In this in vitro study, the influence of the concentration of abrasive particles on the abrasivity of toothpastes was investigated using laser scan profilometry on polymethyl methacrylate (PMMA) surfaces with the aim of providing an alternative method to developers for screening of new toothpaste formulations. PMMA plates were tested in a toothbrush simulator with distilled water and four model toothpastes with increasing content of hydrated silica (2.5, 5.0, 7.5, 10.0 wt%). The viscosity of the model toothpaste formulations was kept constant by means of varying the content of sodium carboxymethyl cellulose and water. The brushed surfaces were evaluated using laser scan profilometry at micrometer-scale resolutions, and the total volume of the introduced scratches was calculated along with the roughness parameters Ra, Rz and Rv. RDA measurements commissioned for the same toothpaste formulations were used to analyze the correlation between results obtained with the different methods. The same experimental procedure was applied to five commercially available toothpastes, and the results were evaluated against our model system. In addition, we characterize abrasive hydrated silica and discuss their effects on PMMA-sample surfaces. The results show that the abrasiveness of a model toothpaste increases with the weight percentage of hydrated silica. Increasing roughness parameter and volume loss values show good correlation with the likewise increasing corresponding RDA values for all model toothpastes, as well as commercial toothpastes without ingredients that can damage the used substrate PMMA. From our results, we deduce an abrasion classification that corresponds to the RDA classification established for marketed toothpastes.

Modern toothpastes are complex formulations with various ingredients. The aim of this study was to analyze the improvement of toothpaste formulations from 1900 to 2023 focusing on active ingredients with remineralizing, antibacterial, or plaque-removing effects, and to discuss their influence on caries prevention. For this, worldwide patent applications were searched using the international database Espacenet from the European Patent Office. Additionally, toothpaste products were searched using the Mintel product database from 1996 to 2023. The searched ingredients were (in alphabetical order): calcium carbonate, calcium phosphates, hydrated silica, sodium fluoride, sodium lauryl sulfate, triclosan, xylitol, and zinc salts as they are known from the scientific literature to be remineralizing or antibacterial/antiplaque agents. It was shown that the number of patent applications containing these ingredients significantly increased since the 1970s. As these ingredients have remineralizing, antibacterial, or plaque-removing effects, they all can contribute to caries prevention. In conclusion, and within the limitations of this approach, this study shows that toothpaste formulations have greatly improved over the past decades by using various active anticaries ingredients.

Background: There is high demand for white and healthy teeth. Therefore, various whitening toothpastes are commercially available. Hydroxyapatite and blue covarine are two ingredients used in whitening toothpaste formulations. Objectives: This in vitro study analyzed the stain-removing efficacy of two commercial whitening toothpastes: one containing hydroxyapatite and the other containing blue covarine. Methods: The stain-removing efficacy of both toothpastes was analyzed for different brushing times (30 and 180 s) using a staining model on human enamel. Photographic documentation and colorimetric measurements were performed after staining and after each brushing series. Colorimetric measurements were used to determine the stain removal efficacy in percentage. Statistical analysis was performed using one-way analysis of variance (ANOVA) with a post hoc Tukey test and Levene’s test to assess the homogeneity of variances. The significance level α was set at 0.05. Results: The hydroxyapatite toothpaste demonstrated a significantly higher stain-removing efficacy compared to the blue covarine toothpaste after 30 s of brushing. The stain-removing efficacy was comparable between the two toothpastes after 180 s of brushing. Conclusions: Both hydroxyapatite and blue covarine toothpastes effectively cleaned stained enamel, with hydroxyapatite toothpaste showing a significantly higher stain-removing efficacy after 30 s of brushing.

Hypophosphites (also known as phosphinates) are the salts of hypophosphorous acid (also known as phosphinic acid), H₃PO₂. Various hypophosphite salts are known such as calcium hypophosphite (Ca(H₂PO₂)₂) and sodium hypophosphite (Na(H₂PO₂)). Hypophosphites were proposed as a potential treatment for tuberculosis as early as the 1850s; however, they were found to be ineffective against this disease. Following this, there was a period of around 100 years during which no new studies on hypophosphites were published. Subsequent in vitro studies have shown that hypophosphites can be used as potential antibacterial food preservatives. Currently, calcium hypophosphite is used in commercial food supplements for children, as this compound is a suitable source of calcium ions. It also has other advantageous properties, including exceptionally high water solubility (154 g/L at 25 °C), a neutral taste, a high mass fraction of calcium per molecule (23.6%), and an excellent safety profile. Recent studies have shown its potential as an active ingredient in the field of oral care. Since biological mechanisms such as tooth and bone formation and natural remineralization due to saliva rely on calcium ions, calcium hypophosphite can be regarded as a biomimetic agent. Upon contact with phosphate from saliva, calcium hypophosphite forms hydroxyapatite; this imitation of physiological mineralization and crystallization processes in the human body further underlines its biomimetic character. This review summarizes and discusses the available literature on hypophosphites in human health and related fields.

Background: Tooth enamel is the hardest and most highly mineralized tissue in the human body. It serves as a protective barrier against chemical, mechanical, and microbial challenges. Despite its durability, enamel remains vulnerable to developmental and posteruptive defects such as fluorosis, hypomineralization, and amelogenesis imperfecta (AI). For oral health professionals, a clear understanding of the biological and molecular mechanisms underlying enamel formation is essential for advancing preventive and therapeutic strategies in clinical practice. This review synthesizes current knowledge on enamel formation, with emphasis on its cellular, molecular, and structural determinants, and discusses clinically relevant disruptions as well as emerging biomimetic approaches. Methods: This scoping review was conducted according to the PRISMA-ScR guidelines. A systematic literature search of the mechanisms of enamel formation was performed via Embase and Medline. Titles and abstracts were screened independently by three authors. Studies that primarily addressed enamel defects were excluded from the systematic synthesis; however, these studies were retained for narrative discussion. Following the screening process, 92 publications met the inclusion criteria and were incorporated into the thematic synthesis. Results: Enamel formation is a complex, multistage process involving epithelial–mesenchymal interactions and the sequential activity of ameloblasts during presecretory, secretory, transition, and maturation stages. Key mechanisms include the secretion of enamel matrix proteins (e.g., amelogenin, ameloblastin, and enamelin), proteolytic processing by enzymes such as MMP20 and KLK4, and controlled ion transport, leading to hydroxyapatite crystal growth and organization into rod and interrod structures. The structural arrangement endows enamel with exceptional mechanical resistance. Narrative sections address “What can go wrong?”, summarizing genetic, epigenetic, and environmental causes of fluorosis, hypomineralization, and amelogenesis imperfecta, and other developmental defects, whereas “What can we learn from nature?” highlights biomimetic strategies. Conclusions: Human enamel formation is a highly coordinated biomineralization process regulated at the cellular, structural, and molecular levels. Disruptions in these processes underlie major enamel pathologies. Integrating mechanistic insights from natural enamel development with emerging biomimetic technologies offers promising avenues for prevention, diagnosis, and treatment in dentistry. This review provides oral health professionals with a biologically grounded framework to guide evidence-based management of enamel-related conditions.