Cudrania tricuspidata is a traditional medicinal herb in East Asia. The compounds of plants vary depending on environmental factors, such as soil, temperature, drainage, and so on. However, few to no studies have been done on the correlation among environment, growth, and compounds in C. tricuspidata. Thus, we aimed to investigate their relationship. Samples of C. tricuspidata fruit and cultivation soil were collected from 28 cultivation sites in October 2021. Six growth characteristics, eleven soil physicochemical properties, seven meteorological data points, and three active compounds were investigated in this study. We developed and validated an optimized method for quantifying active compounds using UPLC and performed correlation analysis of the environment, growth characteristics, and active compounds. The UPLC-UV method for determining active compounds was validated by measuring the linearity, LOD, LOQ, precision, and accuracy using UPLC. The LOD and LOQ were 0.01–0.03 µg/mL and 0.04–0.09 µg/mL, respectively. The precision was acceptable with RSD% values less than 2%. The recoveries ranged from 97.25 to 104.98% with RSD values <2%, within the acceptable limits. The active compounds were negatively correlated with the size of the fruit, and the growth characteristics were negatively correlated with some environmental factors. The results of this study can be used as basic data for the standard cultural practices and quality control of C. tricuspidata fruits.

The growth characteristics and active compounds of plants are influenced by various environmental factors, including light, temperature, soil, and precipitation. These factors interact with each plant in a unique way. In this study, we collected fruit and soil samples from 17 cultivation sites in October 2020 to investigate the relationship among environmental factors, growth characteristics, and active compounds of H. dulcis seed and fruit. We developed an optimized method for quantifying active compounds using ultra-performance liquid chromatography and performed correlation analyses with the surveyed environmental factors. Results showed that the size and fresh weight of H. dulcis fruits were positively correlated with exchangeable Mg, Na, and silt texture proportion in the soil. The temperature was positively correlated with sugar content in the fruits but negatively correlated with fruit weight, and precipitation was negatively correlated with fruit size. Results also showed a positive correlation between the active compounds and growth characteristics such as fruit length, seed number per fruit, and fresh weight. The results of this study can be used as basic data for the cultivation and quality control of H. dulcis fruits.

Betaine is a useful compound that has various activities and is the marker compound of Lycium chinense fruit in Korean Pharmacopoeia. we seek to support the stable production of medicinal goji berries, which have significant potential in the pharmaceutical industry due to their high values, and to provide foundational data for consistent quality control. This study’s purpose was to examine the correlation among betaine content, environmental variables, and the growth characteristics of L. chinense fruits. The fruits were collected from 25 cultivation sites across South Korea. We investigated five growth characteristics and betaine contents in L. chinense fruits and twelve soil physicochemical properties, and seven meteorological data at cultivation sites. The fruit’s growth characteristics included a length of 15.62–26.49 mm, a width of 7.09–11.38 mm, a fresh weight of 0.73–1.62 g, and a sugar content of 11.10–19.62 Brix°. Its betaine content ranged from 0.54% to 0.97%. The betaine content was positively correlated with electrical conductivity (0.327 **), exchangeable potassium (0.314 **), and sodium (0.259 *) and negatively correlated with annual average minimum temperature (−0.256 *) and annual average temperature (−0.242 *). Also, betaine showed a positive correlation with the length of the fruit (0.294 *) and the fresh weight of the fruit (0.238 *). These results can be used to find the best cultivation method and to manage quality control for the highly economical L. chinense fruit.

Adenophora triphylla, a perennial plant widely used in traditional medicine, produces secondary metabolites like lupenone and β-sitosterol. Understanding how environmental factors influence the concentrations of these metabolites is crucial for optimizing their medicinal use and cultivation. This study examined the relationships between soil physicochemical properties, climatic variables, and the growth and metabolite content of A. triphylla roots across 17 cultivation sites in South Korea. Methods included UHPLC-MS/MS analysis for quantifying lupenone and β-sitosterol, alongside correlation analysis of growth traits and environmental factors. The results showed that higher soil pH and calcium/magnesium content were positively correlated with the fresh weight of the root, whereas lower temperatures increased biomass accumulation. Additionally, lupenone was significantly associated with the elongation of various root growth, whereas β-sitosterol showed a correlation only with the diameter of the taproot. These findings suggest that environmental factors, particularly soil pH, Ca²⁺, Mg²⁺, and temperature, play key roles in influencing both the growth and metabolite production of A. triphylla, providing valuable insights into improving its medicinal and agronomic potential.

The rhizome of Atractylodes macrocephala, a perennial herb in the Asteraceae family, is valued for its bioactive atractylenolides, but achieving consistent quality in cultivation is challenging. This study aimed to decipher how environmental factors differentially regulate its biomass and atractylenolide content. We sampled from 22 Korean cultivation sites and performed correlation analyses, rigorously controlled by a False Discovery Rate (FDR) correction. Our analysis revealed that the environmental networks governing quantitative growth and qualitative composition are largely independent. While growth was weakly correlated with environmental factors, likely due to suboptimal temperatures at our sites, atractylenolide content was robustly associated with soil properties and climate. Specifically, soil texture was a dominant factor, with sand content showing a strong negative correlation (−0.717 ***) with total atractylenolides, whereas silt (0.675 ***) and clay (0.622 ***) had strong positive correlations. Additionally, cation exchange capacity (0.517 *) and temperature were positively correlated, while relative humidity showed a negative correlation (−0.553 **). This decoupling suggests that optimizing yield and phytochemical quality requires distinct cultivation strategies, providing a foundational framework for developing site-specific practices and quality control for this high-value medicinal herb.

Eleutherococcus sessiliflorus is a medicinal shrub widely used in East Asian traditional medicine, yet field-based studies on environmental influences remain limited. In this study, branches from 26 cultivation sites across South Korea were analyzed for relationships among growth traits, soil and climatic conditions, and two major compounds, chlorogenic acid (CGA) and eleutheroside E (EleuE). Growth traits varied widely, with plant height ranging from 1.06 to 4.20 m. CGA content was relatively stable across sites (0.292–0.708 mg/g), while EleuE showed greater variability (0.038–0.264 mg/g). The combined content of CGA and EleuE showed a weak positive correlation with thorn density (r = 0.236, p = 0.037). Plant height and basal diameter were positively correlated with temperature indices (annual average temperature r = 0.410, p < 0.001; annual maximum temperature r = 0.341, p = 0.002), whereas thorn density decreased with soil electrical conductivity, potassium, and magnesium but increased with sand and precipitation. Principal component analysis and correlation networks highlighted distinct clusters separating growth traits from EleuE–environment associations. These findings demonstrate that growth performance in E. sessiliflorus is strongly influenced by thermal regimes, while EleuE accumulation responds to soil texture and light availability, providing an empirical foundation for site-specific cultivation strategies and standardized quality management.