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Dr. Lixin Wen
Hunan Agriculture University

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0 Lipid Metabolism
0 Nutrition
0 Obesity
0 NAFLD
0 Dietary pattern

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Journal article
Published: 09 June 2021 in Environmental Pollution
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Zearalenone (ZEA) is an estrogenic toxin produced by Fusarium strains, that is widely present in crops, and endangers the reproductive system of animals. Tannic acid (TA) is a natural polyphenolic substance that is widespread in the roots, stems, and leaves of plants, and has special pharmacological activity. This study was designed to investigate the therapeutic effect of TA on ZEA-induced ovarian damage in mice and to explore the molecular mechanism involved. Ninety healthy Kunming female mice were divided into six equal groups. All the groups but the control group were administered daily with ZEA [10 mg/kg body weight (bw)] orally, for 7 days, to induce damage to the reproductive system. Some groups were also administered with TA (50, 100, and 200 mg/bw) for 7 days. Mice were euthanized 24 h later to allow for collection of serum and ovaries. TA can effectively alleviate the appearance of congestion and redness of the ovary, caused by ZEA, and increase the number of healthy growing follicles. Moreover, the estrogen content and the levels of MDA and ROS in the ovaries can be effectively reduced by TA. It can also reduce the apoptosis of ovarian cells, decreases the protein expression of the estrogen receptor, Fas, Fasl, caspase-3, caspase-8, caspase-9, and Bax, and increases the protein expression of Bcl-2. Our study indicates that TA reduces the strong estrogen and oxidative damage induced by ZEA, and these therapeutic effects may be partially mediated by the death receptor and mitochondrial apoptosis signaling pathway.

ACS Style

Jing Wu; Jiayan Li; Yanwei Liu; Xinxin Liao; Dongyi Wu; Yunqin Chen; Zengenni Liang; Zhihang Yuan; Rongfang Li; Jine Yi; Lixin Wen. Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice. Environmental Pollution 2021, 287, 117557 .

AMA Style

Jing Wu, Jiayan Li, Yanwei Liu, Xinxin Liao, Dongyi Wu, Yunqin Chen, Zengenni Liang, Zhihang Yuan, Rongfang Li, Jine Yi, Lixin Wen. Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice. Environmental Pollution. 2021; 287 ():117557.

Chicago/Turabian Style

Jing Wu; Jiayan Li; Yanwei Liu; Xinxin Liao; Dongyi Wu; Yunqin Chen; Zengenni Liang; Zhihang Yuan; Rongfang Li; Jine Yi; Lixin Wen. 2021. "Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice." Environmental Pollution 287, no. : 117557.

Commentary
Published: 05 November 2020 in Food Science & Nutrition
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ACS Style

Sisi Yan; Xiangyan Liu; Xin Li; Xiaowen Li; Ji Wang; Lixin Wen. Critique of the Chinese dietary guidelines on the consumption of cooking oils. Food Science & Nutrition 2020, 9, 583 -585.

AMA Style

Sisi Yan, Xiangyan Liu, Xin Li, Xiaowen Li, Ji Wang, Lixin Wen. Critique of the Chinese dietary guidelines on the consumption of cooking oils. Food Science & Nutrition. 2020; 9 (1):583-585.

Chicago/Turabian Style

Sisi Yan; Xiangyan Liu; Xin Li; Xiaowen Li; Ji Wang; Lixin Wen. 2020. "Critique of the Chinese dietary guidelines on the consumption of cooking oils." Food Science & Nutrition 9, no. 1: 583-585.

