This page has only limited features, please log in for full access.
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating fungal diseases of wheat worldwide. It is essential to discover more sources of stripe rust resistance genes for wheat breeding programs. Specific locus amplified fragment sequencing (SLAF-seq) is a powerful tool for the construction of high-density genetic maps. In this study, a set of 200 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Chuanmai 42 (CH42) and Chuanmai 55 (CH55) was used to construct a high-density genetic map and to identify quantitative trait loci (QTLs) for stripe rust resistance using SLAF-seq technology. A genetic map of 2828.51 cM, including 21 linkage groups, contained 6732 single nucleotide polymorphism markers (SNP). Resistance QTLs were identified on chromosomes 1B, 2A, and 7B; Qyr.saas-7B was derived from CH42, whereas Qyr.saas-1B and Qyr.saas-2A were from CH55. The physical location of Qyr.saas-1B, which explained 6.24-34.22% of the phenotypic variation, overlapped with the resistance gene Yr29. Qyr.saas-7B accounted for up to 20.64% of the phenotypic variation. Qyr.saas-2A, a minor QTL, was found to be a likely new stripe rust resistance locus. A significant additive effect was observed when all three QTLs were combined. The combined resistance genes could be of value in breeding wheat for stripe rust resistance.
Manyu Yang; Guangrong Li; Hongshen Wan; Liping Li; Jun Li; Wuyun Yang; Zongjun Pu; Zujun Yang; Ennian Yang. Identification of QTLs for Stripe Rust Resistance in a Recombinant Inbred Line Population. International Journal of Molecular Sciences 2019, 20, 3410 .
AMA StyleManyu Yang, Guangrong Li, Hongshen Wan, Liping Li, Jun Li, Wuyun Yang, Zongjun Pu, Zujun Yang, Ennian Yang. Identification of QTLs for Stripe Rust Resistance in a Recombinant Inbred Line Population. International Journal of Molecular Sciences. 2019; 20 (14):3410.
Chicago/Turabian StyleManyu Yang; Guangrong Li; Hongshen Wan; Liping Li; Jun Li; Wuyun Yang; Zongjun Pu; Zujun Yang; Ennian Yang. 2019. "Identification of QTLs for Stripe Rust Resistance in a Recombinant Inbred Line Population." International Journal of Molecular Sciences 20, no. 14: 3410.
To determine the composition of chromosome aberrations in a wheat‒Dasypyrum breviaristatum substitution line with seeds treated by a dose of gamma-rays (200 Gy), sequential non-denaturing fluorescence in situ hybridization (ND-FISH) with multiple oligonucleotide probes was used to screen individual plants of the mutagenized progenies. We identified 122 types of chromosome rearrangements, including centromeric, telomeric, and intercalary chromosome translocations from a total of 772 M1 and 872 M2 plants. The frequency of reciprocal translocations between B- and D-chromosomes was higher than that between A- and D-chromosomes. Eight translocations between D. breviaristatum and wheat chromosomes were also detected. The 13 stable plants with multiple chromosome translocations displayed novel agronomic traits. The newly developed materials will enhance wheat breeding programs through wheat‒Dasypyrum introgression and also facilitate future studies on the genetic and epigenetic effects of translocations in wheat genomics.
Hongjin Wang; Zhihui Yu; Guangrong Li; Zujun Yang. Diversified Chromosome Rearrangements Detected in a Wheat‒Dasypyrum breviaristatum Substitution Line Induced by Gamma-Ray Irradiation. Plants 2019, 8, 175 .
AMA StyleHongjin Wang, Zhihui Yu, Guangrong Li, Zujun Yang. Diversified Chromosome Rearrangements Detected in a Wheat‒Dasypyrum breviaristatum Substitution Line Induced by Gamma-Ray Irradiation. Plants. 2019; 8 (6):175.
Chicago/Turabian StyleHongjin Wang; Zhihui Yu; Guangrong Li; Zujun Yang. 2019. "Diversified Chromosome Rearrangements Detected in a Wheat‒Dasypyrum breviaristatum Substitution Line Induced by Gamma-Ray Irradiation." Plants 8, no. 6: 175.
