The composition and distribution of wetland vegetation is critical for ecosystem diversity and sustainable development. However, tidal flat wetland environments are complex, and obtaining effective satellite imagery is challenging due to the high cloud coverage. Moreover, it is difficult to acquire phenological feature data and extract species-level wetland vegetation information by using only spectral data or individual images. To solve these limitations, statistical features, temporal features, and phenological features of multiple Landsat 8 time-series images obtained via the Google Earth Engine (GEE) platform were compared to extract species-level wetland vegetation information from Chongming Island, China. The results indicated that (1) a harmonic model obtained the phenological characteristics of wetland vegetation better than the raw vegetation index (VI) and the Savitzky–Golay (SG) smoothing method; (2) classification based on the combination of the three features provided the highest overall accuracy (85.54%), and the phenological features (represented by the amplitude and phase of the harmonic model) had the greatest impact on the classification; and (3) the classification result from the senescence period was more accurate than that from the green period, but the annual mapping result on all seasons was the most accurate. The method described in this study can be applied to overcome the impacts of the complex environment in tidal flat wetlands and to effectively classify wetland vegetation species using GEE. This study could be used as a reference for the analysis of the phenological features of other areas or vegetation types.

A system understanding of the patterns, causes, and trends of long-term land use and land cover (LULC) change at the regional scale is essential for policy makers to address the growing challenges of local sustainability and global climate change. However, it still remains a challenge for estuarine and coastal regions due to the lack of appropriate approaches to consistently generate accurate and long-term LULC maps. In this work, an object-based classification framework was designed to mapping annual LULC changes in the Yangtze River estuary region from 1985–2016 using Landsat time series data. Characteristics of the inter-annual changes of LULC was then analyzed. The results showed that the object-based classification framework could accurately produce annual time series of LULC maps with overall accuracies over 86% for all single-year classifications. Results also indicated that the annual LULC maps enabled the clear depiction of the long-term variability of LULC and could be used to monitor the gradual changes that would not be observed using bi-temporal or sparse time series maps. Specifically, the impervious area rapidly increased from 6.42% to 22.55% of the total land area from 1985 to 2016, whereas the cropland area dramatically decreased from 80.61% to 55.44%. In contrast to the area of forest and grassland, which almost tripled, the area of inland water remained consistent from 1985 to 2008 and slightly increased from 2008 to 2016. However, the area of coastal marshes and barren tidal flats varied with large fluctuations.

Dark carbon fixation (DCF), conducted mainly by chemoautotrophs, contributes greatly to primary production and the global carbon budget. Understanding the response of DCF process to climate warming in coastal wetlands is of great significance for model optimization and climate change prediction. Here, based on a 4-yr field warming experiment (average annual temperature increase of 1.5°C), DCF rates were observed to be significantly inhibited by warming in coastal wetlands (average annual DCF decline of 21.6%, and estimated annual loss of 0.08–1.5 Tg C yr⁻¹ in global coastal marshes), thus causing a positive climate feedback. Under climate warming, chemoautotrophic microbial abundance and biodiversity, which were jointly affected by environmental changes such as soil organic carbon and water content, were recognized as significant drivers directly affecting DCF rates. Metagenomic analysis further revealed that climate warming may alter the pattern of DCF carbon sequestration pathways in coastal wetlands, increasing the relative importance of the 3-hydroxypropionate/4-hydroxybutyrate cycle, whereas the relative importance of the dominant chemoautotrophic carbon fixation pathways (Calvin–Benson–Bassham cycle and W-L pathway) may decrease due to warming stress. Collectively, our work uncovers the feedback mechanism of microbially mediated DCF to climate warming in coastal wetlands, and emphasizes a decrease in carbon sequestration through DCF activities in this globally important ecosystem under a warming climate.

Quantifying the vegetation aboveground biomass (AGB) is crucial for evaluating environment quality and estimating blue carbon in coastal wetlands. In this study, a UAV-LiDAR was first employed to quantify the canopy height model (CHM) of coastal Phragmites australis (common reed). Statistical correlations were explored between two multispectral remote sensing data (Sentinel-2 and JL-1) and reed biophysical parameters (CHM, density, and AGB) estimated from UAV-LiDAR data. Consequently, the reed AGB was separately estimated and mapped with UAV-LiDAR, Sentinel-2, and JL-1 data through the allometric equations (AEs). Results show that UAV-LiDAR-derived CHM at pixel size of 4 m agrees well with the observed stem height (R² = 0.69). Reed height positively correlates with the basal diameter and negatively correlates with plant density. The optimal AGB inversion model was derived from Sentinel-2 data and JL-1 data with R² = 0.58, RMSE = 216.86 g/m² and R² = 0.50, RMSE = 244.96 g/m², respectively. This study illustrated that the synergy of UAV-LiDAR data and multispectral remote sensing images has great potential in coastal reed monitoring.

Urban forests are highly heterogeneous; information about the combined effect of forest classification scale and algorithm selection on the estimation accuracy for urban forests remains unclear. In this study, we chose Chongming eco-island in the mega-city of Shanghai, a national experimental carbon neutral construction plot in China, as the study object. Remote sensing estimation models (simple regression models vs. machine learning models) of forest carbon density were constructed across different classification scales (all forests, different forest types, and dominant tree species) based on high-resolution aerial photographs and Sentinel-2A remote sensing images, and a large number of field surveys and optimal models were screened by ten-fold cross-validation. The results showed that (1) in early 2020, the total forest area and carbon storage of Chongming eco-island were 307.8 km² and 573,123.6 t, respectively, among which the areal ratios and total carbon storage ratios of evergreen broad-leaved forest, deciduous broad-leaved forest, and warm coniferous forest were 51.4% and 53.3%, 33.5% and 32.8%, and 15.1% and 13.9%, respectively. (2) The average forest carbon density of Chongming eco-island was 18.6 t/ha, among which no differences were detected among the three forest types (i.e., 17.2–19.2 t/ha), opposite to what was observed among the dominant tree species (i.e., 14.6–23.7 t/ha). (3) Compared to simple regression models, machine learning models showed an improvement in accuracy performance across all three classification scales, with average rRMSE and rBias values decreasing by 29.4% and 53.1%, respectively; compared to the all-forests classification scale, the average rRMSE and rBias across the algorithms decreased by 25.0% and 45.2% at the forest-type classification scale and by 28.6% and 44.3% at the tree species classification scale, respectively. We concluded that refining the forest classification, combined with advanced prediction procedures, could improve the accuracy of carbon storage estimates for urban forests.