Journal article
Published: 16 October 2020 in Research in Veterinary Science
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Newcastle Disease Virus (NDV) is the important pathogen of Newcastle Disease (ND) attacking chicken, turkey and other birds. Therefore, the purpose of this study was to assess the effects of immune stress induced by ND vaccination and lipopolysaccharide (LPS) on growth performance, antioxidant ability, and lipid metabolism of broilers. In total, 128 one-day-old broilers were randomly assigned to the following four groups and were treated as indicated: normal control (NC); vaccinated with live LaSota ND vaccine (CV); administered ND vaccine and 0.25 mg/kg body weight (BW) LPS (L-LPS); and administered ND vaccine and 0.5 mg/kg BW LPS (H-LPS). The results demonstrated that broiler feed conversion ratio (FCR) was increased in the groups CV, L-LPS and H-LPS from d 0 to 42 days compared with the group NC. The antioxidant function of broilers was decreased as indicated by the malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels in the serum of the treated groups. ND vaccination combined LPS increased the concentration of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), but decreased the concentration of high-density lipoprotein cholesterol (HDLC) compared with the group NC. The reverse transcription (RT)-PCR results revealed that the mRNA expression of acetyl-CoA carboxylase gene (ACC) and 3-hydroxy-3-methylglutaryl coenzyme-A reductase (HMGR) in the liver were downregulated, whereas the mRNA expression of carnitine palmitoyltransferase-1 (CPT-1) and peroxisome proliferator-activated receptor (PPAR)-α were upregulated compared with the group NC. These results suggest that ND vaccination combined LPS reduced broiler growth performance and antioxidant ability, whereas it activated AMPK-mediated lipid metabolism.

ACS Style

Xiaowen Li; Shuiping Liu; Ji Wang; Jine Yi; Zhihang Yuan; Jing Wu; Lixin Wen; Rongfang Li. Effects of ND vaccination combined LPS on growth performance, antioxidant performance and lipid metabolism of broiler. Research in Veterinary Science 2020, 1 .

AMA Style

Xiaowen Li, Shuiping Liu, Ji Wang, Jine Yi, Zhihang Yuan, Jing Wu, Lixin Wen, Rongfang Li. Effects of ND vaccination combined LPS on growth performance, antioxidant performance and lipid metabolism of broiler. Research in Veterinary Science. 2020; ():1.

Chicago/Turabian Style

Xiaowen Li; Shuiping Liu; Ji Wang; Jine Yi; Zhihang Yuan; Jing Wu; Lixin Wen; Rongfang Li. 2020. "Effects of ND vaccination combined LPS on growth performance, antioxidant performance and lipid metabolism of broiler." Research in Veterinary Science , no. : 1.

Research article
Published: 30 June 2020 in Oil Crop Science
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Lard, a fat rich in saturated fatty acids (SFAs), is regarded as a risk factor for metabolic diseases. Our previous study found that lard blended with soybean oil has an anti-obesity effect in adult mice. In the present study, effect of different lard blended with sunflower oil diet on lipid accumulation in adipose tissue, liver, and serum by mouse model was researched. Body weight, body fat percentage, cross-sectional area of adipocytes, liver triglycerides (TGs), and oil red stained area in mice liver of lard blend sunflower oil (L-SFO) group were significantly lower than those of sunflower oil (SFO) group, whereas no significant differences were observed between mice of lard and L-SFO groups. Serum TG and free fatty acid (FFA) levels were significantly lower in L-SFO group than in other two groups. Furthermore, data showed that sunflower oil decreased contents of hormone-sensitive lipase and carnitine palmitoyl transferase 1 (CPT-1) and increased fatty acid synthase activity in liver tissue. A mixture of lard and sunflower oil rather than only sunflower oil or lard might promote body fat loss and reduce lipid accumulation in adipose tissue, serum, and liver by promoting hydrolysis of TG, increasing β-oxidation of fatty acids. These data suggested that the use of a mixture of lard and vegetable oil (e.g. sunflower oil) for cooking, or the alternate use of lard and vegetable, may be beneficial for reducing body fat.

ACS Style

Sisi Yan; Xin Li; Linyu Zhang; Yu Zeng; Shuiping Liu; Xiangyan Liu; Huijuan Zhou; Lixin Wen; Ji Wang. Moderate quantity of lard mixed with sunflower oil attenuate lipid accumulation in mice. Oil Crop Science 2020, 5, 205 -212.