A large proportion of the genomes of grasses is comprised of tandem repeats (TRs), which include satellite DNA. A mini-satellite DNA sequence with a length of 44 bp, named Ta-3A1, was found to be highly accumulated in wheat genome, as revealed by a comprehensive sequence analysis. The physical distribution of Ta-3A1 in chromosomes 3A, 5A, 5B, 5D, and 7A of wheat was confirmed by nondenaturing fluorescence in situ hybridization (ND-FISH) after labeling the oligonucleotide probe. The analysis of monomer variants indicated that rapid sequence amplification of Ta-3A1 occurred first on chromosomes of linkage group 5, then groups 3 and 7. Comparative ND-FISH analysis suggested that rapid changes occurred in copy number and chromosomal locations of Ta-3A1 among the different species in the tribe Triticeae, which may have been associated with chromosomal rearrangements during speciation and polyploidization. The labeling and subsequent use of Ta-3A1 by ND-FISH may assist in the precise identification and documentation of novel wheat germplasm engineered by chromosome manipulation.
Tao Lang; Guangrong Li; Zhihui Yu; Jiwei Ma; Qiheng Chen; Ennian Yang; Zujun Yang. Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species. Agronomy 2019, 9, 60 .
AMA StyleTao Lang, Guangrong Li, Zhihui Yu, Jiwei Ma, Qiheng Chen, Ennian Yang, Zujun Yang. Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species. Agronomy. 2019; 9 (2):60.
Chicago/Turabian StyleTao Lang; Guangrong Li; Zhihui Yu; Jiwei Ma; Qiheng Chen; Ennian Yang; Zujun Yang. 2019. "Genome-Wide Distribution of Novel Ta-3A1 Mini-Satellite Repeats and Its Use for Chromosome Identification in Wheat and Related Species." Agronomy 9, no. 2: 60.
The wild species, Thinopyrum intermedium. (Genome StStJSJSJJ), serves as a valuable germplasm resource providing novel genes for wheat improvement. In the current study, non-denaturing fluorescence in situ hybridization (ND-FISH) with multiple probes and comparative molecular markers were applied to characterize two wheat-Th. intermedium chromosome additions. Sequential ND-FISH with new labeled Th. intermedium specific oligo-probes were used to precisely determine the chromosomal constitution of Th. intermedium, wheat—Th. intermedium partial amphiploids and addition lines Hy36 and Hy37. The ND-FISH results showed that the added JS-St translocated chromosomes in Hy36 had minor Oligo-5S ribosomal DNA (rDNA) signals at the short arm, while a pair of J-St chromosomes in Hy37 had major Oligo-pTa71 and minor Oligo-5S rDNA signals. The 90K SNP array and PCR-based molecular markers that mapped on wheat linkage group 5 and 3 facilitated the identification of Thinopyrum chromosome introgressions in the addition lines, and confirmed that added chromosomes in Hy36 and Hy37 were 5JSS.3StS and 5JS.3StS, respectively. Complete coding sequences at the paralogous puroindoline-a (Pina) loci from Th. intermedium were cloned and localized on the short arm of chromosome 5JS of Hy36. Line Hy36 showed a reduction in the hardness index, which suggested that Th. intermedium-specific Pina gene sequences may be associated with the softness trait in wheat background. The molecular cytogenetic identification of novel wheat—Th. intermedium derivatives indicated that the frequent chromosome rearrangement occurred in the progenies of wheat-Thinopyrum hybridization. The new wheat-Thinopyrum derived lines may increase the genetic diversity for wheat breeding.
Zhihui Yu; Hongjin Wang; Yunfang Xu; Yongshang Li; Tao Lang; Zujun Yang; Guangrong Li. Characterization of Chromosomal Rearrangement in New Wheat—Thinopyrum intermedium Addition Lines Carrying Thinopyrum—Specific Grain Hardness Genes. Agronomy 2019, 9, 18 .
AMA StyleZhihui Yu, Hongjin Wang, Yunfang Xu, Yongshang Li, Tao Lang, Zujun Yang, Guangrong Li. Characterization of Chromosomal Rearrangement in New Wheat—Thinopyrum intermedium Addition Lines Carrying Thinopyrum—Specific Grain Hardness Genes. Agronomy. 2019; 9 (1):18.
Chicago/Turabian StyleZhihui Yu; Hongjin Wang; Yunfang Xu; Yongshang Li; Tao Lang; Zujun Yang; Guangrong Li. 2019. "Characterization of Chromosomal Rearrangement in New Wheat—Thinopyrum intermedium Addition Lines Carrying Thinopyrum—Specific Grain Hardness Genes." Agronomy 9, no. 1: 18.
