Nitrogen (N) is an essential element in plant growth and productivity, and N fertilizer is therefore of prime importance in cultivated crops. The amount and timing of N application has economic and environmental implications and is consequently considered to be an important issue in precision agriculture. Spectral indices derived from handheld, airborne and spaceborne spectrometers are used for assessing N content. The majority of these indices are based on indirect indicators, mostly chlorophyll content, which is proven to be physiologically linked to N content. The current research aimed to explore the performance of new N spectral indices dependent upon the shortwave infrared (SWIR) region (1200–2500 nm), and particularly the 1510 nm band because it is related directly to N content. Traditional nitrogen indices (NIs) and four proposed new SWIR-based indices were tested with canopy-level spectral data obtained during two growing seasons in potato experimental plots in the northwest Negev, Israel. Above-ground biomass samples were collected at the same location of the spectral sampling to provide in-situ N content data. The performance of all indices was evaluated by three methods: (1) correlations between the existing and proposed indices and N as well as correlations among the indices themselves; (2) the root mean square error prediction (RMSEP) of the N content; and (3) the indices relative sensitivity (S _r) to the N content. The results reveal a firm advantage for the proposed SWIR-based indices in their ability to predict, and in their sensitivity to, N content. The best index is one that combines information from the 1510 and 660 nm bands but no significant differences were found among the new SWIR-based indices.

In-vivo foliar spectroscopy, also known as contact hyperspectral reflectance, enables rapid and non-destructive characterization of plant physiological status. This can be used to assess pathogen impact on plant condition both prior to and after visual symptoms appear. Challenging this capacity is the fact that dead tissue yields relatively consistent changes in leaf optical properties, negatively impacting our ability to distinguish causal pathogen identity. Here, we used in-situ spectroscopy to detect and differentiate Phytophthora infestans (late blight) and Alternaria solani (early blight) on potato foliage over the course of disease development and explored non-destructive characterization of contrasting disease physiology. Phytophthora infestans, a hemibiotrophic pathogen, undergoes an obligate latent period of two–seven days before disease symptoms appear. In contrast, A. solani, a necrotrophic pathogen, causes symptoms to appear almost immediately when environmental conditions are conducive. We found that respective patterns of spectral change can be related to these differences in underlying disease physiology and their contrasting pathogen lifestyles. Hyperspectral measurements could distinguish both P. infestans-infected and A. solani-infected plants with greater than 80% accuracy two–four days before visible symptoms appeared. Individual disease development stages for each pathogen could be differentiated from respective controls with 89–95% accuracy. Notably, we could distinguish latent P. infestans infection from both latent and symptomatic A. solani infection with greater than 75% accuracy. Spectral features important for late blight detection shifted over the course of infection, whereas spectral features important for early blight detection remained consistent, reflecting their different respective pathogen biologies. Shortwave infrared wavelengths were important for differentiation between healthy and diseased, and between pathogen infections, both pre- and post-symptomatically. This proof-of-concept work supports the use of spectroscopic systems as precision agriculture tools for rapid and early disease detection and differentiation tools, and highlights the importance of careful consideration of underlying pathogen biology and disease physiology for crop disease remote sensing.