Introduction: This paper presents an innovative air sampling and rapid detection system for airborne bacteria in the environment. Objective: The proposed system aims to enhance the efficiency and accuracy of airborne bacterial detection by integrating an improved air sampler with a rapid nucleic acid amplification method. Methods: The system incorporates an air sampler with a cutting-edge nucleic acid amplification method known as loop-mediated isothermal amplification to detect bacterial pathogens precisely and efficiently. The air sampling system comprises a specialized collection device capable of capturing airborne bacteria particles, and an automatic sample extraction system from this device. Subsequently, nucleic acid amplification is performed using the LAMP technique, which can amplify at a constant temperature (65 °C) without the need for thermal cycling as with polymerase chain reaction, providing a faster, less costly, isothermal, and highly sensitive method for detecting target bacterial DNA. In addition, a simple, easily portable, low-cost, high-efficiency heating device that can be operated at constant temperature for the desired time has been developed for the LAMP reactions. Results: The results demonstrate the capability of the system to detect a broad-spectrum bacteria concentration with high sensitivity and specificity, making it a valuable tool for real-time monitoring and surveillance. The limit of detection of the air sampling system is 65 CFU L^-1, determined through a series of experiments. Conclusions: The proposed air sampling system, coupled with the LAMP technology, offers a promising solution for addressing the limitations associated with traditional bacterial detection methods in airborne environments, such as insufficient sensitivity, extended processing times, microbial drying, isolating target microorganisms from others, and challenges in detecting low concentrations, paving the way for enhanced public health and safety measures. Graphical

Robot teknolojisi sürekli olarak gelişmekte ve ülkemizde de bu alanda yapılan çalışmalar hızla artmaktadır. Üniversiteler, makine- imalat ve savunma sanayii firmaları sadece kullanıcı olmayıp, robot alanında halihazırda projeler yapmakta veya yapmayı planlamaktadır. Bu çalışma, çift kol olarak veya insan operatör ile birlikte çalışabilmesi için işbirlikçi olması istenen bir insansı robot kolu tasarımını ve kontrolünü kapsamaktadır. Proje kapsamında mekanik tasarım, dinamik analizler, ters kinematik çözüm, kinesitetik öğrenme, empedans kontrolü, yazılım ve donanım çalışmaları yapılmıştır. İnsansı robot kolunun üretilmesinden kontrolüne kadar olan aşamalar, karşılaşılan problemler, elde edilen tecrübeler ve gelişmiş tasarımlar için yapılan öneriler bu makalede kapsamlı bir şekilde sunulmuştur. Özellikle ticari amaçla geliştirilmekte olan yerli robot projelerine yararlı olması amacı ile kolay anlaşılır bir şekilde anlatılmıştır. Mekanik tasarım, dinamik analizler, benzetim, ve diğer dosyalar, ilgilenen araştırmacılarla açık kaynaklı olarak paylaşılacaktır.

Rapid and easy detection of pathogens, especially bacteria play an important role in daily life as we are facing increasing pollution. Trending research particularly focuses on nanotubes, which can bind different molecules to large surface areas and have high electron conductivity. They are widely employed in the development of electrochemical biosensors. This study presents a new design of an electrochemical biosensor integrated with gold tungsten wires and acid-functionalized multi-walled carbon nanotubes involving a two-step coating process Gold-plated tungsten wires are coated with acid-functionalized short multi-walled carbon nanotubes. FTIR and SEM analysis results present that immobilization of the electrodes is efficient. The detection performance of Escherichia coli K-12 is evaluated via the changes in electrical currents after antibody-antigen interactions. Bioimpedance measurements are performed at a certain frequency range. The measurements exhibit the electrode has a high detection capability for 0.8 CFU/mL. The response of the electrode saturates when the bacterial concentration is increased to 1.70x102 CFU/mL. The study reveals that the new biosensor is capable of detecting E. coli with a linear range between 8x10-1-1.70x102 CFU/mL. The data demonstrate that this multi-walled carbon nanotube-based biosensor can be developed into a high-sensitivity detection device for Escherichia coli.

Nosemosis, caused by the microsporidian parasites Nosema apis and Nosema ceranae, is a major contributor to honeybee (Apis mellifera) colony losses, yet its diagnosis still relies on destructive sampling followed by light microscopy or nucleic-acid amplification in a laboratory. We describe an impedimetric transducer intended as a first step toward a non-destructive alternative. Interdigitated gold electrodes were patterned on standard microscope slides by nanosecond laser ablation, a route that requires neither photolithography nor cleanroom processing, and were functionalized with (3-aminopropyl)triethoxysilane (APTES) deposited from either ethanol or toluene. Because the 0.01–50 MHz measurement window lies far above the range in which faradaic charge transfer contributes measurably, the response is non-faradaic and is governed by the permittivity and the ionic conductivity of the deposited droplet rather than by interfacial charge transfer. Films deposited from ethanol gave the largest and most reproducible contrast between an aqueous spore suspension (1500 spores/µL) and a deionized-water control. At the structural resonance of the electrode (≈16 MHz) the contrast in impedance magnitude is approximately 10% and is not resolved above the chip-to-chip scatter, which is expected because the magnitude at resonance is set by the series resistance of the electrode path rather than by the droplet. The two liquids are instead separated by the reactive component: the imaginary part of the impedance differs by 15.7 ± 1.8 MΩ near 6.8 MHz, about nine times the replicate uncertainty, and the phase by 33 ± 7° at 4.5 MHz. A self-contained readout built around an ESP32 microcontroller reproduced the direction of this change, its output voltage rising by approximately 33% after the suspension was applied. The scope of these observations is deliberately narrow: a single spore concentration in deionized water, no spore-free filtrate control, no calibration series, no selectivity panel and no measurement in a fecal matrix. The contribution is accordingly methodological—a cleanroom-free fabrication route, a quantified solvent dependence of the APTES film, and the transfer of the measurement from a benchtop spectrometer to a portable unit—rather than a validated diagnostic assay.