To study the genetic diversity of phenotypic traits of natural Populus euphratica populations, we investigated the leaf, fruit and seed traits of the populations. The results of the phenotypic traits all showed that the variation among populations was larger than the variation within populations. The differentiation coefficients of phenotypic traits indicated that the variation among populations was the main source of population variation. The coefficient of variation of the phenotypic traits indicated that the leaf phenotypic traits directly exposed to the environment for a long time had the largest variation in the population. Correlation analysis between the phenotypic traits and environmental factors showed that geographical factors had a greater influence than climatic factors on phenotypic traits, and environmental factors had a certain degree of plasticity for phenotypic variation.

Pigskin, a major byproduct of pork processing, has high protein content and low fat, endowing it with considerable market value for food applications. In this study, bioaugmented fermentation with Latilactobacillus sakei YBZY-W5, a strain previously isolated from traditional fermented pigskin, was applied to pigskin to systematically evaluate its effects on physicochemical parameters, microbial community succession, and volatile flavor compound (VFC) profiles over 20 days. The results showed that moisture and pH significantly decreased, while total volatile basic nitrogen (TVB-N) and thiobarbituric acid reactive substances (TBARSs) increased with fermentation time. High-throughput sequencing revealed that Lactobacilli, Fusarium and Aspergillus dominated early fermentation and were gradually replaced by Bacillus, Hanseniaspora and Debaryomyces. A total of 493 VFCs were identified, among which terpenoids, heterocyclic compounds, and alcohols were the most abundant classes. Orthogonal partial least squares discriminant analysis (OPLS-DA) identified numerous differentially changed VFCs (DCVFCs) during fermentation. Odor activity value (OAV) analysis indicated that green, meaty, and woody notes dominated initially, while sour, floral, sweet, and fruity characteristics became increasingly prominent after fermentation. Pearson correlation analysis demonstrated significant associations between key microorganisms (Lactobacilli, Bacillus, Hanseniaspora, Debaryomyces) and DCVFCs (e.g., β-myrcene, ethyl hexanoate, hexanoic acid, ethyl ester, pyrazines). Collectively, bioaugmented fermentation with Ltb. sakei YBZY-W5 effectively modulated the physicochemical and microbial profiles of pigskin, enriched desirable flavor compounds, and reduced unpleasant odor, confirming its feasibility for producing high-quality fermented pigskin products. This study provides an experimental basis for the value-added utilization of pigskin and promotes sustainable development of the pork industry.

PIN-FORMED (PIN) auxin efflux carriers are key regulators of polar auxin transport, a process that controls plant organ morphogenesis and adaptive development. Nevertheless, genome-wide information on the PIN gene family in Populus euphratica Oliv. is limited, and how PIN homologs function during its distinctive heterophyllous leaf development remains unknown. Here, we systematically identified PIN genes throughout the P. euphratica genome and examined the subcellular localization and developmental effects of heterologously expressed PePIN1a in Arabidopsis thaliana. Bioinformatic analyses revealed 32 PePIN genes on 13 chromosomes and scaffolds, with segmental duplication contributing substantially to family expansion. Phylogenetic reconstruction resolved three PePIN subfamilies whose members exhibited conserved gene structures, protein motifs, and functional domains. Analysis of cis-acting elements indicated that most PePIN genes contained multiple elements responsive to hormones, light, and abiotic stress. Transcriptomic comparisons of four leaf morphotypes (linear, lanceolate, ovate, and broad-ovate) revealed relatively high PePIN1a expression in leaf tissues. Its greater expression in broad-ovate than in linear leaves prompted its selection for further functional validation. Subcellular localization assays revealed peripheral fluorescence from 35S::PePIN1a-GFP, consistent with association with the plasma membrane. In A. thaliana, PePIN1a overexpression significantly increased the number of rosette leaves relative to wild-type plants, while expression in the pin1 mutant rescued the reduced-leaf-number phenotype. These results systematically characterize the P. euphratica PIN family and show that PePIN1a alters rosette leaf number and exhibits PIN1-like activity in A. thaliana. PePIN1a is therefore a candidate for further studies of heterophyllous leaf development in P. euphratica. Because functional assays were conducted only in A. thaliana, its native role in P. euphratica heterophylly still requires validation.