the benefits of screening by CANSA breast cancer awareness, the examination and screening practices of women presenting with newly diagnosed breast disease at the Universitas Hospital were generally low. Most of the women interviewed presented with advanced breast cancer (stage 2 and 3), and reported that their healthcare professionals never initiated clinical breast examinations or mammograms. Conclusion: This study has revealed low breast cancer awareness among women presenting with newly diagnosed breast disease at the Universitas Hospital, and hence low rates of self- and clinical examinations of the breast and low mammographic screening rates. This may be attributed to a general lack of awareness of the rising incidence of breast cancer in the Free State among both the public and healthcare professionals.

This report is an overview of requests for biological and environmental monitoring of hazardous chemicals, submitted to the National Institute for Occupational Health, Analytical Services Laboratory for testing from the years 2005 to 2015. The report discusses the nature of tests requested and implications for workers’ health and environment, as well as potential impact of the uncertainties associated with monitoring of hazardous chemicals. This is a retrospective, descriptive, qualitative and quantitative audit of all samples received and tests performed retrieved from records of analysis by the laboratory. The study sample consisted of 44,221 samples. The report indicates that throughout the interrogation period the demand for biological monitoring was higher than that for environmental monitoring, with more requests for toxic metals than organic pollutants. Toxic metal testing was highest for mercury, followed by manganese, lead, aluminium and arsenic. The highest number of tests for organic pollutants was conducted for pesticides followed by toluene and xylene. The study has also revealed that the scope of tests requested is rather narrow and does not reflect the broad spectrum of South Africa’s industrial diversity. Having identified possible reasons for underutilization, a number of reforms that could enhance the laboratory’s performance have been addressed.

This report is an overview of requests for biological and environmental monitoring of hazardous chemicals, submitted to the National Institute for Occupational Health, Analytical Services Laboratory for testing from the years 2005 to 2015. The report discusses the nature of tests requested and implications for workers’ health and environment, as well as potential impact of the uncertainties associated with monitoring of hazardous chemicals. This is a retrospective, descriptive, qualitative and quantitative audit of all samples received and tests performed retrieved from records of analysis by the laboratory. The study sample consisted of 44,221 samples. The report indicates that throughout the interrogation period the demand for biological monitoring was higher than that for environmental monitoring, with more requests for toxic metals than organic pollutants. Toxic metal testing was highest for mercury, followed by manganese, lead, aluminium and arsenic. The highest number of tests for organic pollutants was conducted for pesticides followed by toluene and xylene. The study has also revealed that the scope of tests requested is rather narrow and does not reflect the broad spectrum of South Africa’s industrial diversity. Having identified possible reasons for underutilization, a number of reforms that could enhance the laboratory’s performance have been addressed.

Since the outbreak of the Coronavirus Disease 2019 (CoViD-19), the World Health Organization has recommended that, in the absence of soap and water, alcohol-based hand sanitizer can be used to prevent the transmission of coronaviruses. Unfortunately, many media reports indicate that majority of current alcohol-based hand sanitizers are substandard and some contain potentially toxic ingredients. The study aimed to identify sanitizers used in the Johannesburg area that do not contain the WHO-recommended alcohol concentration of at least 70% propanol or 60% ethanol, and contain traces of toxic ingredients. Hand sanitizers were randomly collected from various traders around Johannesburg. The samples were analyzed using Agilent Auto sampler coupled to a gas chromatograph utilizing flame ionisation detection. Of the 94 different hand sanitizers collected, three preparations were found to contain no alcohol, whereas the rest contained either ethanol or 2-propanol or a combination of the two. Of the alcohol-containing sanitizers, 37 (41%) contained less than 60% v/v alcohol. Ethyl acetate, isobutanol and other non-recommended alcohols (methanol, 1-propanol and 3-methyl-butanol) were also identified. Consumers are therefore warned that among the many brands of hand sanitizer found around Johannesburg, there are some substandard preparations and some that contain traces of toxic ingredients.