AMA Style

Sisi Yan, Xin Li, Linyu Zhang, Yu Zeng, Shuiping Liu, Xiangyan Liu, Huijuan Zhou, Lixin Wen, Ji Wang. Moderate quantity of lard mixed with sunflower oil attenuate lipid accumulation in mice. Oil Crop Science. 2020; 5 (4):205-212.

Chicago/Turabian Style

Sisi Yan; Xin Li; Linyu Zhang; Yu Zeng; Shuiping Liu; Xiangyan Liu; Huijuan Zhou; Lixin Wen; Ji Wang. 2020. "Moderate quantity of lard mixed with sunflower oil attenuate lipid accumulation in mice." Oil Crop Science 5, no. 4: 205-212.

Journal article
Published: 10 March 2020 in Poultry Science
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This study was conducted to assess the growth performance and immunological effects of vaccination-induced stress on broilers. The chickens were administered 0, 2, 4, 8, and 16 doses of live LaSota Newcastle disease (ND) vaccine and slaughtered on the 1st, 7th, 14th, and 21st day post vaccination. The results showed that the serum antibody titers after Newcastle disease virus (NDV) vaccination were elevated at day 7 post vaccination, peaked at day 14, then declined by day 21. Interestingly, the antibody titers peaked at 2 doses, and no further dose-dependent titer increases were observed. This study demonstrated that vaccination-induced stress increased serum adrenocorticotropic hormone and cortisol, affected growth performance (average daily gain, average daily feed intake, and feed conversion ratio), and triggered apoptosis in spleen lymphocytes by downregulating the ratio of Bcl-2 to BAX and upregulating the gene expressions of caspase-3 and -9, which was concordant with the activation of the enzymatic activities of caspase-3 and -9. This study suggests that NDV vaccine doses in broilers must be controlled judiciously because increasing the number of doses resulted in increased lymphocyte apoptosis while the peak of the antibody titer and optimal growth performance were achieved at a low number of doses (2 doses).

ACS Style

R.F. Li; S.P. Liu; Z.H. Yuan; J.E. Yi; Y.N. Tian; J. Wu; L.X. Wen. Effects of induced stress from the live LaSota Newcastle disease vaccination on the growth performance and immune function in broiler chickens. Poultry Science 2020, 99, 1896 -1905.

AMA Style

R.F. Li, S.P. Liu, Z.H. Yuan, J.E. Yi, Y.N. Tian, J. Wu, L.X. Wen. Effects of induced stress from the live LaSota Newcastle disease vaccination on the growth performance and immune function in broiler chickens. Poultry Science. 2020; 99 (4):1896-1905.

Chicago/Turabian Style

R.F. Li; S.P. Liu; Z.H. Yuan; J.E. Yi; Y.N. Tian; J. Wu; L.X. Wen. 2020. "Effects of induced stress from the live LaSota Newcastle disease vaccination on the growth performance and immune function in broiler chickens." Poultry Science 99, no. 4: 1896-1905.

Journal article
Published: 29 February 2020 in Lipids in Health and Disease
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Background The traditional Chinese diet blends lard with vegetable oil, keeping the fatty acid balance intake ratio of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids at nearly 1:1:1. However, the effects of a mixture of lard and vegetable oil on lipid metabolism have never been researched. In the present study, by simulating Chinese high-fat dietary habits, we explored the effects of a mixture of lard and vegetable oil on lipid metabolism. Methods We randomly assigned 50 male C57BL/6 J mice to 5 groups (10 in each group) and fed them lard, sunflower oil (SFO), soybean oil (SBO), lard blended with sunflower oil (L-SFO), or lard blended with soybean oil (L-SBO) for 12 weeks. Results We found that the final body weights of mice in the lard group were significantly higher than those of mice in the SFO and SBO groups. Body fat rate and volume of fat cell of the lard group were significantly higher than those of the SFO, SBO, and L-SBO groups. Liver triglyceride level of the lard group increased significantly compared to the other groups. Although body fat rate and liver triglyceride level in the SBO and SFO groups decreased compared to those in the other groups, the high-density lipoprotein cholesterol/low-density lipoprotein cholesterol ratio were also significantly decreased in the SBO and SFO groups. Conclusions We found that a lard diet induced accumulation of body fat, liver and serum lipids, which can increase the risk of obesity, non-alcoholic fatty acid liver disease, and atherosclerosis. The vegetable oil diet resulted in cholesterol metabolism disorders even though it did not lead to obesity. The mixed oil diet induced body fat accumulation, but did not cause lipid accumulation in the liver and serum. Thus, differential oil/fat diets have an impact on differential aspects in mouse lipid metabolism. Graphical abstract

