Yuji Isagi

Yuji Isagi


Research Focus & Metrics

Academic Career Length

37Years (1990-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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19 January 2010 in Journal of Biogeography
Verified
Journal Article
Closed access
PDF file
DBDavid M.J.S. Bowman
David M.J.S. Bowman
GBGillian Brown
Gillian Brown
MBM. F. Braby
M. F. Braby
JBJ. R. Brown
J. R. Brown
Lyn CookLyn Cook
Lyn Cook
PLP. Y. Ladiges
P. Y. Ladiges

1 July 1997 in Journal of Theoretical Biology
Verified
Journal Article
Closed access
YIYuji Isagi
Yuji Isagi
KSK. Sugimura
K. Sugimura
ASAkihiro Sumida
Akihiro Sumida
HIH. Ito
H. Ito

17 August 2020 in Environmental DNA
Verified
Journal Article
Open access
PDF file
HAHaruko Ando
Haruko Ando
HMHaruka Mukai
Haruka Mukai
TKTaketo Komura
Taketo Komura
TDTungga Dewi
Tungga Dewi
MAMasaki Ando
Masaki Ando
YIYuji Isagi
Yuji Isagi
Fine‐scale estimation of trophic interactions is an important subject in the field of ecology. Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive, accurate, and time‐ and cost‐effective tool to determine animal diets. Here, we summarize the trends of fecal metabarcoding studies as well as methodological characteristics using 155 original papers published from 2009 to March 2020. We calculated the frequencies of the methods and conditions used in each experimental procedure and bioinformatics approach. Mammals were the major target taxa for fecal metabarcoding. A few methods or conditions dominated each procedure: sampling, DNA extraction, PCR, sequencing, and bioinformatics, which might be specialized for metabarcoding of degraded fecal DNA. However, the disadvantages of common methods were noted in some studies, and further optimizations are required to obtain more accurate dietary data with high taxonomic resolution and quantitative performance. This review will help fecal metabarcode users, especially new scientists who are considering using fecal metabarcoding in their studies, understand the process and common methods of fecal metabarcoding. We also hope this review will facilitate further technical improvements in this method.

1 May 1997 in Plant Ecology
Verified
Journal Article
Closed access
PDF file
YIYuji Isagi
Yuji Isagi
TKT. Kawahara
T. Kawahara
KKK. Kamo
K. Kamo
HIH. Ito
H. Ito

31 May 2012 in Molecular Ecology
Verified
Journal Article
Closed access
PDF file
SSShota Sakaguchi
Shota Sakaguchi
YQYing‐Xiong Qiu
Ying‐Xiong Qiu
YLYi‐Hui Liu
Yi‐Hui Liu
XQXin‐Shuai Qi
Xin‐Shuai Qi
SKSea‐Hyun Kim
Sea‐Hyun Kim
YIYuji Isagi
Yuji Isagi

28 October 2015 in Tree Genetics & Genomes
Verified
Journal Article
Closed access
PDF file
SSShota Sakaguchi
Shota Sakaguchi
TSTakeshi Sugino
Takeshi Sugino
YTYoshihiko Tsumura
Yoshihiko Tsumura
MIMotomi Ito
Motomi Ito
MCMichael D. Crisp
Michael D. Crisp
YIYuji Isagi
Yuji Isagi