The objective of this research was to characterize the high level of resistance to stripe that has been observed in the released wheat cultivar, Chuanmai45. A combination of classic genetic analysis, molecular and cytogenetic methods were used to characterize resistance in an F2 population derived from Chuanmai45 and the susceptible Chuanmai42. Inheritance of resistance was shown to be conferred by two genes in Chuanmai45. Fluorescence in situ hybridization (FISH) was used along with segregation studies to show that one gene was located on a 1RS.1BL translocation. Molecular markers were employed to show that the other locus was located on chromosome 4B. The defeated gene, Yr24/26, on chromosome 1BL was present in the susceptible parent and lines that recombined this gene with the 1RS.1BL translocation were identified. The germplasm, loci, and associated markers identified in this study will be useful for application in breeding programs utilizing marker-assisted selection.
Ennian Yang; Guangrong Li; Liping Li; Zhenyu Zhang; Wuyun Yang; Yunliang Peng; Yongqing Zhu; Zujun Yang; Garry M. Rosewarne. Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45. International Journal of Molecular Sciences 2016, 17, 601 .
AMA StyleEnnian Yang, Guangrong Li, Liping Li, Zhenyu Zhang, Wuyun Yang, Yunliang Peng, Yongqing Zhu, Zujun Yang, Garry M. Rosewarne. Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45. International Journal of Molecular Sciences. 2016; 17 (4):601.
Chicago/Turabian StyleEnnian Yang; Guangrong Li; Liping Li; Zhenyu Zhang; Wuyun Yang; Yunliang Peng; Yongqing Zhu; Zujun Yang; Garry M. Rosewarne. 2016. "Characterization of Stripe Rust Resistance Genes in the Wheat Cultivar Chuanmai45." International Journal of Molecular Sciences 17, no. 4: 601.
The uncultivated species Dasypyrum breviaristatum carries novel diseases resistance and agronomically important genes of potential use for wheat improvement. The development of new wheat-D. breviaristatum derivatives lines with disease resistance provides an opportunity for the identification and localization of resistance genes on specific Dasypyrum chromosomes. The comparison of wheat-D. breviaristatum derivatives to the wheat-D. villosum derivatives enables to reveal the genomic divergence between D. breviaristatum and D. villosum. The mitotic metaphase of the wheat- D. breviaristatum partial amphiploid TDH-2 and durum wheat -D. villosum amphiploid TDV-1 were studied using multicolor fluorescent in situ hybridization (FISH). We found that the distribution of FISH signals of telomeric, subtelomeric and centromeric regions on the D. breviaristatum chromosomes was different from those of D. villosum chromosomes by the probes of Oligo-pSc119.2, Oligo-pTa535, Oligo-(GAA)7 and Oligo-pHv62-1. A wheat line D2139, selected from a cross between wheat lines MY11 and TDH-2, was characterized by FISH and PCR-based molecular markers. FISH analysis demonstrated that D2139 contained 44 chromosomes including a pair of D. breviaristatum chromosomes which had originated from the partial amphiploid TDH-2. Molecular markers confirmed that the introduced D. breviaristatum chromosomes belonged to homoeologous group 7, indicating that D2139 was a 7V(b) disomic addition line. The D2139 displayed high resistance to wheat stripe rust races at adult stage plant, which may be inherited from, D. breviaristatum chromosome 7V(b). The study present here revealed that the large divergence between D. breviaristatum and D. villosum with respected to the organization of different repetitive sequences. The identified wheat- D. breviaristatum chromosome addition line D2139 will be used to produce agronomically desirable germplasm for wheat breeding.
Guangrong Li; Dan Gao; Hongjun Zhang; Jianbo Li; Hongjin Wang; Shixiao La; Jiwei Ma; Zujun Yang. Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species. Molecular Cytogenetics 2016, 9, 6 .
AMA StyleGuangrong Li, Dan Gao, Hongjun Zhang, Jianbo Li, Hongjin Wang, Shixiao La, Jiwei Ma, Zujun Yang. Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species. Molecular Cytogenetics. 2016; 9 (1):6.
Chicago/Turabian StyleGuangrong Li; Dan Gao; Hongjun Zhang; Jianbo Li; Hongjin Wang; Shixiao La; Jiwei Ma; Zujun Yang. 2016. "Molecular cytogenetic characterization of Dasypyrum breviaristatum chromosomes in wheat background revealing the genomic divergence between Dasypyrum species." Molecular Cytogenetics 9, no. 1: 6.