ACS Style

Sisi Yan; Huijuan Zhou; Shuiping Liu; Ji Wang; Yu Zeng; Froilan Bernard Matias; Lixin Wen. Differential effects of Chinese high-fat dietary habits on lipid metabolism: mechanisms and health implications. Lipids in Health and Disease 2020, 19, 1 -9.

AMA Style

Sisi Yan, Huijuan Zhou, Shuiping Liu, Ji Wang, Yu Zeng, Froilan Bernard Matias, Lixin Wen. Differential effects of Chinese high-fat dietary habits on lipid metabolism: mechanisms and health implications. Lipids in Health and Disease. 2020; 19 (1):1-9.

Chicago/Turabian Style

Sisi Yan; Huijuan Zhou; Shuiping Liu; Ji Wang; Yu Zeng; Froilan Bernard Matias; Lixin Wen. 2020. "Differential effects of Chinese high-fat dietary habits on lipid metabolism: mechanisms and health implications." Lipids in Health and Disease 19, no. 1: 1-9.

Journal article
Published: 26 November 2019 in Biomolecules
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T-2 toxin is one of the most toxic type A trichothecene mycotoxins in nature, and it exhibits reproductive toxicity. Betulinic acid (BA) is a natural pentacyclic triterpene compound found in species of Betula, and it has been reported to have antioxidant activity. The aim of the present study was to investigate the protective effect of BA on T-2-toxin-induced testicular injury in mice and explore its molecular mechanism. Sixty adult male mice were randomly divided into groups. The mice were pretreated orally with BA (0.25, 0.5, and 1.0 mg/kg) daily for 14 days, and the T-2 toxin (4 mg/kg body weight) was administered via intraperitoneal injection to induce oxidative stress after the last administration of BA. BA pretreatment significantly increased the secreted levels of testosterone and sperm motility. Moreover, BA pretreatment significantly increased the total antioxidant capacity (T-AOC), the activity of SOD and CAT, and the content of GSH, and it reduced the content of MDA. Furthermore, BA relieved testicular injury and reduced the number of apoptotic cells, and it significantly decreased the protein expression of Janus kinase 2 (JAK2), signal transducers and activators of transcription 3 (STAT3), caspsae-3, and Bcl-2-associated X protein (Bax). BA also increased the expression of B-cell lymphoma-2 (Bcl-2). We suggest that BA reduced the oxidative damage induced by T-2 toxin, and that these protective effects may be partially mediated by the JAK2/STAT3 signaling pathway.

ACS Style

Jing Wu; Chenglin Yang; Juan Liu; Jiaxin Chen; Chao Huang; Ji Wang; Zengenni Liang; Lixin Wen; Jin-E Yi; Zhihang Yuan. Betulinic Acid Attenuates T-2-Toxin-Induced Testis Oxidative Damage Through Regulation of the JAK2/STAT3 Signaling Pathway in Mice. Biomolecules 2019, 9, 787 .

AMA Style

Jing Wu, Chenglin Yang, Juan Liu, Jiaxin Chen, Chao Huang, Ji Wang, Zengenni Liang, Lixin Wen, Jin-E Yi, Zhihang Yuan. Betulinic Acid Attenuates T-2-Toxin-Induced Testis Oxidative Damage Through Regulation of the JAK2/STAT3 Signaling Pathway in Mice. Biomolecules. 2019; 9 (12):787.

