Changes in climatic factors and rhizosphere microbiota led plants to adjust their metabolic strategies for survival under adverse environmental conditions. Changes in plant metabolites can mediate crop growth and development and interact with rhizosphere microbiota of the plant rhizosphere. To understand the interactions among environmental factors, rhizosphere microbiota, and metabolites of tobacco, a study was conducted by using integrated metagenomic and metabolomic strategies at four typical representative tobacco planting sites in Yunnan, China. The results showed that the agronomical and biochemical traits were significantly affected by temperature, precipitation (PREP), soil pH, and altitude. Correlation analyses revealed a significant positive correlation of temperature with length, width, and area of the leaf, while PREP correlated with plant height and effective leaf numbers. Furthermore, total sugar and reducing sugar contents of baked leaves were significantly higher, while the total nitrogen and total alkaloid levels were lower in tobacco leaves at site with low PREP. A total of 770 metabolites were detected with the highest number of different abundant metabolites (DMs) at Chuxiong (CX) with low PREP as compared to the other three sites, in which secondary metabolites were more abundant in both leaves and roots of tobacco. A total of 8,479 species, belonging to 2,094 genera with 420 individual bins (including 13 higher-quality bins) harboring 851,209 CDSs were detected. The phyla levels of microorganisms such as Euryarchaeota, Myxococcota, and Deinococcota were significantly enriched at the CX site, while Pseudomonadota was enriched at the high-temperature site with good PREP. The correlation analyses showed that the metabolic compounds in low-PREP site samples were positively correlated with Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora and negatively correlated with Amniculibacterium, Nordella, Noviherbaspirillum, and Limnobacter, suggesting that the recruitment of Diaminobutyricimonas, Nissabacter, Alloactinosynnema, and Catellatospora in the rhizosphere induces the production and accumulation of secondary metabolites (SMs) (e.g., nitrogen compounds, terpenoids, and phenolics) for increasing drought tolerance with an unknown mechanism. The results of this study may promote the production and application of microbial fertilizers and agents such as Diaminobutyricimonas and Alloactinosynnema to assemble synthetic microbiota community or using their gene resources for better cultivation of tobacco as well as other crops in drought environments.

Eocanthecona furcellata (Wolff, 1811) (Hemiptera: Pentatomidae: Asopinae), a predatory stinkbug, preys on diverse notorious insect pests, positioning it as a critical biocontrol agent in agricultural and forestry ecosystems with substantial regional application and promotion potential. We present a chromosome-level genome assembly of E. furcellata using PacBio HiFi long-read sequencing and Hi-C scaffolding technologies. The final genome spans 1.03 Gb, with a contig N50 of 2.35 Mb and a scaffold N50 of 135.69 Mb, achieving 97.0% BUSCO completeness with the Hemiptera_odb10 lineage dataset. Hi-C data resolved the assembly into 7 pseudochromosomes, representing the full karyotype. Repeat elements constitute 52.07% of the genome (approximately 520.07 Mb). We annotated 15,802 protein-coding genes, of which 90.72% were informatively assigned. This high-quality genome provides a foundational resource for advancing research on predatory bug biology, including genetic adaptations for digestion, molecular mechanisms of prey detection, and biocontrol optimization through functional genomics.

Eocanthecona furcellata (Wolff) (Hemiptera: Pentatomidae: Asopinae) captures and feeds on a wide range of insect pests, making it an important biocontrol agent in agriculture and forestry with significant regional application and promotional potentials. This review summarizes current research information on E. furcellata focusing on taxonomy, morphological characteristics involved in predation, its natural prey, mass rearing, regional application and potential, integrated pest management (IPM) usages, ecological safety evaluations, as well as future prospects for its use in IPM programs.