The shear effects on the anchorage interfaces under seismic action is a key problem requiring urgent investigation in the field of rock and soil anchorages. In this paper, the model of rock slope with a weak layer was constructed by pouring, and the large-scale shaking table model test was completed. The shear strain on the anchorage interfaces and the acceleration of the slope were collected using built measurement systems. The shear effects on the two anchorage interfaces (a bolt-grout interface and a grout-rock interface) and seismic responses of the slope under seismic action were investigated. The distribution laws of the shear stress on the two anchorage interfaces along the axial direction of the bolt under seismic action were gained. The variations of the peak acceleration amplification coefficient on the slope surface, the magnitude, and the growth rate of peak shear stress on the anchorage interfaces under seismic action with different excitation directions and intensities were obtained. Furthermore, the positive relationship between the shear effect on the anchorage interfaces and the seismic response of slope was revealed. This study provides support for theoretical research, numerical simulation analysis, and aseismic design of rock and soil anchorages under dynamic conditions.