Chicago/Turabian Style

Jing Wu; Chenglin Yang; Juan Liu; Jiaxin Chen; Chao Huang; Ji Wang; Zengenni Liang; Lixin Wen; Jin-E Yi; Zhihang Yuan. 2019. "Betulinic Acid Attenuates T-2-Toxin-Induced Testis Oxidative Damage Through Regulation of the JAK2/STAT3 Signaling Pathway in Mice." Biomolecules 9, no. 12: 787.

Journal article
Published: 03 September 2019 in Journal of Functional Foods
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Gamma-oryzanol (Orz), a mixture of ferulic acid esters of plant sterols and triterpene alcohols, has been proven to possess multiple activities. The purpose of this study was to explore the hepatoprotective effects and potential mechanisms of Orz against ethanol-induced liver injury in mice. Orz pretreatment significantly reduced the serum levels of ALP, ALT, AST and CYP2E1, and increased the TP and GLB levels in the ethanol-challenged mice. Orz increased the activities of CAT, SOD, GSH-PX and the GSH content in the liver, while decreased hepatic steatosis in ethanol-induced liver injure. Furthermore, Orz significantly decreased the protein expression of ASK1, JNK, P38, active-caspase-9 and active-caspase-3, and the protein ratio of Bax/Bcl-2 in livers of ethanol-challenged mice. These results suggest that Orz provides protection against ethanol-induced liver injury which might be due to its alleviation of oxidative stress and inhibition of apoptosis, possibly inhibiting MAPK signaling pathways mediated mitochondrial signaling pathway activation.

ACS Style

Lijuan Zhu; Siting Xiang; Zhaoping Ou; Chaoyang Ma; Xianglian Yi; Lin Huang; Yurong Ma; Zengenni Liang; Zhihang Yuan; Lixin Wen; Rongfang Li; Jing Wu; Zhuliang Tan; Jine Yi. Gamma-oryzanol prevents ethanol-induced liver injury by ameliorating oxidative stress and modulating apoptosis-related protein expression in mice. Journal of Functional Foods 2019, 62, 103532 .

AMA Style

Lijuan Zhu, Siting Xiang, Zhaoping Ou, Chaoyang Ma, Xianglian Yi, Lin Huang, Yurong Ma, Zengenni Liang, Zhihang Yuan, Lixin Wen, Rongfang Li, Jing Wu, Zhuliang Tan, Jine Yi. Gamma-oryzanol prevents ethanol-induced liver injury by ameliorating oxidative stress and modulating apoptosis-related protein expression in mice. Journal of Functional Foods. 2019; 62 ():103532.

Chicago/Turabian Style

Lijuan Zhu; Siting Xiang; Zhaoping Ou; Chaoyang Ma; Xianglian Yi; Lin Huang; Yurong Ma; Zengenni Liang; Zhihang Yuan; Lixin Wen; Rongfang Li; Jing Wu; Zhuliang Tan; Jine Yi. 2019. "Gamma-oryzanol prevents ethanol-induced liver injury by ameliorating oxidative stress and modulating apoptosis-related protein expression in mice." Journal of Functional Foods 62, no. : 103532.

