Background: Heat stress is a major restrictive factor that causes yield loss in rice. We previously reported the priming effect of abscisic acid (ABA) on rice for enhanced thermotolerance at the germination, seedling and heading stages. In the present study, we aimed to understand the priming effect and mechanism of ABA on grain filling capacity in rice under heat stress. Results: Rice plants were pretreated with distilled water, 50 μM ABA and 10 μM fluridone by leaf spraying at 8 d or 15 d after initial heading (AIH) stage and then were subjected to heat stress conditions of 38 °C day/30 °C night for 7 days, respectively. Exogenous ABA pretreatment significantly super-activated the ABA signaling pathway and improved the SOD, POD, CAT and APX enzyme activity levels, as well as upregulated the ROS-scavenging genes; and decreased the heat stress-induced ROS content (O₂^– and H₂O₂) by 15.0–25.5% in rice grain under heat stress. ABA pretreatment also increased starch synthetase activities in rice grain under heat stress. Furthermore, ABA pretreatment significantly improved yield component indices and grain yield by 14.4–16.5% under heat stress. ABA pretreatment improved the milling quality and the quality of appearance and decreased the incidence of chalky kernels and chalkiness in rice grain and improved the rice grain cooking quality by improving starch content and gel consistence and decreasing the amylose percentage under heat stress. The application of paraquat caused overaccumulation of ROS, decreased starch synthetase activities and ultimately decreased starch content and grain yield. Exogenous antioxidants decreased ROS overaccumulation and increased starch content and grain yield under heat stress. Conclusion: Taken together, these results suggest that exogenous ABA has a potential priming effect for enhancing rice grain filling capacity under heat stress at grain filling stage mainly by inhibiting ROS overaccumulation and improving starch synthetase activities in rice grain.

While enhanced phytoplankton growth as a result of iron (Fe) fertilization has been extensively characterized, our understanding of the underlying mechanisms remains incomplete. Here, we show in a laboratory setup mimicking Fe fertilization in the field that transcriptome remodeling is a primary driver of acclimation to Fe availability in the marine diazotrophic cyanobacterium Trichodesmium erythraeum IMS101. Fe supplementation promoted cell growth, photosynthesis and N ₂ fixation, and concomitant expression of the photosynthesis and N ₂ fixation genes. The expression of genes encoding major Fe-binding metalloproteins is tightly linked to cellular carbon and nitrogen metabolism and appears to be controlled by the ferric uptake regulator FurA, which is involved in regulating Fe uptake and homeostasis. This feedback loop is reinforced by substitutive expression of functionally equivalent or competitive genes depending on Fe availability, as well as co-expression of multiple Fe stress inducible isiA genes, an adaptive strategy evolved to elicit the Fe-responsive cascade. The study provides a genome-wide perspective on the acclimation of a prominent marine diazotroph to Fe availability, reveals an upgraded portfolio of indicator genes that can be used to better assess Fe status in the environment, and predicts scenarios of how marine diazotrophs may be affected in the future ocean. IMPORTANCE: The scarcity of trace metal iron (Fe) in global oceans has a great impact on phytoplankton growth. While enhanced primary productivity as a result of Fe fertilization has been extensively characterized, the underlying molecular mechanisms remain poorly understood. By subjecting the model marine diazotroph Trichodesmium erythraeum IMS101 to increasing concentrations of supplemented Fe, we demonstrate in it a comprehensively remodeled transcriptome that drives the mobilization of cellular Fe for coordinated carbon and nitrogen metabolism and reallocation of energy and resources. Our data provide broad genomic insight into marine diazotrophs acclimation to Fe availability, enabling the versatility and flexibility in choice of indicator genes for monitoring Fe status in the environment and having implications on how marine diazotrophs persist into the future ocean.

Photosynthetic carbon assimilation under photorespiratory conditions requires tight coordination between chloroplast electron transport and mitochondrial redox metabolism, yet the contribution of mitochondrial alternative oxidase (AOX) remains unresolved. Using 1 mM salicylhydroxamic acid (SHAM) to inhibit the AOX pathway in Rumex K-1 leaves, we investigated how mitochondrial alternative respiration contributes to carbon assimilation. AOX inhibition imposed a non-stomatal limitation on CO₂ assimilation and reduced photosystem II (PSII) electron transport. However, low O₂ or elevated CO₂ alleviated the decline in CO₂ assimilation while PSII photochemistry remained depressed, and AOX inhibition reduced apparent V_cmax without significantly affecting J_max, indicating that the primary constraint lay downstream of PSII in photorespiratory carbon metabolism. Electron flux through PSII and the electron fluxes supporting the photosynthetic carbon reduction and photorespiratory carbon oxidation cycles all decreased under SHAM treatment, indicating reduced PSII electron transport and electron use associated with carbon assimilation and photorespiration. AOX inhibition caused glycine accumulation and increased the Gly/Ser ratio under illumination but not in darkness, indicating restricted mitochondrial glycine-to-serine conversion during photorespiration. Together, these responses suggest that AOX-dependent ubiquinol oxidation helps sustain mitochondrial NADH reoxidation and NAD⁺ regeneration, thereby supporting glycine-to-serine conversion and photorespiratory carbon recycling.

Rice is central to global food security, and plant factories enable precise control of lighting conditions. However, genotype-specific responses to supplemental blue light across the full crop cycle remain poorly understood. Here, Nipponbare and CP511 were grown from transplanting to maturity under a green, red, and far-red background supplemented with 0, 50, 100, 150, or 300 μmol m⁻² s⁻¹ blue light (B0–B300). In both genotypes, increasing supplementation reduced plant height, increased tillering and biomass, advanced flowering by 9–13 d, and enhanced pigment content and photosynthetic capacity. Nipponbare maintained photosynthetic improvements at higher supplementation levels, whereas CP511 plateaued earlier. In Nipponbare, grain yield increased from 1.46 g plant⁻¹ at B0 to 5.09 g plant⁻¹ at B100 but did not increase significantly thereafter, despite enhanced photosynthesis and sucrose metabolism. At B300, seed setting declined to 54%, with reduced dry-matter allocation to panicles. In CP511, higher supplementation increased total panicle production but not effective panicle formation, seed setting, or grain yield, which changed from 3.29 g plant⁻¹ at B150 to 2.49 g plant⁻¹ at B300. Thus, the most favorable treatment depended on coordination between source capacity and effective reproductive sink formation rather than maximal photosynthesis or panicle production alone.