Tetratricopeptide repeat (TPR) proteins play numerous roles in plant growth and development by mediating protein–protein interactions in biological systems by binding to peptide ligands. Although genome-wide analyses of the TPR gene family in other species have been performed, its evolution and function in Cucurbitaceae remain unclear. In this study, 144 TPR genes from 11 genomes of eight Cucurbitaceae species with a heterogeneous distribution on the chromosomes were characterized. Based on the homology between Cucurbitaceae and Arabidopsis, the TPR genes were divided into four groups, and the evolutionary relationships of the Benincaceae and Cucurbitaceae tribes were also represented in a phylogenetic tree. Using the ‘DHL92′ genome as a reference, an integrated chromosome map was obtained containing 34 loci, 4 of which were common to the Cucurbitaceae. Cis-regulatory element analysis showed that these elements are essential for melon development and responses to light, phytohormones, and various stresses. CmTPR tissue- and development-specific expression analysis revealed differential expression patterns under normal growth conditions. Furthermore, the CmTPR genes responded to various abiotic stressors. Overall, this study offers insights into the evolutionary history of the TPR gene family in Cucurbitaceae and provides valuable information for elucidating the potential role of CmTPR genes during development and under different stresses in melon.

The BARLEY B RECOMBINANT/BASIC PENTACYSTEINE (BBR/BPC) transcription factors are widely involved in plant development and abiotic stress responses. In this study, ten GmBPC genes were identified in the soybean genome, and their characteristics and expression patterns were comprehensively analyzed. Based on phylogenetic relationships, the GmBPCs were classified into two groups, and members within the same group exhibited high similarity in motif compositions and gene structures. Expression profiling indicated that GmBPCs were expressed in all tissues, with most showing high transcript levels in seeds. The GmBPCs exhibited different expression profiles in soybean under drought and heat stresses. Ectopic expression of GmBPC8 in Arabidopsis led to abnormal floral development and reduced the seed setting rate. Additionally, GmBPC8 could form both homodimers and heterodimers with other GmBPC proteins. Collectively, these findings provide a foundation for elucidating the functional roles of BPC transcription factors.