Land-use intensification is a major driver of biodiversity loss. However, understanding how different components of land use drive biodiversity loss requires the investigation of multiple trophic levels across spatial scales. Using data from 150 agricultural grasslands in central Europe, we assess the influence of multiple components of local- and landscape-level land use on more than 4,000 above- and belowground taxa, spanning 20 trophic groups. Plot-level land-use intensity is strongly and negatively associated with aboveground trophic groups, but positively or not associated with belowground trophic groups. Meanwhile, both above- and belowground trophic groups respond to landscape-level land use, but to different drivers: aboveground diversity of grasslands is promoted by diverse surrounding land-cover, while belowground diversity is positively related to a high permanent forest cover in the surrounding landscape. These results highlight a role of landscape-level land use in shaping belowground communities, and suggest that revised agroecosystem management strategies are needed to conserve whole-ecosystem biodiversity.

The development of a dual-wavelength full-waveform terrestrial laser scanner to measure the three-dimensional structure of forest canopies is described, and field measurements used to evaluate and test the instrument measurement characteristics. The Salford Advanced Laser Canopy Analyser (SALCA) measures the full-waveform of backscattered radiation at two laser wavelengths, one in the near-infrared (1063nm) and one in the shortwave infrared (1545nm). The instrument is field-portable and measures up to nine million waveforms, at the two wavelengths, across a complete hemisphere above the instrument. SALCA was purpose-built to measure structural characteristics of forest canopies and this paper reports the first results of field-based data collection using the instrument. Characteristics of the waveforms, and waveform data processing are outlined, applications of dual wavelength measurements are evaluated, and field deployment of the instrument at a forest test site described. Preliminary instrument calibration results are presented and challenges in extracting useful information on forest structure are highlighted. Full-waveform multiple-wavelength terrestrial laser scanners are likely to provide more detailed and more accurate forest structural measurement in the future. This research demonstrates how SALCA provides a key step to develop, test and apply this new technology in a range of forest-related problems