The early uplift of the Tibetan Plateau (TP) had a profound influence on the paleoenvironment and paleoclimate. However, we still have little information about the link between the paleoclimatic changes and flora ecosystem caused by the uplift. The Eocene fluvial-lacustrine sequences in the Gonjo Basin, eastern TP, provide excellent archives of the paleoecological and paleoclimatic responses to the surface uplift of the TP. In this study, we investigated a section of the middle Eocene Ranmugou Formation (47.8–>44 Ma) and used the sporomorphs assemblages, sedimentology, and geochemistry of the evaporites to reconstruct the paleoclimate and paleovegetation. The palynological assemblages and coexistence analysis reveal that the middle Eocene ecosystem in the Gonjo Basin was dominated by warm and humid temperate deciduous broad-leaved forests. The ⁸⁷Sr/⁸⁶Sr ratios (0.709942–0.710062) of all of the gypsum samples are higher than those of contemporaneous seawater, while the δ³⁴S values (10.3–11.0%) are much lower, indicating a lacustrine environment. Combined with published palynological and paleoelevation data for eastern Tibetan lacustrine basins, we infer that the paleoenvironment changed from warm and humid deciduous broad-leaved forests during 47.8–>44 Ma, to cool and arid temperate forest during 44–40 Ma in the Gonjo Basin, and to arid and cool steppe-desert vegetation in the late Eocene Nangqian Basin. The changes in the paleoclimate and vegetation were primarily driven by the surface uplift of the Central Watershed Mountain, the high topography of which blocked the moisture from the Asian Eocene monsoon.