Infrasound, a low-frequency physical stimulus from environmental and anthropogenic sources, is assumed to impact microvascular regulation, though the mechanism is not fully understood. This study examines the effects of bipolar infrasound exposure (1 Hz, 100 dB) on the sublingual microcirculation in mice, with a single 4-hour exposure and repeated 4-hour daily exposures for 14 days. Control mice were placed in the same chamber without infrasound exposure. Using sidestream dark-field (SDF) videomicroscopy and laser Doppler flowmetry (LDF), we assessed microvascular diameter oscillations (vasomotion) and flow changes (flowmotion), respectively. A single exposure significantly increased vasomotion oscillation amplitude in the low-frequency band, while changes in the very-low- and high-frequency bands were not significant. Flowmotion measured by LDF significantly increased in the very-low-frequency band after a single exposure, while the other frequency bands remained unchanged. In contrast, repeated exposure significantly increased vasomotion oscillation amplitude within the very-low-, low-, and high-frequency bands, while LDF-derived flowmotion did not change significantly. The findings suggest that repeated exposure to infrasound may induce irregular microvascular oscillations, primarily affecting vessel diameter rather than overall perfusion, warranting further exploration into related mechanisms.