Mutations induced by radiation are widely used for developing new varieties of plants. To better understand the frequency and pattern of irradiation-induced chromosomal rearrangements, we irradiated the dry seeds of Chinese Spring (CS)-Dasypyrum villosum nullisomic-tetrasomic (6A/6D) addition (6V) line (2n = 44), WD14, with 60Co-γ-rays at dosages of 100, 200, and 300 Gy. The M0 and M1 generations were analyzed using Feulgen staining and non-denaturing fluorescence in situ hybridization (ND-FISH) by using oligonucleotide probes. Abnormal mitotic behavior and chromosomes with structural changes were observed in the M0 plants. In all, 39 M1 plants had structurally changed chromosomes, with the B genome showing the highest frequency of aberrations and tendency to recombine with chromosomes of the D genome. In addition, 19 M1 plants showed a variation in chromosome number. The frequency of chromosome loss was considerably higher for 6D than for the alien chromosome 6V, indicating that 6D is less stable after irradiation. Our findings suggested that the newly obtained γ-induced genetic materials might be beneficial for future wheat breeding programs and functional gene analyses.
Jie Zhang; Yun Jiang; Yuanlin Guo; Guangrong Li; Zujun Yang; Delin Xu; Pu Xuan. Identification of Novel Chromosomal Aberrations Induced by 60Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines. International Journal of Molecular Sciences 2015, 16, 29787 -29796.
AMA StyleJie Zhang, Yun Jiang, Yuanlin Guo, Guangrong Li, Zujun Yang, Delin Xu, Pu Xuan. Identification of Novel Chromosomal Aberrations Induced by 60Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines. International Journal of Molecular Sciences. 2015; 16 (12):29787-29796.
Chicago/Turabian StyleJie Zhang; Yun Jiang; Yuanlin Guo; Guangrong Li; Zujun Yang; Delin Xu; Pu Xuan. 2015. "Identification of Novel Chromosomal Aberrations Induced by 60Co-γ-Irradiation in Wheat-Dasypyrum villosum Lines." International Journal of Molecular Sciences 16, no. 12: 29787-29796.
A novel Dasypyrum species, Dasypyrum breviaristatum, serves as a valuable source of useful genes for wheat improvement. The development and characterization of new wheat—D. breviaristatum introgression lines is important to determine the novel gene(s) on specific chromosome(s). We first used multi-color fluorescence in situ hybridization (FISH) to identify the individual D. breviaristatum Vb chromosomes in a common wheat—D. breviaristatum partial amphiploid, TDH-2. The FISH patterns of D. breviaristatum chromosomes were different from those of D. villosum chromosomes. Lines D2146 and D2150 were selected from a cross between wheat line MY11 and wheat—D. breviaristatum partial amphiploid TDH-2, and they were characterized by FISH and PCR-based molecular markers. We found that D2150 was a monosomic addition line for chromosome 5Vb of D. breviaristatum, while D2146 had the 5VbL chromosome arm translocated with wheat chromosome 5AS. Molecular marker analysis confirmed that the introduced D. breviaristatum chromosome 5VbL translocation possessed a duplicated region homoeologous to 5AS, revealing that the 5AS.5VbL translocation may not functionally compensate well. The dwarfing and the pre-harvest re-growth habits observed in the wheat—D. breviaristatum chromosome 5Vb derivatives may be useful for future development of perennial growth wheat lines.
Hongjun Zhang; Guangrong Li; Donghai Li; Dan Gao; Jie Zhang; Ennian Yang; Zujun Yang. Molecular and Cytogenetic Characterization of New Wheat—Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit. Genes 2015, 6, 1242 -1255.
AMA StyleHongjun Zhang, Guangrong Li, Donghai Li, Dan Gao, Jie Zhang, Ennian Yang, Zujun Yang. Molecular and Cytogenetic Characterization of New Wheat—Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit. Genes. 2015; 6 (4):1242-1255.
Chicago/Turabian StyleHongjun Zhang; Guangrong Li; Donghai Li; Dan Gao; Jie Zhang; Ennian Yang; Zujun Yang. 2015. "Molecular and Cytogenetic Characterization of New Wheat—Dasypyrum breviaristatum Derivatives with Post-Harvest Re-Growth Habit." Genes 6, no. 4: 1242-1255.