Journal article
Published: 22 August 2019 in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
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Phenethyl isothiocyanate (PEITC) is one of the glucosinolates (GLs) present in cruciferous vegetables. Although there are many reports of livestock and poultry poisoning caused by plants containing GLs, the actual dosage that causes poisoning and the characteristics of GLs and their metabolites are unclear. Herein, we investigated the inhibitory effects of PEITC on IPEC-J2 cells and examined the mechanisms of PEITC-induced apoptosis via the mitochondrial pathway. Cell viability was determined by the MTT assay, and the levels of reactive oxygen species, mitochondrial membrane potential (∆Ψ), intracellular Ca2+ concentration, and cell apoptosis were detected by flow cytometry. IPEC-J2 cells were collected to assess the activities of superoxide dismutase, catalase, and glutathione peroxidase, as well as the contents of glutathione, malondialdehyde, H2O2, ATP, and lactate dehydrogenase, using biochemical methods. The levels of cytochrome c, Bax, Bcl-2, caspase-3, caspase-9, poly (ADP-ribose) polymerase (PARP)-1, p53, CDC25C, and cyclin A2 were analyzed by western blotting. We found that PEITC effectively inhibited the growth of IPEC-J2 cells, causing apoptosis. PEITC suppressed the level of mitochondrial membrane potential; released cytochrome c from the mitochondria to the cytoplasm; reduced ATP levels; inhibited Bcl-2 expression; increased Bax expression; and activated caspase-9, caspase-3, and PARP-1, leading to apoptosis. PEITC also induced G2/M and S phase arrest by affecting cell cycle-associated proteins such as p53, CDC25C, and cyclin A2. We conclude that PEITC causes oxidative stress, cell cycle arrest, and apoptosis in IPEC-J2 cells via a mitochondrial-dependent Bax/Bcl-2 pathway.

ACS Style

Shuiping Liu; Yuanyuan Zhu; Sisi Yan; Haisi Xiao; Jine Yi; Rongfang Li; Jing Wu; Lixin Wen. Phenethyl isothiocyanate induces IPEC-J2 cells cytotoxicity and apoptosis via S-G2/M phase arrest and mitochondria-mediated Bax/Bcl-2 pathway. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 2019, 226, 108574 .

AMA Style

Shuiping Liu, Yuanyuan Zhu, Sisi Yan, Haisi Xiao, Jine Yi, Rongfang Li, Jing Wu, Lixin Wen. Phenethyl isothiocyanate induces IPEC-J2 cells cytotoxicity and apoptosis via S-G2/M phase arrest and mitochondria-mediated Bax/Bcl-2 pathway. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019; 226 ():108574.

Chicago/Turabian Style

Shuiping Liu; Yuanyuan Zhu; Sisi Yan; Haisi Xiao; Jine Yi; Rongfang Li; Jing Wu; Lixin Wen. 2019. "Phenethyl isothiocyanate induces IPEC-J2 cells cytotoxicity and apoptosis via S-G2/M phase arrest and mitochondria-mediated Bax/Bcl-2 pathway." Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 226, no. : 108574.

Journal article
Published: 09 July 2019 in Toxins
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The presence of tannic acid (TA), which is widely distributed in plants, limits the utilization of non-grain feed. Illustrating the toxicity mechanism of TA in animals is important for preventing poisoning and for clinical development of TA. The aim of the present study was to evaluate the toxic effects and possible action mechanism of TA in porcine intestinal IPEC-J2 cells, as well as cell proliferation, apoptosis, and cell cycle. We investigated the toxic effects of TA in IPEC-J2 cells combining the analysis of TA-induced apoptotic responses and effect on the cell cycle. The results revealed that TA is highly toxic to IPEC-J2 cells. The stress-inducible factors reactive oxygen species, malondialdehyde, and 8-hydroxy-2'-deoxyguanosine were increased in response to TA. Furthermore, TA suppressed mitochondrial membrane potential, reduced adenosine triphosphate production, and adversely affected B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein, caspase-9, caspase-3, cytochrome c, cyclin A, cyclin-dependent kinases, ataxia-telangiectasia mutated, and P53 expression in a dose-dependent manner. We suggest that TA induces the mitochondrial pathway of apoptosis and S phase arrest in IPEC-J2 cells.

ACS Style

Ji Wang; Haisi Xiao; Yuanyuan Zhu; Shuiping Liu; Zhihang Yuan; Jing Wu; Lixin Wen. Tannic Acid Induces the Mitochondrial Pathway of Apoptosis and S Phase Arrest in Porcine Intestinal IPEC-J2 Cells. Toxins 2019, 11, 397 .

