Background/Objectives: Considering the antioxidant and antimicrobial properties attributed to compounds in Syzygium cumini extract, this research aimed to advance postoperative therapeutic innovations. Specifically, the study assessed the physicochemical properties of a film-forming solution (FFS) incorporated with S. cumini, evaluating its therapeutic potential for postoperative applications. Methods: The S. cumini extract was meticulously characterized to determine its chemical composition, with particular emphasis on the concentration of phenolic compounds. Antioxidant and antimicrobial assays were conducted to assess the extract’s efficacy in these domains. Following this, an FFS containing S. cumini was formulated and evaluated comprehensively for skin adhesion, mechanical and barrier properties, and thermal behavior. Results: The antioxidant and antimicrobial activities of the S. cumini extract demonstrated promising results, indicating its potential utility as an adjunct in postoperative care. The developed FFS exhibited favorable physicochemical properties for topical application, including adequate skin adhesion and appropriate pH levels. Moreover, chemical and thermal analyses confirmed the formulation’s stability and the retention of the extract’s beneficial properties. Conclusions: Overall, the findings suggest that the S. cumini-loaded FFS holds significant potential as a valuable therapeutic tool for post-surgical management.

The valorization of agro-industrial residues into higher-value-added products represents a promising strategy for the sustainable utilization of biomass and the advancement of circular economy approaches. Mango peels constitute an abundant residual biomass rich in bioactive compounds yet remain largely underutilized. This study aimed to valorize mango (Mangifera indica L.) peels by optimizing the extraction process of antioxidant compounds and chemically characterizing the resulting extracts. Different extraction techniques were initially compared in terms of extraction yield and antioxidant capacity, and high-shear homogenization-assisted extraction (HSH) for 20 min was selected for optimization based on its functional performance and operational practicality. A full factorial 3² design with a central point was employed to evaluate the effects of agitation speed and solid-to-solvent ratio. The optimized HSH conditions (15,000 rpm, a solid-to-solvent ratio of 1:10 (w/v), and 70% ethanol) yielded an extract with enhanced antioxidant capacity compared with conventional maceration for 7 days. Chemical characterization enabled the putative annotation of 28 compounds, with quercetin-3-O-pentoside and citric acid as the major constituents. The results demonstrate that optimized HSH enables the conversion of mango peels, an agro-industrial residual biomass, into antioxidant-rich extracts, contributing to biomass valorization and the development of higher-value-added products within a circular economy framework.

Fruits of Eugenia uniflora L. are rich in phenolic compounds and flavonoids, highlighting their potential as a source of bioactive compounds for nutraceutical development; however, extraction conditions can substantially influence the chemical composition and biological activity of the resulting extracts. This study evaluated the effects of extraction technique and solvent on the bioactive potential of E. uniflora fruit extracts. Powdered fruits were extracted by maceration, infusion, infusion followed by ethanol precipitation, and high-shear homogenization-assisted extraction (HSH) using water or 70% ethanol. Extracts were evaluated for yield, antioxidant capacity (DPPH, ABTS^•+, and FRAP), antimicrobial activity against six species, and chemical composition by UHPLC–MS/MS. HSH with 70% ethanol exhibited the highest DPPH (54.16 µmol Trolox equivalents g⁻¹) and FRAP (96.83 µmol Fe²⁺ g⁻¹) responses, whereas ethanolic maceration showed the highest ABTS^•+ scavenging capacity (170.59 µmol Trolox equivalents g⁻¹). The ethanolic extracts showed greater antibacterial activity, particularly against E. coli (MIC = 6.51 µg mL⁻¹) and S. epidermidis (MIC = 15.63 µg mL⁻¹). UHPLC–MS/MS revealed diverse metabolites, with citric acid, quinic acid, and quercitrin showing prominent chromatographic peak areas, alongside other flavonoids, phenolic compounds, and organic acids. Overall, HSH with 70% ethanol provided the most balanced performance, supporting its potential as an efficient strategy for obtaining E. uniflora extracts with nutraceutical potential.

Background/Objectives: Oral candidiasis is one of the most prevalent infections among denture wearers and is frequently associated with colonization by Candida albicans. In this context, natural products have been investigated as alternative therapeutic strategies, particularly Punica granatum peel extract and Cinnamomum cassia essential oil, which are recognized for their antimicrobial activity. This study aimed to develop and evaluate a mucoadhesive gel loaded with P. granatum and C. cassia for the treatment of oral candidiasis. Methods: The synergistic potential between the plant materials was assessed using the checkerboard method against C. albicans, and the inhibitory concentration was used as a parameter for the development of the mucoadhesive formulation. The gel was characterized and subsequently evaluated in denture-wearing patients with clinical manifestations of oral candidiasis for 14 days, with the results compared to treatment with nystatin. Results: The combination of plant materials exhibited a synergistic effect, with an inhibitory concentration of 0.1953 mg mL⁻¹ for both components. Clinical evaluation demonstrated a satisfactory therapeutic response in patients treated with the gel, with resolution of symptoms at the end of the treatment period. Statistically significant advantages were also observed regarding ease of application, absence of irritation, and reduced interference with speech and swallowing. In contrast, the group treated with nystatin showed only partial clinical improvement, with additional therapy required in some cases. Conclusions: These findings indicate that the mucoadhesive gel represents a promising topical strategy for the management of oral candidiasis in denture wearers. However, studies with longer follow-up periods are required to consolidate the clinical evidence.

Mikania glomerata (guaco) is widely used in traditional medicine due to its expectorant, antimicrobial, anti-inflammatory, antitussive, and bronchodilatory properties, which are mainly attributed to coumarins present in its leaves. Despite the growing interest in the use of plant growth-promoting bacteria (PGPB) in medicinal plants, the effects of individual inoculation and co-inoculation with Azospirillum brasilense and Pseudomonas fluorescens on the agronomic and phytochemical responses of M. glomerata remain poorly understood. In this context, we hypothesized that individual inoculation or co-inoculation with A. brasilense and P. fluorescens would differentially influence the agronomic performance and phytochemical profile of M. glomerata. Thus, this study evaluated the agronomic performance and phytochemical profile of guaco following inoculation with A. brasilense and P. fluorescens. A completely randomized 2 × 2 factorial design was employed, including single inoculation and co-inoculation treatments, with ten replicates per treatment. After seven months of cultivation, substrate biological indicators, plant biomass, nutrient content, hydroalcoholic extract yield, total phenolic content, and coumarin concentration were evaluated. P. fluorescens significantly increased plant biomass and shoot nitrogen content, whereas A. brasilense enhanced total phenolic content (7.81 µg gallic acid equivalents per gram of extract) and coumarin accumulation in guaco extracts (88 µg mL-1). Extractive yields ranged from 20.17% to 24.17%, with the highest value observed under co-inoculation. Overall, A. brasilense was the most effective treatment for stimulating the accumulation of pharmacologically relevant metabolites, while co-inoculation improved agronomic performance and extractive yield. These findings demonstrate that microbial inoculation is a sustainable biotechnological strategy for improving guaco cultivation and modulating the production of bioactive metabolites, contributing to the development of medicinal plant production systems with reduced dependence on chemical fertilizers.