Habitat fragmentation due to anthropogenic pressures threats functional connectivity across landscapes for flying mammals. Tadarida brasiliensis depends on nocturnal movement corridors linking refuge and foraging areas, yet these pathways are increasingly constrained in semi-arid regions of northern Mexico. This study developed and analyzed the potential ecological corridors connecting the main colony of T. brasiliensis located in Santa Eulalia with the Irrigation District 005 Delicias, in Chihuahua, Mexico. We integrated multi-source geospatial data within a geographic information system, including wind speed, terrain slope, normalized difference vegetation index, land surface temperature, distance to rivers, landscape aggregation, nighttime lighting, and distance to roads, power lines, and human settlements. Landscape resistance to movement was assessed using a combined framework based on the Analytic Hierarchy Process, the InVEST-Habitat Quality model, and Least Cost Path analysis, generating composite resistance. Five potential corridors were identified, with ranges of lengths and CWD:EucD ratios of 6.8–34.0 km and 20.4–51.3, respectively, reflecting variable cumulative resistance along pathways. Nighttime lighting and proximity to urban areas were major contributors to high resistance, particularly within urban and agricultural environments. The identified corridor network provides a spatial representation of potential routes and supports landscape-level conservation planning to mitigate anthropogenic pressures and maintain functional connectivity.