The unique 75 K γ-secalins encoded by Sec2 loci in Secale species is composed of almost half rye storage proteins. The chromosomal location of Sec2 loci in wild Secale species, Secale africanum, was carried out by the wheat—S. africanum derivatives, which were identified by genomic in situ hybridization and multi-color fluorescence in situ hybridization. The Sec2 gene-specific PCR analysis indicated that the S. cereale Sec2 was located onchromosome 2R, while the S. africanum Sec2 was localized on chromosome 6Rafr of S. africanum. A total of 38 Sec2 gene sequences were isolated from S. africanum, S. cereale and S. sylvestre by PCR-based cloning. Phylogenetic analysis showed that S. africanum Sec2 diverged from S. cereale Sec2 approximately 2–3 million years ago. The illegitimate recombination of chromosome 2R–6R involving the Sec2 loci region may accelerate sequence variation during evolutionary process from wild to cultivated Secale species.
Guangrong Li; Hongjun Zhang; Li Zhou; Dan Gao; Mengping Lei; Jie Zhang; Zujun Yang. Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species. International Journal of Molecular Sciences 2015, 16, 8324 -8336.
AMA StyleGuangrong Li, Hongjun Zhang, Li Zhou, Dan Gao, Mengping Lei, Jie Zhang, Zujun Yang. Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species. International Journal of Molecular Sciences. 2015; 16 (12):8324-8336.
Chicago/Turabian StyleGuangrong Li; Hongjun Zhang; Li Zhou; Dan Gao; Mengping Lei; Jie Zhang; Zujun Yang. 2015. "Molecular Characterization of Sec2 Loci in Wheat—Secale africanum Derivatives Demonstrates Genomic Divergence of Secale Species." International Journal of Molecular Sciences 16, no. 12: 8324-8336.
A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.
Haixian Zhan; Xiaojun Zhang; Guangrong Li; Zhihui Pan; Jin Hu; Xin Li; Linyi Qiao; Juqing Jia; Huijuan Guo; Zhijian Chang; Zujun Yang. Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust. International Journal of Molecular Sciences 2015, 16, 2162 -2173.
AMA StyleHaixian Zhan, Xiaojun Zhang, Guangrong Li, Zhihui Pan, Jin Hu, Xin Li, Linyi Qiao, Juqing Jia, Huijuan Guo, Zhijian Chang, Zujun Yang. Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust. International Journal of Molecular Sciences. 2015; 16 (1):2162-2173.
Chicago/Turabian StyleHaixian Zhan; Xiaojun Zhang; Guangrong Li; Zhihui Pan; Jin Hu; Xin Li; Linyi Qiao; Juqing Jia; Huijuan Guo; Zhijian Chang; Zujun Yang. 2015. "Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust." International Journal of Molecular Sciences 16, no. 1: 2162-2173.
Wild barley eibi1 mutant with HvABCG31 gene mutation has low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. To better understand how such a mutant plant survives, we performed a genome-wide gene expression analysis. The leaf transcriptomes between the near-isogenic lines eibi1 and the wild type were compared using the 22-k Barley1 Affymetrix microarray. We found that the pleiotropic effect of the single gene HvABCG31 mutation was linked to the co-regulation of metabolic processes and stress-related system. The cuticle development involved cytochrome P450 family members and fatty acid metabolism pathways were significantly up-regulated by the HvABCG31 mutation, which might be anticipated to reduce the levels of cutin monomers or wax and display conspicuous cuticle defects. The candidate genes for responses to stress were induced by eibi1 mutant through activating the jasmonate pathway. The down-regulation of co-expressed enzyme genes responsible for DNA methylation and histone deacetylation also suggested that HvABCG31 mutation may affect the epigenetic regulation for barley development. Comparison of transcriptomic profiling of barley under biotic and abiotic stresses revealed that the functions of HvABCG31 gene to high-water loss rate might be different from other osmotic stresses of gene mutations in barley. The transcriptional profiling of the HvABCG31 mutation provided candidate genes for further investigation of the physiological and developmental changes caused by the mutant.
Zujun Yang; Tao Zhang; Tao Lang; Guangrong Li; Guoxiong Chen; Eviatar Nevo. Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways. International Journal of Molecular Sciences 2013, 14, 20478 -20491.
AMA StyleZujun Yang, Tao Zhang, Tao Lang, Guangrong Li, Guoxiong Chen, Eviatar Nevo. Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways. International Journal of Molecular Sciences. 2013; 14 (10):20478-20491.
Chicago/Turabian StyleZujun Yang; Tao Zhang; Tao Lang; Guangrong Li; Guoxiong Chen; Eviatar Nevo. 2013. "Transcriptome Comparative Profiling of Barley eibi1 Mutant Reveals Pleiotropic Effects of HvABCG31 Gene on Cuticle Biogenesis and Stress Responsive Pathways." International Journal of Molecular Sciences 14, no. 10: 20478-20491.