Obtaining sterile anticoagulated whole-blood units is a key prerequisite for downstream laboratory and clinical applications involving blood-derived products. In bovine medicine, however, published information on blood collection into bags is mainly embedded in transfusion-oriented references or broader downstream studies, while formal validation of donor-oriented collection methods remains limited. The aim of this study was to develop and field-validate a large-volume bag-based method for whole-blood collection in donor dairy cows under commercial farm conditions. Whole-blood collections were performed in clinically healthy donor dairy cows using a jugular venipuncture protocol that was progressively refined during field validation. A true sequential multi-bag setup, enabled by extension tubing and a perforable connection device, was introduced on 24 October 2022. Protocol validation was based primarily on procedural success, defined as a bacteriologically negative blood unit with a collected volume of at least 400 mL per bag. A total of 294 procedures were included. Overall, 228/294 procedures (77.6%) were classified as successful, 48/294 (16.3%) as suboptimal, and 18/294 (6.1%) as failed. The median collected volume per procedure was 410 mL (IQR 400–430; range 124.5–1,280 mL). Overall, 276/294 collections (93.9%) were bacteriologically negative. Within the single-bag phase, later collection dates were associated with a lower probability of bacteriological positivity, supporting a learning-curve effect on microbiological reliability. Later implementation of the sequential multi-bag setup was associated with a higher total volume per procedure, without an apparent loss of microbiological safety. In conclusion, this large-volume bag-based protocol proved feasible for whole-blood collection in donor dairy cows under field conditions and may provide a practical upstream basis for subsequent laboratory applications and downstream processing workflows.

Current agricultural systems are expected to balance production efficiency with a conscious management of natural resources, to meet evolving consumer expectations at both environmental and ethical...

Platelet-based blood products, including platelet-rich plasma, platelet concentrate, and platelet lysate, are increasingly investigated in veterinary medicine as biological preparations with proposed regenerative, anti-inflammatory, and immunomodulatory properties. In bovine and equine medicine, their use has expanded across several clinical fields, but blood collection and product preparation methods have not been systematically synthesized. This systematic scoping review aimed to describe the literature on platelet-based blood products in bovine and equine medicine, summarize methodological characteristics of blood collection and preparation methods, and analyze studies addressing bovine mammary disorders. Searches were performed in Scopus, Web of Science Core Collection, CAB Abstracts, and PubMed on 25 August 2026, with no publication-year or start-date restriction. Eligible studies were original peer-reviewed articles in English; fibrin- or gel-based preparations were excluded when the corresponding liquid platelet-based product could not be separately characterized or evaluated, as were scaffold or combined constructs when the platelet-based component could not be evaluated separately. No formal review protocol was registered or published. Studies providing eligible bovine and/or equine data and reporting at least one methodological aspect related to collection, preparation, activation or lysis, storage, characterization, or administration were eligible. A total of 191 studies were included. Studies contributing equine data only among the target species predominated (161/191; 84.3%), whereas 29 studies (15.2%) contributed bovine data only and one study (0.5%) included both bovine and equine data. The PRP family, including PRP and related PRP-type preparations, was the most frequently investigated product group (134/191; 70.2%), followed by platelet lysate (46/191; 24.1%) and platelet concentrate (19/191; 9.9%); categories were not mutually exclusive. Methods varied in donor source, collection site, blood volume, anticoagulant, collection system, centrifugation workflow, activation or lysis, storage, and characterization. The main blood collection and preparation workflow was reconstructable in 111 studies (58.1%) and partially reconstructable in 80 studies (41.9%). Methodological reporting was frequently incomplete, particularly for leukocyte concentration, activation or lysis procedures, storage conditions, and product-level sterility or microbiological quality control. Product-level sterility or microbiological quality control was explicitly reported in 7 studies (3.7%) and partially reported in one study (0.5%). Only five studies addressed bovine mammary disorders and investigated methodologically distinct products. More complete and consistent methodological reporting is needed to improve reproducibility and comparability across studies.