Hexafluoropropylene oxide trimer acid (HFPO-TA) and hexafluoropropylene oxide tetramer acid (HFPO-TeA) are two novel alternatives of perfluorooctanoic acid (PFOA). However, their toxicokinetics and accumulation mechanisms in fish are still unknown. This study provides the first line of in vivo uptake and depuration kinetic, bioaccumulation mechanism and tissue-specific distribution for HFPO-TA and HFPO-TeA, upon a 28-day water exposure and a 14-day depuration in zebrafish (Danio rerio). HFPO-TeA and HFPO-TA could quickly accumulate in zebrafish, and the highest concentrations of HFPO-TeA (15.4 ± 1.6 nmol/g ww), HFPO-TA (4.95 ± 0.19 nmol/g ww) and PFOA (0.47 ± 0.03 nmol/g ww) were consistently present in the blood, which was followed by liver, kidney, intestine, gill, gonad and brain, while the lowest were observed in the muscle (1.01 ± 0.11, 0.16 ± 0.02, and 0.01 ± 0.001 nmol/g ww, respectively), indicating both higher accumulation potentials of both HFPO homologs than their predecessor PFOA. The tissue protein content, rather than lipid content, played an enhancing role in the enrichment of three target chemicals, exhibiting a significant positive correlation (r = 0.735, p = 0.038 for HFPO-TeA; r = 0.770, p = 0.026 for HFPO-TA and r = 0.942, p = 0.001 for PFOA) between the tissue bioconcentration factor (BCF) and the protein contents in corresponding tissues. This phenomenon was validated by the equilibrium dialysis experiment, molecular docking analysis and molecular dynamics simulation, which consistently indicated that the binding affinities of serum and liver proteins were greatest with HFPO-TeA, followed by HFPO-TA and least with PFOA. These results suggested that the binding of the target chemicals to specific proteins determined their tissue-specific accumulation potentials. Nontarget screening by high resolution mass spectrometry (HRMS) did not identify suspicious degradation products for HFPO-TA, implying the strong persistence of HFPO-TA in fish.

Photocatalytic technology is considered an ideal approach for clean energy conversion and environmental pollution applications. In this work, a bifunctional BiOBr/ZIF-8/ZnO photocatalyst was proposed for removing phenols in wastewater and generating hydrogen peroxide. Insights from scanning electron microscopy measurements revealed the well-dispersion of ZIF-8/ZnO was on the BiOBr layer, which could effectively prevent agglomeration of ZIF-8 and facilitate the separation of carriers. In addition, the optimal H₂O₂ yield of the BiOBr/ZIF-8/ZnO sample could reach 116 mmol·L⁻¹·g⁻¹ within 2 h, much higher than that of pure BiOBr (with the value of 82 mmol·L⁻¹·g⁻¹). The optimal BiOBr/ZIF-8/ZnO sample could also remove 90% of the phenol or bisphenol A in 2 h, and its kinetic constants were 3.8 times and 2.3 times that of pure BiOBr, respectively. Based on the analysis of the various experimental characterizations, the photocatalytic mechanism of the S-scheme BiOBr/ZIF-8/ZnO composite for the degradation of phenolic pollutants and generation of H₂O₂ was proposed. The formation of the heterojunction and the oxygen vacancy work together to significantly improve its photocatalytic efficiency. In addition, the BiOBr/ZIF-8/ZnO catalyst has a certain impact on the degradation of phenol in actual wastewater, providing a way to effectively remove refractory pollutants and generate H₂O₂ in actual water.