Tropical climates present environmental challenges to the stability of total mixed ration (TMR) in the feed bunk throughout the day. Increasing feeding frequency is a typical suggested strategy to compensate for TMR deterioration and to prevent refusal by animals. However, treatment with a propionic acid-based additive could stabilize the TMR enough to allow a reduction in daily feeding frequency. In the preliminary laboratory trial, the additive improved the aerobic stability of TMR, and the optimal dose of 2 mL/kg of fresh matter (FM) was selected for the Additive strategy in the subsequent animal performance trial. Twelve Holstein cows (mean ± standard deviation; 612 ± 158 kg of body weight, 27 ± 6.5 kg/d milk yield, 178 ± 60 days in milk, and 3.0 ± 0.5 of body condition score) were assigned to a switch-back design to evaluate the effect of two feeding management strategies: Control, with two daily feeding of a control TMR; and Additive, with cows receiving a single daily feeding of a TMR treated with the additive at 2 L/t FM. The TMR was composed of 446 g/kg of dry matter (DM) corn silage, 64.0 g/kg DM Tifton haylage, 211 g/kg DM rehydrated corn grain silage, 163 g/kg DM soybean meal, 92.5 g/kg DM citrus pulp, and 23.2 g/kg DM mineral-vitamin mix. Cows fed the Additive strategy significantly increased daily milk yield by 10.2% without changes in DM intake, although no difference was observed for the feed efficiency. Milk contents of total solids and fat were decreased for cows in the Additive strategy. An increase in somatic cell count was observed for cows in the Additive, which could be related to the faster first meal activity after milking than the cows in the Control strategy, rendering them more susceptible to teat contamination. Milk energy and milk urea nitrogen contents were not affected by treatments, although energy excretion through milk was greater for the cows in the Additive. The Additive strategy promoted a shift in the sorting behavior in favor of longer particles, increasing the time spent on rumination, and decreased the number of daily meals at the expense of larger meal size. Two contemporary trials were conducted to measure changes in the thermal and hygienic behavior of the experimental TMR offered in the animal performance trial, simulating aerobic exposure for a 24-h period in exclusive feed bunks installed in the free stall barn, without animal access. The Control TMR presented shorter aerobic stability for the middle and top layers in the feed bunk compared to the bottom layer, while the Additive TMR presented no differences across layers, suggesting a generalized microbial activity suppression independent of the protective effect of upper layers. The Additive TMR suppressed yeasts survival persistence and their activity. Our results suggest that treating TMR with the additive improves the maintenance of its hygienic and thermal profile in the feed bunk, which enables the strategy of a single daily feeding with significant benefits in animal performance.