AMA Style

Ji Wang, Haisi Xiao, Yuanyuan Zhu, Shuiping Liu, Zhihang Yuan, Jing Wu, Lixin Wen. Tannic Acid Induces the Mitochondrial Pathway of Apoptosis and S Phase Arrest in Porcine Intestinal IPEC-J2 Cells. Toxins. 2019; 11 (7):397.

Chicago/Turabian Style

Ji Wang; Haisi Xiao; Yuanyuan Zhu; Shuiping Liu; Zhihang Yuan; Jing Wu; Lixin Wen. 2019. "Tannic Acid Induces the Mitochondrial Pathway of Apoptosis and S Phase Arrest in Porcine Intestinal IPEC-J2 Cells." Toxins 11, no. 7: 397.

Journal article
Published: 31 October 2017 in Scientific Reports
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Obesity, which is associated with dietary habits, has become a global social problem and causes many metabolic diseases. In China, both percentages of adult obesity and overweight are far lower compared to western countries. It was designed to increase the two levels of daily intake in human, namely 3.8% and 6.5%, which are recommendatory intake (25 g/d) and Chinese citizens’ practical intake (41.4 g/d), respectively. The mice were respectively fed with feeds added with soybean oil, lard or the oil blended by both for 12 weeks. In the mice fed with diet containing 3.8% of the three oils or 6.5% blended oil, their body weight, body fat rate, cross-sectional area of adipocytes, adipogenesis and lipogenesis in adipose were decreased, whereas hydrolysis of triglyserides in adipose was increased. This study demonstrated that the oil mixture containing lard and soybean oil had a remarkable anti-obesity effect. It suggests that the traditional Chinese dietary habits using oils blended with lard and soybean oil, might be one of the factors of lower percentages of overweight and obesity in China, and that the increasing of dietary oil intake and the changing of its component resulted in the increasing of obesity rate in China over the past decades.

ACS Style

Ji Wang; Sisi Yan; Haisi Xiao; Huijuan Zhou; Shuiping Liu; Yu Zeng; Biying Liu; Rongfang Li; Zhihang Yuan; Jing Wu; Jine Yi; Yarou Bao Sero Razack; Lixin Wen. Anti-obesity effect of a traditional Chinese dietary habit—blending lard with vegetable oil while cooking. Scientific Reports 2017, 7, 1 -10.

AMA Style

Ji Wang, Sisi Yan, Haisi Xiao, Huijuan Zhou, Shuiping Liu, Yu Zeng, Biying Liu, Rongfang Li, Zhihang Yuan, Jing Wu, Jine Yi, Yarou Bao Sero Razack, Lixin Wen. Anti-obesity effect of a traditional Chinese dietary habit—blending lard with vegetable oil while cooking. Scientific Reports. 2017; 7 (1):1-10.

Chicago/Turabian Style

Ji Wang; Sisi Yan; Haisi Xiao; Huijuan Zhou; Shuiping Liu; Yu Zeng; Biying Liu; Rongfang Li; Zhihang Yuan; Jing Wu; Jine Yi; Yarou Bao Sero Razack; Lixin Wen. 2017. "Anti-obesity effect of a traditional Chinese dietary habit—blending lard with vegetable oil while cooking." Scientific Reports 7, no. 1: 1-10.

Journal article
Published: 01 September 2013 in Environmental Toxicology and Pharmacology
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To investigate the reproductive toxicity and cytotoxicity of T-2 toxin, which is a mycotoxin, and to explore its potential apoptotic induction mechanism. ovarian granulosa cells of rats were treated with T-2 toxin (1-100nM) for 24h. The cytotoxicity was assessed with MTT bioassay and apoptotic cells were detected by flow cytometry, and further identified by chromatin condensation and nuclear fragmentation with Hoechst 33258 under microscope; reactive oxygen species (ROS) with DCFH-DA was analyzed by fluorometry; total superoxide dismutase (SOD) was determined by NBT staining method. Glutathione peroxidase (GSHPx) activity was detected by UV colorimetric assay. The activity of catalase (CAT) in granulosa cells was measured by the Aebi method, and malondialdehyde (MDA) content was determined by thiobarbituric acid assay. T-2 toxin dose-dependently inhibited the growth of granulosa cells and resulted in apoptosis in rat granulosa cells. Treatment with T-2 toxin could induce ROS and MDA accumulation in granulosa cells, acompanying with losses of activities of SOD, GSH-Px and CAT, whereas T-2 toxin-induced apoptosis in granulosa cells could be significantly inhibited through the use of antioxidant Trolox. These data suggest that T-2 toxin may induce the apoptosis in rat granulosa cells through oxidative stress.

ACS Style

Jing Wu; Di Tu; Li-Yun Yuan; Hui Yuan; Li-Xin Wen. T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative stress. Environmental Toxicology and Pharmacology 2013, 36, 493 -500.

AMA Style

Jing Wu, Di Tu, Li-Yun Yuan, Hui Yuan, Li-Xin Wen. T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative stress. Environmental Toxicology and Pharmacology. 2013; 36 (2):493-500.

Chicago/Turabian Style

Jing Wu; Di Tu; Li-Yun Yuan; Hui Yuan; Li-Xin Wen. 2013. "T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative stress." Environmental Toxicology and Pharmacology 36, no. 2: 493-500.

Evaluation study
Published: 01 January 2010 in Reproductive Biology and Endocrinology
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Very little information is known about the toxic effects of cadmium on somatic cells in mammalian testis. The objective of this study is to explore the toxicity of cadmium on piglet Sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were identified by oil red O staining and Fas ligand (FasL) expression as assayed by immunocytochemistry and expression of transferrin and androgen binding protein by RT-PCR. Sertoli cells were cultured in DMEM/F12 supplemented with 10% fetal calf serum in the absence or presence of various concentrations of cadmium chloride, or treatment with p38 MAPK inhibitor SB202190 and with cadmium chloride exposure. Apoptotic cells in seminiferous tubules of piglets were also performed using TUNEL assay in vivo. Cadmium chloride inhibited the proliferation of Piglet Sertoli cells as shown by MTT assay, and it increased malondialdehyde (MDA) but reduced superoxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) activity. Inhibitor SB202190 alleviated the proliferation inhibition of cadmium on piglet Sertoli cells. Comet assay revealed that cadmium chloride caused DNA damage of Piglet Sertoli cells and resulted in cell apoptosis as assayed by flow cytometry. The in vivo study confirmed that cadmium induced cell apoptosis in seminiferous tubules of piglets. Transmission electronic microscopy showed abnormal and apoptotic ultrastructure in Piglet Sertoli cells treated with cadmium chloride compared to the control. cadmium has obvious adverse effects on the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis, and aberrant morphology. This study thus offers novel insights into the toxicology of cadmium on male reproduction.

ACS Style

Ming Zhang; Zuping He; Lixin Wen; Jing Wu; Liyun Yuan; Yin Lu; Chengzhi Guo; Li Zhu; Sijun Deng; Hui Yuan. Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure. Reproductive Biology and Endocrinology 2010, 8, 97 -97.

AMA Style

Ming Zhang, Zuping He, Lixin Wen, Jing Wu, Liyun Yuan, Yin Lu, Chengzhi Guo, Li Zhu, Sijun Deng, Hui Yuan. Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure. Reproductive Biology and Endocrinology. 2010; 8 (1):97-97.

Chicago/Turabian Style

Ming Zhang; Zuping He; Lixin Wen; Jing Wu; Liyun Yuan; Yin Lu; Chengzhi Guo; Li Zhu; Sijun Deng; Hui Yuan. 2010. "Cadmium suppresses the proliferation of piglet Sertoli cells and causes their DNA damage, cell apoptosis and aberrant ultrastructure." Reproductive Biology and Endocrinology 8, no. 1: 97-97.